Enhanced production of Shiga toxin 1 in enterohaemorrhagic Escherichia coli by oxygen

Enhanced production of Shiga toxin 1 in enterohaemorrhagic Escherichia coli by oxygen

Enterohaemorrhagic (EHEC) produces Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2). Although and were found within the late operons of the Stx-encoding phages (Stx-phages), could mainly be transcribed from the promoter ( ), which represents the functional operator-binding site (Fur box) for the transc...

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Veröffentlicht in:Microbiology (Society for General Microbiology) 2021-12, Vol.167 (12)
Hauptverfasser: Shimizu, Takeshi, Onuki, Manami, Suzuki, Shin, Hirai, Shinichiro, Yokoyama, Eiji, Matsumoto, Akio, Hamabata, Takashi
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container_issue 12
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container_title Microbiology (Society for General Microbiology)
container_volume 167
creator Shimizu, Takeshi
Onuki, Manami
Suzuki, Shin
Hirai, Shinichiro
Yokoyama, Eiji
Matsumoto, Akio
Hamabata, Takashi
description Enterohaemorrhagic (EHEC) produces Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2). Although and were found within the late operons of the Stx-encoding phages (Stx-phages), could mainly be transcribed from the promoter ( ), which represents the functional operator-binding site (Fur box) for the transcriptional regulator Fur (ferric uptake regulator), upstream of . In this study, we found that the production of Stx1 by EHEC was affected by oxygen concentration. Increased Stx1 production in the presence of oxygen is dependent on Fur, which is an Fe -responsive transcription factor. The intracellular Fe pool was lower under microaerobic conditions than under anaerobic conditions, suggesting that lower Fe availability drove the formation of less Fe -Fur, less DNA binding to the region, and an increase in Stx1 production.
doi_str_mv 10.1099/mic.0.001122
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title Enhanced production of Shiga toxin 1 in enterohaemorrhagic Escherichia coli by oxygen
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