An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags
A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nu...
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| Veröffentlicht in: | Analytica chimica acta 2021-12, Vol.1187, p.339155-339155, Article 339155 |
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| container_title | Analytica chimica acta |
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| creator | Cheng, Siyun Tu, Zhijie Zheng, Shuai Cheng, Xiaodan Han, Han Wang, Chongwen Xiao, Rui Gu, Bing |
| description | A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nucleic acid aptamers. WGA was modified onto Fe3O4@Au magnetic nanoparticles (MNPs) for the efficient capture of S. aureus and L. mono in complex samples (orange juice, extracts of lettuce, and human urine) within 15 min. The streptavidin (SA)/aptamers co-functionalized SERS tags were fabricated by covalent attaching two different Raman reporters and SA molecules onto 45 nm Au NPs and then conjugated with two biotin-aptamers that specifically bind to their target bacteria with high affinity and stability. The combined use of high-sensitive SERS tags, WGA-mediated magnetic enrichment, and SA-mediated aptamer conjugation remarkably improved the assay sensitivity. Under optimized conditions, the developed SERS biosensor can simultaneously detect the two target bacteria with high detection sensitivity ( |
| doi_str_mv | 10.1016/j.aca.2021.339155 |
| format | Article |
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[Display omitted]
•A rapid and ultrasensitive SERS platform for S. aureus and L. mono detection was reported.•WGA modified-Fe3O4@Au MNP was proposed for broad-spectrum capture of multiple bacteria.•The SA/aptamer co-functionalized SERS tags exhibited higher affinity and stability.•The LODs for S. aureus and L. mono were 3 and 5 cells/mL, respectively.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2021.339155</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Dual-recognition ; Multiplex detection ; Pathogenic bacteria ; SERS ; WGA</subject><ispartof>Analytica chimica acta, 2021-12, Vol.1187, p.339155-339155, Article 339155</ispartof><rights>2021 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-cbbcf8d4246bffa640016bfb586394e848784c534c7a8ee48fad53062e0dc5953</citedby><cites>FETCH-LOGICAL-c330t-cbbcf8d4246bffa640016bfb586394e848784c534c7a8ee48fad53062e0dc5953</cites><orcidid>0000-0002-9627-107X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267021009818$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Cheng, Siyun</creatorcontrib><creatorcontrib>Tu, Zhijie</creatorcontrib><creatorcontrib>Zheng, Shuai</creatorcontrib><creatorcontrib>Cheng, Xiaodan</creatorcontrib><creatorcontrib>Han, Han</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><creatorcontrib>Xiao, Rui</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><title>An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags</title><title>Analytica chimica acta</title><description>A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nucleic acid aptamers. WGA was modified onto Fe3O4@Au magnetic nanoparticles (MNPs) for the efficient capture of S. aureus and L. mono in complex samples (orange juice, extracts of lettuce, and human urine) within 15 min. The streptavidin (SA)/aptamers co-functionalized SERS tags were fabricated by covalent attaching two different Raman reporters and SA molecules onto 45 nm Au NPs and then conjugated with two biotin-aptamers that specifically bind to their target bacteria with high affinity and stability. The combined use of high-sensitive SERS tags, WGA-mediated magnetic enrichment, and SA-mediated aptamer conjugation remarkably improved the assay sensitivity. Under optimized conditions, the developed SERS biosensor can simultaneously detect the two target bacteria with high detection sensitivity (<6 cells/mL), favorable linear relation (10-107 cells/mL), and high accuracy (recovery rate <7.03%). Therefore, the proposed SERS platform is rapid, sensitive, easy to use, and thus show potential as a tool for the timely identification of pathogenic bacteria in real samples.
[Display omitted]
•A rapid and ultrasensitive SERS platform for S. aureus and L. mono detection was reported.•WGA modified-Fe3O4@Au MNP was proposed for broad-spectrum capture of multiple bacteria.•The SA/aptamer co-functionalized SERS tags exhibited higher affinity and stability.•The LODs for S. aureus and L. mono were 3 and 5 cells/mL, respectively.</description><subject>Dual-recognition</subject><subject>Multiplex detection</subject><subject>Pathogenic bacteria</subject><subject>SERS</subject><subject>WGA</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kcGO0zAQhiMEEmXhAbj5yCVdO3bSVJxWq2VBqoRE4WxN7HHqKrGD7RSVl-VVcBrOXDwz1vz_jP0VxXtGt4yy5v68BQXbilZsy_me1fWLYsPaHS8Fr8TLYkMp5WXV7Ojr4k2M51xWjIpN8efBETTGKosukePTtyOZBkjGh5Hkg6QTknlIASK6aJO9INGYUCXrHfGGHBNMp-vglVdqjgTmgEtwmhxsTBgskNE7r67J9-gwkku--XVCSKTHPAP6fpiTddaVo9fWWNRkhN5hsmpdJ85dzPMT3lxzilOCi9XW3UPORgxE-dLM7rYTDPZ3trgpE_TxbfHKwBDx3b94V_z49PT98XN5-Pr85fHhUCrOaSpV1ynTalGJpjMGGkHzp3amq9uG7wW2ot21QtVcqB20iKI1oGtOmwqpVvW-5nfFh9V3Cv7njDHJ0UaFwwAO_RxlVe8bygRrlla2tqrgYwxo5BTsCOEqGZULTHmWGaZcYMoVZtZ8XDWY33CxGGRciCnUNmQYUnv7H_Vffz2t6g</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Cheng, Siyun</creator><creator>Tu, Zhijie</creator><creator>Zheng, Shuai</creator><creator>Cheng, Xiaodan</creator><creator>Han, Han</creator><creator>Wang, Chongwen</creator><creator>Xiao, Rui</creator><creator>Gu, Bing</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9627-107X</orcidid></search><sort><creationdate>20211201</creationdate><title>An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags</title><author>Cheng, Siyun ; Tu, Zhijie ; Zheng, Shuai ; Cheng, Xiaodan ; Han, Han ; Wang, Chongwen ; Xiao, Rui ; Gu, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-cbbcf8d4246bffa640016bfb586394e848784c534c7a8ee48fad53062e0dc5953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Dual-recognition</topic><topic>Multiplex detection</topic><topic>Pathogenic bacteria</topic><topic>SERS</topic><topic>WGA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Siyun</creatorcontrib><creatorcontrib>Tu, Zhijie</creatorcontrib><creatorcontrib>Zheng, Shuai</creatorcontrib><creatorcontrib>Cheng, Xiaodan</creatorcontrib><creatorcontrib>Han, Han</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><creatorcontrib>Xiao, Rui</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Siyun</au><au>Tu, Zhijie</au><au>Zheng, Shuai</au><au>Cheng, Xiaodan</au><au>Han, Han</au><au>Wang, Chongwen</au><au>Xiao, Rui</au><au>Gu, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags</atitle><jtitle>Analytica chimica acta</jtitle><date>2021-12-01</date><risdate>2021</risdate><volume>1187</volume><spage>339155</spage><epage>339155</epage><pages>339155-339155</pages><artnum>339155</artnum><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nucleic acid aptamers. WGA was modified onto Fe3O4@Au magnetic nanoparticles (MNPs) for the efficient capture of S. aureus and L. mono in complex samples (orange juice, extracts of lettuce, and human urine) within 15 min. The streptavidin (SA)/aptamers co-functionalized SERS tags were fabricated by covalent attaching two different Raman reporters and SA molecules onto 45 nm Au NPs and then conjugated with two biotin-aptamers that specifically bind to their target bacteria with high affinity and stability. The combined use of high-sensitive SERS tags, WGA-mediated magnetic enrichment, and SA-mediated aptamer conjugation remarkably improved the assay sensitivity. Under optimized conditions, the developed SERS biosensor can simultaneously detect the two target bacteria with high detection sensitivity (<6 cells/mL), favorable linear relation (10-107 cells/mL), and high accuracy (recovery rate <7.03%). Therefore, the proposed SERS platform is rapid, sensitive, easy to use, and thus show potential as a tool for the timely identification of pathogenic bacteria in real samples.
[Display omitted]
•A rapid and ultrasensitive SERS platform for S. aureus and L. mono detection was reported.•WGA modified-Fe3O4@Au MNP was proposed for broad-spectrum capture of multiple bacteria.•The SA/aptamer co-functionalized SERS tags exhibited higher affinity and stability.•The LODs for S. aureus and L. mono were 3 and 5 cells/mL, respectively.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.aca.2021.339155</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9627-107X</orcidid></addata></record> |
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| subjects | Dual-recognition Multiplex detection Pathogenic bacteria SERS WGA |
| title | An efficient SERS platform for the ultrasensitive detection of Staphylococcus aureus and Listeria monocytogenes via wheat germ agglutinin-modified magnetic SERS substrate and streptavidin/aptamer co-functionalized SERS tags |
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