RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis
Key message The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms. Cassava ( Manihot esculenta ) is a tropical root crop affected by different patho...
Gespeichert in:
| Veröffentlicht in: | Plant molecular biology 2022-06, Vol.109 (3), p.313-324 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 324 |
|---|---|
| container_issue | 3 |
| container_start_page | 313 |
| container_title | Plant molecular biology |
| container_volume | 109 |
| creator | Díaz-Tatis, Paula A. Ochoa, Juan C. Rico, Edgar M. Rodríguez, Catalina Medina, Adriana Szurek, Boris Chavarriaga, Paul López, Camilo E. |
| description | Key message
The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms.
Cassava (
Manihot esculenta
) is a tropical root crop affected by different pathogens including
Xanthomonas phaseoli
pv. manihotis (
Xpm
), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to
Xpm
strain CIO151 called
RXam2.
Transgenic cassava plants overexpressing
RXam2
were generated and analyzed. Plants overexpressing
RXam2
showed a reduction in bacterial growth to
Xpm
strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the
RXam2
promoter region. The
Xpm
strain transformed with dTALEs allowed the induction of the
RXam2
gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that
RXam2
contributes to the understanding of the molecular basis of quantitative disease resistance. |
| doi_str_mv | 10.1007/s11103-021-01211-2 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2596022466</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A705890958</galeid><sourcerecordid>A705890958</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-95f7661cf264c93bb1f1ead3bb3c8ddf9be9b2dd14607ab3ab0ec7ef33717e443</originalsourceid><addsrcrecordid>eNp9kc9u1DAQxiMEokvhBTggS1yKRBaP7cSbY1WVP9ICUgVSb9bEmXRdJfHWdlbqG_DYeEkBiQPywdbM75v55K8oXgJfA-f6XQQALksuoOQgAErxqFhBpWVZcbF5XKw41LpUCsRJ8SzGW86zTNZPixOpdKUraFbFj6trHMVbhuzL9or1wY_MYox4QHb2GSe384lRtPNAU8I3zPopBdfOiSLbY0gOh-GeJc_SjtjdjFNyCZM7EAsUXUw4WTq2r3Nn50c_YdbtMJIfHNsf1mxcdrj4vHjS4xDpxcN9Wnx_f_nt4mO5_frh08X5trQKVCqbqtd1DbYXtbKNbFvogbDLD2k3Xdc3LTWt6DpQNdfYSmw5WU29lBo0KSVPi7Nl7j74u5liMqOLloYBJ_JzNKJqai6EquuMvv4HvfVzmLI7I2otVCOBi0ytF-oGBzJu6n0KaPPpaHT5v6h3uX6uebVpeFNtskAsAht8jIF6sw9uxHBvgJtjsGYJ1uRgza9gzXHLqwcvcztS90fyO8kMyAWIuTXdUPhr9j9jfwJV369c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2672493102</pqid></control><display><type>article</type><title>RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Díaz-Tatis, Paula A. ; Ochoa, Juan C. ; Rico, Edgar M. ; Rodríguez, Catalina ; Medina, Adriana ; Szurek, Boris ; Chavarriaga, Paul ; López, Camilo E.</creator><creatorcontrib>Díaz-Tatis, Paula A. ; Ochoa, Juan C. ; Rico, Edgar M. ; Rodríguez, Catalina ; Medina, Adriana ; Szurek, Boris ; Chavarriaga, Paul ; López, Camilo E.</creatorcontrib><description>Key message
The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms.
Cassava (
Manihot esculenta
) is a tropical root crop affected by different pathogens including
Xanthomonas phaseoli
pv. manihotis (
Xpm
), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to
Xpm
strain CIO151 called
RXam2.
Transgenic cassava plants overexpressing
RXam2
were generated and analyzed. Plants overexpressing
RXam2
showed a reduction in bacterial growth to
Xpm
strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the
RXam2
promoter region. The
Xpm
strain transformed with dTALEs allowed the induction of the
RXam2
gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that
RXam2
contributes to the understanding of the molecular basis of quantitative disease resistance.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/s11103-021-01211-2</identifier><identifier>PMID: 34757519</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Bacteria ; Biochemistry ; Biomedical and Life Sciences ; Blight ; Cassava ; Cassava Molecular Biology ; Disease resistance ; Gene expression ; Genes ; Genetic engineering ; Genetic transformation ; Inoculation ; Leucine ; Life Sciences ; Manihot - genetics ; Manihot esculenta ; Nucleotides ; Plant Diseases - microbiology ; Plant Pathology ; Plant Sciences ; Polygenic inheritance ; Quantitative genetics ; Quantitative trait loci ; Transgenic plants ; Xanthomonas ; Xanthomonas phaseoli</subject><ispartof>Plant molecular biology, 2022-06, Vol.109 (3), p.313-324</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-95f7661cf264c93bb1f1ead3bb3c8ddf9be9b2dd14607ab3ab0ec7ef33717e443</citedby><cites>FETCH-LOGICAL-c414t-95f7661cf264c93bb1f1ead3bb3c8ddf9be9b2dd14607ab3ab0ec7ef33717e443</cites><orcidid>0000-0003-4592-8614</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11103-021-01211-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11103-021-01211-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34757519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Díaz-Tatis, Paula A.</creatorcontrib><creatorcontrib>Ochoa, Juan C.</creatorcontrib><creatorcontrib>Rico, Edgar M.</creatorcontrib><creatorcontrib>Rodríguez, Catalina</creatorcontrib><creatorcontrib>Medina, Adriana</creatorcontrib><creatorcontrib>Szurek, Boris</creatorcontrib><creatorcontrib>Chavarriaga, Paul</creatorcontrib><creatorcontrib>López, Camilo E.</creatorcontrib><title>RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><addtitle>Plant Mol Biol</addtitle><description>Key message
The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms.
Cassava (
Manihot esculenta
) is a tropical root crop affected by different pathogens including
Xanthomonas phaseoli
pv. manihotis (
Xpm
), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to
Xpm
strain CIO151 called
RXam2.
Transgenic cassava plants overexpressing
RXam2
were generated and analyzed. Plants overexpressing
RXam2
showed a reduction in bacterial growth to
Xpm
strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the
RXam2
promoter region. The
Xpm
strain transformed with dTALEs allowed the induction of the
RXam2
gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that
RXam2
contributes to the understanding of the molecular basis of quantitative disease resistance.</description><subject>Analysis</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Cassava</subject><subject>Cassava Molecular Biology</subject><subject>Disease resistance</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic transformation</subject><subject>Inoculation</subject><subject>Leucine</subject><subject>Life Sciences</subject><subject>Manihot - genetics</subject><subject>Manihot esculenta</subject><subject>Nucleotides</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Polygenic inheritance</subject><subject>Quantitative genetics</subject><subject>Quantitative trait loci</subject><subject>Transgenic plants</subject><subject>Xanthomonas</subject><subject>Xanthomonas phaseoli</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kc9u1DAQxiMEokvhBTggS1yKRBaP7cSbY1WVP9ICUgVSb9bEmXRdJfHWdlbqG_DYeEkBiQPywdbM75v55K8oXgJfA-f6XQQALksuoOQgAErxqFhBpWVZcbF5XKw41LpUCsRJ8SzGW86zTNZPixOpdKUraFbFj6trHMVbhuzL9or1wY_MYox4QHb2GSe384lRtPNAU8I3zPopBdfOiSLbY0gOh-GeJc_SjtjdjFNyCZM7EAsUXUw4WTq2r3Nn50c_YdbtMJIfHNsf1mxcdrj4vHjS4xDpxcN9Wnx_f_nt4mO5_frh08X5trQKVCqbqtd1DbYXtbKNbFvogbDLD2k3Xdc3LTWt6DpQNdfYSmw5WU29lBo0KSVPi7Nl7j74u5liMqOLloYBJ_JzNKJqai6EquuMvv4HvfVzmLI7I2otVCOBi0ytF-oGBzJu6n0KaPPpaHT5v6h3uX6uebVpeFNtskAsAht8jIF6sw9uxHBvgJtjsGYJ1uRgza9gzXHLqwcvcztS90fyO8kMyAWIuTXdUPhr9j9jfwJV369c</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Díaz-Tatis, Paula A.</creator><creator>Ochoa, Juan C.</creator><creator>Rico, Edgar M.</creator><creator>Rodríguez, Catalina</creator><creator>Medina, Adriana</creator><creator>Szurek, Boris</creator><creator>Chavarriaga, Paul</creator><creator>López, Camilo E.</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4592-8614</orcidid></search><sort><creationdate>20220601</creationdate><title>RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis</title><author>Díaz-Tatis, Paula A. ; Ochoa, Juan C. ; Rico, Edgar M. ; Rodríguez, Catalina ; Medina, Adriana ; Szurek, Boris ; Chavarriaga, Paul ; López, Camilo E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-95f7661cf264c93bb1f1ead3bb3c8ddf9be9b2dd14607ab3ab0ec7ef33717e443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Cassava</topic><topic>Cassava Molecular Biology</topic><topic>Disease resistance</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic transformation</topic><topic>Inoculation</topic><topic>Leucine</topic><topic>Life Sciences</topic><topic>Manihot - genetics</topic><topic>Manihot esculenta</topic><topic>Nucleotides</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Polygenic inheritance</topic><topic>Quantitative genetics</topic><topic>Quantitative trait loci</topic><topic>Transgenic plants</topic><topic>Xanthomonas</topic><topic>Xanthomonas phaseoli</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Díaz-Tatis, Paula A.</creatorcontrib><creatorcontrib>Ochoa, Juan C.</creatorcontrib><creatorcontrib>Rico, Edgar M.</creatorcontrib><creatorcontrib>Rodríguez, Catalina</creatorcontrib><creatorcontrib>Medina, Adriana</creatorcontrib><creatorcontrib>Szurek, Boris</creatorcontrib><creatorcontrib>Chavarriaga, Paul</creatorcontrib><creatorcontrib>López, Camilo E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Díaz-Tatis, Paula A.</au><au>Ochoa, Juan C.</au><au>Rico, Edgar M.</au><au>Rodríguez, Catalina</au><au>Medina, Adriana</au><au>Szurek, Boris</au><au>Chavarriaga, Paul</au><au>López, Camilo E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis</atitle><jtitle>Plant molecular biology</jtitle><stitle>Plant Mol Biol</stitle><addtitle>Plant Mol Biol</addtitle><date>2022-06-01</date><risdate>2022</risdate><volume>109</volume><issue>3</issue><spage>313</spage><epage>324</epage><pages>313-324</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Key message
The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms.
Cassava (
Manihot esculenta
) is a tropical root crop affected by different pathogens including
Xanthomonas phaseoli
pv. manihotis (
Xpm
), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to
Xpm
strain CIO151 called
RXam2.
Transgenic cassava plants overexpressing
RXam2
were generated and analyzed. Plants overexpressing
RXam2
showed a reduction in bacterial growth to
Xpm
strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the
RXam2
promoter region. The
Xpm
strain transformed with dTALEs allowed the induction of the
RXam2
gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that
RXam2
contributes to the understanding of the molecular basis of quantitative disease resistance.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34757519</pmid><doi>10.1007/s11103-021-01211-2</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4592-8614</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 2022-06, Vol.109 (3), p.313-324 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2596022466 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Analysis Bacteria Biochemistry Biomedical and Life Sciences Blight Cassava Cassava Molecular Biology Disease resistance Gene expression Genes Genetic engineering Genetic transformation Inoculation Leucine Life Sciences Manihot - genetics Manihot esculenta Nucleotides Plant Diseases - microbiology Plant Pathology Plant Sciences Polygenic inheritance Quantitative genetics Quantitative trait loci Transgenic plants Xanthomonas Xanthomonas phaseoli |
| title | RXam2, a NLR from cassava (Manihot esculenta) contributes partially to the quantitative resistance to Xanthomonas phaseoli pv. manihotis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T09%3A44%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RXam2,%20a%20NLR%20from%20cassava%20(Manihot%20esculenta)%20contributes%20partially%20to%20the%20quantitative%20resistance%20to%20Xanthomonas%20phaseoli%20pv.%20manihotis&rft.jtitle=Plant%20molecular%20biology&rft.au=D%C3%ADaz-Tatis,%20Paula%20A.&rft.date=2022-06-01&rft.volume=109&rft.issue=3&rft.spage=313&rft.epage=324&rft.pages=313-324&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1007/s11103-021-01211-2&rft_dat=%3Cgale_proqu%3EA705890958%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2672493102&rft_id=info:pmid/34757519&rft_galeid=A705890958&rfr_iscdi=true |