Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis
Objectives To assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture. Materials and Methods Dogs with progressive ulcerative keratitis were prospectively recru...
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| Veröffentlicht in: | Journal of small animal practice 2022-03, Vol.63 (3), p.188-196 |
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| container_title | Journal of small animal practice |
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| creator | Hamzianpour, N. Adams, V. J. Grundon, R. A. Linn‐Pearl, R. Scurrell, E. Rozmanec, M. Civello, A. Goss, R. Watkins, C. Kearns, H. Heinrich, C. |
| description | Objectives
To assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture.
Materials and Methods
Dogs with progressive ulcerative keratitis were prospectively recruited for corneal cytology and culture. Corneal cytology slides were reviewed by veterinary surgeons of different training levels (three general practitioners, three ophthalmologists and three pathologists). The inter‐rater agreement of cytology findings and agreement of cytology with culture was assessed using the kappa measure of agreement.
Results
The study included 145 corneal cytology samples from 143 dogs (145 eyes) with progressive ulcerative keratitis. Positive cultures were obtained from 81 of 145 (56%) eyes. The most commonly isolated pathogens were Streptococcus canis, Pseudomonas aeruginosa and Staphylococcus pseudintermedius. The results demonstrated increased inter‐rater agreement of corneal cytology and increased agreement with culture with increased ocular pathology expertise (pathologists > ophthalmologists > general practitioners).
Clinical Significance
This study provides important information about the diagnostic value of corneal cytology in canine ulcerative keratitis and the most common pathogens involved in such cases in the UK. Based on the results of this study, cytology findings should be interpreted in conjunction with the expertise of the observer. For maximal pathogen identification, both cytology and culture should be considered. |
| doi_str_mv | 10.1111/jsap.13462 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2613290053</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2613290053</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3572-8a4a1925032e701ebf7e6b64a50b50b13734eb6e8e7b66e9c9a3aee4aa87a1053</originalsourceid><addsrcrecordid>eNp9kctKxDAUhoMoOl42PoAE3IhQza1NuxwGrwgK6rqcZk7HjJ10TFpldj6Cz-iTGB114cJw4OSQj49DfkJ2OTvi8RxPA8yPuFSZWCEDrlWepLnQq2TAmBCJSjnbIJshTOOYKc3WyYZUhdRc5APyNAwBQ5ih62hb0-4BqXUd-vfXNw-xU5h4xJ9n03qH0FCz6NqmnSwouDE1fdP1Hmlt3di6SYgCasBZh7RvDEaNfUb6-HXpbNgmazU0AXe--xa5Pz25G50nV9dnF6PhVWJkqkWSgwJeiJRJgZpxrGqNWZUpSFkVi0stFVYZ5qirLMPCFCABUQHkGjhL5RY5WHrnvn3qMXTlzAaDTQMO2z6UIuNSFCySEd3_g07b3ru4XaSkFhHkOlKHS8r4NgSPdTn3dgZ-UXJWfgZRfgZRfgUR4b1vZV_NcPyL_vx8BPgSeLENLv5RlZe3w5ul9AMJYZVD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2637213217</pqid></control><display><type>article</type><title>Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Hamzianpour, N. ; Adams, V. J. ; Grundon, R. A. ; Linn‐Pearl, R. ; Scurrell, E. ; Rozmanec, M. ; Civello, A. ; Goss, R. ; Watkins, C. ; Kearns, H. ; Heinrich, C.</creator><creatorcontrib>Hamzianpour, N. ; Adams, V. J. ; Grundon, R. A. ; Linn‐Pearl, R. ; Scurrell, E. ; Rozmanec, M. ; Civello, A. ; Goss, R. ; Watkins, C. ; Kearns, H. ; Heinrich, C.</creatorcontrib><description>Objectives
To assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture.
Materials and Methods
Dogs with progressive ulcerative keratitis were prospectively recruited for corneal cytology and culture. Corneal cytology slides were reviewed by veterinary surgeons of different training levels (three general practitioners, three ophthalmologists and three pathologists). The inter‐rater agreement of cytology findings and agreement of cytology with culture was assessed using the kappa measure of agreement.
Results
The study included 145 corneal cytology samples from 143 dogs (145 eyes) with progressive ulcerative keratitis. Positive cultures were obtained from 81 of 145 (56%) eyes. The most commonly isolated pathogens were Streptococcus canis, Pseudomonas aeruginosa and Staphylococcus pseudintermedius. The results demonstrated increased inter‐rater agreement of corneal cytology and increased agreement with culture with increased ocular pathology expertise (pathologists > ophthalmologists > general practitioners).
Clinical Significance
This study provides important information about the diagnostic value of corneal cytology in canine ulcerative keratitis and the most common pathogens involved in such cases in the UK. Based on the results of this study, cytology findings should be interpreted in conjunction with the expertise of the observer. For maximal pathogen identification, both cytology and culture should be considered.</description><identifier>ISSN: 0022-4510</identifier><identifier>EISSN: 1748-5827</identifier><identifier>DOI: 10.1111/jsap.13462</identifier><identifier>PMID: 34937128</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agreements ; Animals ; Cellular biology ; Cornea ; Cornea - pathology ; Corneal Ulcer - diagnosis ; Corneal Ulcer - pathology ; Corneal Ulcer - veterinary ; Cytology ; Dog Diseases - diagnosis ; Dog Diseases - pathology ; Dogs ; Keratitis ; Medical personnel ; Pathogens ; Pathology ; Veterinary surgeons</subject><ispartof>Journal of small animal practice, 2022-03, Vol.63 (3), p.188-196</ispartof><rights>2021 British Small Animal Veterinary Association</rights><rights>2021 British Small Animal Veterinary Association.</rights><rights>2022 British Small Animal Veterinary Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3572-8a4a1925032e701ebf7e6b64a50b50b13734eb6e8e7b66e9c9a3aee4aa87a1053</citedby><cites>FETCH-LOGICAL-c3572-8a4a1925032e701ebf7e6b64a50b50b13734eb6e8e7b66e9c9a3aee4aa87a1053</cites><orcidid>0000-0001-7874-8252 ; 0000-0001-7353-5098</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjsap.13462$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjsap.13462$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34937128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamzianpour, N.</creatorcontrib><creatorcontrib>Adams, V. J.</creatorcontrib><creatorcontrib>Grundon, R. A.</creatorcontrib><creatorcontrib>Linn‐Pearl, R.</creatorcontrib><creatorcontrib>Scurrell, E.</creatorcontrib><creatorcontrib>Rozmanec, M.</creatorcontrib><creatorcontrib>Civello, A.</creatorcontrib><creatorcontrib>Goss, R.</creatorcontrib><creatorcontrib>Watkins, C.</creatorcontrib><creatorcontrib>Kearns, H.</creatorcontrib><creatorcontrib>Heinrich, C.</creatorcontrib><title>Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis</title><title>Journal of small animal practice</title><addtitle>J Small Anim Pract</addtitle><description>Objectives
To assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture.
Materials and Methods
Dogs with progressive ulcerative keratitis were prospectively recruited for corneal cytology and culture. Corneal cytology slides were reviewed by veterinary surgeons of different training levels (three general practitioners, three ophthalmologists and three pathologists). The inter‐rater agreement of cytology findings and agreement of cytology with culture was assessed using the kappa measure of agreement.
Results
The study included 145 corneal cytology samples from 143 dogs (145 eyes) with progressive ulcerative keratitis. Positive cultures were obtained from 81 of 145 (56%) eyes. The most commonly isolated pathogens were Streptococcus canis, Pseudomonas aeruginosa and Staphylococcus pseudintermedius. The results demonstrated increased inter‐rater agreement of corneal cytology and increased agreement with culture with increased ocular pathology expertise (pathologists > ophthalmologists > general practitioners).
Clinical Significance
This study provides important information about the diagnostic value of corneal cytology in canine ulcerative keratitis and the most common pathogens involved in such cases in the UK. Based on the results of this study, cytology findings should be interpreted in conjunction with the expertise of the observer. For maximal pathogen identification, both cytology and culture should be considered.</description><subject>Agreements</subject><subject>Animals</subject><subject>Cellular biology</subject><subject>Cornea</subject><subject>Cornea - pathology</subject><subject>Corneal Ulcer - diagnosis</subject><subject>Corneal Ulcer - pathology</subject><subject>Corneal Ulcer - veterinary</subject><subject>Cytology</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - pathology</subject><subject>Dogs</subject><subject>Keratitis</subject><subject>Medical personnel</subject><subject>Pathogens</subject><subject>Pathology</subject><subject>Veterinary surgeons</subject><issn>0022-4510</issn><issn>1748-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctKxDAUhoMoOl42PoAE3IhQza1NuxwGrwgK6rqcZk7HjJ10TFpldj6Cz-iTGB114cJw4OSQj49DfkJ2OTvi8RxPA8yPuFSZWCEDrlWepLnQq2TAmBCJSjnbIJshTOOYKc3WyYZUhdRc5APyNAwBQ5ih62hb0-4BqXUd-vfXNw-xU5h4xJ9n03qH0FCz6NqmnSwouDE1fdP1Hmlt3di6SYgCasBZh7RvDEaNfUb6-HXpbNgmazU0AXe--xa5Pz25G50nV9dnF6PhVWJkqkWSgwJeiJRJgZpxrGqNWZUpSFkVi0stFVYZ5qirLMPCFCABUQHkGjhL5RY5WHrnvn3qMXTlzAaDTQMO2z6UIuNSFCySEd3_g07b3ru4XaSkFhHkOlKHS8r4NgSPdTn3dgZ-UXJWfgZRfgZRfgUR4b1vZV_NcPyL_vx8BPgSeLENLv5RlZe3w5ul9AMJYZVD</recordid><startdate>202203</startdate><enddate>202203</enddate><creator>Hamzianpour, N.</creator><creator>Adams, V. J.</creator><creator>Grundon, R. A.</creator><creator>Linn‐Pearl, R.</creator><creator>Scurrell, E.</creator><creator>Rozmanec, M.</creator><creator>Civello, A.</creator><creator>Goss, R.</creator><creator>Watkins, C.</creator><creator>Kearns, H.</creator><creator>Heinrich, C.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7874-8252</orcidid><orcidid>https://orcid.org/0000-0001-7353-5098</orcidid></search><sort><creationdate>202203</creationdate><title>Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis</title><author>Hamzianpour, N. ; Adams, V. J. ; Grundon, R. A. ; Linn‐Pearl, R. ; Scurrell, E. ; Rozmanec, M. ; Civello, A. ; Goss, R. ; Watkins, C. ; Kearns, H. ; Heinrich, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3572-8a4a1925032e701ebf7e6b64a50b50b13734eb6e8e7b66e9c9a3aee4aa87a1053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agreements</topic><topic>Animals</topic><topic>Cellular biology</topic><topic>Cornea</topic><topic>Cornea - pathology</topic><topic>Corneal Ulcer - diagnosis</topic><topic>Corneal Ulcer - pathology</topic><topic>Corneal Ulcer - veterinary</topic><topic>Cytology</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - pathology</topic><topic>Dogs</topic><topic>Keratitis</topic><topic>Medical personnel</topic><topic>Pathogens</topic><topic>Pathology</topic><topic>Veterinary surgeons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamzianpour, N.</creatorcontrib><creatorcontrib>Adams, V. J.</creatorcontrib><creatorcontrib>Grundon, R. A.</creatorcontrib><creatorcontrib>Linn‐Pearl, R.</creatorcontrib><creatorcontrib>Scurrell, E.</creatorcontrib><creatorcontrib>Rozmanec, M.</creatorcontrib><creatorcontrib>Civello, A.</creatorcontrib><creatorcontrib>Goss, R.</creatorcontrib><creatorcontrib>Watkins, C.</creatorcontrib><creatorcontrib>Kearns, H.</creatorcontrib><creatorcontrib>Heinrich, C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of small animal practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamzianpour, N.</au><au>Adams, V. J.</au><au>Grundon, R. A.</au><au>Linn‐Pearl, R.</au><au>Scurrell, E.</au><au>Rozmanec, M.</au><au>Civello, A.</au><au>Goss, R.</au><au>Watkins, C.</au><au>Kearns, H.</au><au>Heinrich, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis</atitle><jtitle>Journal of small animal practice</jtitle><addtitle>J Small Anim Pract</addtitle><date>2022-03</date><risdate>2022</risdate><volume>63</volume><issue>3</issue><spage>188</spage><epage>196</epage><pages>188-196</pages><issn>0022-4510</issn><eissn>1748-5827</eissn><abstract>Objectives
To assess the inter‐rater agreement of corneal cytology findings in canine ulcerative keratitis by veterinary surgeons of different training levels and the agreement of corneal cytology with culture.
Materials and Methods
Dogs with progressive ulcerative keratitis were prospectively recruited for corneal cytology and culture. Corneal cytology slides were reviewed by veterinary surgeons of different training levels (three general practitioners, three ophthalmologists and three pathologists). The inter‐rater agreement of cytology findings and agreement of cytology with culture was assessed using the kappa measure of agreement.
Results
The study included 145 corneal cytology samples from 143 dogs (145 eyes) with progressive ulcerative keratitis. Positive cultures were obtained from 81 of 145 (56%) eyes. The most commonly isolated pathogens were Streptococcus canis, Pseudomonas aeruginosa and Staphylococcus pseudintermedius. The results demonstrated increased inter‐rater agreement of corneal cytology and increased agreement with culture with increased ocular pathology expertise (pathologists > ophthalmologists > general practitioners).
Clinical Significance
This study provides important information about the diagnostic value of corneal cytology in canine ulcerative keratitis and the most common pathogens involved in such cases in the UK. Based on the results of this study, cytology findings should be interpreted in conjunction with the expertise of the observer. For maximal pathogen identification, both cytology and culture should be considered.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>34937128</pmid><doi>10.1111/jsap.13462</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7874-8252</orcidid><orcidid>https://orcid.org/0000-0001-7353-5098</orcidid></addata></record> |
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| ispartof | Journal of small animal practice, 2022-03, Vol.63 (3), p.188-196 |
| issn | 0022-4510 1748-5827 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Agreements Animals Cellular biology Cornea Cornea - pathology Corneal Ulcer - diagnosis Corneal Ulcer - pathology Corneal Ulcer - veterinary Cytology Dog Diseases - diagnosis Dog Diseases - pathology Dogs Keratitis Medical personnel Pathogens Pathology Veterinary surgeons |
| title | Assessment of the inter‐rater agreement of corneal cytology and culture findings in canine ulcerative keratitis |
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