Multiwindow SRS Imaging Using a Rapid Widely Tunable Fiber Laser
Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in a current SRS microscope. Moreover, chemically informa...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2021-11, Vol.93 (47), p.15703-15711 |
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| container_issue | 47 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Ni, Hongli Lin, Peng Zhu, Yifan Zhang, Meng Tan, Yuying Zhan, Yuewei Wang, Zian Cheng, Ji-Xin |
| description | Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in a current SRS microscope. Moreover, chemically informative multiwindow SRS imaging across C–H, C–D, and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multiwindow SRS imaging system based on a compact and robust fiber laser with rapid and wide tuning capability. To address the relative intensity noise intrinsic to a fiber laser, we implemented autobalanced detection, which enhances the signal-to-noise ratio of stimulated Raman loss imaging by 23 times. We demonstrate high-quality SRS metabolic imaging of fungi, cancer cells, and Caenorhabditis elegans across the C–H, C–D, and fingerprint Raman windows. Our results showcase the potential of the compact multiwindow SRS system for a broad range of applications. |
| doi_str_mv | 10.1021/acs.analchem.1c03604 |
| format | Article |
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Yet, wider adoption is hindered by the bulky and environmentally sensitive solid-state optical parametric oscillator (OPO) in a current SRS microscope. Moreover, chemically informative multiwindow SRS imaging across C–H, C–D, and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multiwindow SRS imaging system based on a compact and robust fiber laser with rapid and wide tuning capability. To address the relative intensity noise intrinsic to a fiber laser, we implemented autobalanced detection, which enhances the signal-to-noise ratio of stimulated Raman loss imaging by 23 times. We demonstrate high-quality SRS metabolic imaging of fungi, cancer cells, and Caenorhabditis elegans across the C–H, C–D, and fingerprint Raman windows. 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| subjects | Analytical chemistry Chemistry Diagnostic Tests, Routine Fiber lasers Fingerprints Imaging Lasers Microscopy Noise intensity Optical Parametric Oscillators Parametric amplifiers Raman spectra Relative intensity noise Signal to noise ratio Solid state Spectrum Analysis, Raman Tunable lasers Tuning |
| title | Multiwindow SRS Imaging Using a Rapid Widely Tunable Fiber Laser |
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