High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system

The detection of antibody to non-structural protein (NSP) of Foot-and-mouth disease virus (FMDV) is the reliable diagnostic method for differentiating infected from vaccinated animals (DIVA). For this purpose, the detection of antibodies to non-structural 3ABC protein is suitable for identification...

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Veröffentlicht in:	Protein expression and purification 2022-03, Vol.191, p.106025-106025, Article 106025
Hauptverfasser:	Zia, Muhammad Ashir, Shah, Muhammad Salahuddin, Shafqat Ali Khan, Rai, Farooq, Umer, Shafi, Jamila, Habib, Mudasser
Format:	Artikel
Sprache:	eng
Schlagworte:	ELISA Foot-and-mouth disease virus (FMDV) Foot-and-Mouth Disease Virus - chemistry Foot-and-Mouth Disease Virus - genetics Gene Expression Ni-NTA chromatography Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification SUMO (small ubiquitin-like modifiers) SUMO-1 Protein - biosynthesis SUMO-1 Protein - chemistry SUMO-1 Protein - genetics SUMO-1 Protein - isolation & purification Viral Nonstructural Proteins - biosynthesis Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - isolation & purification
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