Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms
We describe an efficient method to condense RNAs into tightly packed RNA nanoparticles (RNPs) for biomedical applications without hydrophobic or cationic agents. We embedded kissing loops and siRNA in the RNAs to constrain the size of RNPs to 100 nm, making them suitable not only for cellular uptake...
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| Veröffentlicht in: | Biomaterials science 2021-12, Vol.9 (24), p.8148-8152 |
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| container_title | Biomaterials science |
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| creator | Kim, Kyoung-Ran Kim, Junghyun Mao, Chengde Ahn, Dae-Ro |
| description | We describe an efficient method to condense RNAs into tightly packed RNA nanoparticles (RNPs) for biomedical applications without hydrophobic or cationic agents. We embedded kissing loops and siRNA in the RNAs to constrain the size of RNPs to
100 nm, making them suitable not only for cellular uptake but also for passive tumor accumulation. The resulting RNPs were efficiently internalized into cells and downregulated the target gene of siRNAs. When intravenously injected into tumor-bearing mice, RNPs could also accumulate in the tumor. The reported fabrication method could be readily adopted as a platform to prepare RNPs for
and
delivery of bioactive RNAs. |
| doi_str_mv | 10.1039/d1bm01440d |
| format | Article |
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100 nm, making them suitable not only for cellular uptake but also for passive tumor accumulation. The resulting RNPs were efficiently internalized into cells and downregulated the target gene of siRNAs. When intravenously injected into tumor-bearing mice, RNPs could also accumulate in the tumor. The reported fabrication method could be readily adopted as a platform to prepare RNPs for
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delivery of bioactive RNAs.</description><identifier>ISSN: 2047-4830</identifier><identifier>EISSN: 2047-4849</identifier><identifier>DOI: 10.1039/d1bm01440d</identifier><identifier>PMID: 34755728</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Biomedical materials ; Cations ; Cell Line, Tumor ; In vivo methods and tests ; Mice ; Nanoparticles ; Ribonucleic acid ; RNA ; RNA, Small Interfering - genetics ; Tumors</subject><ispartof>Biomaterials science, 2021-12, Vol.9 (24), p.8148-8152</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-980acad82b0015e61a93a39b08b9c2c2cb256b42e67148158b5f4843d01fd3733</citedby><cites>FETCH-LOGICAL-c315t-980acad82b0015e61a93a39b08b9c2c2cb256b42e67148158b5f4843d01fd3733</cites><orcidid>0000-0001-7516-8666 ; 0000-0001-7108-5855</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34755728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Kyoung-Ran</creatorcontrib><creatorcontrib>Kim, Junghyun</creatorcontrib><creatorcontrib>Mao, Chengde</creatorcontrib><creatorcontrib>Ahn, Dae-Ro</creatorcontrib><title>Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms</title><title>Biomaterials science</title><addtitle>Biomater Sci</addtitle><description>We describe an efficient method to condense RNAs into tightly packed RNA nanoparticles (RNPs) for biomedical applications without hydrophobic or cationic agents. We embedded kissing loops and siRNA in the RNAs to constrain the size of RNPs to
100 nm, making them suitable not only for cellular uptake but also for passive tumor accumulation. The resulting RNPs were efficiently internalized into cells and downregulated the target gene of siRNAs. When intravenously injected into tumor-bearing mice, RNPs could also accumulate in the tumor. The reported fabrication method could be readily adopted as a platform to prepare RNPs for
and
delivery of bioactive RNAs.</description><subject>Animals</subject><subject>Biomedical materials</subject><subject>Cations</subject><subject>Cell Line, Tumor</subject><subject>In vivo methods and tests</subject><subject>Mice</subject><subject>Nanoparticles</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Small Interfering - genetics</subject><subject>Tumors</subject><issn>2047-4830</issn><issn>2047-4849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1LHjEQx0OpVFEv_QAl0EsprOZtN5uj1aqlvkBpz8tkk20j2WSbZB8Qv7x51HrozGEG5jd_hvkj9J6SI0q4OjZUz4QKQcwbtMeIkI3ohXr72nOyiw5zviM1pFSko-_QLheybSXr99DDd5ezC7-xj3FpZmscFGvwBDq5EYqLAccJ_7g5wQFCXCAVN3qbMQSDyx_rEl5isaE48BgyHq33q4f0NHcBb9wm4uy2-8Z6t7HpHi8eyhTTnA_QzgQ-28OXuo9-nX_9eXrZXN1efDs9uWpGTtvSqJ7ACKZnmhDa2o6C4sCVJr1WI6upWdtpwWwnqehp2-t2qj_ghtDJcMn5Pvr0rLuk-He1uQyzy9tLIdi45oG1qqvKpJcV_fgfehfXFOp1A-uI5JIpISr1-ZkaU8w52WlYkpsh3Q-UDFtXhjP65frJlbMKf3iRXHX97yv6zwP-CIRihxM</recordid><startdate>20211207</startdate><enddate>20211207</enddate><creator>Kim, Kyoung-Ran</creator><creator>Kim, Junghyun</creator><creator>Mao, Chengde</creator><creator>Ahn, Dae-Ro</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7516-8666</orcidid><orcidid>https://orcid.org/0000-0001-7108-5855</orcidid></search><sort><creationdate>20211207</creationdate><title>Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms</title><author>Kim, Kyoung-Ran ; Kim, Junghyun ; Mao, Chengde ; Ahn, Dae-Ro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-980acad82b0015e61a93a39b08b9c2c2cb256b42e67148158b5f4843d01fd3733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Biomedical materials</topic><topic>Cations</topic><topic>Cell Line, Tumor</topic><topic>In vivo methods and tests</topic><topic>Mice</topic><topic>Nanoparticles</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Small Interfering - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Kyoung-Ran</creatorcontrib><creatorcontrib>Kim, Junghyun</creatorcontrib><creatorcontrib>Mao, Chengde</creatorcontrib><creatorcontrib>Ahn, Dae-Ro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Kyoung-Ran</au><au>Kim, Junghyun</au><au>Mao, Chengde</au><au>Ahn, Dae-Ro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms</atitle><jtitle>Biomaterials science</jtitle><addtitle>Biomater Sci</addtitle><date>2021-12-07</date><risdate>2021</risdate><volume>9</volume><issue>24</issue><spage>8148</spage><epage>8152</epage><pages>8148-8152</pages><issn>2047-4830</issn><eissn>2047-4849</eissn><abstract>We describe an efficient method to condense RNAs into tightly packed RNA nanoparticles (RNPs) for biomedical applications without hydrophobic or cationic agents. We embedded kissing loops and siRNA in the RNAs to constrain the size of RNPs to
100 nm, making them suitable not only for cellular uptake but also for passive tumor accumulation. The resulting RNPs were efficiently internalized into cells and downregulated the target gene of siRNAs. When intravenously injected into tumor-bearing mice, RNPs could also accumulate in the tumor. The reported fabrication method could be readily adopted as a platform to prepare RNPs for
and
delivery of bioactive RNAs.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>34755728</pmid><doi>10.1039/d1bm01440d</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-7516-8666</orcidid><orcidid>https://orcid.org/0000-0001-7108-5855</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2047-4830 |
| ispartof | Biomaterials science, 2021-12, Vol.9 (24), p.8148-8152 |
| issn | 2047-4830 2047-4849 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Animals Biomedical materials Cations Cell Line, Tumor In vivo methods and tests Mice Nanoparticles Ribonucleic acid RNA RNA, Small Interfering - genetics Tumors |
| title | Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms |
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