Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv

Aims This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv. Methods and Results The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gt...

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Veröffentlicht in:	Journal of applied microbiology 2022-04, Vol.132 (4), p.2980-2989
Hauptverfasser:	Li, Sha, Ji, Shunshi, Zhu, Xiong, Chen, Hai, Jin, Dong
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglutination Amplification Antigens Assaying Deoxyribonucleic acid DNA loop‐mediated isothermal amplification1 Molecular Diagnostic Techniques Multiplex Polymerase Chain Reaction - methods Nucleic Acid Amplification Techniques Pathogens Polymerase chain reaction Sensitivity analysis sensitivity4 Serogroup serotype3 Serotypes Serotyping Serotyping - methods Shigella Shigella - genetics Shigella flexneri Shigella flexneri - genetics Shigella flexneri 2 specificity5 Strains (organisms)
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