AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening
Ethylene can accelerate the postharvest ripening process of kiwifruit, while indole-3-acetic acid (IAA) delays it. However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, eth...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2021-11, Vol.69 (46), p.13859-13870 |
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| container_title | Journal of agricultural and food chemistry |
| container_volume | 69 |
| creator | Gan, Zengyu Yuan, Xin Shan, Nan Wan, Chunpeng Chen, Chuying Zhu, Liqin Xu, Yunhe Kai, Wenbin Zhai, Xiawan Chen, Jinyin |
| description | Ethylene can accelerate the postharvest ripening process of kiwifruit, while indole-3-acetic acid (IAA) delays it. However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, ethylene can promote the expression of AcGH3.1 and enhance its promoter activity. Two ethylene response factors (ERFs), AcERF1B and AcERF073, were obtained using an AcGH3.1 promoter as bait for a yeast one-hybrid screening library. Both AcERF1B and AcERF073 bind to the AcGH3.1 promoter to activate it. Also, AcERF1B/073 enhanced AcGH3.1 expression, decreased the free IAA content, and increased the IAA-Asp content in kiwifruit. In addition, we found that the AcERF1B and AcERF073 proteins directly interact, and this interaction enhanced their binding to the AcGH3.1 promoter. In summary, our results suggest that AcERF1B and AcERF073 positively regulate IAA degradation by activating AcGH3.1 transcription, which accelerated postharvest kiwifruit ripening. |
| doi_str_mv | 10.1021/acs.jafc.1c03954 |
| format | Article |
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However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, ethylene can promote the expression of AcGH3.1 and enhance its promoter activity. Two ethylene response factors (ERFs), AcERF1B and AcERF073, were obtained using an AcGH3.1 promoter as bait for a yeast one-hybrid screening library. Both AcERF1B and AcERF073 bind to the AcGH3.1 promoter to activate it. Also, AcERF1B/073 enhanced AcGH3.1 expression, decreased the free IAA content, and increased the IAA-Asp content in kiwifruit. In addition, we found that the AcERF1B and AcERF073 proteins directly interact, and this interaction enhanced their binding to the AcGH3.1 promoter. In summary, our results suggest that AcERF1B and AcERF073 positively regulate IAA degradation by activating AcGH3.1 transcription, which accelerated postharvest kiwifruit ripening.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.1c03954</identifier><language>eng</language><publisher>American Chemical Society</publisher><subject>Biotechnology and Biological Transformations</subject><ispartof>Journal of agricultural and food chemistry, 2021-11, Vol.69 (46), p.13859-13870</ispartof><rights>2021 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a313t-6adebfbfa3357159ca3711e7e862f5e69ca11d58cb73ab3f565511727cea14153</citedby><cites>FETCH-LOGICAL-a313t-6adebfbfa3357159ca3711e7e862f5e69ca11d58cb73ab3f565511727cea14153</cites><orcidid>0000-0001-6892-016X ; 0000-0001-7651-1972</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.1c03954$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.1c03954$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids></links><search><creatorcontrib>Gan, Zengyu</creatorcontrib><creatorcontrib>Yuan, Xin</creatorcontrib><creatorcontrib>Shan, Nan</creatorcontrib><creatorcontrib>Wan, Chunpeng</creatorcontrib><creatorcontrib>Chen, Chuying</creatorcontrib><creatorcontrib>Zhu, Liqin</creatorcontrib><creatorcontrib>Xu, Yunhe</creatorcontrib><creatorcontrib>Kai, Wenbin</creatorcontrib><creatorcontrib>Zhai, Xiawan</creatorcontrib><creatorcontrib>Chen, Jinyin</creatorcontrib><title>AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Ethylene can accelerate the postharvest ripening process of kiwifruit, while indole-3-acetic acid (IAA) delays it. However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, ethylene can promote the expression of AcGH3.1 and enhance its promoter activity. Two ethylene response factors (ERFs), AcERF1B and AcERF073, were obtained using an AcGH3.1 promoter as bait for a yeast one-hybrid screening library. Both AcERF1B and AcERF073 bind to the AcGH3.1 promoter to activate it. Also, AcERF1B/073 enhanced AcGH3.1 expression, decreased the free IAA content, and increased the IAA-Asp content in kiwifruit. In addition, we found that the AcERF1B and AcERF073 proteins directly interact, and this interaction enhanced their binding to the AcGH3.1 promoter. In summary, our results suggest that AcERF1B and AcERF073 positively regulate IAA degradation by activating AcGH3.1 transcription, which accelerated postharvest kiwifruit ripening.</description><subject>Biotechnology and Biological Transformations</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kE9PwzAMxSMEEmNw55gjB1rihjTdccAGiEmgCc6Vm7ojqLQlaUH7HHxhsj9XTnH8fs-yH2PnIGIQCVyh8fEHViYGI-REXR-wEahERAogO2QjEZgoUykcsxPvP4QQmdJixH6nZracww3HpuTbWmjJX1pve_tN9ZovaTXU2BN_bMq2pkhGaKi3JsC25He0clhib9uGF-vQC67wa1ahvH-QMfBXh403znZbphzcRgzz-3d03-R7_mR_bOUG2_Ol7agJ8ik7qrD2dLZ_x-xtPnu9fYgWz_ePt9NFhBJkH6VYUlEVFUqpNKiJQakBSFOWJpWiNDQASpWZQkssZKVSFcLQiTaEcA1KjtnFbm7n2q8h7JJ_Wm-orrGhdvB5oiY6C4ZMB1TsUONa7x1VeefsJ7p1DiLf5J-H_PNN_vk-_2C53Fm2Sju4JtzyP_4H1bWJjQ</recordid><startdate>20211124</startdate><enddate>20211124</enddate><creator>Gan, Zengyu</creator><creator>Yuan, Xin</creator><creator>Shan, Nan</creator><creator>Wan, Chunpeng</creator><creator>Chen, Chuying</creator><creator>Zhu, Liqin</creator><creator>Xu, Yunhe</creator><creator>Kai, Wenbin</creator><creator>Zhai, Xiawan</creator><creator>Chen, Jinyin</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6892-016X</orcidid><orcidid>https://orcid.org/0000-0001-7651-1972</orcidid></search><sort><creationdate>20211124</creationdate><title>AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening</title><author>Gan, Zengyu ; Yuan, Xin ; Shan, Nan ; Wan, Chunpeng ; Chen, Chuying ; Zhu, Liqin ; Xu, Yunhe ; Kai, Wenbin ; Zhai, Xiawan ; Chen, Jinyin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a313t-6adebfbfa3357159ca3711e7e862f5e69ca11d58cb73ab3f565511727cea14153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biotechnology and Biological Transformations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gan, Zengyu</creatorcontrib><creatorcontrib>Yuan, Xin</creatorcontrib><creatorcontrib>Shan, Nan</creatorcontrib><creatorcontrib>Wan, Chunpeng</creatorcontrib><creatorcontrib>Chen, Chuying</creatorcontrib><creatorcontrib>Zhu, Liqin</creatorcontrib><creatorcontrib>Xu, Yunhe</creatorcontrib><creatorcontrib>Kai, Wenbin</creatorcontrib><creatorcontrib>Zhai, Xiawan</creatorcontrib><creatorcontrib>Chen, Jinyin</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gan, Zengyu</au><au>Yuan, Xin</au><au>Shan, Nan</au><au>Wan, Chunpeng</au><au>Chen, Chuying</au><au>Zhu, Liqin</au><au>Xu, Yunhe</au><au>Kai, Wenbin</au><au>Zhai, Xiawan</au><au>Chen, Jinyin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2021-11-24</date><risdate>2021</risdate><volume>69</volume><issue>46</issue><spage>13859</spage><epage>13870</epage><pages>13859-13870</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Ethylene can accelerate the postharvest ripening process of kiwifruit, while indole-3-acetic acid (IAA) delays it. However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, ethylene can promote the expression of AcGH3.1 and enhance its promoter activity. Two ethylene response factors (ERFs), AcERF1B and AcERF073, were obtained using an AcGH3.1 promoter as bait for a yeast one-hybrid screening library. Both AcERF1B and AcERF073 bind to the AcGH3.1 promoter to activate it. Also, AcERF1B/073 enhanced AcGH3.1 expression, decreased the free IAA content, and increased the IAA-Asp content in kiwifruit. In addition, we found that the AcERF1B and AcERF073 proteins directly interact, and this interaction enhanced their binding to the AcGH3.1 promoter. In summary, our results suggest that AcERF1B and AcERF073 positively regulate IAA degradation by activating AcGH3.1 transcription, which accelerated postharvest kiwifruit ripening.</abstract><pub>American Chemical Society</pub><doi>10.1021/acs.jafc.1c03954</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6892-016X</orcidid><orcidid>https://orcid.org/0000-0001-7651-1972</orcidid></addata></record> |
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| title | AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening |
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