Post‐autotomy regeneration of respiratory tree in sea cucumber Holothuria parva
The respiratory trees present only in the class Holothuroidea and the rest of the echinoderms lack it. Only some holothurian species have the ability to regenerate their respiratory trees after autotomy. Therefore, respiratory trees could be considered as a suitable model to assess the regeneration...
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Veröffentlicht in: | Journal of experimental zoology. Part B, Molecular and developmental evolution Molecular and developmental evolution, 2022-05, Vol.338 (3), p.155-169 |
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creator | Eisapour, Mina Salamat, Negin Salari, Mohammad Ali Bahabadi, Mahmoud Nafisi Salati, Amir Parviz |
description | The respiratory trees present only in the class Holothuroidea and the rest of the echinoderms lack it. Only some holothurian species have the ability to regenerate their respiratory trees after autotomy. Therefore, respiratory trees could be considered as a suitable model to assess the regeneration mechanisms in animals. In the present study, the respiratory tree regeneration after posterior evisceration were examined in Holothuria parva during 75 days. Since autotomy reduces antioxidant defense in the organisms, in the present study alterations of antioxidant enzymes were also evaluated during the experiment. H. parva is the dominant intertidal species distributed in the north of the Persian Gulf. In the present study, H. parva ejected the left respiratory tree, the digestive tract and supportive mesenteries from the anus, about 1–2 min after potassium chloride injection. The closure of the opening at the posterior ends of the body was the first reaction to the injury. Seven days after evisceration, the small bud formed on the dorsal side of the cloaca which was covered with the coelomic epithelium of cloaca. The coelomic epithelium started to proliferate to undifferentiated cells on the apex of the buds. The primary respiratory tree consisted of the luminal cuboidal epithelium and thin connective tissue surrounded by the slender coelomic epithelium. This preliminary organ was observed at the apex of the buds, 13 days after evisceration. Gradually, myoepithelial cells arranged around a longitudinal axis and formed a circular muscle. The primitive branches of primary respiratory tree started to form 18 days after evisceration. Forty days after evisceration, the luminal epithelium of the respiratory tree had the same appearance as the intact luminal epithelium. The regenerated respiratory tree was histomorphologically very similar to an intact respiratory tree 56 days postevisceration, but unlike that, it was not yet wrapped around the intestine and was completely separate from it. Despite the development of the regenerating respiratory tree, no wrapping around the intestine was observed until the end of the experiment. According to the results, the activity of the catalase (CAT) and superoxide dismutase (SOD) in the muscle homogenate was significantly higher than the control 5 days after evisceration. The CAT and SOD levels gradually decreased in eviscerated animals. The lipid peroxidation level followed a decreasing trend in the eviscerated animals during |
doi_str_mv | 10.1002/jez.b.23109 |
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Graphical
HIGHLIGHTS
Holothuria parva is posterior‐eviscerating sea cucumber.
Only the left respiratory tree of Holothuria parva was eviscereted.
The respiratory tree of Holothuria parva was only regenerated from cloacal end.
56 dpe, the tissue structure of regenerated respiratory tree was similar to intact digestive tract.
75 dpe, regenerated respiratory tree was not yet wrapped around the intestine.
CAT and SOD in the regenerated respiratory tree was significantly higher than the control</description><identifier>ISSN: 1552-5007</identifier><identifier>EISSN: 1552-5015</identifier><identifier>DOI: 10.1002/jez.b.23109</identifier><identifier>PMID: 34813182</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antioxidants ; evisceration ; Holothuria - physiology ; Holothuria parva ; holothurian ; regeneration ; respiratory trees ; Sea Cucumbers ; Superoxide Dismutase ; Trees</subject><ispartof>Journal of experimental zoology. Part B, Molecular and developmental evolution, 2022-05, Vol.338 (3), p.155-169</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2629-7ccccd24dfcf28120dcf1013fbb2113cd4e20316a92197bb49cc00e31e43ed453</citedby><cites>FETCH-LOGICAL-c2629-7ccccd24dfcf28120dcf1013fbb2113cd4e20316a92197bb49cc00e31e43ed453</cites><orcidid>0000-0002-5565-3725</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjez.b.23109$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjez.b.23109$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34813182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eisapour, Mina</creatorcontrib><creatorcontrib>Salamat, Negin</creatorcontrib><creatorcontrib>Salari, Mohammad Ali</creatorcontrib><creatorcontrib>Bahabadi, Mahmoud Nafisi</creatorcontrib><creatorcontrib>Salati, Amir Parviz</creatorcontrib><title>Post‐autotomy regeneration of respiratory tree in sea cucumber Holothuria parva</title><title>Journal of experimental zoology. Part B, Molecular and developmental evolution</title><addtitle>J Exp Zool B Mol Dev Evol</addtitle><description>The respiratory trees present only in the class Holothuroidea and the rest of the echinoderms lack it. Only some holothurian species have the ability to regenerate their respiratory trees after autotomy. Therefore, respiratory trees could be considered as a suitable model to assess the regeneration mechanisms in animals. In the present study, the respiratory tree regeneration after posterior evisceration were examined in Holothuria parva during 75 days. Since autotomy reduces antioxidant defense in the organisms, in the present study alterations of antioxidant enzymes were also evaluated during the experiment. H. parva is the dominant intertidal species distributed in the north of the Persian Gulf. In the present study, H. parva ejected the left respiratory tree, the digestive tract and supportive mesenteries from the anus, about 1–2 min after potassium chloride injection. The closure of the opening at the posterior ends of the body was the first reaction to the injury. Seven days after evisceration, the small bud formed on the dorsal side of the cloaca which was covered with the coelomic epithelium of cloaca. The coelomic epithelium started to proliferate to undifferentiated cells on the apex of the buds. The primary respiratory tree consisted of the luminal cuboidal epithelium and thin connective tissue surrounded by the slender coelomic epithelium. This preliminary organ was observed at the apex of the buds, 13 days after evisceration. Gradually, myoepithelial cells arranged around a longitudinal axis and formed a circular muscle. The primitive branches of primary respiratory tree started to form 18 days after evisceration. Forty days after evisceration, the luminal epithelium of the respiratory tree had the same appearance as the intact luminal epithelium. The regenerated respiratory tree was histomorphologically very similar to an intact respiratory tree 56 days postevisceration, but unlike that, it was not yet wrapped around the intestine and was completely separate from it. Despite the development of the regenerating respiratory tree, no wrapping around the intestine was observed until the end of the experiment. According to the results, the activity of the catalase (CAT) and superoxide dismutase (SOD) in the muscle homogenate was significantly higher than the control 5 days after evisceration. The CAT and SOD levels gradually decreased in eviscerated animals. The lipid peroxidation level followed a decreasing trend in the eviscerated animals during the experiment. However, its value reduced to the control level at the end of the experiment.
Graphical
HIGHLIGHTS
Holothuria parva is posterior‐eviscerating sea cucumber.
Only the left respiratory tree of Holothuria parva was eviscereted.
The respiratory tree of Holothuria parva was only regenerated from cloacal end.
56 dpe, the tissue structure of regenerated respiratory tree was similar to intact digestive tract.
75 dpe, regenerated respiratory tree was not yet wrapped around the intestine.
CAT and SOD in the regenerated respiratory tree was significantly higher than the control</description><subject>Animals</subject><subject>Antioxidants</subject><subject>evisceration</subject><subject>Holothuria - physiology</subject><subject>Holothuria parva</subject><subject>holothurian</subject><subject>regeneration</subject><subject>respiratory trees</subject><subject>Sea Cucumbers</subject><subject>Superoxide Dismutase</subject><subject>Trees</subject><issn>1552-5007</issn><issn>1552-5015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKw0AQhhdRbK2evEuOgqTu7CZpctRSrVJQQS9els1moilJNu4mSjz5CD6jT-Jqa4_OZeaHjx_mI-QQ6BgoZadLfB-nY8aBJltkCGHI_JBCuL256WRA9qxdOjiiYbhLBjyIgUPMhuTuVtv26-NTdq1uddV7Bp-wRiPbQteezl22TeGiNr3XGkSvqD2L0lOd6qoUjTfXpW6fO1NIr5HmVe6TnVyWFg_We0QeLmb307m_uLm8mp4tfMUilvgT5SZjQZarnMXAaKZyoMDzNGUAXGUBMsohkgmDZJKmQaIUpcgBA45ZEPIROV71Nka_dGhbURVWYVnKGnVnhfsVgphHUezQkxWqjLbWYC4aU1TS9AKo-HEonEORil-Hjj5aF3dphdmG_ZPmALYC3ooS-_-6xPXs8XzV-g0ieX7K</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Eisapour, Mina</creator><creator>Salamat, Negin</creator><creator>Salari, Mohammad Ali</creator><creator>Bahabadi, Mahmoud Nafisi</creator><creator>Salati, Amir Parviz</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5565-3725</orcidid></search><sort><creationdate>202205</creationdate><title>Post‐autotomy regeneration of respiratory tree in sea cucumber Holothuria parva</title><author>Eisapour, Mina ; Salamat, Negin ; Salari, Mohammad Ali ; Bahabadi, Mahmoud Nafisi ; Salati, Amir Parviz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2629-7ccccd24dfcf28120dcf1013fbb2113cd4e20316a92197bb49cc00e31e43ed453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>evisceration</topic><topic>Holothuria - physiology</topic><topic>Holothuria parva</topic><topic>holothurian</topic><topic>regeneration</topic><topic>respiratory trees</topic><topic>Sea Cucumbers</topic><topic>Superoxide Dismutase</topic><topic>Trees</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eisapour, Mina</creatorcontrib><creatorcontrib>Salamat, Negin</creatorcontrib><creatorcontrib>Salari, Mohammad Ali</creatorcontrib><creatorcontrib>Bahabadi, Mahmoud Nafisi</creatorcontrib><creatorcontrib>Salati, Amir Parviz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental zoology. Part B, Molecular and developmental evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eisapour, Mina</au><au>Salamat, Negin</au><au>Salari, Mohammad Ali</au><au>Bahabadi, Mahmoud Nafisi</au><au>Salati, Amir Parviz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Post‐autotomy regeneration of respiratory tree in sea cucumber Holothuria parva</atitle><jtitle>Journal of experimental zoology. Part B, Molecular and developmental evolution</jtitle><addtitle>J Exp Zool B Mol Dev Evol</addtitle><date>2022-05</date><risdate>2022</risdate><volume>338</volume><issue>3</issue><spage>155</spage><epage>169</epage><pages>155-169</pages><issn>1552-5007</issn><eissn>1552-5015</eissn><abstract>The respiratory trees present only in the class Holothuroidea and the rest of the echinoderms lack it. Only some holothurian species have the ability to regenerate their respiratory trees after autotomy. Therefore, respiratory trees could be considered as a suitable model to assess the regeneration mechanisms in animals. In the present study, the respiratory tree regeneration after posterior evisceration were examined in Holothuria parva during 75 days. Since autotomy reduces antioxidant defense in the organisms, in the present study alterations of antioxidant enzymes were also evaluated during the experiment. H. parva is the dominant intertidal species distributed in the north of the Persian Gulf. In the present study, H. parva ejected the left respiratory tree, the digestive tract and supportive mesenteries from the anus, about 1–2 min after potassium chloride injection. The closure of the opening at the posterior ends of the body was the first reaction to the injury. Seven days after evisceration, the small bud formed on the dorsal side of the cloaca which was covered with the coelomic epithelium of cloaca. The coelomic epithelium started to proliferate to undifferentiated cells on the apex of the buds. The primary respiratory tree consisted of the luminal cuboidal epithelium and thin connective tissue surrounded by the slender coelomic epithelium. This preliminary organ was observed at the apex of the buds, 13 days after evisceration. Gradually, myoepithelial cells arranged around a longitudinal axis and formed a circular muscle. The primitive branches of primary respiratory tree started to form 18 days after evisceration. Forty days after evisceration, the luminal epithelium of the respiratory tree had the same appearance as the intact luminal epithelium. The regenerated respiratory tree was histomorphologically very similar to an intact respiratory tree 56 days postevisceration, but unlike that, it was not yet wrapped around the intestine and was completely separate from it. Despite the development of the regenerating respiratory tree, no wrapping around the intestine was observed until the end of the experiment. According to the results, the activity of the catalase (CAT) and superoxide dismutase (SOD) in the muscle homogenate was significantly higher than the control 5 days after evisceration. The CAT and SOD levels gradually decreased in eviscerated animals. The lipid peroxidation level followed a decreasing trend in the eviscerated animals during the experiment. However, its value reduced to the control level at the end of the experiment.
Graphical
HIGHLIGHTS
Holothuria parva is posterior‐eviscerating sea cucumber.
Only the left respiratory tree of Holothuria parva was eviscereted.
The respiratory tree of Holothuria parva was only regenerated from cloacal end.
56 dpe, the tissue structure of regenerated respiratory tree was similar to intact digestive tract.
75 dpe, regenerated respiratory tree was not yet wrapped around the intestine.
CAT and SOD in the regenerated respiratory tree was significantly higher than the control</abstract><cop>United States</cop><pmid>34813182</pmid><doi>10.1002/jez.b.23109</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-5565-3725</orcidid></addata></record> |
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language | eng |
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source | MEDLINE; Wiley Online Library |
subjects | Animals Antioxidants evisceration Holothuria - physiology Holothuria parva holothurian regeneration respiratory trees Sea Cucumbers Superoxide Dismutase Trees |
title | Post‐autotomy regeneration of respiratory tree in sea cucumber Holothuria parva |
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