Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize
SUMMARY The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map‐based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2022-02, Vol.109 (4), p.980-991 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Wang, Yanli He, Shijiang Long, Yun Zhang, Xiaoling Zhang, Xiaoxiang Hu, Hongmei Li, Zhaoling Hou, Fengxia Ge, Fei Gao, Shibin Pan, Guangtang Ma, Langlang Shen, Yaou |
| description | SUMMARY
The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map‐based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities for ECs. We demonstrated that ZmSAUR15, which encodes a small auxin‐upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole‐3‐acetic acid biosynthesis and cell division in immature embryo‐derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
Significance Statement
Embryonic callus formation is highly genotype dependent in maize, which largely limits maize transgenic breeding development. This study found that ZmSAUR15 negatively effects embryonic callus formation in maize. The polymorphisms in the ZmSAUR15 promoter influence its expression. Furthermore, ZmSAUR15 modulates indole‐3‐acetic acid biosynthesis and cell division in callus. |
| doi_str_mv | 10.1111/tpj.15609 |
| format | Article |
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The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map‐based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities for ECs. We demonstrated that ZmSAUR15, which encodes a small auxin‐upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole‐3‐acetic acid biosynthesis and cell division in immature embryo‐derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
Significance Statement
Embryonic callus formation is highly genotype dependent in maize, which largely limits maize transgenic breeding development. This study found that ZmSAUR15 negatively effects embryonic callus formation in maize. The polymorphisms in the ZmSAUR15 promoter influence its expression. Furthermore, ZmSAUR15 modulates indole‐3‐acetic acid biosynthesis and cell division in callus.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/tpj.15609</identifier><identifier>PMID: 34822726</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Acetic acid ; Arabidopsis - genetics ; Arabidopsis Proteins ; association mapping ; Biosynthesis ; Callus ; Cell Division ; Cloning ; Corn ; Embryogenesis ; embryonic callus formation ; Embryos ; functional revelation ; Gene Expression Regulation, Plant ; Genetic diversity ; Genetic transformation ; Genetic Variation ; Inbreeding ; Indoleacetic acid ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; maize ; Phenotype ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Promoter Regions, Genetic ; Regeneration ; Zea mays ; Zea mays - genetics ; Zea mays - metabolism ; ZmSAUR15</subject><ispartof>The Plant journal : for cell and molecular biology, 2022-02, Vol.109 (4), p.980-991</ispartof><rights>2021 Society for Experimental Biology and John Wiley & Sons Ltd</rights><rights>2021 Society for Experimental Biology and John Wiley & Sons Ltd.</rights><rights>Copyright © 2022 John Wiley & Sons Ltd and the Society for Experimental Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4549-2a17c36ef1d52fd3bad365b35a2f892d1a5af769fef4af17b6f8223bd16f90903</citedby><cites>FETCH-LOGICAL-c4549-2a17c36ef1d52fd3bad365b35a2f892d1a5af769fef4af17b6f8223bd16f90903</cites><orcidid>0000-0001-7603-0108</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftpj.15609$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftpj.15609$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34822726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yanli</creatorcontrib><creatorcontrib>He, Shijiang</creatorcontrib><creatorcontrib>Long, Yun</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><creatorcontrib>Zhang, Xiaoxiang</creatorcontrib><creatorcontrib>Hu, Hongmei</creatorcontrib><creatorcontrib>Li, Zhaoling</creatorcontrib><creatorcontrib>Hou, Fengxia</creatorcontrib><creatorcontrib>Ge, Fei</creatorcontrib><creatorcontrib>Gao, Shibin</creatorcontrib><creatorcontrib>Pan, Guangtang</creatorcontrib><creatorcontrib>Ma, Langlang</creatorcontrib><creatorcontrib>Shen, Yaou</creatorcontrib><title>Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>SUMMARY
The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map‐based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities for ECs. We demonstrated that ZmSAUR15, which encodes a small auxin‐upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole‐3‐acetic acid biosynthesis and cell division in immature embryo‐derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
Significance Statement
Embryonic callus formation is highly genotype dependent in maize, which largely limits maize transgenic breeding development. This study found that ZmSAUR15 negatively effects embryonic callus formation in maize. The polymorphisms in the ZmSAUR15 promoter influence its expression. Furthermore, ZmSAUR15 modulates indole‐3‐acetic acid biosynthesis and cell division in callus.</description><subject>Acetic acid</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins</subject><subject>association mapping</subject><subject>Biosynthesis</subject><subject>Callus</subject><subject>Cell Division</subject><subject>Cloning</subject><subject>Corn</subject><subject>Embryogenesis</subject><subject>embryonic callus formation</subject><subject>Embryos</subject><subject>functional revelation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genetic diversity</subject><subject>Genetic transformation</subject><subject>Genetic Variation</subject><subject>Inbreeding</subject><subject>Indoleacetic acid</subject><subject>Intracellular Signaling Peptides and Proteins - genetics</subject><subject>Intracellular Signaling Peptides and Proteins - metabolism</subject><subject>maize</subject><subject>Phenotype</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic</subject><subject>Regeneration</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><subject>Zea mays - metabolism</subject><subject>ZmSAUR15</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctKxDAUhoMoOo4ufAEJuNFFNZc2bZYyeGVA8QLipqTtCWZomzFplXHlI_iMPolxZnQheDY5hI-P_PkR2qHkkIY56qaTQ5oIIlfQgHKRRJzyh1U0IFKQKI0p20Cb3k8IoSkX8Tra4HHGWMrEAPkzaKEzJX5RzqjO2NZj0-LH5vb4_oYmuLRt50zRd4A7i7snwNq6Zg5iq7Fpwt47wNAUbmY_3z8qcOYFquVFG8ylquvew9zbKPMGW2hNq9rD9vIcovvTk7vReTS-OrsYHY-jMk5iGTFF05IL0LRKmK54oaqQreCJYjqTrKIqUToVUoOOlaZpIXRIxYuKCi2JJHyI9hfeqbPPPfgub4wvoa5VC7b3ORMkJoxKmQV07w86sb1rw-sCxUmacZmJQB0sqNJZ7x3ofOpMo9wspyT_biIPTeTzJgK7uzT2RQPVL_nz9QE4WgCvpobZ_6b87vpyofwCVY2UlQ</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Wang, Yanli</creator><creator>He, Shijiang</creator><creator>Long, Yun</creator><creator>Zhang, Xiaoling</creator><creator>Zhang, Xiaoxiang</creator><creator>Hu, Hongmei</creator><creator>Li, Zhaoling</creator><creator>Hou, Fengxia</creator><creator>Ge, Fei</creator><creator>Gao, Shibin</creator><creator>Pan, Guangtang</creator><creator>Ma, Langlang</creator><creator>Shen, Yaou</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7603-0108</orcidid></search><sort><creationdate>202202</creationdate><title>Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize</title><author>Wang, Yanli ; He, Shijiang ; Long, Yun ; Zhang, Xiaoling ; Zhang, Xiaoxiang ; Hu, Hongmei ; Li, Zhaoling ; Hou, Fengxia ; Ge, Fei ; Gao, Shibin ; Pan, Guangtang ; Ma, Langlang ; Shen, Yaou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4549-2a17c36ef1d52fd3bad365b35a2f892d1a5af769fef4af17b6f8223bd16f90903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acetic acid</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins</topic><topic>association mapping</topic><topic>Biosynthesis</topic><topic>Callus</topic><topic>Cell Division</topic><topic>Cloning</topic><topic>Corn</topic><topic>Embryogenesis</topic><topic>embryonic callus formation</topic><topic>Embryos</topic><topic>functional revelation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genetic diversity</topic><topic>Genetic transformation</topic><topic>Genetic Variation</topic><topic>Inbreeding</topic><topic>Indoleacetic acid</topic><topic>Intracellular Signaling Peptides and Proteins - genetics</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>maize</topic><topic>Phenotype</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic</topic><topic>Regeneration</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><topic>Zea mays - metabolism</topic><topic>ZmSAUR15</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yanli</creatorcontrib><creatorcontrib>He, Shijiang</creatorcontrib><creatorcontrib>Long, Yun</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><creatorcontrib>Zhang, Xiaoxiang</creatorcontrib><creatorcontrib>Hu, Hongmei</creatorcontrib><creatorcontrib>Li, Zhaoling</creatorcontrib><creatorcontrib>Hou, Fengxia</creatorcontrib><creatorcontrib>Ge, Fei</creatorcontrib><creatorcontrib>Gao, Shibin</creatorcontrib><creatorcontrib>Pan, Guangtang</creatorcontrib><creatorcontrib>Ma, Langlang</creatorcontrib><creatorcontrib>Shen, Yaou</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yanli</au><au>He, Shijiang</au><au>Long, Yun</au><au>Zhang, Xiaoling</au><au>Zhang, Xiaoxiang</au><au>Hu, Hongmei</au><au>Li, Zhaoling</au><au>Hou, Fengxia</au><au>Ge, Fei</au><au>Gao, Shibin</au><au>Pan, Guangtang</au><au>Ma, Langlang</au><au>Shen, Yaou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2022-02</date><risdate>2022</risdate><volume>109</volume><issue>4</issue><spage>980</spage><epage>991</epage><pages>980-991</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>SUMMARY
The ability of immature maize (Zea mays) embryos to form embryonic calluses (ECs) is highly genotype dependent, which limits transgenic breeding development in maize. Here, we report the association map‐based cloning of ZmSAUR15 using an association panel (AP) consisting of 309 inbred lines with diverse formation abilities for ECs. We demonstrated that ZmSAUR15, which encodes a small auxin‐upregulated RNA, acts as a negative effector in maize EC induction. Polymorphisms in the ZmSAUR15 promoter that influence the expression of ZmSAUR15 transcripts modulate the EC induction capacity in maize. ZmSAUR15 is involved in indole‐3‐acetic acid biosynthesis and cell division in immature embryo‐derived callus. The ability of immature embryos to induce EC formation can be improved by the knockout of ZmSAUR15, which consequently increases the callus regeneration efficiency. Our study provides new insights into overcoming the genotypic limitations associated with EC formation and improving genetic transformation in maize.
Significance Statement
Embryonic callus formation is highly genotype dependent in maize, which largely limits maize transgenic breeding development. This study found that ZmSAUR15 negatively effects embryonic callus formation in maize. The polymorphisms in the ZmSAUR15 promoter influence its expression. Furthermore, ZmSAUR15 modulates indole‐3‐acetic acid biosynthesis and cell division in callus.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>34822726</pmid><doi>10.1111/tpj.15609</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7603-0108</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | The Plant journal : for cell and molecular biology, 2022-02, Vol.109 (4), p.980-991 |
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| language | eng |
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| subjects | Acetic acid Arabidopsis - genetics Arabidopsis Proteins association mapping Biosynthesis Callus Cell Division Cloning Corn Embryogenesis embryonic callus formation Embryos functional revelation Gene Expression Regulation, Plant Genetic diversity Genetic transformation Genetic Variation Inbreeding Indoleacetic acid Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism maize Phenotype Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Promoter Regions, Genetic Regeneration Zea mays Zea mays - genetics Zea mays - metabolism ZmSAUR15 |
| title | Genetic variations in ZmSAUR15 contribute to the formation of immature embryo‐derived embryonic calluses in maize |
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