Adaptive laboratory evolution for improved tolerance of isobutyl acetate in Escherichia coli

Previously, Escherichia coli was engineered to produce isobutyl acetate (IBA). Titers greater than the toxicity threshold (3 g/L) were achieved by using layer-assisted production. To avoid this costly and complex method, adaptive laboratory evolution (ALE) was applied to E. coli for improved IBA tol...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Metabolic engineering 2022-01, Vol.69, p.50-58
Hauptverfasser:	Matson, Morgan M., Cepeda, Mateo M., Zhang, Angela, Case, Anna E., Kavvas, Erol S., Wang, Xiaokang, Carroll, Austin L., Tagkopoulos, Ilias, Atsumi, Shota
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates Adaptive laboratory evolution Escherichia coli - genetics Escherichia coli Proteins - genetics Esters Genome sequencing Laboratories Metabolic engineering Tolerance
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!