Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv
Aims This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv. Methods and Results The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gt...
Gespeichert in:
| Veröffentlicht in: | Journal of applied microbiology 2022-04, Vol.132 (4), p.2980-2989 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2989 |
|---|---|
| container_issue | 4 |
| container_start_page | 2980 |
| container_title | Journal of applied microbiology |
| container_volume | 132 |
| creator | Li, Sha Ji, Shunshi Zhu, Xiong Chen, Hai Jin, Dong |
| description | Aims
This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv.
Methods and Results
The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gtrX, opt and wzx was developed. Its specificity and sensitivity were evaluated with 19 serotypes of S. flexneri and 96 other Shigella species and bacterial pathogens commonly found in stool samples. This LAMP assay was completed within 20 min at 61°C and could detect boiled DNA samples at concentrations as low as 1 pg/μl. The S. flexneri serotype LAMP assay exhibited 100% specificity for detecting 19 S. flexneri serotypes, no 96 strains of Shigella spp. and other bacterial pathogens. This LAMP assay was used to identify S. flexneri serotypes 2 and Xv from 299 S. flexneri strains isolated in China and results were consistent with that of slide agglutination and multiplex polymerase chain reaction results for the same isolates.
Conclusions
This LAMP assay may facilitate rapid and reliable identifying S. flexneri serotypes 2 and Xv.
Significance and Impact of Study
The present study was the first LAMP method for identifying serotypes of S. flexneri. |
| doi_str_mv | 10.1111/jam.15383 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2602641693</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2644357556</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3533-7e1ddc62103f382b0036b80b3ca655e77ac95e62606f81d22d0a6025546b7be23</originalsourceid><addsrcrecordid>eNp1kctKxDAUhoMoXkYXvoAE3OiiYy5N2lnKeEdxoYK7kranmiFtatKqs_MRfEafxIxVF4JnkwP58vGTH6FtSsY0zMFM1WMqeMqX0DrlUkRMJmz5a48jQRK2hja8nxFCORFyFa3xOGWMTtJ19HYEz2BsW0PTYVthhY217cfbew2lVh2UWHvbPYKrlcGqbo2udKE6bRusvFdzXFmHa2ug6I1y2IOz3bzVzcNCdvOoH8AYhSsDrw04jW-Ge_CYYdWU-P55E61UynjY-j5H6O7k-HZ6Fl1en55PDy-jggvOowRoWRaSUcIrnrKcEC7zlOS8UFIISBJVTARIJomsUloyVhIlCRMilnmSA-MjtDd4W2efevBdVmtfLNI1YHufhZdMxlROeEB3_6Az27smpAtUHHORCCEDtT9QhbPeO6iy1ulauXlGSbaoJQu1ZF-1BHbn29jn4WN_yZ8eAnAwAC_awPx_U3ZxeDUoPwGq5Jga</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2644357556</pqid></control><display><type>article</type><title>Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv</title><source>MEDLINE</source><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Li, Sha ; Ji, Shunshi ; Zhu, Xiong ; Chen, Hai ; Jin, Dong</creator><creatorcontrib>Li, Sha ; Ji, Shunshi ; Zhu, Xiong ; Chen, Hai ; Jin, Dong</creatorcontrib><description>Aims
This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv.
Methods and Results
The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gtrX, opt and wzx was developed. Its specificity and sensitivity were evaluated with 19 serotypes of S. flexneri and 96 other Shigella species and bacterial pathogens commonly found in stool samples. This LAMP assay was completed within 20 min at 61°C and could detect boiled DNA samples at concentrations as low as 1 pg/μl. The S. flexneri serotype LAMP assay exhibited 100% specificity for detecting 19 S. flexneri serotypes, no 96 strains of Shigella spp. and other bacterial pathogens. This LAMP assay was used to identify S. flexneri serotypes 2 and Xv from 299 S. flexneri strains isolated in China and results were consistent with that of slide agglutination and multiplex polymerase chain reaction results for the same isolates.
Conclusions
This LAMP assay may facilitate rapid and reliable identifying S. flexneri serotypes 2 and Xv.
Significance and Impact of Study
The present study was the first LAMP method for identifying serotypes of S. flexneri.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15383</identifier><identifier>PMID: 34822198</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Agglutination ; Amplification ; Antigens ; Assaying ; Deoxyribonucleic acid ; DNA ; loop‐mediated isothermal amplification1 ; Molecular Diagnostic Techniques ; Multiplex Polymerase Chain Reaction - methods ; Nucleic Acid Amplification Techniques ; Pathogens ; Polymerase chain reaction ; Sensitivity analysis ; sensitivity4 ; Serogroup ; serotype3 ; Serotypes ; Serotyping ; Serotyping - methods ; Shigella ; Shigella - genetics ; Shigella flexneri ; Shigella flexneri - genetics ; Shigella flexneri 2 ; specificity5 ; Strains (organisms)</subject><ispartof>Journal of applied microbiology, 2022-04, Vol.132 (4), p.2980-2989</ispartof><rights>2021 Society for Applied Microbiology.</rights><rights>Copyright © 2022 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-7e1ddc62103f382b0036b80b3ca655e77ac95e62606f81d22d0a6025546b7be23</citedby><cites>FETCH-LOGICAL-c3533-7e1ddc62103f382b0036b80b3ca655e77ac95e62606f81d22d0a6025546b7be23</cites><orcidid>0000-0001-9701-7674 ; 0000-0003-4588-1115</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15383$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15383$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34822198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Sha</creatorcontrib><creatorcontrib>Ji, Shunshi</creatorcontrib><creatorcontrib>Zhu, Xiong</creatorcontrib><creatorcontrib>Chen, Hai</creatorcontrib><creatorcontrib>Jin, Dong</creatorcontrib><title>Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv.
Methods and Results
The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gtrX, opt and wzx was developed. Its specificity and sensitivity were evaluated with 19 serotypes of S. flexneri and 96 other Shigella species and bacterial pathogens commonly found in stool samples. This LAMP assay was completed within 20 min at 61°C and could detect boiled DNA samples at concentrations as low as 1 pg/μl. The S. flexneri serotype LAMP assay exhibited 100% specificity for detecting 19 S. flexneri serotypes, no 96 strains of Shigella spp. and other bacterial pathogens. This LAMP assay was used to identify S. flexneri serotypes 2 and Xv from 299 S. flexneri strains isolated in China and results were consistent with that of slide agglutination and multiplex polymerase chain reaction results for the same isolates.
Conclusions
This LAMP assay may facilitate rapid and reliable identifying S. flexneri serotypes 2 and Xv.
Significance and Impact of Study
The present study was the first LAMP method for identifying serotypes of S. flexneri.</description><subject>Agglutination</subject><subject>Amplification</subject><subject>Antigens</subject><subject>Assaying</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>loop‐mediated isothermal amplification1</subject><subject>Molecular Diagnostic Techniques</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Sensitivity analysis</subject><subject>sensitivity4</subject><subject>Serogroup</subject><subject>serotype3</subject><subject>Serotypes</subject><subject>Serotyping</subject><subject>Serotyping - methods</subject><subject>Shigella</subject><subject>Shigella - genetics</subject><subject>Shigella flexneri</subject><subject>Shigella flexneri - genetics</subject><subject>Shigella flexneri 2</subject><subject>specificity5</subject><subject>Strains (organisms)</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctKxDAUhoMoXkYXvoAE3OiiYy5N2lnKeEdxoYK7kranmiFtatKqs_MRfEafxIxVF4JnkwP58vGTH6FtSsY0zMFM1WMqeMqX0DrlUkRMJmz5a48jQRK2hja8nxFCORFyFa3xOGWMTtJ19HYEz2BsW0PTYVthhY217cfbew2lVh2UWHvbPYKrlcGqbo2udKE6bRusvFdzXFmHa2ug6I1y2IOz3bzVzcNCdvOoH8AYhSsDrw04jW-Ge_CYYdWU-P55E61UynjY-j5H6O7k-HZ6Fl1en55PDy-jggvOowRoWRaSUcIrnrKcEC7zlOS8UFIISBJVTARIJomsUloyVhIlCRMilnmSA-MjtDd4W2efevBdVmtfLNI1YHufhZdMxlROeEB3_6Az27smpAtUHHORCCEDtT9QhbPeO6iy1ulauXlGSbaoJQu1ZF-1BHbn29jn4WN_yZ8eAnAwAC_awPx_U3ZxeDUoPwGq5Jga</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Li, Sha</creator><creator>Ji, Shunshi</creator><creator>Zhu, Xiong</creator><creator>Chen, Hai</creator><creator>Jin, Dong</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9701-7674</orcidid><orcidid>https://orcid.org/0000-0003-4588-1115</orcidid></search><sort><creationdate>202204</creationdate><title>Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv</title><author>Li, Sha ; Ji, Shunshi ; Zhu, Xiong ; Chen, Hai ; Jin, Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-7e1ddc62103f382b0036b80b3ca655e77ac95e62606f81d22d0a6025546b7be23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agglutination</topic><topic>Amplification</topic><topic>Antigens</topic><topic>Assaying</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>loop‐mediated isothermal amplification1</topic><topic>Molecular Diagnostic Techniques</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Sensitivity analysis</topic><topic>sensitivity4</topic><topic>Serogroup</topic><topic>serotype3</topic><topic>Serotypes</topic><topic>Serotyping</topic><topic>Serotyping - methods</topic><topic>Shigella</topic><topic>Shigella - genetics</topic><topic>Shigella flexneri</topic><topic>Shigella flexneri - genetics</topic><topic>Shigella flexneri 2</topic><topic>specificity5</topic><topic>Strains (organisms)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Sha</creatorcontrib><creatorcontrib>Ji, Shunshi</creatorcontrib><creatorcontrib>Zhu, Xiong</creatorcontrib><creatorcontrib>Chen, Hai</creatorcontrib><creatorcontrib>Jin, Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Sha</au><au>Ji, Shunshi</au><au>Zhu, Xiong</au><au>Chen, Hai</au><au>Jin, Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2022-04</date><risdate>2022</risdate><volume>132</volume><issue>4</issue><spage>2980</spage><epage>2989</epage><pages>2980-2989</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This study developed and evaluated a loop‐mediated isothermal amplification (LAMP) assay to simply, rapidly and accurately identify Shigella flexneri serotypes 2 and Xv.
Methods and Results
The LAMP assay based on the O‐antigen synthesis and modification genes of S. flexneri including gtrII, gtrX, opt and wzx was developed. Its specificity and sensitivity were evaluated with 19 serotypes of S. flexneri and 96 other Shigella species and bacterial pathogens commonly found in stool samples. This LAMP assay was completed within 20 min at 61°C and could detect boiled DNA samples at concentrations as low as 1 pg/μl. The S. flexneri serotype LAMP assay exhibited 100% specificity for detecting 19 S. flexneri serotypes, no 96 strains of Shigella spp. and other bacterial pathogens. This LAMP assay was used to identify S. flexneri serotypes 2 and Xv from 299 S. flexneri strains isolated in China and results were consistent with that of slide agglutination and multiplex polymerase chain reaction results for the same isolates.
Conclusions
This LAMP assay may facilitate rapid and reliable identifying S. flexneri serotypes 2 and Xv.
Significance and Impact of Study
The present study was the first LAMP method for identifying serotypes of S. flexneri.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>34822198</pmid><doi>10.1111/jam.15383</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9701-7674</orcidid><orcidid>https://orcid.org/0000-0003-4588-1115</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1364-5072 |
| ispartof | Journal of applied microbiology, 2022-04, Vol.132 (4), p.2980-2989 |
| issn | 1364-5072 1365-2672 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2602641693 |
| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Agglutination Amplification Antigens Assaying Deoxyribonucleic acid DNA loop‐mediated isothermal amplification1 Molecular Diagnostic Techniques Multiplex Polymerase Chain Reaction - methods Nucleic Acid Amplification Techniques Pathogens Polymerase chain reaction Sensitivity analysis sensitivity4 Serogroup serotype3 Serotypes Serotyping Serotyping - methods Shigella Shigella - genetics Shigella flexneri Shigella flexneri - genetics Shigella flexneri 2 specificity5 Strains (organisms) |
| title | Development of a loop‐mediated isothermal amplification assay for molecular serotyping of Shigella flexneri Serotypes 2 and Xv |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T14%3A13%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20loop%E2%80%90mediated%20isothermal%20amplification%20assay%20for%20molecular%20serotyping%20of%20Shigella%20flexneri%20Serotypes%202%20and%20Xv&rft.jtitle=Journal%20of%20applied%20microbiology&rft.au=Li,%20Sha&rft.date=2022-04&rft.volume=132&rft.issue=4&rft.spage=2980&rft.epage=2989&rft.pages=2980-2989&rft.issn=1364-5072&rft.eissn=1365-2672&rft_id=info:doi/10.1111/jam.15383&rft_dat=%3Cproquest_cross%3E2644357556%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2644357556&rft_id=info:pmid/34822198&rfr_iscdi=true |