Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)

Dispersal flights by the mountain pine beetle have allowed range expansion and major damage to pine stands in western Canada. We asked what the genetic and transcriptional basis of mountain pine beetle dispersal capacity is. Using flight mills, RNA-seq and a targeted association study, we compared s...

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Veröffentlicht in:PeerJ (San Francisco, CA) CA), 2021-10, Vol.9, p.e12382-e12382, Article 12382
Hauptverfasser: Shegelski, Victor A., Evenden, Maya L., Huber, Dezene P. W., Sperling, Felix A. H.
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Sperling, Felix A. H.
description Dispersal flights by the mountain pine beetle have allowed range expansion and major damage to pine stands in western Canada. We asked what the genetic and transcriptional basis of mountain pine beetle dispersal capacity is. Using flight mills, RNA-seq and a targeted association study, we compared strong-flying, weak-flying, and non-flying female beetles from the recently colonized northern end of their range. Nearly 3,000 genes were differentially expressed between strong and weak flying beetles, while weak fliers and nonfliers did not significantly differ. The differentially expressed genes were mainly associated with lipid metabolism, muscle maintenance, oxidative stress response, detoxification, endocrine function, and flight behavior. Three variant loci, two in the coding region of genes, were significantly associated with flight capacity but these genes had no known functional link to flight. Several differentially expressed gene systems may be important for sustained flight, while other systems are downregulated during dispersal and likely to conserve energy before host colonization. The candidate genes and SNPs identified here will inform further studies and management of mountain pine beetle, as well as contribute to understanding the mechanisms of insect dispersal flights.
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W.</au><au>Sperling, Felix A. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)</atitle><jtitle>PeerJ (San Francisco, CA)</jtitle><stitle>PEERJ</stitle><date>2021-10-26</date><risdate>2021</risdate><volume>9</volume><spage>e12382</spage><epage>e12382</epage><pages>e12382-e12382</pages><artnum>12382</artnum><artnum>e12382</artnum><issn>2167-8359</issn><eissn>2167-8359</eissn><abstract>Dispersal flights by the mountain pine beetle have allowed range expansion and major damage to pine stands in western Canada. We asked what the genetic and transcriptional basis of mountain pine beetle dispersal capacity is. Using flight mills, RNA-seq and a targeted association study, we compared strong-flying, weak-flying, and non-flying female beetles from the recently colonized northern end of their range. Nearly 3,000 genes were differentially expressed between strong and weak flying beetles, while weak fliers and nonfliers did not significantly differ. The differentially expressed genes were mainly associated with lipid metabolism, muscle maintenance, oxidative stress response, detoxification, endocrine function, and flight behavior. Three variant loci, two in the coding region of genes, were significantly associated with flight capacity but these genes had no known functional link to flight. Several differentially expressed gene systems may be important for sustained flight, while other systems are downregulated during dispersal and likely to conserve energy before host colonization. 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subjects Bark beetles
Bioassays
Canada
Cellular stress response
Coleoptera
Dendroctonus ponderosae
Detoxification
Dispersal
Energy conservation
Entomology
Females
Flight behavior
Flight genetics
Flight mill
Forest pest
Gene expression
Genes
Genetic aspects
Genetic systems
Genetic transcription
Genomes
Genomics
Insects
Lipid metabolism
Metabolism
Molecular Biology
Morphology
Multidisciplinary Sciences
Oxidative metabolism
Oxidative stress
Physiological aspects
Range extension
RNA
Science & Technology
Science & Technology - Other Topics
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Transcription
Zoology
title Identification of genes and gene expression associated with dispersal capacity in the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae)
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