Proteomics analysis of the effects for different salt ions in leaves of true halophyte Sesuvium portulacastrum

Sesuvium portulacastrum is a true halophyte and shows an optimal development under moderate salinity with large amounts of salt ions in its leaves. However, the specific proteins in response to salt ions are remained unknown. In this study, comparative physiological and proteomic analyses of different leaves subject to NaCl, KCl, NaNO3 and KNO3 were performed. Chlorophyll content was decreased under the above four kinds of salt treatments. Starch and soluble sugar contents changed differently under different salt treatments. A total of 53 differentially accumulated proteins (DAPs) were identified by mass spectrometry. Among them, 13, 25, 26 and 25 DAPs were identified after exposure to KCl, NaCl, KNO3, and NaNO3, respectively. These DAPs belong to 47 unique genes, and 37 of them are involved in protein-protein interactions. These DAPs displayed different expression patterns after treating with different salt ions. Functional annotation revealed they are mainly involved in photosynthesis, carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Genes and proteins showed different expression profiles under different salt treatments. Enzyme activity analysis indicated P-ATPase was induced by KCl, NaCl and NaNO3, V-ATPase was induced by KCl and NaCl, whereas V-PPase activity was significantly increased after application of KNO3, but sharply inhibited by NaCl. These results might deepen our understanding of responsive mechanisms in the leaves of S. portulacastrum upon different salt ions. [Display omitted] •Proteomics of Sesuvium portulacastrum leaves subjected to NaCl, KCl, NaNO3 and KNO3 were performed.•A total of 53 differentially accumulated proteins (DAPs) upon different salt ions were identified by mass spectrometry.•Genes and proteins showed different expression profiles under different salt treatments in S. portulacastrum leaves.•Activities of P-ATPase and V-PPase were induced by KCl and NaCl, but V-PPase activity was significantly increased upon KNO3.•Proteins in S. Portulacastrum leaves are more sensitive to Na + than K+ and Cl−.