Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis
Background Dendrobium is a genus of plants used as traditional Chinese herbal medicines, with high economic and medicinal value. Methods and results To reveal the mechanism of flavonoid biosynthesis in Dendrobium , the metabolites and transcriptomes of four Dendrobium species ( D. chrysotoxum , D. n...
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| description | Background
Dendrobium
is a genus of plants used as traditional Chinese herbal medicines, with high economic and medicinal value.
Methods and results
To reveal the mechanism of flavonoid biosynthesis in
Dendrobium
, the metabolites and transcriptomes of four
Dendrobium
species (
D. chrysotoxum
,
D. nobile
,
D. fimbriatum
, and
D. denneanum
) were analyzed comprehensively. Ultra-high-performance liquid chromatography-tandem mass spectrometry analysis revealed ten flavonoid compounds in
Dendrobium
. In total, 100,096 unigenes were obtained from the transcript database of the four
Dendrobium
species. Among the identified differentially expressed genes, 51 were associated with flavonoid biosynthesis, and 670 differentially expressed transcription factors were predicted, including 194 MYB, 87 bHLH, and 100 WRKY family transcription factors, respectively. Transcriptome analysis showed that the expression levels of structural genes such as
chalcone synthase
(
CHS
),
cinnamate-4-hydroxylase
(
C4H
), and
flavonoid 3′-hydroxylase
(
F3′H
) were lower in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
than those in
D. denneanum
, which may be the main reason for the low flavonoid contents in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
.
Conclusions
The expression level of structural genes corresponded to the accumulation level of flavonols in the different
Dendrobium
species. The results deepen the understanding of the molecular mechanism of flavonoid biosynthesis in
Dendrobium
and provide novel insights into the synthesis and accumulation of flavonoids in
Dendrobium
. |
| doi_str_mv | 10.1007/s11033-021-07023-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2605233345</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2605233345</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-d6b245c5bc8b26e363ac4787820865a4ffe32e538ae252e426271111e89b073a3</originalsourceid><addsrcrecordid>eNp9kcGOFCEQhonRuOPqC3gwJF68tBYUNL1Hs-uqySZe9EyArl5Zu2GEbpN5exln1cSDdSFVfPVXwc_YcwGvBYB5U4UAxA6k6MCAxA4fsJ3QBjt1YYaHbAcIolODFmfsSa13AKCE0Y_ZGR6LaoAd-3Y9ux855ThyH3M9pPUr1Vh5THzKW-FXlMaSfdwWXvcUIlXuXaWR58TX4lINJe7XvBCv9H2jFGK65S6NfKHV-TzHlVrq5kMTfcoeTW6u9Oz-PGdfrt99vvzQ3Xx6__Hy7U0X0Oi1G3svlQ7ah8HLnrBHF5QZzCBh6LVT00QoSePgSGpJSvbSiBY0XHgw6PCcvTrp7ktuO9XVLrEGmmeXKG_Vyh60RESlG_ryH_Suvbrte6RQKKMNQKPkiQol11posvsSF1cOVoA9WmFPVthmhf1lhcXW9OJeevMLjX9afv99A_AE1HaVbqn8nf0f2Z_UxpRk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2631475700</pqid></control><display><type>article</type><title>Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Wang, Yajuan ; Jia, Ning ; Wang, Peiyu ; Liu, Jiameng ; Sun, Jing ; Ye, Wei ; Fan, Bei</creator><creatorcontrib>Wang, Yajuan ; Jia, Ning ; Wang, Peiyu ; Liu, Jiameng ; Sun, Jing ; Ye, Wei ; Fan, Bei</creatorcontrib><description>Background
Dendrobium
is a genus of plants used as traditional Chinese herbal medicines, with high economic and medicinal value.
Methods and results
To reveal the mechanism of flavonoid biosynthesis in
Dendrobium
, the metabolites and transcriptomes of four
Dendrobium
species (
D. chrysotoxum
,
D. nobile
,
D. fimbriatum
, and
D. denneanum
) were analyzed comprehensively. Ultra-high-performance liquid chromatography-tandem mass spectrometry analysis revealed ten flavonoid compounds in
Dendrobium
. In total, 100,096 unigenes were obtained from the transcript database of the four
Dendrobium
species. Among the identified differentially expressed genes, 51 were associated with flavonoid biosynthesis, and 670 differentially expressed transcription factors were predicted, including 194 MYB, 87 bHLH, and 100 WRKY family transcription factors, respectively. Transcriptome analysis showed that the expression levels of structural genes such as
chalcone synthase
(
CHS
),
cinnamate-4-hydroxylase
(
C4H
), and
flavonoid 3′-hydroxylase
(
F3′H
) were lower in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
than those in
D. denneanum
, which may be the main reason for the low flavonoid contents in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
.
Conclusions
The expression level of structural genes corresponded to the accumulation level of flavonols in the different
Dendrobium
species. The results deepen the understanding of the molecular mechanism of flavonoid biosynthesis in
Dendrobium
and provide novel insights into the synthesis and accumulation of flavonoids in
Dendrobium
.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-021-07023-3</identifier><identifier>PMID: 34851480</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Biosynthesis ; Chalcone synthase ; Cinnamate 4-hydroxylase ; Dendrobium ; Dendrobium - genetics ; Dendrobium - metabolism ; Flavonoids ; Flavonoids - metabolism ; Flavonols ; Gene Expression Profiling ; Gene Expression Regulation, Plant - genetics ; Herbal medicine ; High-performance liquid chromatography ; Histology ; Life Sciences ; Mass spectroscopy ; Medicinal plants ; Morphology ; Original Article ; Species ; Transcription factors ; Transcription Factors - genetics ; Transcriptome - genetics ; Transcriptomes</subject><ispartof>Molecular biology reports, 2022-03, Vol.49 (3), p.2047-2057</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-d6b245c5bc8b26e363ac4787820865a4ffe32e538ae252e426271111e89b073a3</citedby><cites>FETCH-LOGICAL-c375t-d6b245c5bc8b26e363ac4787820865a4ffe32e538ae252e426271111e89b073a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-021-07023-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-021-07023-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34851480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yajuan</creatorcontrib><creatorcontrib>Jia, Ning</creatorcontrib><creatorcontrib>Wang, Peiyu</creatorcontrib><creatorcontrib>Liu, Jiameng</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Ye, Wei</creatorcontrib><creatorcontrib>Fan, Bei</creatorcontrib><title>Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Dendrobium
is a genus of plants used as traditional Chinese herbal medicines, with high economic and medicinal value.
Methods and results
To reveal the mechanism of flavonoid biosynthesis in
Dendrobium
, the metabolites and transcriptomes of four
Dendrobium
species (
D. chrysotoxum
,
D. nobile
,
D. fimbriatum
, and
D. denneanum
) were analyzed comprehensively. Ultra-high-performance liquid chromatography-tandem mass spectrometry analysis revealed ten flavonoid compounds in
Dendrobium
. In total, 100,096 unigenes were obtained from the transcript database of the four
Dendrobium
species. Among the identified differentially expressed genes, 51 were associated with flavonoid biosynthesis, and 670 differentially expressed transcription factors were predicted, including 194 MYB, 87 bHLH, and 100 WRKY family transcription factors, respectively. Transcriptome analysis showed that the expression levels of structural genes such as
chalcone synthase
(
CHS
),
cinnamate-4-hydroxylase
(
C4H
), and
flavonoid 3′-hydroxylase
(
F3′H
) were lower in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
than those in
D. denneanum
, which may be the main reason for the low flavonoid contents in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
.
Conclusions
The expression level of structural genes corresponded to the accumulation level of flavonols in the different
Dendrobium
species. The results deepen the understanding of the molecular mechanism of flavonoid biosynthesis in
Dendrobium
and provide novel insights into the synthesis and accumulation of flavonoids in
Dendrobium
.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Chalcone synthase</subject><subject>Cinnamate 4-hydroxylase</subject><subject>Dendrobium</subject><subject>Dendrobium - genetics</subject><subject>Dendrobium - metabolism</subject><subject>Flavonoids</subject><subject>Flavonoids - metabolism</subject><subject>Flavonols</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Herbal medicine</subject><subject>High-performance liquid chromatography</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Mass spectroscopy</subject><subject>Medicinal plants</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Species</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcriptome - genetics</subject><subject>Transcriptomes</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcGOFCEQhonRuOPqC3gwJF68tBYUNL1Hs-uqySZe9EyArl5Zu2GEbpN5exln1cSDdSFVfPVXwc_YcwGvBYB5U4UAxA6k6MCAxA4fsJ3QBjt1YYaHbAcIolODFmfsSa13AKCE0Y_ZGR6LaoAd-3Y9ux855ThyH3M9pPUr1Vh5THzKW-FXlMaSfdwWXvcUIlXuXaWR58TX4lINJe7XvBCv9H2jFGK65S6NfKHV-TzHlVrq5kMTfcoeTW6u9Oz-PGdfrt99vvzQ3Xx6__Hy7U0X0Oi1G3svlQ7ah8HLnrBHF5QZzCBh6LVT00QoSePgSGpJSvbSiBY0XHgw6PCcvTrp7ktuO9XVLrEGmmeXKG_Vyh60RESlG_ryH_Suvbrte6RQKKMNQKPkiQol11posvsSF1cOVoA9WmFPVthmhf1lhcXW9OJeevMLjX9afv99A_AE1HaVbqn8nf0f2Z_UxpRk</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Wang, Yajuan</creator><creator>Jia, Ning</creator><creator>Wang, Peiyu</creator><creator>Liu, Jiameng</creator><creator>Sun, Jing</creator><creator>Ye, Wei</creator><creator>Fan, Bei</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20220301</creationdate><title>Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis</title><author>Wang, Yajuan ; Jia, Ning ; Wang, Peiyu ; Liu, Jiameng ; Sun, Jing ; Ye, Wei ; Fan, Bei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-d6b245c5bc8b26e363ac4787820865a4ffe32e538ae252e426271111e89b073a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Chalcone synthase</topic><topic>Cinnamate 4-hydroxylase</topic><topic>Dendrobium</topic><topic>Dendrobium - genetics</topic><topic>Dendrobium - metabolism</topic><topic>Flavonoids</topic><topic>Flavonoids - metabolism</topic><topic>Flavonols</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Herbal medicine</topic><topic>High-performance liquid chromatography</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Mass spectroscopy</topic><topic>Medicinal plants</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Species</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcriptome - genetics</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yajuan</creatorcontrib><creatorcontrib>Jia, Ning</creatorcontrib><creatorcontrib>Wang, Peiyu</creatorcontrib><creatorcontrib>Liu, Jiameng</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Ye, Wei</creatorcontrib><creatorcontrib>Fan, Bei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yajuan</au><au>Jia, Ning</au><au>Wang, Peiyu</au><au>Liu, Jiameng</au><au>Sun, Jing</au><au>Ye, Wei</au><au>Fan, Bei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>49</volume><issue>3</issue><spage>2047</spage><epage>2057</epage><pages>2047-2057</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Dendrobium
is a genus of plants used as traditional Chinese herbal medicines, with high economic and medicinal value.
Methods and results
To reveal the mechanism of flavonoid biosynthesis in
Dendrobium
, the metabolites and transcriptomes of four
Dendrobium
species (
D. chrysotoxum
,
D. nobile
,
D. fimbriatum
, and
D. denneanum
) were analyzed comprehensively. Ultra-high-performance liquid chromatography-tandem mass spectrometry analysis revealed ten flavonoid compounds in
Dendrobium
. In total, 100,096 unigenes were obtained from the transcript database of the four
Dendrobium
species. Among the identified differentially expressed genes, 51 were associated with flavonoid biosynthesis, and 670 differentially expressed transcription factors were predicted, including 194 MYB, 87 bHLH, and 100 WRKY family transcription factors, respectively. Transcriptome analysis showed that the expression levels of structural genes such as
chalcone synthase
(
CHS
),
cinnamate-4-hydroxylase
(
C4H
), and
flavonoid 3′-hydroxylase
(
F3′H
) were lower in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
than those in
D. denneanum
, which may be the main reason for the low flavonoid contents in
D. chrysotoxum
,
D. nobile
, and
D. fimbriatum
.
Conclusions
The expression level of structural genes corresponded to the accumulation level of flavonols in the different
Dendrobium
species. The results deepen the understanding of the molecular mechanism of flavonoid biosynthesis in
Dendrobium
and provide novel insights into the synthesis and accumulation of flavonoids in
Dendrobium
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34851480</pmid><doi>10.1007/s11033-021-07023-3</doi><tpages>11</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Biosynthesis Chalcone synthase Cinnamate 4-hydroxylase Dendrobium Dendrobium - genetics Dendrobium - metabolism Flavonoids Flavonoids - metabolism Flavonols Gene Expression Profiling Gene Expression Regulation, Plant - genetics Herbal medicine High-performance liquid chromatography Histology Life Sciences Mass spectroscopy Medicinal plants Morphology Original Article Species Transcription factors Transcription Factors - genetics Transcriptome - genetics Transcriptomes |
| title | Flavonoid biosynthesis in four Dendrobium species based on transcriptome sequencing and metabolite analysis |
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