REV-ERBs negatively regulate mineralization of the cementoblasts
The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge. Expression pattern of REV-ERBα in cementoblasts was investigated in vivo...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2022-01, Vol.587, p.9-15 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 15 |
|---|---|
| container_issue | |
| container_start_page | 9 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 587 |
| creator | Fu, Liangliang Wang, Min Zhu, Guixin Zhao, Zifan Sun, Huifang Cao, Zhengguo Xia, Haibin |
| description | The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge.
Expression pattern of REV-ERBα in cementoblasts was investigated in vivo and in vitro. CCK-8 assay, scratch wound healing assay, alkaline phosphatase (ALP) and alizarin red S (ARS) staining were performed to evaluate the effects of REV-ERBs activation by SR9009 on proliferation, migration and mineralization of OCCM-30, an immortalized cementoblast cell line. Furthermore, mineralization related markers including osterix (OSX), ALP, bone sialoprotein (BSP) and osteocalcin (OCN) were evaluated.
Strong expression of REV-ERBα was found in cellular cementum around tooth apex. Rev-erbα mRNA oscillated periodically in OCCM-30 and declined after mineralization induction. REV-ERBs activation by SR9009 inhibited proliferation but promoted migration of OCCM-30 in vitro. Results of ALP and ARS staining suggested that REV-ERBs activation negatively regulated mineralization of OCCM-30. Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN.
REV-ERBs are involved in cementogenesis and negatively regulate mineralization of cementoblasts via inhibiting OSX expression. Our study provides a potential target regarding periodontal and cementum regeneration.
[Display omitted]
•Strong expression of REV-ERBα is found during cellular cementum formation.•Rev-erbα in cementoblast oscillates periodically in vitro while declines after mineralization induction.•REV-ERBs activation inhibits proliferation but promotes migration of cementoblast.•REV-ERBs activation attenuates mineralization of cementoblast via downregulating OSX, ALP, BSP and OCN. |
| doi_str_mv | 10.1016/j.bbrc.2021.11.051 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2606924982</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X2101562X</els_id><sourcerecordid>2606924982</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-b63c4e5c00dcab8d979783260dcbb24a6a47b99e86af8331e59695493d3e120d3</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWj_-gAfZo5ddM0k23YAHtdQPKAhFxVtIslNN2Q9Ndgv117ul6tHTMMzzvjAPIadAM6AgL5aZtcFljDLIADKaww4ZAVU0ZUDFLhlRSmXKFLwekMMYl5QCCKn2yQEXhQQxZiNyNZ--pNP5TUwafDOdX2G1TgK-9ZXpMKl9g8FU_mu4tE3SLpLuHROHNTZdaysTu3hM9haminjyM4_I8-30aXKfzh7vHibXs9TxXHapldwJzB2lpTO2KNVYjQvO5LBay4SRRoytUlhIsyg4B8yVVLlQvOQIjJb8iJxvez9C-9lj7HTto8OqMg22fdRDlVRMqIINKNuiLrQxBlzoj-BrE9YaqN6Y00u9Mac35jSAHswNobOf_t7WWP5FflUNwOUWwOHLlcego_PYOCx9QNfpsvX_9X8D3hF-eg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2606924982</pqid></control><display><type>article</type><title>REV-ERBs negatively regulate mineralization of the cementoblasts</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Fu, Liangliang ; Wang, Min ; Zhu, Guixin ; Zhao, Zifan ; Sun, Huifang ; Cao, Zhengguo ; Xia, Haibin</creator><creatorcontrib>Fu, Liangliang ; Wang, Min ; Zhu, Guixin ; Zhao, Zifan ; Sun, Huifang ; Cao, Zhengguo ; Xia, Haibin</creatorcontrib><description>The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge.
Expression pattern of REV-ERBα in cementoblasts was investigated in vivo and in vitro. CCK-8 assay, scratch wound healing assay, alkaline phosphatase (ALP) and alizarin red S (ARS) staining were performed to evaluate the effects of REV-ERBs activation by SR9009 on proliferation, migration and mineralization of OCCM-30, an immortalized cementoblast cell line. Furthermore, mineralization related markers including osterix (OSX), ALP, bone sialoprotein (BSP) and osteocalcin (OCN) were evaluated.
Strong expression of REV-ERBα was found in cellular cementum around tooth apex. Rev-erbα mRNA oscillated periodically in OCCM-30 and declined after mineralization induction. REV-ERBs activation by SR9009 inhibited proliferation but promoted migration of OCCM-30 in vitro. Results of ALP and ARS staining suggested that REV-ERBs activation negatively regulated mineralization of OCCM-30. Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN.
REV-ERBs are involved in cementogenesis and negatively regulate mineralization of cementoblasts via inhibiting OSX expression. Our study provides a potential target regarding periodontal and cementum regeneration.
[Display omitted]
•Strong expression of REV-ERBα is found during cellular cementum formation.•Rev-erbα in cementoblast oscillates periodically in vitro while declines after mineralization induction.•REV-ERBs activation inhibits proliferation but promotes migration of cementoblast.•REV-ERBs activation attenuates mineralization of cementoblast via downregulating OSX, ALP, BSP and OCN.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2021.11.051</identifier><identifier>PMID: 34861472</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; Animals ; Biological Clocks - genetics ; Calcification, Physiologic - genetics ; Cell Differentiation - drug effects ; Cell Line, Transformed ; Cell Proliferation - drug effects ; Cementoblasts ; Cementogenesis - drug effects ; Cementogenesis - genetics ; Dental Cementum - cytology ; Dental Cementum - drug effects ; Dental Cementum - metabolism ; Female ; Gene Expression Regulation ; Humans ; Integrin-Binding Sialoprotein - genetics ; Integrin-Binding Sialoprotein - metabolism ; Mice ; Mice, Inbred C57BL ; Mineralization ; Nuclear Receptor Subfamily 1, Group D, Member 1 - genetics ; Nuclear Receptor Subfamily 1, Group D, Member 1 - metabolism ; Osteocalcin - genetics ; Osteocalcin - metabolism ; Pyrrolidines - pharmacology ; Regeneration ; REV-ERBs ; Signal Transduction ; Sp7 Transcription Factor - genetics ; Sp7 Transcription Factor - metabolism ; SR9009 ; Thiophenes - pharmacology</subject><ispartof>Biochemical and biophysical research communications, 2022-01, Vol.587, p.9-15</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-b63c4e5c00dcab8d979783260dcbb24a6a47b99e86af8331e59695493d3e120d3</citedby><cites>FETCH-LOGICAL-c356t-b63c4e5c00dcab8d979783260dcbb24a6a47b99e86af8331e59695493d3e120d3</cites><orcidid>0000-0003-0085-6995 ; 0000-0003-2550-1146 ; 0000-0001-9619-9437</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X2101562X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34861472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Liangliang</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Zhu, Guixin</creatorcontrib><creatorcontrib>Zhao, Zifan</creatorcontrib><creatorcontrib>Sun, Huifang</creatorcontrib><creatorcontrib>Cao, Zhengguo</creatorcontrib><creatorcontrib>Xia, Haibin</creatorcontrib><title>REV-ERBs negatively regulate mineralization of the cementoblasts</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge.
Expression pattern of REV-ERBα in cementoblasts was investigated in vivo and in vitro. CCK-8 assay, scratch wound healing assay, alkaline phosphatase (ALP) and alizarin red S (ARS) staining were performed to evaluate the effects of REV-ERBs activation by SR9009 on proliferation, migration and mineralization of OCCM-30, an immortalized cementoblast cell line. Furthermore, mineralization related markers including osterix (OSX), ALP, bone sialoprotein (BSP) and osteocalcin (OCN) were evaluated.
Strong expression of REV-ERBα was found in cellular cementum around tooth apex. Rev-erbα mRNA oscillated periodically in OCCM-30 and declined after mineralization induction. REV-ERBs activation by SR9009 inhibited proliferation but promoted migration of OCCM-30 in vitro. Results of ALP and ARS staining suggested that REV-ERBs activation negatively regulated mineralization of OCCM-30. Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN.
REV-ERBs are involved in cementogenesis and negatively regulate mineralization of cementoblasts via inhibiting OSX expression. Our study provides a potential target regarding periodontal and cementum regeneration.
[Display omitted]
•Strong expression of REV-ERBα is found during cellular cementum formation.•Rev-erbα in cementoblast oscillates periodically in vitro while declines after mineralization induction.•REV-ERBs activation inhibits proliferation but promotes migration of cementoblast.•REV-ERBs activation attenuates mineralization of cementoblast via downregulating OSX, ALP, BSP and OCN.</description><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biological Clocks - genetics</subject><subject>Calcification, Physiologic - genetics</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line, Transformed</subject><subject>Cell Proliferation - drug effects</subject><subject>Cementoblasts</subject><subject>Cementogenesis - drug effects</subject><subject>Cementogenesis - genetics</subject><subject>Dental Cementum - cytology</subject><subject>Dental Cementum - drug effects</subject><subject>Dental Cementum - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Integrin-Binding Sialoprotein - genetics</subject><subject>Integrin-Binding Sialoprotein - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mineralization</subject><subject>Nuclear Receptor Subfamily 1, Group D, Member 1 - genetics</subject><subject>Nuclear Receptor Subfamily 1, Group D, Member 1 - metabolism</subject><subject>Osteocalcin - genetics</subject><subject>Osteocalcin - metabolism</subject><subject>Pyrrolidines - pharmacology</subject><subject>Regeneration</subject><subject>REV-ERBs</subject><subject>Signal Transduction</subject><subject>Sp7 Transcription Factor - genetics</subject><subject>Sp7 Transcription Factor - metabolism</subject><subject>SR9009</subject><subject>Thiophenes - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoWj_-gAfZo5ddM0k23YAHtdQPKAhFxVtIslNN2Q9Ndgv117ul6tHTMMzzvjAPIadAM6AgL5aZtcFljDLIADKaww4ZAVU0ZUDFLhlRSmXKFLwekMMYl5QCCKn2yQEXhQQxZiNyNZ--pNP5TUwafDOdX2G1TgK-9ZXpMKl9g8FU_mu4tE3SLpLuHROHNTZdaysTu3hM9haminjyM4_I8-30aXKfzh7vHibXs9TxXHapldwJzB2lpTO2KNVYjQvO5LBay4SRRoytUlhIsyg4B8yVVLlQvOQIjJb8iJxvez9C-9lj7HTto8OqMg22fdRDlVRMqIINKNuiLrQxBlzoj-BrE9YaqN6Y00u9Mac35jSAHswNobOf_t7WWP5FflUNwOUWwOHLlcego_PYOCx9QNfpsvX_9X8D3hF-eg</recordid><startdate>20220108</startdate><enddate>20220108</enddate><creator>Fu, Liangliang</creator><creator>Wang, Min</creator><creator>Zhu, Guixin</creator><creator>Zhao, Zifan</creator><creator>Sun, Huifang</creator><creator>Cao, Zhengguo</creator><creator>Xia, Haibin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0085-6995</orcidid><orcidid>https://orcid.org/0000-0003-2550-1146</orcidid><orcidid>https://orcid.org/0000-0001-9619-9437</orcidid></search><sort><creationdate>20220108</creationdate><title>REV-ERBs negatively regulate mineralization of the cementoblasts</title><author>Fu, Liangliang ; Wang, Min ; Zhu, Guixin ; Zhao, Zifan ; Sun, Huifang ; Cao, Zhengguo ; Xia, Haibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-b63c4e5c00dcab8d979783260dcbb24a6a47b99e86af8331e59695493d3e120d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biological Clocks - genetics</topic><topic>Calcification, Physiologic - genetics</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line, Transformed</topic><topic>Cell Proliferation - drug effects</topic><topic>Cementoblasts</topic><topic>Cementogenesis - drug effects</topic><topic>Cementogenesis - genetics</topic><topic>Dental Cementum - cytology</topic><topic>Dental Cementum - drug effects</topic><topic>Dental Cementum - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Integrin-Binding Sialoprotein - genetics</topic><topic>Integrin-Binding Sialoprotein - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mineralization</topic><topic>Nuclear Receptor Subfamily 1, Group D, Member 1 - genetics</topic><topic>Nuclear Receptor Subfamily 1, Group D, Member 1 - metabolism</topic><topic>Osteocalcin - genetics</topic><topic>Osteocalcin - metabolism</topic><topic>Pyrrolidines - pharmacology</topic><topic>Regeneration</topic><topic>REV-ERBs</topic><topic>Signal Transduction</topic><topic>Sp7 Transcription Factor - genetics</topic><topic>Sp7 Transcription Factor - metabolism</topic><topic>SR9009</topic><topic>Thiophenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Liangliang</creatorcontrib><creatorcontrib>Wang, Min</creatorcontrib><creatorcontrib>Zhu, Guixin</creatorcontrib><creatorcontrib>Zhao, Zifan</creatorcontrib><creatorcontrib>Sun, Huifang</creatorcontrib><creatorcontrib>Cao, Zhengguo</creatorcontrib><creatorcontrib>Xia, Haibin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Liangliang</au><au>Wang, Min</au><au>Zhu, Guixin</au><au>Zhao, Zifan</au><au>Sun, Huifang</au><au>Cao, Zhengguo</au><au>Xia, Haibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>REV-ERBs negatively regulate mineralization of the cementoblasts</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2022-01-08</date><risdate>2022</risdate><volume>587</volume><spage>9</spage><epage>15</epage><pages>9-15</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The role of circadian clock in cementogenesis is unclear. This study examines the role of REV-ERBs, one of circadian clock proteins, in proliferation, migration and mineralization of cementoblasts to fill the gap in knowledge.
Expression pattern of REV-ERBα in cementoblasts was investigated in vivo and in vitro. CCK-8 assay, scratch wound healing assay, alkaline phosphatase (ALP) and alizarin red S (ARS) staining were performed to evaluate the effects of REV-ERBs activation by SR9009 on proliferation, migration and mineralization of OCCM-30, an immortalized cementoblast cell line. Furthermore, mineralization related markers including osterix (OSX), ALP, bone sialoprotein (BSP) and osteocalcin (OCN) were evaluated.
Strong expression of REV-ERBα was found in cellular cementum around tooth apex. Rev-erbα mRNA oscillated periodically in OCCM-30 and declined after mineralization induction. REV-ERBs activation by SR9009 inhibited proliferation but promoted migration of OCCM-30 in vitro. Results of ALP and ARS staining suggested that REV-ERBs activation negatively regulated mineralization of OCCM-30. Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN.
REV-ERBs are involved in cementogenesis and negatively regulate mineralization of cementoblasts via inhibiting OSX expression. Our study provides a potential target regarding periodontal and cementum regeneration.
[Display omitted]
•Strong expression of REV-ERBα is found during cellular cementum formation.•Rev-erbα in cementoblast oscillates periodically in vitro while declines after mineralization induction.•REV-ERBs activation inhibits proliferation but promotes migration of cementoblast.•REV-ERBs activation attenuates mineralization of cementoblast via downregulating OSX, ALP, BSP and OCN.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34861472</pmid><doi>10.1016/j.bbrc.2021.11.051</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0085-6995</orcidid><orcidid>https://orcid.org/0000-0003-2550-1146</orcidid><orcidid>https://orcid.org/0000-0001-9619-9437</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2022-01, Vol.587, p.9-15 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2606924982 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Animals Biological Clocks - genetics Calcification, Physiologic - genetics Cell Differentiation - drug effects Cell Line, Transformed Cell Proliferation - drug effects Cementoblasts Cementogenesis - drug effects Cementogenesis - genetics Dental Cementum - cytology Dental Cementum - drug effects Dental Cementum - metabolism Female Gene Expression Regulation Humans Integrin-Binding Sialoprotein - genetics Integrin-Binding Sialoprotein - metabolism Mice Mice, Inbred C57BL Mineralization Nuclear Receptor Subfamily 1, Group D, Member 1 - genetics Nuclear Receptor Subfamily 1, Group D, Member 1 - metabolism Osteocalcin - genetics Osteocalcin - metabolism Pyrrolidines - pharmacology Regeneration REV-ERBs Signal Transduction Sp7 Transcription Factor - genetics Sp7 Transcription Factor - metabolism SR9009 Thiophenes - pharmacology |
| title | REV-ERBs negatively regulate mineralization of the cementoblasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T10%3A14%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=REV-ERBs%20negatively%20regulate%20mineralization%20of%20the%20cementoblasts&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Fu,%20Liangliang&rft.date=2022-01-08&rft.volume=587&rft.spage=9&rft.epage=15&rft.pages=9-15&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2021.11.051&rft_dat=%3Cproquest_cross%3E2606924982%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2606924982&rft_id=info:pmid/34861472&rft_els_id=S0006291X2101562X&rfr_iscdi=true |