Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana
[Display omitted] •B. bassiana contains bioactive lipids with strong anti-platelet effects.•B. bassiana bioactive lipids were assessed against platelet activating factor (PAF).•HPLC-analysis elucidated that the most bioactive were polar lipids (PLs) like phosphatidylcholines.•Some cells’ bioactive P...
Gespeichert in:
| Veröffentlicht in: | Prostaglandins & other lipid mediators 2022-02, Vol.158, p.106606-106606, Article 106606 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 106606 |
|---|---|
| container_issue | |
| container_start_page | 106606 |
| container_title | Prostaglandins & other lipid mediators |
| container_volume | 158 |
| creator | Tsoupras, Alexandros Kouvelis, Vassili N. Pappas, Katherine M. Demopoulos, Constantinos A. Typas, Milton A. |
| description | [Display omitted]
•B. bassiana contains bioactive lipids with strong anti-platelet effects.•B. bassiana bioactive lipids were assessed against platelet activating factor (PAF).•HPLC-analysis elucidated that the most bioactive were polar lipids (PLs) like phosphatidylcholines.•Some cells’ bioactive PLs are secreted in the culture-medium, while others not.•B. bassiana cultures emerge as a sustainable source of anti-inflammatory bioactive ingredients.
In the present work the entomopathogenic fungus B. bassiana lipids were studied against the potent pro-inflammatory and thrombotic mediators implicated in several disorders, platelet-activating factor (PAF) and thrombin. Bioactivities of lipid extracts from B. bassiana cells and culture supernatants and of their lipid fractions separated by a one-step HPLC-analysis ere assessed against the PAF/Thrombin-induced aggregation of washed rabbit platelets. Lipid extracts from both cell-biomass and supernatant inhibited strongly PAF/Thrombin-activities and platelet-aggregation, exhibiting higher specificity against PAF. Similarly, HPLC-derived lipid-fractions of phenolics/glycolipids, Sphingomyelins and Phosphatidylcholines (PC) showed strong anti-PAF potency. PC PAF-like molecules exhibited the strongest antagonistic anti-PAF effects, while in higher amounts they agonistically inhibited PAF-activities. Some bioactive lipids with strong anti-PAF effects are exo-cellularly secreted in the culture media during fungal growth, while others are not. The presence of such lipid bioactives in B. bassiana with strong anti-inflammatory and anti-thrombotic properties, provide new perspectives and putative future applications for this entomopathogenic fungus. |
| doi_str_mv | 10.1016/j.prostaglandins.2021.106606 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2612047535</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1098882321000812</els_id><sourcerecordid>2612047535</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-2ca3fc121c8c2e4fffb5e478bead5dbf80ae3329bc34867d4fc6e6fa2f81271b3</originalsourceid><addsrcrecordid>eNqNkD9vFDEQxV2ASAh8BeSCgmYv_rPr80k0ISIEKRIN1JbtHd_NaddebO-JfHscXUCioxiNNPPePM2PkPecbTjj6vq4WXIq1e4nG0eMZSOY4G2lFFMvyCVnO91pLeQFeV3KkTHBOGevyIXsd0LyQVySXzexYocxTHaebU35kbZTrdq0HnKaXaroaYtZIFeEQlOgEy44UofJ-oqnNgtNSOsBKMSa5rTYekh7iM0Y1rhfC_0Edj1BRkudLQVttG_Iy2CnAm-f-xX5cff5--199_Dty9fbm4fOS61qJ7yVwXPBvfYC-hCCG6Dfagd2HEYXNLMgpdg5L3uttmMfvAIVrAiaiy138op8ON9tL_xcoVQzY_EwNWKQ1mKE4oL120EOTfrxLPUNaskQzJJxtvnRcGaeeJuj-Ze3eeJtzryb_d1z0upmGP-a_8BugruzANq_J4RsikeIHkbM4KsZE_5f0m89AKDS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2612047535</pqid></control><display><type>article</type><title>Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tsoupras, Alexandros ; Kouvelis, Vassili N. ; Pappas, Katherine M. ; Demopoulos, Constantinos A. ; Typas, Milton A.</creator><creatorcontrib>Tsoupras, Alexandros ; Kouvelis, Vassili N. ; Pappas, Katherine M. ; Demopoulos, Constantinos A. ; Typas, Milton A.</creatorcontrib><description>[Display omitted]
•B. bassiana contains bioactive lipids with strong anti-platelet effects.•B. bassiana bioactive lipids were assessed against platelet activating factor (PAF).•HPLC-analysis elucidated that the most bioactive were polar lipids (PLs) like phosphatidylcholines.•Some cells’ bioactive PLs are secreted in the culture-medium, while others not.•B. bassiana cultures emerge as a sustainable source of anti-inflammatory bioactive ingredients.
In the present work the entomopathogenic fungus B. bassiana lipids were studied against the potent pro-inflammatory and thrombotic mediators implicated in several disorders, platelet-activating factor (PAF) and thrombin. Bioactivities of lipid extracts from B. bassiana cells and culture supernatants and of their lipid fractions separated by a one-step HPLC-analysis ere assessed against the PAF/Thrombin-induced aggregation of washed rabbit platelets. Lipid extracts from both cell-biomass and supernatant inhibited strongly PAF/Thrombin-activities and platelet-aggregation, exhibiting higher specificity against PAF. Similarly, HPLC-derived lipid-fractions of phenolics/glycolipids, Sphingomyelins and Phosphatidylcholines (PC) showed strong anti-PAF potency. PC PAF-like molecules exhibited the strongest antagonistic anti-PAF effects, while in higher amounts they agonistically inhibited PAF-activities. Some bioactive lipids with strong anti-PAF effects are exo-cellularly secreted in the culture media during fungal growth, while others are not. The presence of such lipid bioactives in B. bassiana with strong anti-inflammatory and anti-thrombotic properties, provide new perspectives and putative future applications for this entomopathogenic fungus.</description><identifier>ISSN: 1098-8823</identifier><identifier>DOI: 10.1016/j.prostaglandins.2021.106606</identifier><identifier>PMID: 34923152</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Anti-inflammatory ; Anti-Inflammatory Agents - pharmacology ; Antithrombotic ; Beauveria ; Beauveria bassiana ; Chromatography, High Pressure Liquid ; Inflammation ; Lipid bioactives ; PAF ; Platelet Activating Factor - pharmacology ; Platelet Aggregation ; Platelets ; Rabbits</subject><ispartof>Prostaglandins & other lipid mediators, 2022-02, Vol.158, p.106606-106606, Article 106606</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-2ca3fc121c8c2e4fffb5e478bead5dbf80ae3329bc34867d4fc6e6fa2f81271b3</citedby><cites>FETCH-LOGICAL-c386t-2ca3fc121c8c2e4fffb5e478bead5dbf80ae3329bc34867d4fc6e6fa2f81271b3</cites><orcidid>0000-0001-6753-0872 ; 0000-0002-0837-9778</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.prostaglandins.2021.106606$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34923152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsoupras, Alexandros</creatorcontrib><creatorcontrib>Kouvelis, Vassili N.</creatorcontrib><creatorcontrib>Pappas, Katherine M.</creatorcontrib><creatorcontrib>Demopoulos, Constantinos A.</creatorcontrib><creatorcontrib>Typas, Milton A.</creatorcontrib><title>Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana</title><title>Prostaglandins & other lipid mediators</title><addtitle>Prostaglandins Other Lipid Mediat</addtitle><description>[Display omitted]
•B. bassiana contains bioactive lipids with strong anti-platelet effects.•B. bassiana bioactive lipids were assessed against platelet activating factor (PAF).•HPLC-analysis elucidated that the most bioactive were polar lipids (PLs) like phosphatidylcholines.•Some cells’ bioactive PLs are secreted in the culture-medium, while others not.•B. bassiana cultures emerge as a sustainable source of anti-inflammatory bioactive ingredients.
In the present work the entomopathogenic fungus B. bassiana lipids were studied against the potent pro-inflammatory and thrombotic mediators implicated in several disorders, platelet-activating factor (PAF) and thrombin. Bioactivities of lipid extracts from B. bassiana cells and culture supernatants and of their lipid fractions separated by a one-step HPLC-analysis ere assessed against the PAF/Thrombin-induced aggregation of washed rabbit platelets. Lipid extracts from both cell-biomass and supernatant inhibited strongly PAF/Thrombin-activities and platelet-aggregation, exhibiting higher specificity against PAF. Similarly, HPLC-derived lipid-fractions of phenolics/glycolipids, Sphingomyelins and Phosphatidylcholines (PC) showed strong anti-PAF potency. PC PAF-like molecules exhibited the strongest antagonistic anti-PAF effects, while in higher amounts they agonistically inhibited PAF-activities. Some bioactive lipids with strong anti-PAF effects are exo-cellularly secreted in the culture media during fungal growth, while others are not. The presence of such lipid bioactives in B. bassiana with strong anti-inflammatory and anti-thrombotic properties, provide new perspectives and putative future applications for this entomopathogenic fungus.</description><subject>Animals</subject><subject>Anti-inflammatory</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antithrombotic</subject><subject>Beauveria</subject><subject>Beauveria bassiana</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Inflammation</subject><subject>Lipid bioactives</subject><subject>PAF</subject><subject>Platelet Activating Factor - pharmacology</subject><subject>Platelet Aggregation</subject><subject>Platelets</subject><subject>Rabbits</subject><issn>1098-8823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkD9vFDEQxV2ASAh8BeSCgmYv_rPr80k0ISIEKRIN1JbtHd_NaddebO-JfHscXUCioxiNNPPePM2PkPecbTjj6vq4WXIq1e4nG0eMZSOY4G2lFFMvyCVnO91pLeQFeV3KkTHBOGevyIXsd0LyQVySXzexYocxTHaebU35kbZTrdq0HnKaXaroaYtZIFeEQlOgEy44UofJ-oqnNgtNSOsBKMSa5rTYekh7iM0Y1rhfC_0Edj1BRkudLQVttG_Iy2CnAm-f-xX5cff5--199_Dty9fbm4fOS61qJ7yVwXPBvfYC-hCCG6Dfagd2HEYXNLMgpdg5L3uttmMfvAIVrAiaiy138op8ON9tL_xcoVQzY_EwNWKQ1mKE4oL120EOTfrxLPUNaskQzJJxtvnRcGaeeJuj-Ze3eeJtzryb_d1z0upmGP-a_8BugruzANq_J4RsikeIHkbM4KsZE_5f0m89AKDS</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Tsoupras, Alexandros</creator><creator>Kouvelis, Vassili N.</creator><creator>Pappas, Katherine M.</creator><creator>Demopoulos, Constantinos A.</creator><creator>Typas, Milton A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6753-0872</orcidid><orcidid>https://orcid.org/0000-0002-0837-9778</orcidid></search><sort><creationdate>202202</creationdate><title>Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana</title><author>Tsoupras, Alexandros ; Kouvelis, Vassili N. ; Pappas, Katherine M. ; Demopoulos, Constantinos A. ; Typas, Milton A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-2ca3fc121c8c2e4fffb5e478bead5dbf80ae3329bc34867d4fc6e6fa2f81271b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Anti-inflammatory</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Antithrombotic</topic><topic>Beauveria</topic><topic>Beauveria bassiana</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Inflammation</topic><topic>Lipid bioactives</topic><topic>PAF</topic><topic>Platelet Activating Factor - pharmacology</topic><topic>Platelet Aggregation</topic><topic>Platelets</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsoupras, Alexandros</creatorcontrib><creatorcontrib>Kouvelis, Vassili N.</creatorcontrib><creatorcontrib>Pappas, Katherine M.</creatorcontrib><creatorcontrib>Demopoulos, Constantinos A.</creatorcontrib><creatorcontrib>Typas, Milton A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prostaglandins & other lipid mediators</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsoupras, Alexandros</au><au>Kouvelis, Vassili N.</au><au>Pappas, Katherine M.</au><au>Demopoulos, Constantinos A.</au><au>Typas, Milton A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana</atitle><jtitle>Prostaglandins & other lipid mediators</jtitle><addtitle>Prostaglandins Other Lipid Mediat</addtitle><date>2022-02</date><risdate>2022</risdate><volume>158</volume><spage>106606</spage><epage>106606</epage><pages>106606-106606</pages><artnum>106606</artnum><issn>1098-8823</issn><abstract>[Display omitted]
•B. bassiana contains bioactive lipids with strong anti-platelet effects.•B. bassiana bioactive lipids were assessed against platelet activating factor (PAF).•HPLC-analysis elucidated that the most bioactive were polar lipids (PLs) like phosphatidylcholines.•Some cells’ bioactive PLs are secreted in the culture-medium, while others not.•B. bassiana cultures emerge as a sustainable source of anti-inflammatory bioactive ingredients.
In the present work the entomopathogenic fungus B. bassiana lipids were studied against the potent pro-inflammatory and thrombotic mediators implicated in several disorders, platelet-activating factor (PAF) and thrombin. Bioactivities of lipid extracts from B. bassiana cells and culture supernatants and of their lipid fractions separated by a one-step HPLC-analysis ere assessed against the PAF/Thrombin-induced aggregation of washed rabbit platelets. Lipid extracts from both cell-biomass and supernatant inhibited strongly PAF/Thrombin-activities and platelet-aggregation, exhibiting higher specificity against PAF. Similarly, HPLC-derived lipid-fractions of phenolics/glycolipids, Sphingomyelins and Phosphatidylcholines (PC) showed strong anti-PAF potency. PC PAF-like molecules exhibited the strongest antagonistic anti-PAF effects, while in higher amounts they agonistically inhibited PAF-activities. Some bioactive lipids with strong anti-PAF effects are exo-cellularly secreted in the culture media during fungal growth, while others are not. The presence of such lipid bioactives in B. bassiana with strong anti-inflammatory and anti-thrombotic properties, provide new perspectives and putative future applications for this entomopathogenic fungus.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34923152</pmid><doi>10.1016/j.prostaglandins.2021.106606</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-6753-0872</orcidid><orcidid>https://orcid.org/0000-0002-0837-9778</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1098-8823 |
| ispartof | Prostaglandins & other lipid mediators, 2022-02, Vol.158, p.106606-106606, Article 106606 |
| issn | 1098-8823 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2612047535 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Anti-inflammatory Anti-Inflammatory Agents - pharmacology Antithrombotic Beauveria Beauveria bassiana Chromatography, High Pressure Liquid Inflammation Lipid bioactives PAF Platelet Activating Factor - pharmacology Platelet Aggregation Platelets Rabbits |
| title | Anti-inflammatory and anti-thrombotic properties of lipid bioactives from the entomopathogenic fungus Beauveria bassiana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T14%3A50%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anti-inflammatory%20and%20anti-thrombotic%20properties%20of%20lipid%20bioactives%20from%20the%20entomopathogenic%20fungus%20Beauveria%20bassiana&rft.jtitle=Prostaglandins%20&%20other%20lipid%20mediators&rft.au=Tsoupras,%20Alexandros&rft.date=2022-02&rft.volume=158&rft.spage=106606&rft.epage=106606&rft.pages=106606-106606&rft.artnum=106606&rft.issn=1098-8823&rft_id=info:doi/10.1016/j.prostaglandins.2021.106606&rft_dat=%3Cproquest_cross%3E2612047535%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2612047535&rft_id=info:pmid/34923152&rft_els_id=S1098882321000812&rfr_iscdi=true |