Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates
The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S. aureus isolates (including 25 human, 25 raw milk and 20 pet animal isolates) were subjected to the antimi...
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| Veröffentlicht in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2021-12, Vol.79, p.101717-101717, Article 101717 |
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| creator | Pahlavanzadeh, Sara Khoshbakht, Rahem Kaboosi, Hami Moazamian, Elham |
| description | The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S. aureus isolates (including 25 human, 25 raw milk and 20 pet animal isolates) were subjected to the antimicrobial susceptibility testing, polymerase chain reaction (PCR) detection of the resistance genes and DNA fingerprinting using random amplification of polymorphic DNA–PCR (RAPD-PCR) to survey the variability of the isolates. Among 70 S. aureus, 55 (78.5%) isolates were MRSA. The isolates showed the highest antibiotic resistance to methicillin, ampicillin and penicillin (78.5%) and showed the lowest resistance to ciprofloxacin (12.8%). ErmB and tetM resistance genes were present in all isolates and the vanA gene was not detected in any of the isolates. Thirteen distinct clusters were identified in RAPD-PCR fingerprinting. Statistical analysis showed that the isolates without resistance to antibiotics were significantly in associated with raw milk origin (P |
| doi_str_mv | 10.1016/j.cimid.2021.101717 |
| format | Article |
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•High occurrence rate of MDR S. aureus strains was detected among isolates.•High occurrence rate of MRSA strains was distinguished among S. aureus isolates.•The VRSA strains and the vanB gene were not detected among S. aureus isolates.•Nonresistant S. aureus isolates were significantly in associated with raw milk.•RAPD-PCR showed a high ability to generate a distinct pattern for various isolates.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2021.101717</identifier><identifier>PMID: 34763201</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Ampicillin ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotic resistance ; Antibiotics ; Antiinfectives and antibacterials ; Ciprofloxacin ; Deoxyribonucleic acid ; DNA ; DNA fingerprinting ; Drug resistance ; Drug Resistance, Microbial ; Genes ; Genetic fingerprinting ; Genotyping ; Humans ; Infections ; Methicillin ; Methicillin-Resistant Staphylococcus aureus - genetics ; Microbial Sensitivity Tests - veterinary ; Milk ; MRSA ; Penicillin ; Pets ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Random amplified polymorphic DNA ; Random Amplified Polymorphic DNA Technique - veterinary ; RAPD-PCR ; Resistance genes ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus infections ; Statistical analysis ; VanA gene</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2021-12, Vol.79, p.101717-101717, Article 101717</ispartof><rights>2021 Elsevier Ltd</rights><rights>Copyright © 2021 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Dec 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-6a1887ba31a3e314f22c060fe832c5c142bc0ef8dc41e936952e53b7532cd9e13</citedby><cites>FETCH-LOGICAL-c387t-6a1887ba31a3e314f22c060fe832c5c142bc0ef8dc41e936952e53b7532cd9e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2021.101717$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34763201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pahlavanzadeh, Sara</creatorcontrib><creatorcontrib>Khoshbakht, Rahem</creatorcontrib><creatorcontrib>Kaboosi, Hami</creatorcontrib><creatorcontrib>Moazamian, Elham</creatorcontrib><title>Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S. aureus isolates (including 25 human, 25 raw milk and 20 pet animal isolates) were subjected to the antimicrobial susceptibility testing, polymerase chain reaction (PCR) detection of the resistance genes and DNA fingerprinting using random amplification of polymorphic DNA–PCR (RAPD-PCR) to survey the variability of the isolates. Among 70 S. aureus, 55 (78.5%) isolates were MRSA. The isolates showed the highest antibiotic resistance to methicillin, ampicillin and penicillin (78.5%) and showed the lowest resistance to ciprofloxacin (12.8%). ErmB and tetM resistance genes were present in all isolates and the vanA gene was not detected in any of the isolates. Thirteen distinct clusters were identified in RAPD-PCR fingerprinting. Statistical analysis showed that the isolates without resistance to antibiotics were significantly in associated with raw milk origin (P < 0.05). According to the results of the study, S. aureus strains with pets and raw milk origin are significant sources of antibiotic-resistant isolates such as MRSA. They are also carriers of resistance genes that can be transmit to human isolates and cause drug resistance in human infections. Identifying the source of these infections is possible with a reliable genotyping method such as RAPD-PCR.
•High occurrence rate of MDR S. aureus strains was detected among isolates.•High occurrence rate of MRSA strains was distinguished among S. aureus isolates.•The VRSA strains and the vanB gene were not detected among S. aureus isolates.•Nonresistant S. aureus isolates were significantly in associated with raw milk.•RAPD-PCR showed a high ability to generate a distinct pattern for various isolates.</description><subject>Ampicillin</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Ciprofloxacin</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA fingerprinting</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial</subject><subject>Genes</subject><subject>Genetic fingerprinting</subject><subject>Genotyping</subject><subject>Humans</subject><subject>Infections</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus - genetics</subject><subject>Microbial Sensitivity Tests - veterinary</subject><subject>Milk</subject><subject>MRSA</subject><subject>Penicillin</subject><subject>Pets</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Random amplified polymorphic DNA</subject><subject>Random Amplified Polymorphic DNA Technique - veterinary</subject><subject>RAPD-PCR</subject><subject>Resistance genes</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus infections</subject><subject>Statistical analysis</subject><subject>VanA gene</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFO3DAQhi1UVBbaJ6hUReqFQ7N4bCd2DhwQorQSEgfo2XKcCXibxMF2inh7vLu0hx56GsnzfeOxf0I-AV0Dhfpss7ZudN2aUQbbEwnygKxASVVCXct3ZEVByLKpJByR4xg3lNIGBLwnR1zImjMKKxIvpuRa55OzRcDoYjKTxcJMXTE_vgz-ASfc9qwfZxNc9FPh--JxGc30tZgxZdKNZog7I5jnYnTDr-IumZ1tvbVL7i0Bc8n2YBLGD-Swzwp-fKsn5Oe3q_vL7-XN7fWPy4ub0nIlU1kbUEq2hoPhyEH0jFla0x4VZ7ayIFhrKfaqswKw4XVTMax4K6vc7hoEfkJO93Pn4J8WjEmPLlocBjOhX6JmVSNFo6qGZ_TLP-jGL2HK22lWMy4qKZTIFN9TNvgYA_Z6Dvn14UUD1dtM9EbvMtHbTPQ-k2x9fpu9tCN2f50_IWTgfA9g_ozfDoOO1mGOoXMBbdKdd_-94BUd-J7Q</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Pahlavanzadeh, Sara</creator><creator>Khoshbakht, Rahem</creator><creator>Kaboosi, Hami</creator><creator>Moazamian, Elham</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>202112</creationdate><title>Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates</title><author>Pahlavanzadeh, Sara ; Khoshbakht, Rahem ; Kaboosi, Hami ; Moazamian, Elham</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-6a1887ba31a3e314f22c060fe832c5c142bc0ef8dc41e936952e53b7532cd9e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Ampicillin</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Ciprofloxacin</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA fingerprinting</topic><topic>Drug resistance</topic><topic>Drug Resistance, Microbial</topic><topic>Genes</topic><topic>Genetic fingerprinting</topic><topic>Genotyping</topic><topic>Humans</topic><topic>Infections</topic><topic>Methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus - genetics</topic><topic>Microbial Sensitivity Tests - veterinary</topic><topic>Milk</topic><topic>MRSA</topic><topic>Penicillin</topic><topic>Pets</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Random amplified polymorphic DNA</topic><topic>Random Amplified Polymorphic DNA Technique - veterinary</topic><topic>RAPD-PCR</topic><topic>Resistance genes</topic><topic>Staphylococcal Infections - veterinary</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus infections</topic><topic>Statistical analysis</topic><topic>VanA gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pahlavanzadeh, Sara</creatorcontrib><creatorcontrib>Khoshbakht, Rahem</creatorcontrib><creatorcontrib>Kaboosi, Hami</creatorcontrib><creatorcontrib>Moazamian, Elham</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pahlavanzadeh, Sara</au><au>Khoshbakht, Rahem</au><au>Kaboosi, Hami</au><au>Moazamian, Elham</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2021-12</date><risdate>2021</risdate><volume>79</volume><spage>101717</spage><epage>101717</epage><pages>101717-101717</pages><artnum>101717</artnum><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>The present study was conducted to compare the S. aureus isolates from different sources in the basis of resistance phenotypic and genotypic features and phylogenetic differences. Total of 70 S. aureus isolates (including 25 human, 25 raw milk and 20 pet animal isolates) were subjected to the antimicrobial susceptibility testing, polymerase chain reaction (PCR) detection of the resistance genes and DNA fingerprinting using random amplification of polymorphic DNA–PCR (RAPD-PCR) to survey the variability of the isolates. Among 70 S. aureus, 55 (78.5%) isolates were MRSA. The isolates showed the highest antibiotic resistance to methicillin, ampicillin and penicillin (78.5%) and showed the lowest resistance to ciprofloxacin (12.8%). ErmB and tetM resistance genes were present in all isolates and the vanA gene was not detected in any of the isolates. Thirteen distinct clusters were identified in RAPD-PCR fingerprinting. Statistical analysis showed that the isolates without resistance to antibiotics were significantly in associated with raw milk origin (P < 0.05). According to the results of the study, S. aureus strains with pets and raw milk origin are significant sources of antibiotic-resistant isolates such as MRSA. They are also carriers of resistance genes that can be transmit to human isolates and cause drug resistance in human infections. Identifying the source of these infections is possible with a reliable genotyping method such as RAPD-PCR.
•High occurrence rate of MDR S. aureus strains was detected among isolates.•High occurrence rate of MRSA strains was distinguished among S. aureus isolates.•The VRSA strains and the vanB gene were not detected among S. aureus isolates.•Nonresistant S. aureus isolates were significantly in associated with raw milk.•RAPD-PCR showed a high ability to generate a distinct pattern for various isolates.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>34763201</pmid><doi>10.1016/j.cimid.2021.101717</doi><tpages>1</tpages></addata></record> |
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| ispartof | Comparative immunology, microbiology and infectious diseases, 2021-12, Vol.79, p.101717-101717, Article 101717 |
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| subjects | Ampicillin Animals Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antiinfectives and antibacterials Ciprofloxacin Deoxyribonucleic acid DNA DNA fingerprinting Drug resistance Drug Resistance, Microbial Genes Genetic fingerprinting Genotyping Humans Infections Methicillin Methicillin-Resistant Staphylococcus aureus - genetics Microbial Sensitivity Tests - veterinary Milk MRSA Penicillin Pets Phylogenetics Phylogeny Polymerase chain reaction Random amplified polymorphic DNA Random Amplified Polymorphic DNA Technique - veterinary RAPD-PCR Resistance genes Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus infections Statistical analysis VanA gene |
| title | Antibiotic resistance and phylogenetic comparison of human, pet animals and raw milk Staphylococcus aureus isolates |
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