Microbiome Methods in Experimental Autoimmune Encephalomyelitis
Microbiome composition studies are increasingly shedding light on animal models of disease. This paper describes a protocol for analyzing the gut microbiome composition prior to and after the induction of mice to experimental autoimmune encephalomyelitis (EAE), the principal animal model of the huma...
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| Veröffentlicht in: | Current protocols 2021-12, Vol.1 (12), p.e314-n/a |
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| creator | Daberkow, David P. Hoffman, Kristina Kohl, Hannah M. Long, Tyrel Kirby, Trevor O. Ochoa‐Repáraz, Javier |
| description | Microbiome composition studies are increasingly shedding light on animal models of disease. This paper describes a protocol for analyzing the gut microbiome composition prior to and after the induction of mice to experimental autoimmune encephalomyelitis (EAE), the principal animal model of the human neuroinflammatory demyelinating disease multiple sclerosis (MS). We also address and provide data assessing the impact of mice reared in different animal facilities on EAE induction. Furthermore, we discuss potential regulators of the gut‐microbiome‐brain axis (GMBA) in relation to neuroinflammation and implications on demyelinating disease states. Our results suggest that mice reared in different animal facilities produce different levels of EAE induction. These results highlight the importance of accounting for consistent environmental conditions when inducing EAE and other animal models of disease. © 2021 Wiley Periodicals LLC.
Basic Protocol 1: Study of the composition of the gut microbiome in the neuroinflammatory model of experimental autoimmune encephalomyelitis
Basic Protocol 2: Experimental procedures for DNA extraction and microbiome analysis |
| doi_str_mv | 10.1002/cpz1.314 |
| format | Article |
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Basic Protocol 1: Study of the composition of the gut microbiome in the neuroinflammatory model of experimental autoimmune encephalomyelitis
Basic Protocol 2: Experimental procedures for DNA extraction and microbiome analysis</description><identifier>ISSN: 2691-1299</identifier><identifier>EISSN: 2691-1299</identifier><identifier>DOI: 10.1002/cpz1.314</identifier><language>eng</language><subject>EAE ; experimental autoimmune encephalomyelitis ; GMBA ; gut‐microbiome‐brain axis ; neuroinflammation ; neurotransmission</subject><ispartof>Current protocols, 2021-12, Vol.1 (12), p.e314-n/a</ispartof><rights>2021 Wiley Periodicals LLC</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3324-4234fa65b9ca5559986046bd6237a1aaabc4370d6eefb88c51a433da86271b153</citedby><cites>FETCH-LOGICAL-c3324-4234fa65b9ca5559986046bd6237a1aaabc4370d6eefb88c51a433da86271b153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcpz1.314$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcpz1.314$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids></links><search><creatorcontrib>Daberkow, David P.</creatorcontrib><creatorcontrib>Hoffman, Kristina</creatorcontrib><creatorcontrib>Kohl, Hannah M.</creatorcontrib><creatorcontrib>Long, Tyrel</creatorcontrib><creatorcontrib>Kirby, Trevor O.</creatorcontrib><creatorcontrib>Ochoa‐Repáraz, Javier</creatorcontrib><title>Microbiome Methods in Experimental Autoimmune Encephalomyelitis</title><title>Current protocols</title><description>Microbiome composition studies are increasingly shedding light on animal models of disease. This paper describes a protocol for analyzing the gut microbiome composition prior to and after the induction of mice to experimental autoimmune encephalomyelitis (EAE), the principal animal model of the human neuroinflammatory demyelinating disease multiple sclerosis (MS). We also address and provide data assessing the impact of mice reared in different animal facilities on EAE induction. Furthermore, we discuss potential regulators of the gut‐microbiome‐brain axis (GMBA) in relation to neuroinflammation and implications on demyelinating disease states. Our results suggest that mice reared in different animal facilities produce different levels of EAE induction. These results highlight the importance of accounting for consistent environmental conditions when inducing EAE and other animal models of disease. © 2021 Wiley Periodicals LLC.
Basic Protocol 1: Study of the composition of the gut microbiome in the neuroinflammatory model of experimental autoimmune encephalomyelitis
Basic Protocol 2: Experimental procedures for DNA extraction and microbiome analysis</description><subject>EAE</subject><subject>experimental autoimmune encephalomyelitis</subject><subject>GMBA</subject><subject>gut‐microbiome‐brain axis</subject><subject>neuroinflammation</subject><subject>neurotransmission</subject><issn>2691-1299</issn><issn>2691-1299</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLw0AURgdRsNSCPyFLN6nzTmYlpdQHtOhCN26GyeSGjkwyMZOg9debUEE3ru5dHD44B6FLgpcEY3pt2y-yZISfoBmViqSEKnX65z9Hixjf8IgKMmJ0hm52znahcKGGZAf9PpQxcU2y-WyhczU0vfHJauiDq-uhgWTTWGj3xof6AN71Ll6gs8r4CIufO0cvt5vn9X26fbx7WK-2qWWM8pRTxisjRaGsEUIolUvMZVFKyjJDjDGF5SzDpQSoijy3ghjOWGlySTNSEMHm6Oq423bhfYDY69pFC96bBsIQNZU4Y1iRjP6io1iMHVS6HVVMd9AE6ymTnjLp0X9E0yP64Twc_uX0-ul16sW-AQ97aFg</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Daberkow, David P.</creator><creator>Hoffman, Kristina</creator><creator>Kohl, Hannah M.</creator><creator>Long, Tyrel</creator><creator>Kirby, Trevor O.</creator><creator>Ochoa‐Repáraz, Javier</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202112</creationdate><title>Microbiome Methods in Experimental Autoimmune Encephalomyelitis</title><author>Daberkow, David P. ; Hoffman, Kristina ; Kohl, Hannah M. ; Long, Tyrel ; Kirby, Trevor O. ; Ochoa‐Repáraz, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3324-4234fa65b9ca5559986046bd6237a1aaabc4370d6eefb88c51a433da86271b153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>EAE</topic><topic>experimental autoimmune encephalomyelitis</topic><topic>GMBA</topic><topic>gut‐microbiome‐brain axis</topic><topic>neuroinflammation</topic><topic>neurotransmission</topic><toplevel>online_resources</toplevel><creatorcontrib>Daberkow, David P.</creatorcontrib><creatorcontrib>Hoffman, Kristina</creatorcontrib><creatorcontrib>Kohl, Hannah M.</creatorcontrib><creatorcontrib>Long, Tyrel</creatorcontrib><creatorcontrib>Kirby, Trevor O.</creatorcontrib><creatorcontrib>Ochoa‐Repáraz, Javier</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daberkow, David P.</au><au>Hoffman, Kristina</au><au>Kohl, Hannah M.</au><au>Long, Tyrel</au><au>Kirby, Trevor O.</au><au>Ochoa‐Repáraz, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbiome Methods in Experimental Autoimmune Encephalomyelitis</atitle><jtitle>Current protocols</jtitle><date>2021-12</date><risdate>2021</risdate><volume>1</volume><issue>12</issue><spage>e314</spage><epage>n/a</epage><pages>e314-n/a</pages><issn>2691-1299</issn><eissn>2691-1299</eissn><abstract>Microbiome composition studies are increasingly shedding light on animal models of disease. This paper describes a protocol for analyzing the gut microbiome composition prior to and after the induction of mice to experimental autoimmune encephalomyelitis (EAE), the principal animal model of the human neuroinflammatory demyelinating disease multiple sclerosis (MS). We also address and provide data assessing the impact of mice reared in different animal facilities on EAE induction. Furthermore, we discuss potential regulators of the gut‐microbiome‐brain axis (GMBA) in relation to neuroinflammation and implications on demyelinating disease states. Our results suggest that mice reared in different animal facilities produce different levels of EAE induction. These results highlight the importance of accounting for consistent environmental conditions when inducing EAE and other animal models of disease. © 2021 Wiley Periodicals LLC.
Basic Protocol 1: Study of the composition of the gut microbiome in the neuroinflammatory model of experimental autoimmune encephalomyelitis
Basic Protocol 2: Experimental procedures for DNA extraction and microbiome analysis</abstract><doi>10.1002/cpz1.314</doi><tpages>23</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Wiley Online Library Journals |
| subjects | EAE experimental autoimmune encephalomyelitis GMBA gut‐microbiome‐brain axis neuroinflammation neurotransmission |
| title | Microbiome Methods in Experimental Autoimmune Encephalomyelitis |
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