Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging
Significance: Two-photon microscopy has become the standard platform for deep-tissue fluorescence imaging. However, the use of point scanning in conventional two-photon microscopy limits the speed of volumetric image acquisition. Aim: To obtain fast and deep volumetric images, we combine two-photon...
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| Veröffentlicht in: | Journal of biomedical optics 2021-11, Vol.26 (11), p.116503-116503 |
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| creator | Lin, Po-Yen Hwang, Sheng-Ping L Lee, Chi-Hon Chen, Bi-Chang |
| description | Significance: Two-photon microscopy has become the standard platform for deep-tissue fluorescence imaging. However, the use of point scanning in conventional two-photon microscopy limits the speed of volumetric image acquisition.
Aim: To obtain fast and deep volumetric images, we combine two-photon light sheet fluorescence microscopy (2p-LSFM) and axicon imaging that yields an extended depth of field (DOF) in 2p-LSFM.
Approach: Axicon imaging is achieved by imposing an axicon lens in the detection part of LSFM.
Results: The DOF with axicon imaging is extended more than 20-fold over that of a conventional imaging lens, liberating the synchronized scanning in LSFM. We captured images of dynamic beating hearts and red blood cells in zebrafish larvae at volume acquisition rates up to 30 Hz.
Conclusions: We demonstrate the fast three-dimensional imaging capability of 2p-LSFM with axicon imaging by recording the rapid dynamics of physiological processes. |
| doi_str_mv | 10.1117/1.JBO.26.11.116503 |
| format | Article |
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Aim: To obtain fast and deep volumetric images, we combine two-photon light sheet fluorescence microscopy (2p-LSFM) and axicon imaging that yields an extended depth of field (DOF) in 2p-LSFM.
Approach: Axicon imaging is achieved by imposing an axicon lens in the detection part of LSFM.
Results: The DOF with axicon imaging is extended more than 20-fold over that of a conventional imaging lens, liberating the synchronized scanning in LSFM. We captured images of dynamic beating hearts and red blood cells in zebrafish larvae at volume acquisition rates up to 30 Hz.
Conclusions: We demonstrate the fast three-dimensional imaging capability of 2p-LSFM with axicon imaging by recording the rapid dynamics of physiological processes.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.26.11.116503</identifier><identifier>PMID: 34796706</identifier><language>eng</language><publisher>Bellingham: Society of Photo-Optical Instrumentation Engineers</publisher><subject>Aperture ; Biological research ; Cameras ; Depth of field ; Erythrocytes ; Fluorescence ; Fluorescence microscopy ; Image acquisition ; Larvae ; Laser microscopy ; Lasers ; Lenses ; Light ; Light sheets ; Microscopy ; Photons ; Scanning ; Three dimensional imaging ; Zebrafish</subject><ispartof>Journal of biomedical optics, 2021-11, Vol.26 (11), p.116503-116503</ispartof><rights>The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.</rights><rights>2021. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 The Authors 2021 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-a673713e45a603b28e45f066c07a6f1cefc8edb34b894b4571af78cbcb715d4b3</citedby><orcidid>0000-0002-6138-711X ; 0000-0002-1876-7432</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2862327149/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2862327149?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,21369,27903,27904,33723,33724,43784,53769,53771,74048</link.rule.ids></links><search><creatorcontrib>Lin, Po-Yen</creatorcontrib><creatorcontrib>Hwang, Sheng-Ping L</creatorcontrib><creatorcontrib>Lee, Chi-Hon</creatorcontrib><creatorcontrib>Chen, Bi-Chang</creatorcontrib><title>Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging</title><title>Journal of biomedical optics</title><addtitle>J. Biomed. Opt</addtitle><description>Significance: Two-photon microscopy has become the standard platform for deep-tissue fluorescence imaging. However, the use of point scanning in conventional two-photon microscopy limits the speed of volumetric image acquisition.
Aim: To obtain fast and deep volumetric images, we combine two-photon light sheet fluorescence microscopy (2p-LSFM) and axicon imaging that yields an extended depth of field (DOF) in 2p-LSFM.
Approach: Axicon imaging is achieved by imposing an axicon lens in the detection part of LSFM.
Results: The DOF with axicon imaging is extended more than 20-fold over that of a conventional imaging lens, liberating the synchronized scanning in LSFM. We captured images of dynamic beating hearts and red blood cells in zebrafish larvae at volume acquisition rates up to 30 Hz.
Conclusions: We demonstrate the fast three-dimensional imaging capability of 2p-LSFM with axicon imaging by recording the rapid dynamics of physiological processes.</description><subject>Aperture</subject><subject>Biological research</subject><subject>Cameras</subject><subject>Depth of field</subject><subject>Erythrocytes</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Image acquisition</subject><subject>Larvae</subject><subject>Laser microscopy</subject><subject>Lasers</subject><subject>Lenses</subject><subject>Light</subject><subject>Light sheets</subject><subject>Microscopy</subject><subject>Photons</subject><subject>Scanning</subject><subject>Three dimensional imaging</subject><subject>Zebrafish</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9UU1vFSEUJUbT1to_4IrEjZt55QIDzMZEG7U2Tbqpa8Lw4A3NzDAC049_L82rNnZhQsK9ueecnHsPQu-BbABAnsLm4svVhora1Sdawl6hI2gFaShV8LrWRLGGCaEO0ducbwghSnTiAB0yLjshiThC_vouNssQS5xxtmae3RaPYTcUnAfnCvbjGpPL1s3W4SnYFLONywO-C2XA5j7YyguT2YV5h31M2Jtc8G0c18mVFOyf2Tv0xpsxu5On_xj9_Pb1-uy8ubz6_uPs82VjOZelMUIyCczx1gjCeqpq5YkQlkgjPFjnrXLbnvFedbznrQTjpbK97SW0W96zY_Rpr7us_eS21XZJZtRLqj7Sg44m6H8ncxj0Lt5qJQhwBlXg45NAir9Wl4ueQt1-HM3s4po1bbsOFCjBKvTDC-hNXNNc19NUCcqoBN5VFN2jHk-Xk_N_zQDRjzFq0DVGTUXt9D7GSjrdk_IS3LPsfxi_AQQ8n8c</recordid><startdate>20211101</startdate><enddate>20211101</enddate><creator>Lin, Po-Yen</creator><creator>Hwang, Sheng-Ping L</creator><creator>Lee, Chi-Hon</creator><creator>Chen, Bi-Chang</creator><general>Society of Photo-Optical Instrumentation Engineers</general><general>S P I E - International Society for</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6138-711X</orcidid><orcidid>https://orcid.org/0000-0002-1876-7432</orcidid></search><sort><creationdate>20211101</creationdate><title>Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging</title><author>Lin, Po-Yen ; Hwang, Sheng-Ping L ; Lee, Chi-Hon ; Chen, Bi-Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-a673713e45a603b28e45f066c07a6f1cefc8edb34b894b4571af78cbcb715d4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Aperture</topic><topic>Biological research</topic><topic>Cameras</topic><topic>Depth of field</topic><topic>Erythrocytes</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Image acquisition</topic><topic>Larvae</topic><topic>Laser microscopy</topic><topic>Lasers</topic><topic>Lenses</topic><topic>Light</topic><topic>Light sheets</topic><topic>Microscopy</topic><topic>Photons</topic><topic>Scanning</topic><topic>Three dimensional imaging</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Po-Yen</creatorcontrib><creatorcontrib>Hwang, Sheng-Ping L</creatorcontrib><creatorcontrib>Lee, Chi-Hon</creatorcontrib><creatorcontrib>Chen, Bi-Chang</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Po-Yen</au><au>Hwang, Sheng-Ping L</au><au>Lee, Chi-Hon</au><au>Chen, Bi-Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2021-11-01</date><risdate>2021</risdate><volume>26</volume><issue>11</issue><spage>116503</spage><epage>116503</epage><pages>116503-116503</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>Significance: Two-photon microscopy has become the standard platform for deep-tissue fluorescence imaging. However, the use of point scanning in conventional two-photon microscopy limits the speed of volumetric image acquisition.
Aim: To obtain fast and deep volumetric images, we combine two-photon light sheet fluorescence microscopy (2p-LSFM) and axicon imaging that yields an extended depth of field (DOF) in 2p-LSFM.
Approach: Axicon imaging is achieved by imposing an axicon lens in the detection part of LSFM.
Results: The DOF with axicon imaging is extended more than 20-fold over that of a conventional imaging lens, liberating the synchronized scanning in LSFM. We captured images of dynamic beating hearts and red blood cells in zebrafish larvae at volume acquisition rates up to 30 Hz.
Conclusions: We demonstrate the fast three-dimensional imaging capability of 2p-LSFM with axicon imaging by recording the rapid dynamics of physiological processes.</abstract><cop>Bellingham</cop><pub>Society of Photo-Optical Instrumentation Engineers</pub><pmid>34796706</pmid><doi>10.1117/1.JBO.26.11.116503</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-6138-711X</orcidid><orcidid>https://orcid.org/0000-0002-1876-7432</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; ProQuest Central |
| subjects | Aperture Biological research Cameras Depth of field Erythrocytes Fluorescence Fluorescence microscopy Image acquisition Larvae Laser microscopy Lasers Lenses Light Light sheets Microscopy Photons Scanning Three dimensional imaging Zebrafish |
| title | Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging |
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