Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study
Background Although dysbiosis and the role of the microbiome in the pathogenesis of inflammatory skin diseases have been intensively investigated, fungal colonization or infection has received minimal attention. Aims To isolate and identify different fungal species namely Candida, Dermatophytes, Mal...
Gespeichert in:
| Veröffentlicht in: | Journal of cosmetic dermatology 2022-07, Vol.21 (7), p.3059-3067 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3067 |
|---|---|
| container_issue | 7 |
| container_start_page | 3059 |
| container_title | Journal of cosmetic dermatology |
| container_volume | 21 |
| creator | Sanad, Eman M. K. Nazmy, Nader N. Abd‐El Hamid El Sayed, Rasha Hamed, Ahmed M. |
| description | Background
Although dysbiosis and the role of the microbiome in the pathogenesis of inflammatory skin diseases have been intensively investigated, fungal colonization or infection has received minimal attention.
Aims
To isolate and identify different fungal species namely Candida, Dermatophytes, Malassezia, and Aspergillus from plaque psoriasis patients, evaluate the association of IL‐17A gene single nucleotide polymorphisms (SNPs) with psoriasis, and to reveal the relation between IL‐17A gene SNPs and the fungal presence within the psoriatic plaques.
Patients/Methods
Fifty plaque psoriasis patients and fifty healthy age and sex volunteers as controls were enrolled in this study. From psoriatic plaques, mycological isolation was done by direct microscopic examination (10% KOH mount), culture onto the three sets of media then species identification by phenotypic procedures. Genomic DNA extraction and genotyping for IL‐17A (rs10484879) SNPs using polymerase chain reaction and restriction fragment length polymorphism were also done.
Results
Psoriasis cases showed higher frequency of fungal growth 86% vs. 14% in controls; (p |
| doi_str_mv | 10.1111/jocd.14551 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2594290666</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2594290666</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3061-f3513be7fa365d6463401e854a5a551fbd180f19246d3ff6791934eab7c84c43</originalsourceid><addsrcrecordid>eNp9kM1OAjEQxzdGExG9-AQ9GhOwpR_LeiP4hSHhwn1TdqdQ7LZr243hZnwCn9EnsYjx6BxmJpnfTOb_z7JLgockxc3WVfWQMM7JUdYjLKeDERH8-K-n-DQ7C2GLMckLwnvZx8xG8Aa6F22_3j9JPkFrsICCtmsDyHaVARd1Dah1Ztc43250aJC0NdIxIA9GRu0sig6pzq6lQWvv3uIGaYva4LxO4wq1KYON4RZNUJt2dKOt9DsUYlfvzrMTJU2Ai9_az5YP98vp02C-eJxNJ_NBRbEgA0U5oSvIlaSC14IJyjCBMWeSy6RXrWoyxooUIyZqqpRI-grKQK7yaswqRvvZ1eFs691rByGWjQ4VGCMtuC6UI16wUYGFEAm9PqCVdyF4UGXrdZMeLgku9z6Xe5_LH58TTA7wmzaw-4csnxfTu8PON0V_gxY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2594290666</pqid></control><display><type>article</type><title>Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study</title><source>Wiley Journals</source><creator>Sanad, Eman M. K. ; Nazmy, Nader N. ; Abd‐El Hamid El Sayed, Rasha ; Hamed, Ahmed M.</creator><creatorcontrib>Sanad, Eman M. K. ; Nazmy, Nader N. ; Abd‐El Hamid El Sayed, Rasha ; Hamed, Ahmed M.</creatorcontrib><description>Background
Although dysbiosis and the role of the microbiome in the pathogenesis of inflammatory skin diseases have been intensively investigated, fungal colonization or infection has received minimal attention.
Aims
To isolate and identify different fungal species namely Candida, Dermatophytes, Malassezia, and Aspergillus from plaque psoriasis patients, evaluate the association of IL‐17A gene single nucleotide polymorphisms (SNPs) with psoriasis, and to reveal the relation between IL‐17A gene SNPs and the fungal presence within the psoriatic plaques.
Patients/Methods
Fifty plaque psoriasis patients and fifty healthy age and sex volunteers as controls were enrolled in this study. From psoriatic plaques, mycological isolation was done by direct microscopic examination (10% KOH mount), culture onto the three sets of media then species identification by phenotypic procedures. Genomic DNA extraction and genotyping for IL‐17A (rs10484879) SNPs using polymerase chain reaction and restriction fragment length polymorphism were also done.
Results
Psoriasis cases showed higher frequency of fungal growth 86% vs. 14% in controls; (p < 0.001). The frequency of IL‐17A GA, AA, and total polymorphism (GA+AA) genotypes in psoriasis cases was significantly higher than in controls. There was non‐significant association between different IL‐17A genotypes and fungal growth except Aspergillus flavus, which decreased gradually with GG, GA, and AA (37.5%, 20.8%, and 0%, respectively).
Conclusions
Psoriasis cases are significantly associated with fungal growth, which may be a contributing factor in its pathogenesis. SNPs of IL‐17A (rs10484879) G/A gene led to increased susceptibility toward pathogenesis of psoriasis. Fungal growth and IL‐17A GA+AA genotypes are suggested to be independent predictors of psoriasis susceptibility.</description><identifier>ISSN: 1473-2130</identifier><identifier>EISSN: 1473-2165</identifier><identifier>DOI: 10.1111/jocd.14551</identifier><language>eng</language><subject>culture ; fungal infections ; IL‐17A ; psoriasis ; single nucleotide polymorphism</subject><ispartof>Journal of cosmetic dermatology, 2022-07, Vol.21 (7), p.3059-3067</ispartof><rights>2021 Wiley Periodicals LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3061-f3513be7fa365d6463401e854a5a551fbd180f19246d3ff6791934eab7c84c43</citedby><cites>FETCH-LOGICAL-c3061-f3513be7fa365d6463401e854a5a551fbd180f19246d3ff6791934eab7c84c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjocd.14551$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjocd.14551$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Sanad, Eman M. K.</creatorcontrib><creatorcontrib>Nazmy, Nader N.</creatorcontrib><creatorcontrib>Abd‐El Hamid El Sayed, Rasha</creatorcontrib><creatorcontrib>Hamed, Ahmed M.</creatorcontrib><title>Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study</title><title>Journal of cosmetic dermatology</title><description>Background
Although dysbiosis and the role of the microbiome in the pathogenesis of inflammatory skin diseases have been intensively investigated, fungal colonization or infection has received minimal attention.
Aims
To isolate and identify different fungal species namely Candida, Dermatophytes, Malassezia, and Aspergillus from plaque psoriasis patients, evaluate the association of IL‐17A gene single nucleotide polymorphisms (SNPs) with psoriasis, and to reveal the relation between IL‐17A gene SNPs and the fungal presence within the psoriatic plaques.
Patients/Methods
Fifty plaque psoriasis patients and fifty healthy age and sex volunteers as controls were enrolled in this study. From psoriatic plaques, mycological isolation was done by direct microscopic examination (10% KOH mount), culture onto the three sets of media then species identification by phenotypic procedures. Genomic DNA extraction and genotyping for IL‐17A (rs10484879) SNPs using polymerase chain reaction and restriction fragment length polymorphism were also done.
Results
Psoriasis cases showed higher frequency of fungal growth 86% vs. 14% in controls; (p < 0.001). The frequency of IL‐17A GA, AA, and total polymorphism (GA+AA) genotypes in psoriasis cases was significantly higher than in controls. There was non‐significant association between different IL‐17A genotypes and fungal growth except Aspergillus flavus, which decreased gradually with GG, GA, and AA (37.5%, 20.8%, and 0%, respectively).
Conclusions
Psoriasis cases are significantly associated with fungal growth, which may be a contributing factor in its pathogenesis. SNPs of IL‐17A (rs10484879) G/A gene led to increased susceptibility toward pathogenesis of psoriasis. Fungal growth and IL‐17A GA+AA genotypes are suggested to be independent predictors of psoriasis susceptibility.</description><subject>culture</subject><subject>fungal infections</subject><subject>IL‐17A</subject><subject>psoriasis</subject><subject>single nucleotide polymorphism</subject><issn>1473-2130</issn><issn>1473-2165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OAjEQxzdGExG9-AQ9GhOwpR_LeiP4hSHhwn1TdqdQ7LZr243hZnwCn9EnsYjx6BxmJpnfTOb_z7JLgockxc3WVfWQMM7JUdYjLKeDERH8-K-n-DQ7C2GLMckLwnvZx8xG8Aa6F22_3j9JPkFrsICCtmsDyHaVARd1Dah1Ztc43250aJC0NdIxIA9GRu0sig6pzq6lQWvv3uIGaYva4LxO4wq1KYON4RZNUJt2dKOt9DsUYlfvzrMTJU2Ai9_az5YP98vp02C-eJxNJ_NBRbEgA0U5oSvIlaSC14IJyjCBMWeSy6RXrWoyxooUIyZqqpRI-grKQK7yaswqRvvZ1eFs691rByGWjQ4VGCMtuC6UI16wUYGFEAm9PqCVdyF4UGXrdZMeLgku9z6Xe5_LH58TTA7wmzaw-4csnxfTu8PON0V_gxY</recordid><startdate>202207</startdate><enddate>202207</enddate><creator>Sanad, Eman M. K.</creator><creator>Nazmy, Nader N.</creator><creator>Abd‐El Hamid El Sayed, Rasha</creator><creator>Hamed, Ahmed M.</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202207</creationdate><title>Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study</title><author>Sanad, Eman M. K. ; Nazmy, Nader N. ; Abd‐El Hamid El Sayed, Rasha ; Hamed, Ahmed M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3061-f3513be7fa365d6463401e854a5a551fbd180f19246d3ff6791934eab7c84c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>culture</topic><topic>fungal infections</topic><topic>IL‐17A</topic><topic>psoriasis</topic><topic>single nucleotide polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanad, Eman M. K.</creatorcontrib><creatorcontrib>Nazmy, Nader N.</creatorcontrib><creatorcontrib>Abd‐El Hamid El Sayed, Rasha</creatorcontrib><creatorcontrib>Hamed, Ahmed M.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cosmetic dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanad, Eman M. K.</au><au>Nazmy, Nader N.</au><au>Abd‐El Hamid El Sayed, Rasha</au><au>Hamed, Ahmed M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study</atitle><jtitle>Journal of cosmetic dermatology</jtitle><date>2022-07</date><risdate>2022</risdate><volume>21</volume><issue>7</issue><spage>3059</spage><epage>3067</epage><pages>3059-3067</pages><issn>1473-2130</issn><eissn>1473-2165</eissn><abstract>Background
Although dysbiosis and the role of the microbiome in the pathogenesis of inflammatory skin diseases have been intensively investigated, fungal colonization or infection has received minimal attention.
Aims
To isolate and identify different fungal species namely Candida, Dermatophytes, Malassezia, and Aspergillus from plaque psoriasis patients, evaluate the association of IL‐17A gene single nucleotide polymorphisms (SNPs) with psoriasis, and to reveal the relation between IL‐17A gene SNPs and the fungal presence within the psoriatic plaques.
Patients/Methods
Fifty plaque psoriasis patients and fifty healthy age and sex volunteers as controls were enrolled in this study. From psoriatic plaques, mycological isolation was done by direct microscopic examination (10% KOH mount), culture onto the three sets of media then species identification by phenotypic procedures. Genomic DNA extraction and genotyping for IL‐17A (rs10484879) SNPs using polymerase chain reaction and restriction fragment length polymorphism were also done.
Results
Psoriasis cases showed higher frequency of fungal growth 86% vs. 14% in controls; (p < 0.001). The frequency of IL‐17A GA, AA, and total polymorphism (GA+AA) genotypes in psoriasis cases was significantly higher than in controls. There was non‐significant association between different IL‐17A genotypes and fungal growth except Aspergillus flavus, which decreased gradually with GG, GA, and AA (37.5%, 20.8%, and 0%, respectively).
Conclusions
Psoriasis cases are significantly associated with fungal growth, which may be a contributing factor in its pathogenesis. SNPs of IL‐17A (rs10484879) G/A gene led to increased susceptibility toward pathogenesis of psoriasis. Fungal growth and IL‐17A GA+AA genotypes are suggested to be independent predictors of psoriasis susceptibility.</abstract><doi>10.1111/jocd.14551</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1473-2130 |
| ispartof | Journal of cosmetic dermatology, 2022-07, Vol.21 (7), p.3059-3067 |
| issn | 1473-2130 1473-2165 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2594290666 |
| source | Wiley Journals |
| subjects | culture fungal infections IL‐17A psoriasis single nucleotide polymorphism |
| title | Interleukin‐17A gene single nucleotide polymorphism and its relation to fungal growth in psoriatic patients: A preliminary study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T09%3A22%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interleukin%E2%80%9017A%20gene%20single%20nucleotide%20polymorphism%20and%20its%20relation%20to%20fungal%20growth%20in%20psoriatic%20patients:%20A%20preliminary%20study&rft.jtitle=Journal%20of%20cosmetic%20dermatology&rft.au=Sanad,%20Eman%20M.%20K.&rft.date=2022-07&rft.volume=21&rft.issue=7&rft.spage=3059&rft.epage=3067&rft.pages=3059-3067&rft.issn=1473-2130&rft.eissn=1473-2165&rft_id=info:doi/10.1111/jocd.14551&rft_dat=%3Cproquest_cross%3E2594290666%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2594290666&rft_id=info:pmid/&rfr_iscdi=true |