Protective effect of adrenomedullin on hyperoxia-induced lung injury
To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pul...
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| Veröffentlicht in: | Zhongguo dang dai er ke za zhi 2021-12, Vol.23 (12), p.1282-1288 |
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| container_title | Zhongguo dang dai er ke za zhi |
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| creator | Zhang, Min Cheng, Li-Hua Yin, Xiao-Tong Luo, Hao Cai, Cheng |
| description | To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pulmonary microvascular endothelial cells (HPMECs) under different experimental conditions.
HPMECs were randomly divided into an air group and a hyperoxia group (
=3 each).The HPMECs in the hyperoxia group were cultured in an atmosphere of 92% O
(3 L/minute) +5% CO
. RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB. Other HPMECs were divided into a non-interference group and an interference group (
=3 each), and the mRNA and protein expression levels of ADM, ERK1/2, and PKB were measured after the HPMECs in the interference group were transfected with ADM siRNA.
Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB ( |
| doi_str_mv | 10.7499/j.issn.1008-8830.2109005 |
| format | Article |
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HPMECs were randomly divided into an air group and a hyperoxia group (
=3 each).The HPMECs in the hyperoxia group were cultured in an atmosphere of 92% O
(3 L/minute) +5% CO
. RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB. Other HPMECs were divided into a non-interference group and an interference group (
=3 each), and the mRNA and protein expression levels of ADM, ERK1/2, and PKB were measured after the HPMECs in the interference group were transfected with ADM siRNA.
Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB (
<0.05). Compared with the non-interference group, the interference group had significant reductions in the mRNA and protein expression levels of ADM, ERK1/2, and PKB (
<0.05).
ERK1/2 and PKB may be the downstream targets of the ADM signaling pathway. ADM mediates the ERK/PKB signaling pathway by regulating CRLR/RAMP2 and participates in the protection of hyperoxia-induced lung injury.</description><identifier>ISSN: 1008-8830</identifier><identifier>DOI: 10.7499/j.issn.1008-8830.2109005</identifier><identifier>PMID: 34911614</identifier><language>chi ; eng</language><publisher>China</publisher><subject>Adrenomedullin - genetics ; Endothelial Cells ; Humans ; Hyperoxia - complications ; Lung Injury ; Receptor Activity-Modifying Proteins</subject><ispartof>Zhongguo dang dai er ke za zhi, 2021-12, Vol.23 (12), p.1282-1288</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1114-4174e6c805f8c66115b25a7f666365051929b1132422b8dadeb6b1cd2132d5643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34911614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Cheng, Li-Hua</creatorcontrib><creatorcontrib>Yin, Xiao-Tong</creatorcontrib><creatorcontrib>Luo, Hao</creatorcontrib><creatorcontrib>Cai, Cheng</creatorcontrib><title>Protective effect of adrenomedullin on hyperoxia-induced lung injury</title><title>Zhongguo dang dai er ke za zhi</title><addtitle>Zhongguo Dang Dai Er Ke Za Zhi</addtitle><description>To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pulmonary microvascular endothelial cells (HPMECs) under different experimental conditions.
HPMECs were randomly divided into an air group and a hyperoxia group (
=3 each).The HPMECs in the hyperoxia group were cultured in an atmosphere of 92% O
(3 L/minute) +5% CO
. RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB. Other HPMECs were divided into a non-interference group and an interference group (
=3 each), and the mRNA and protein expression levels of ADM, ERK1/2, and PKB were measured after the HPMECs in the interference group were transfected with ADM siRNA.
Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB (
<0.05). Compared with the non-interference group, the interference group had significant reductions in the mRNA and protein expression levels of ADM, ERK1/2, and PKB (
<0.05).
ERK1/2 and PKB may be the downstream targets of the ADM signaling pathway. ADM mediates the ERK/PKB signaling pathway by regulating CRLR/RAMP2 and participates in the protection of hyperoxia-induced lung injury.</description><subject>Adrenomedullin - genetics</subject><subject>Endothelial Cells</subject><subject>Humans</subject><subject>Hyperoxia - complications</subject><subject>Lung Injury</subject><subject>Receptor Activity-Modifying Proteins</subject><issn>1008-8830</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9j0tLAzEYRbNQbH38BcnSzYz58mqylPqEgi50PWSSjKZMk5o0Yv-9A1ZX93K4XDgIYSDtgmt9vW5DKbEFQlSjFCMtBaIJEUdo_s9m6LSU9QQV1-wEzRjXABL4HN2-5LTzdhe-PPbDMDWcBmxc9jFtvKvjGCJOEX_stz6n72CaEF213uGxxncc4rrm_Tk6HsxY_MUhz9Db_d3r8rFZPT88LW9WjQUA3nBYcC-tImJQVkoA0VNhFoOUkklBBGiqewBGOaW9csb5XvZgHZ2QE5KzM3T1-7vN6bP6sus2oVg_jib6VEtH5aQOhFI6TS8P09pPHt02h43J--7PnP0Akg1aFw</recordid><startdate>20211215</startdate><enddate>20211215</enddate><creator>Zhang, Min</creator><creator>Cheng, Li-Hua</creator><creator>Yin, Xiao-Tong</creator><creator>Luo, Hao</creator><creator>Cai, Cheng</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20211215</creationdate><title>Protective effect of adrenomedullin on hyperoxia-induced lung injury</title><author>Zhang, Min ; Cheng, Li-Hua ; Yin, Xiao-Tong ; Luo, Hao ; Cai, Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1114-4174e6c805f8c66115b25a7f666365051929b1132422b8dadeb6b1cd2132d5643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>Adrenomedullin - genetics</topic><topic>Endothelial Cells</topic><topic>Humans</topic><topic>Hyperoxia - complications</topic><topic>Lung Injury</topic><topic>Receptor Activity-Modifying Proteins</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Cheng, Li-Hua</creatorcontrib><creatorcontrib>Yin, Xiao-Tong</creatorcontrib><creatorcontrib>Luo, Hao</creatorcontrib><creatorcontrib>Cai, Cheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhongguo dang dai er ke za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Min</au><au>Cheng, Li-Hua</au><au>Yin, Xiao-Tong</au><au>Luo, Hao</au><au>Cai, Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of adrenomedullin on hyperoxia-induced lung injury</atitle><jtitle>Zhongguo dang dai er ke za zhi</jtitle><addtitle>Zhongguo Dang Dai Er Ke Za Zhi</addtitle><date>2021-12-15</date><risdate>2021</risdate><volume>23</volume><issue>12</issue><spage>1282</spage><epage>1288</epage><pages>1282-1288</pages><issn>1008-8830</issn><abstract>To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pulmonary microvascular endothelial cells (HPMECs) under different experimental conditions.
HPMECs were randomly divided into an air group and a hyperoxia group (
=3 each).The HPMECs in the hyperoxia group were cultured in an atmosphere of 92% O
(3 L/minute) +5% CO
. RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB. Other HPMECs were divided into a non-interference group and an interference group (
=3 each), and the mRNA and protein expression levels of ADM, ERK1/2, and PKB were measured after the HPMECs in the interference group were transfected with ADM siRNA.
Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB (
<0.05). Compared with the non-interference group, the interference group had significant reductions in the mRNA and protein expression levels of ADM, ERK1/2, and PKB (
<0.05).
ERK1/2 and PKB may be the downstream targets of the ADM signaling pathway. ADM mediates the ERK/PKB signaling pathway by regulating CRLR/RAMP2 and participates in the protection of hyperoxia-induced lung injury.</abstract><cop>China</cop><pmid>34911614</pmid><doi>10.7499/j.issn.1008-8830.2109005</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 1008-8830 |
| ispartof | Zhongguo dang dai er ke za zhi, 2021-12, Vol.23 (12), p.1282-1288 |
| issn | 1008-8830 |
| language | chi ; eng |
| recordid | cdi_proquest_miscellaneous_2610910222 |
| source | MEDLINE; PubMed Central |
| subjects | Adrenomedullin - genetics Endothelial Cells Humans Hyperoxia - complications Lung Injury Receptor Activity-Modifying Proteins |
| title | Protective effect of adrenomedullin on hyperoxia-induced lung injury |
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