Real‐time imaging and developmental biochemistry analysis during embryogenesis of Caridina pseudogracilirostris
This paper reports on the real‐time imaging and developmental biochemistry of the freshwater caridean shrimp, Caridina pseudogracilirostris. The complete time‐lapse development of a single embryo was recorded in an artificial mold, developed in our lab, and imaged under a stereomicroscope. It took 8...
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| Veröffentlicht in: | Journal of experimental zoology. Part A, Ecological and integrative physiology Ecological and integrative physiology, 2022-03, Vol.337 (3), p.206-220 |
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| container_title | Journal of experimental zoology. Part A, Ecological and integrative physiology |
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| creator | Soundharapandiyan, Nandhagopal Thanumalayaperumal, Subramoniam Rajaretinam, Rajesh Kannan |
| description | This paper reports on the real‐time imaging and developmental biochemistry of the freshwater caridean shrimp, Caridina pseudogracilirostris. The complete time‐lapse development of a single embryo was recorded in an artificial mold, developed in our lab, and imaged under a stereomicroscope. It took 8 days to complete the 5 stages of embryonic development (1 cleavage stage, 2 gastrulation stage, 3 nauplius stage, 4 prehatching embryo, and 5 zoea stages). As the decapod eggs are enriched with dense yolk, biochemical determination of the major components was made to evaluate the yolk utilization during embryogenesis. The concentration of protein, lipid, and carbohydrate declined drastically from Stage I (cleavage) to Stage IV (Zoea), reflecting sustained yolk utilization during embryogenesis. The increase in the size of the embryo correlates with changes in water content. Lipids, being the principal organic substrate, changes in the fatty acid (FA) composition of embryos during development were determined by GC. The FA composition was observed within the range of 25%–60.87% for saturated, 22.57%–56.45% for monounsaturated, and 5.64%–18.51% for total polyunsaturated FAs. The essential polyunsaturated fatty acid were higher in Stages I, IV, and V, suggesting a major role in embryogenesis. The cellular proliferation and organogenesis as visualized in the real‐time imaging correlate well with the biochemical variations observed in C. pseudogracilirostris.
Real‐time imaging of isolated embryos under a stereomicroscope using an agarose mold and Biochemical changes during five embryonic developmental stages.
HIGHLIGHTS
Time‐lapse imaging of Caridina pseudogracilirostris embryos during five developmental stages; a new method of in situ embryo culture.
Changes in the fatty acid composition during embryonic development were evaluated.
Real‐time imaging of embryogenesis related to biochemical variation during five stages of development. |
| doi_str_mv | 10.1002/jez.2556 |
| format | Article |
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Real‐time imaging of isolated embryos under a stereomicroscope using an agarose mold and Biochemical changes during five embryonic developmental stages.
HIGHLIGHTS
Time‐lapse imaging of Caridina pseudogracilirostris embryos during five developmental stages; a new method of in situ embryo culture.
Changes in the fatty acid composition during embryonic development were evaluated.
Real‐time imaging of embryogenesis related to biochemical variation during five stages of development.</description><identifier>ISSN: 2471-5638</identifier><identifier>EISSN: 2471-5646</identifier><identifier>DOI: 10.1002/jez.2556</identifier><identifier>PMID: 34783186</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animal embryos ; Animals ; Aquatic crustaceans ; Biochemistry ; Carbohydrates ; caridean embryos ; Caridina ; Caridina pseudogracilirostris ; Cell culture ; Cleavage ; Composition ; Crustaceans ; Decapoda ; developmental imaging ; Developmental stages ; Egg yolk ; Embryogenesis ; Embryonic Development ; Embryonic growth stage ; embryonic stages ; Embryos ; Fatty acid composition ; Fatty acids ; Fatty Acids, Essential ; Freshwater ; Freshwater crustaceans ; Gastrulation ; Imaging ; Imaging techniques ; Inland water environment ; Lipids ; Marine crustaceans ; Moisture content ; Organogenesis ; Polyunsaturated fatty acids ; Proliferation ; Substrates ; Water content ; Yolk ; yolk utilization</subject><ispartof>Journal of experimental zoology. Part A, Ecological and integrative physiology, 2022-03, Vol.337 (3), p.206-220</ispartof><rights>2021 Wiley Periodicals LLC</rights><rights>2021 Wiley Periodicals LLC.</rights><rights>2022 Wiley Periodicals LLC</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3496-2ff5c351e51ce8f4fcc7ba7c7bc09045cf19f1c864961509bed0b2e045a55ac93</citedby><cites>FETCH-LOGICAL-c3496-2ff5c351e51ce8f4fcc7ba7c7bc09045cf19f1c864961509bed0b2e045a55ac93</cites><orcidid>0000-0002-8520-9772 ; 0000-0001-6846-5777</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjez.2556$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjez.2556$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34783186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soundharapandiyan, Nandhagopal</creatorcontrib><creatorcontrib>Thanumalayaperumal, Subramoniam</creatorcontrib><creatorcontrib>Rajaretinam, Rajesh Kannan</creatorcontrib><title>Real‐time imaging and developmental biochemistry analysis during embryogenesis of Caridina pseudogracilirostris</title><title>Journal of experimental zoology. Part A, Ecological and integrative physiology</title><addtitle>J Exp Zool A Ecol Integr Physiol</addtitle><description>This paper reports on the real‐time imaging and developmental biochemistry of the freshwater caridean shrimp, Caridina pseudogracilirostris. The complete time‐lapse development of a single embryo was recorded in an artificial mold, developed in our lab, and imaged under a stereomicroscope. It took 8 days to complete the 5 stages of embryonic development (1 cleavage stage, 2 gastrulation stage, 3 nauplius stage, 4 prehatching embryo, and 5 zoea stages). As the decapod eggs are enriched with dense yolk, biochemical determination of the major components was made to evaluate the yolk utilization during embryogenesis. The concentration of protein, lipid, and carbohydrate declined drastically from Stage I (cleavage) to Stage IV (Zoea), reflecting sustained yolk utilization during embryogenesis. The increase in the size of the embryo correlates with changes in water content. Lipids, being the principal organic substrate, changes in the fatty acid (FA) composition of embryos during development were determined by GC. The FA composition was observed within the range of 25%–60.87% for saturated, 22.57%–56.45% for monounsaturated, and 5.64%–18.51% for total polyunsaturated FAs. The essential polyunsaturated fatty acid were higher in Stages I, IV, and V, suggesting a major role in embryogenesis. The cellular proliferation and organogenesis as visualized in the real‐time imaging correlate well with the biochemical variations observed in C. pseudogracilirostris.
Real‐time imaging of isolated embryos under a stereomicroscope using an agarose mold and Biochemical changes during five embryonic developmental stages.
HIGHLIGHTS
Time‐lapse imaging of Caridina pseudogracilirostris embryos during five developmental stages; a new method of in situ embryo culture.
Changes in the fatty acid composition during embryonic development were evaluated.
Real‐time imaging of embryogenesis related to biochemical variation during five stages of development.</description><subject>Animal embryos</subject><subject>Animals</subject><subject>Aquatic crustaceans</subject><subject>Biochemistry</subject><subject>Carbohydrates</subject><subject>caridean embryos</subject><subject>Caridina</subject><subject>Caridina pseudogracilirostris</subject><subject>Cell culture</subject><subject>Cleavage</subject><subject>Composition</subject><subject>Crustaceans</subject><subject>Decapoda</subject><subject>developmental imaging</subject><subject>Developmental stages</subject><subject>Egg yolk</subject><subject>Embryogenesis</subject><subject>Embryonic Development</subject><subject>Embryonic growth stage</subject><subject>embryonic stages</subject><subject>Embryos</subject><subject>Fatty acid composition</subject><subject>Fatty acids</subject><subject>Fatty Acids, Essential</subject><subject>Freshwater</subject><subject>Freshwater crustaceans</subject><subject>Gastrulation</subject><subject>Imaging</subject><subject>Imaging techniques</subject><subject>Inland water environment</subject><subject>Lipids</subject><subject>Marine crustaceans</subject><subject>Moisture content</subject><subject>Organogenesis</subject><subject>Polyunsaturated fatty acids</subject><subject>Proliferation</subject><subject>Substrates</subject><subject>Water content</subject><subject>Yolk</subject><subject>yolk utilization</subject><issn>2471-5638</issn><issn>2471-5646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9KwzAUh4MoOubAJ5CCN950Jm2Tppcy_MtAEL3xpqTpycxIm5msSr3yEXxGn8RM5wTBmyTkfPnIOT-EDggeE4yTkzm8jhNK2RYaJFlOYsoytr05p3wPjbyfY4wJzyjBbBftpVnOU8LZAD3dgjAfb-9L3UCkGzHT7SwSbR3V8AzGLhpol8JElbbyERrtl64PZWF6r31Ud26FQ1O53s6ghdWlVdFEOF3rVkQLD11tZ05IbbSz4bX2-2hHCeNhtN6H6P787G5yGU9vLq4mp9NYplnB4kQpKlNKgBIJXGVKyrwSeVgkLnBGpSKFIpKzABOKiwpqXCUQKoJSIYt0iI6_vQtnnzrwyzJ8X4IxogXb-TKhBcecEE4DevQHndvOhS4DxdI8KTLC8K9Qhk68A1UuXJiY60uCy1USZUiiXCUR0MO1sKsaqDfgz9wDEH8DL9pA_6-ovD57-BJ-AoVOlSg</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Soundharapandiyan, Nandhagopal</creator><creator>Thanumalayaperumal, Subramoniam</creator><creator>Rajaretinam, Rajesh Kannan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8520-9772</orcidid><orcidid>https://orcid.org/0000-0001-6846-5777</orcidid></search><sort><creationdate>20220301</creationdate><title>Real‐time imaging and developmental biochemistry analysis during embryogenesis of Caridina pseudogracilirostris</title><author>Soundharapandiyan, Nandhagopal ; 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Part A, Ecological and integrative physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soundharapandiyan, Nandhagopal</au><au>Thanumalayaperumal, Subramoniam</au><au>Rajaretinam, Rajesh Kannan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real‐time imaging and developmental biochemistry analysis during embryogenesis of Caridina pseudogracilirostris</atitle><jtitle>Journal of experimental zoology. Part A, Ecological and integrative physiology</jtitle><addtitle>J Exp Zool A Ecol Integr Physiol</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>337</volume><issue>3</issue><spage>206</spage><epage>220</epage><pages>206-220</pages><issn>2471-5638</issn><eissn>2471-5646</eissn><abstract>This paper reports on the real‐time imaging and developmental biochemistry of the freshwater caridean shrimp, Caridina pseudogracilirostris. The complete time‐lapse development of a single embryo was recorded in an artificial mold, developed in our lab, and imaged under a stereomicroscope. It took 8 days to complete the 5 stages of embryonic development (1 cleavage stage, 2 gastrulation stage, 3 nauplius stage, 4 prehatching embryo, and 5 zoea stages). As the decapod eggs are enriched with dense yolk, biochemical determination of the major components was made to evaluate the yolk utilization during embryogenesis. The concentration of protein, lipid, and carbohydrate declined drastically from Stage I (cleavage) to Stage IV (Zoea), reflecting sustained yolk utilization during embryogenesis. The increase in the size of the embryo correlates with changes in water content. Lipids, being the principal organic substrate, changes in the fatty acid (FA) composition of embryos during development were determined by GC. The FA composition was observed within the range of 25%–60.87% for saturated, 22.57%–56.45% for monounsaturated, and 5.64%–18.51% for total polyunsaturated FAs. The essential polyunsaturated fatty acid were higher in Stages I, IV, and V, suggesting a major role in embryogenesis. The cellular proliferation and organogenesis as visualized in the real‐time imaging correlate well with the biochemical variations observed in C. pseudogracilirostris.
Real‐time imaging of isolated embryos under a stereomicroscope using an agarose mold and Biochemical changes during five embryonic developmental stages.
HIGHLIGHTS
Time‐lapse imaging of Caridina pseudogracilirostris embryos during five developmental stages; a new method of in situ embryo culture.
Changes in the fatty acid composition during embryonic development were evaluated.
Real‐time imaging of embryogenesis related to biochemical variation during five stages of development.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34783186</pmid><doi>10.1002/jez.2556</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-8520-9772</orcidid><orcidid>https://orcid.org/0000-0001-6846-5777</orcidid></addata></record> |
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| subjects | Animal embryos Animals Aquatic crustaceans Biochemistry Carbohydrates caridean embryos Caridina Caridina pseudogracilirostris Cell culture Cleavage Composition Crustaceans Decapoda developmental imaging Developmental stages Egg yolk Embryogenesis Embryonic Development Embryonic growth stage embryonic stages Embryos Fatty acid composition Fatty acids Fatty Acids, Essential Freshwater Freshwater crustaceans Gastrulation Imaging Imaging techniques Inland water environment Lipids Marine crustaceans Moisture content Organogenesis Polyunsaturated fatty acids Proliferation Substrates Water content Yolk yolk utilization |
| title | Real‐time imaging and developmental biochemistry analysis during embryogenesis of Caridina pseudogracilirostris |
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