Poly(vinyl alcohol)‐methacrylate with CRGD peptide: A photocurable biocompatible hydrogel
Polyvinyl alcohol (PVA)‐based hydrogels are promising biomaterials for tissue engineering printing applications. However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was...
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| Veröffentlicht in: | Journal of tissue engineering and regenerative medicine 2022-02, Vol.16 (2), p.140-150 |
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| container_title | Journal of tissue engineering and regenerative medicine |
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| creator | Goldvaser, Michael Epstein, Eyal Rosen, Osnat Jayson, Avital Natan, Niva Ben‐Shalom, Tal Saphier, Sigal Katalan, Shahaf Shoseyov, Oded |
| description | Polyvinyl alcohol (PVA)‐based hydrogels are promising biomaterials for tissue engineering printing applications. However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was to develop a printable, cell‐supportive PVA‐based bioink with tunable mechanical properties, without using animal‐derived polymers which potentially harbor human pathogens. An ultraviolet light (UV) curable PVA‐methacrylate (PVA‐MA) polymer mixed with Cys‐Arg‐Gly‐Asp (CRGD) peptide was developed. This peptide holds the integrin receptor binding sequence – RGD, that can enhance cell attachment. The additional cysteine was designed to enable its thiol binding under UV to methacrylate groups of the UV curable PVA‐MA. Vero cell, as an adherent cell model was used to assess the hydrogel's cell adhesion. It was found that the PVA‐MA‐CRGD formula enables the preparation of hydrogels with excellent cell attachment and had even shown superior cell attachment properties relative to added gelatin. Adding hyaluronic acid (HA) as a rheologic modulator enabled the printing of this new formula. Our overall data demonstrates the applicability of this mixture as a bioink for soft tissue engineering such as skin, adipose, liver or kidney tissue. |
| doi_str_mv | 10.1002/term.3265 |
| format | Article |
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However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was to develop a printable, cell‐supportive PVA‐based bioink with tunable mechanical properties, without using animal‐derived polymers which potentially harbor human pathogens. An ultraviolet light (UV) curable PVA‐methacrylate (PVA‐MA) polymer mixed with Cys‐Arg‐Gly‐Asp (CRGD) peptide was developed. This peptide holds the integrin receptor binding sequence – RGD, that can enhance cell attachment. The additional cysteine was designed to enable its thiol binding under UV to methacrylate groups of the UV curable PVA‐MA. Vero cell, as an adherent cell model was used to assess the hydrogel's cell adhesion. It was found that the PVA‐MA‐CRGD formula enables the preparation of hydrogels with excellent cell attachment and had even shown superior cell attachment properties relative to added gelatin. Adding hyaluronic acid (HA) as a rheologic modulator enabled the printing of this new formula. Our overall data demonstrates the applicability of this mixture as a bioink for soft tissue engineering such as skin, adipose, liver or kidney tissue.</description><identifier>ISSN: 1932-6254</identifier><identifier>EISSN: 1932-7005</identifier><identifier>DOI: 10.1002/term.3265</identifier><identifier>PMID: 34808035</identifier><language>eng</language><publisher>England: Hindawi Limited</publisher><subject>Attachment ; Binding ; Biocompatibility ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; bioink ; Biomaterials ; Biomedical materials ; Cell adhesion ; cell attachment ; Gelatin ; Humans ; Hyaluronic acid ; Hydrogels ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Kidneys ; Mechanical properties ; Methacrylates - chemistry ; Methacrylates - pharmacology ; Peptides ; Peptides, Cyclic ; Photocuring ; Polymers ; Polyvinyl alcohol ; Polyvinyl Alcohol - chemistry ; Polyvinyl Alcohol - pharmacology ; PVA ; Regenerative medicine ; RGD ; Rheological properties ; scaffold ; Soft tissues ; Tissue Engineering ; Ultraviolet radiation</subject><ispartof>Journal of tissue engineering and regenerative medicine, 2022-02, Vol.16 (2), p.140-150</ispartof><rights>2021 John Wiley & Sons Ltd.</rights><rights>2022 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3035-e3947a89a6a30ca5969f94f03c31eed6cb73b8d187cebd836a7ba4ce31c87ea63</citedby><cites>FETCH-LOGICAL-c3035-e3947a89a6a30ca5969f94f03c31eed6cb73b8d187cebd836a7ba4ce31c87ea63</cites><orcidid>0000-0003-4855-2819</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fterm.3265$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fterm.3265$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34808035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goldvaser, Michael</creatorcontrib><creatorcontrib>Epstein, Eyal</creatorcontrib><creatorcontrib>Rosen, Osnat</creatorcontrib><creatorcontrib>Jayson, Avital</creatorcontrib><creatorcontrib>Natan, Niva</creatorcontrib><creatorcontrib>Ben‐Shalom, Tal</creatorcontrib><creatorcontrib>Saphier, Sigal</creatorcontrib><creatorcontrib>Katalan, Shahaf</creatorcontrib><creatorcontrib>Shoseyov, Oded</creatorcontrib><title>Poly(vinyl alcohol)‐methacrylate with CRGD peptide: A photocurable biocompatible hydrogel</title><title>Journal of tissue engineering and regenerative medicine</title><addtitle>J Tissue Eng Regen Med</addtitle><description>Polyvinyl alcohol (PVA)‐based hydrogels are promising biomaterials for tissue engineering printing applications. However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was to develop a printable, cell‐supportive PVA‐based bioink with tunable mechanical properties, without using animal‐derived polymers which potentially harbor human pathogens. An ultraviolet light (UV) curable PVA‐methacrylate (PVA‐MA) polymer mixed with Cys‐Arg‐Gly‐Asp (CRGD) peptide was developed. This peptide holds the integrin receptor binding sequence – RGD, that can enhance cell attachment. The additional cysteine was designed to enable its thiol binding under UV to methacrylate groups of the UV curable PVA‐MA. Vero cell, as an adherent cell model was used to assess the hydrogel's cell adhesion. It was found that the PVA‐MA‐CRGD formula enables the preparation of hydrogels with excellent cell attachment and had even shown superior cell attachment properties relative to added gelatin. Adding hyaluronic acid (HA) as a rheologic modulator enabled the printing of this new formula. Our overall data demonstrates the applicability of this mixture as a bioink for soft tissue engineering such as skin, adipose, liver or kidney tissue.</description><subject>Attachment</subject><subject>Binding</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>bioink</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cell adhesion</subject><subject>cell attachment</subject><subject>Gelatin</subject><subject>Humans</subject><subject>Hyaluronic acid</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Kidneys</subject><subject>Mechanical properties</subject><subject>Methacrylates - chemistry</subject><subject>Methacrylates - pharmacology</subject><subject>Peptides</subject><subject>Peptides, Cyclic</subject><subject>Photocuring</subject><subject>Polymers</subject><subject>Polyvinyl alcohol</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Polyvinyl Alcohol - pharmacology</subject><subject>PVA</subject><subject>Regenerative medicine</subject><subject>RGD</subject><subject>Rheological properties</subject><subject>scaffold</subject><subject>Soft tissues</subject><subject>Tissue Engineering</subject><subject>Ultraviolet radiation</subject><issn>1932-6254</issn><issn>1932-7005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E9PwjAYBvDGaATRg1_ALPECB6Bdt67zRhDRBKMhePKwdN2LG-no7DbJbn4EP6OfxCLowcRT_-SXJ-_7IHRO8IBg7A4rMPmAusw_QG0SUrcfYOwf7u_M9b0WOinLlf30mU-PUYt6HHNM_TZ6ftSq6b5l60Y5QkmdatX7fP_IoUqFNI0SFTibrEqd8Xx67RRQVFkCV87IKVJdaVkbEStw4kxLnReiyravtEmMfgF1io6WQpVwtj876Olmshjf9mcP07vxaNaX1M7QBxp6geChYIJiKfyQhcvQW2IqKQFImIwDGvOE8EBCnHDKRBALTwIlkgcgGO2g7i63MPq1hrKK8qyUoJRYg67LyGWY2IUJI5Ze_qErXZu1nc4q18Oc-dyzqrdT0uiyNLCMCpPlwjQRwdG28WjbeLRt3NqLfWId55D8yp-KLRjuwCZT0PyfFC0m8_vvyC8Q_YxS</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Goldvaser, Michael</creator><creator>Epstein, Eyal</creator><creator>Rosen, Osnat</creator><creator>Jayson, Avital</creator><creator>Natan, Niva</creator><creator>Ben‐Shalom, Tal</creator><creator>Saphier, Sigal</creator><creator>Katalan, Shahaf</creator><creator>Shoseyov, Oded</creator><general>Hindawi Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4855-2819</orcidid></search><sort><creationdate>202202</creationdate><title>Poly(vinyl alcohol)‐methacrylate with CRGD peptide: A photocurable biocompatible hydrogel</title><author>Goldvaser, Michael ; Epstein, Eyal ; Rosen, Osnat ; Jayson, Avital ; Natan, Niva ; Ben‐Shalom, Tal ; Saphier, Sigal ; Katalan, Shahaf ; Shoseyov, Oded</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3035-e3947a89a6a30ca5969f94f03c31eed6cb73b8d187cebd836a7ba4ce31c87ea63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Attachment</topic><topic>Binding</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>bioink</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Cell adhesion</topic><topic>cell attachment</topic><topic>Gelatin</topic><topic>Humans</topic><topic>Hyaluronic acid</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Hydrogels - pharmacology</topic><topic>Kidneys</topic><topic>Mechanical properties</topic><topic>Methacrylates - chemistry</topic><topic>Methacrylates - pharmacology</topic><topic>Peptides</topic><topic>Peptides, Cyclic</topic><topic>Photocuring</topic><topic>Polymers</topic><topic>Polyvinyl alcohol</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Polyvinyl Alcohol - pharmacology</topic><topic>PVA</topic><topic>Regenerative medicine</topic><topic>RGD</topic><topic>Rheological properties</topic><topic>scaffold</topic><topic>Soft tissues</topic><topic>Tissue Engineering</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goldvaser, Michael</creatorcontrib><creatorcontrib>Epstein, Eyal</creatorcontrib><creatorcontrib>Rosen, Osnat</creatorcontrib><creatorcontrib>Jayson, Avital</creatorcontrib><creatorcontrib>Natan, Niva</creatorcontrib><creatorcontrib>Ben‐Shalom, Tal</creatorcontrib><creatorcontrib>Saphier, Sigal</creatorcontrib><creatorcontrib>Katalan, Shahaf</creatorcontrib><creatorcontrib>Shoseyov, Oded</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of tissue engineering and regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goldvaser, Michael</au><au>Epstein, Eyal</au><au>Rosen, Osnat</au><au>Jayson, Avital</au><au>Natan, Niva</au><au>Ben‐Shalom, Tal</au><au>Saphier, Sigal</au><au>Katalan, Shahaf</au><au>Shoseyov, Oded</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly(vinyl alcohol)‐methacrylate with CRGD peptide: A photocurable biocompatible hydrogel</atitle><jtitle>Journal of tissue engineering and regenerative medicine</jtitle><addtitle>J Tissue Eng Regen Med</addtitle><date>2022-02</date><risdate>2022</risdate><volume>16</volume><issue>2</issue><spage>140</spage><epage>150</epage><pages>140-150</pages><issn>1932-6254</issn><eissn>1932-7005</eissn><abstract>Polyvinyl alcohol (PVA)‐based hydrogels are promising biomaterials for tissue engineering printing applications. However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was to develop a printable, cell‐supportive PVA‐based bioink with tunable mechanical properties, without using animal‐derived polymers which potentially harbor human pathogens. An ultraviolet light (UV) curable PVA‐methacrylate (PVA‐MA) polymer mixed with Cys‐Arg‐Gly‐Asp (CRGD) peptide was developed. This peptide holds the integrin receptor binding sequence – RGD, that can enhance cell attachment. The additional cysteine was designed to enable its thiol binding under UV to methacrylate groups of the UV curable PVA‐MA. Vero cell, as an adherent cell model was used to assess the hydrogel's cell adhesion. It was found that the PVA‐MA‐CRGD formula enables the preparation of hydrogels with excellent cell attachment and had even shown superior cell attachment properties relative to added gelatin. 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| identifier | ISSN: 1932-6254 |
| ispartof | Journal of tissue engineering and regenerative medicine, 2022-02, Vol.16 (2), p.140-150 |
| issn | 1932-6254 1932-7005 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Attachment Binding Biocompatibility Biocompatible Materials - chemistry Biocompatible Materials - pharmacology bioink Biomaterials Biomedical materials Cell adhesion cell attachment Gelatin Humans Hyaluronic acid Hydrogels Hydrogels - chemistry Hydrogels - pharmacology Kidneys Mechanical properties Methacrylates - chemistry Methacrylates - pharmacology Peptides Peptides, Cyclic Photocuring Polymers Polyvinyl alcohol Polyvinyl Alcohol - chemistry Polyvinyl Alcohol - pharmacology PVA Regenerative medicine RGD Rheological properties scaffold Soft tissues Tissue Engineering Ultraviolet radiation |
| title | Poly(vinyl alcohol)‐methacrylate with CRGD peptide: A photocurable biocompatible hydrogel |
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