Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples
Aims Escherichia albertii is an emerging diarrheagenic pathogen causing food‐ and water‐borne infection in humans. However, no selective enrichment broths for E. albertii have ever been reported. In this study, we tested several basal media, selective supplements and culture conditions which enabled...
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| Veröffentlicht in: | Journal of applied microbiology 2022-03, Vol.132 (3), p.2121-2130 |
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| container_title | Journal of applied microbiology |
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| creator | Wakabayashi, Yuki Seto, Kazuko Kanki, Masashi Harada, Tetsuya Kawatsu, Kentaro |
| description | Aims
Escherichia albertii is an emerging diarrheagenic pathogen causing food‐ and water‐borne infection in humans. However, no selective enrichment broths for E. albertii have ever been reported. In this study, we tested several basal media, selective supplements and culture conditions which enabled selective enrichment of E. albertii.
Methods and Results
We developed a selective enrichment broth, novobiocin–cefixime–tellurite supplemented modified tryptic soy broth (NCT‐mTSB). NCT‐mTSB supported the growth of 22 E. albertii strains, while inhibited growth of other Enterobacteriaceae at 37°C, except for Escherichia coli and Shigella spp. Enrichment of E. albertii was improved further by growth at 44°C, a temperature that suppresses growth of several strains of E. coli/Shigella. Combined use of NCT‐mTSB with XR‐DH‐agar, xylose–rhamnose supplemented deoxycholate hydrogen sulphide agar, enabled isolation of E. albertii when at least 1 CFU of the bacterium was present per gram of chicken meat. This level of enrichment was superior to those obtained using buffered peptone water, modified‐EC broth, or mTSB (with novobiocin).
Conclusions
Novobiocin–cefixime–tellurite supplemented modified tryptic soy broth enabled effective enrichment of E. albertii from poultry samples and was helpful for isolation of this bacterium.
Significance and Impact of Study
To our knowledge, this is the first report of selective enrichment of E. albertii from poultry samples. |
| doi_str_mv | 10.1111/jam.15353 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2594294009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2630849482</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4193-3a3626cb4d8e4196ba0ac0f9e19fc715e86eb047cffdcacacbaea076c5282b803</originalsourceid><addsrcrecordid>eNp1kctu1DAUhi0EoqWw4AWQJTYgNa3vSZZlVNqicpEY1pbjOdZ45MTBTgbNjkfgGXmSejqFBRLHi3N09PmT5R-hl5Sc0VLnG9OfUcklf4SOKVeyYqpmj-9nUUlSsyP0LOcNIZQTqZ6iIy5qLmtJjtH2S4pjzCbg6LDBQ9xCwBkC2MlvAcOQvF33MEy4S3Fan-JPi-Xvn7_65dd3p9jFhH2OwUw-DnvBZbZr2N_wBpvQQZq8xy7FHo9xDlPa4Wz6MUB-jp44EzK8eOgn6Nv7y-Xiurr9fHWzuLitrKAtr7jhiinbiVUDZaE6Q4wlrgXaOltTCY2CjojaOreyppzOgCG1spI1rGsIP0FvDt4xxe8z5En3PlsIwQwQ56yZbAVrBSFtQV__g27inIbyOs0UJ41oRcMK9fZA2RRzTuD0mHxv0k5Tovdh6BKGvg-jsK8ejHPXw-ov-ef3C3B-AH74ALv_m_SHi48H5R3PWZVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2630849482</pqid></control><display><type>article</type><title>Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Wakabayashi, Yuki ; Seto, Kazuko ; Kanki, Masashi ; Harada, Tetsuya ; Kawatsu, Kentaro</creator><creatorcontrib>Wakabayashi, Yuki ; Seto, Kazuko ; Kanki, Masashi ; Harada, Tetsuya ; Kawatsu, Kentaro</creatorcontrib><description>Aims
Escherichia albertii is an emerging diarrheagenic pathogen causing food‐ and water‐borne infection in humans. However, no selective enrichment broths for E. albertii have ever been reported. In this study, we tested several basal media, selective supplements and culture conditions which enabled selective enrichment of E. albertii.
Methods and Results
We developed a selective enrichment broth, novobiocin–cefixime–tellurite supplemented modified tryptic soy broth (NCT‐mTSB). NCT‐mTSB supported the growth of 22 E. albertii strains, while inhibited growth of other Enterobacteriaceae at 37°C, except for Escherichia coli and Shigella spp. Enrichment of E. albertii was improved further by growth at 44°C, a temperature that suppresses growth of several strains of E. coli/Shigella. Combined use of NCT‐mTSB with XR‐DH‐agar, xylose–rhamnose supplemented deoxycholate hydrogen sulphide agar, enabled isolation of E. albertii when at least 1 CFU of the bacterium was present per gram of chicken meat. This level of enrichment was superior to those obtained using buffered peptone water, modified‐EC broth, or mTSB (with novobiocin).
Conclusions
Novobiocin–cefixime–tellurite supplemented modified tryptic soy broth enabled effective enrichment of E. albertii from poultry samples and was helpful for isolation of this bacterium.
Significance and Impact of Study
To our knowledge, this is the first report of selective enrichment of E. albertii from poultry samples.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15353</identifier><identifier>PMID: 34735750</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Bacteria ; Broths ; Caseins ; Cefixime ; Culture Media ; E coli ; Enrichment ; Escherichia - isolation & purification ; Escherichia albertii ; Food Microbiology ; food safety ; Hydrogen sulfide ; isolation method ; Meat ; Novobiocin ; Novobiocin - pharmacology ; Peptones ; Poultry ; Poultry - microbiology ; poultry meat ; Protein Hydrolysates ; Rhamnose ; selective enrichment ; Selective media ; Shigella ; Tellurite ; Tellurium ; Tellurium dioxide</subject><ispartof>Journal of applied microbiology, 2022-03, Vol.132 (3), p.2121-2130</ispartof><rights>2021 The Society for Applied Microbiology</rights><rights>2021 The Society for Applied Microbiology.</rights><rights>Copyright © 2022 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4193-3a3626cb4d8e4196ba0ac0f9e19fc715e86eb047cffdcacacbaea076c5282b803</citedby><cites>FETCH-LOGICAL-c4193-3a3626cb4d8e4196ba0ac0f9e19fc715e86eb047cffdcacacbaea076c5282b803</cites><orcidid>0000-0001-9786-9121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15353$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15353$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34735750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakabayashi, Yuki</creatorcontrib><creatorcontrib>Seto, Kazuko</creatorcontrib><creatorcontrib>Kanki, Masashi</creatorcontrib><creatorcontrib>Harada, Tetsuya</creatorcontrib><creatorcontrib>Kawatsu, Kentaro</creatorcontrib><title>Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Escherichia albertii is an emerging diarrheagenic pathogen causing food‐ and water‐borne infection in humans. However, no selective enrichment broths for E. albertii have ever been reported. In this study, we tested several basal media, selective supplements and culture conditions which enabled selective enrichment of E. albertii.
Methods and Results
We developed a selective enrichment broth, novobiocin–cefixime–tellurite supplemented modified tryptic soy broth (NCT‐mTSB). NCT‐mTSB supported the growth of 22 E. albertii strains, while inhibited growth of other Enterobacteriaceae at 37°C, except for Escherichia coli and Shigella spp. Enrichment of E. albertii was improved further by growth at 44°C, a temperature that suppresses growth of several strains of E. coli/Shigella. Combined use of NCT‐mTSB with XR‐DH‐agar, xylose–rhamnose supplemented deoxycholate hydrogen sulphide agar, enabled isolation of E. albertii when at least 1 CFU of the bacterium was present per gram of chicken meat. This level of enrichment was superior to those obtained using buffered peptone water, modified‐EC broth, or mTSB (with novobiocin).
Conclusions
Novobiocin–cefixime–tellurite supplemented modified tryptic soy broth enabled effective enrichment of E. albertii from poultry samples and was helpful for isolation of this bacterium.
Significance and Impact of Study
To our knowledge, this is the first report of selective enrichment of E. albertii from poultry samples.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Broths</subject><subject>Caseins</subject><subject>Cefixime</subject><subject>Culture Media</subject><subject>E coli</subject><subject>Enrichment</subject><subject>Escherichia - isolation & purification</subject><subject>Escherichia albertii</subject><subject>Food Microbiology</subject><subject>food safety</subject><subject>Hydrogen sulfide</subject><subject>isolation method</subject><subject>Meat</subject><subject>Novobiocin</subject><subject>Novobiocin - pharmacology</subject><subject>Peptones</subject><subject>Poultry</subject><subject>Poultry - microbiology</subject><subject>poultry meat</subject><subject>Protein Hydrolysates</subject><subject>Rhamnose</subject><subject>selective enrichment</subject><subject>Selective media</subject><subject>Shigella</subject><subject>Tellurite</subject><subject>Tellurium</subject><subject>Tellurium dioxide</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctu1DAUhi0EoqWw4AWQJTYgNa3vSZZlVNqicpEY1pbjOdZ45MTBTgbNjkfgGXmSejqFBRLHi3N09PmT5R-hl5Sc0VLnG9OfUcklf4SOKVeyYqpmj-9nUUlSsyP0LOcNIZQTqZ6iIy5qLmtJjtH2S4pjzCbg6LDBQ9xCwBkC2MlvAcOQvF33MEy4S3Fan-JPi-Xvn7_65dd3p9jFhH2OwUw-DnvBZbZr2N_wBpvQQZq8xy7FHo9xDlPa4Wz6MUB-jp44EzK8eOgn6Nv7y-Xiurr9fHWzuLitrKAtr7jhiinbiVUDZaE6Q4wlrgXaOltTCY2CjojaOreyppzOgCG1spI1rGsIP0FvDt4xxe8z5En3PlsIwQwQ56yZbAVrBSFtQV__g27inIbyOs0UJ41oRcMK9fZA2RRzTuD0mHxv0k5Tovdh6BKGvg-jsK8ejHPXw-ov-ef3C3B-AH74ALv_m_SHi48H5R3PWZVQ</recordid><startdate>202203</startdate><enddate>202203</enddate><creator>Wakabayashi, Yuki</creator><creator>Seto, Kazuko</creator><creator>Kanki, Masashi</creator><creator>Harada, Tetsuya</creator><creator>Kawatsu, Kentaro</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9786-9121</orcidid></search><sort><creationdate>202203</creationdate><title>Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples</title><author>Wakabayashi, Yuki ; Seto, Kazuko ; Kanki, Masashi ; Harada, Tetsuya ; Kawatsu, Kentaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4193-3a3626cb4d8e4196ba0ac0f9e19fc715e86eb047cffdcacacbaea076c5282b803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Broths</topic><topic>Caseins</topic><topic>Cefixime</topic><topic>Culture Media</topic><topic>E coli</topic><topic>Enrichment</topic><topic>Escherichia - isolation & purification</topic><topic>Escherichia albertii</topic><topic>Food Microbiology</topic><topic>food safety</topic><topic>Hydrogen sulfide</topic><topic>isolation method</topic><topic>Meat</topic><topic>Novobiocin</topic><topic>Novobiocin - pharmacology</topic><topic>Peptones</topic><topic>Poultry</topic><topic>Poultry - microbiology</topic><topic>poultry meat</topic><topic>Protein Hydrolysates</topic><topic>Rhamnose</topic><topic>selective enrichment</topic><topic>Selective media</topic><topic>Shigella</topic><topic>Tellurite</topic><topic>Tellurium</topic><topic>Tellurium dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakabayashi, Yuki</creatorcontrib><creatorcontrib>Seto, Kazuko</creatorcontrib><creatorcontrib>Kanki, Masashi</creatorcontrib><creatorcontrib>Harada, Tetsuya</creatorcontrib><creatorcontrib>Kawatsu, Kentaro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakabayashi, Yuki</au><au>Seto, Kazuko</au><au>Kanki, Masashi</au><au>Harada, Tetsuya</au><au>Kawatsu, Kentaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2022-03</date><risdate>2022</risdate><volume>132</volume><issue>3</issue><spage>2121</spage><epage>2130</epage><pages>2121-2130</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Escherichia albertii is an emerging diarrheagenic pathogen causing food‐ and water‐borne infection in humans. However, no selective enrichment broths for E. albertii have ever been reported. In this study, we tested several basal media, selective supplements and culture conditions which enabled selective enrichment of E. albertii.
Methods and Results
We developed a selective enrichment broth, novobiocin–cefixime–tellurite supplemented modified tryptic soy broth (NCT‐mTSB). NCT‐mTSB supported the growth of 22 E. albertii strains, while inhibited growth of other Enterobacteriaceae at 37°C, except for Escherichia coli and Shigella spp. Enrichment of E. albertii was improved further by growth at 44°C, a temperature that suppresses growth of several strains of E. coli/Shigella. Combined use of NCT‐mTSB with XR‐DH‐agar, xylose–rhamnose supplemented deoxycholate hydrogen sulphide agar, enabled isolation of E. albertii when at least 1 CFU of the bacterium was present per gram of chicken meat. This level of enrichment was superior to those obtained using buffered peptone water, modified‐EC broth, or mTSB (with novobiocin).
Conclusions
Novobiocin–cefixime–tellurite supplemented modified tryptic soy broth enabled effective enrichment of E. albertii from poultry samples and was helpful for isolation of this bacterium.
Significance and Impact of Study
To our knowledge, this is the first report of selective enrichment of E. albertii from poultry samples.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>34735750</pmid><doi>10.1111/jam.15353</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9786-9121</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2022-03, Vol.132 (3), p.2121-2130 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Bacteria Broths Caseins Cefixime Culture Media E coli Enrichment Escherichia - isolation & purification Escherichia albertii Food Microbiology food safety Hydrogen sulfide isolation method Meat Novobiocin Novobiocin - pharmacology Peptones Poultry Poultry - microbiology poultry meat Protein Hydrolysates Rhamnose selective enrichment Selective media Shigella Tellurite Tellurium Tellurium dioxide |
| title | Proposal of a novel selective enrichment broth, NCT‐mTSB, for isolation of Escherichia albertii from poultry samples |
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