Mutation of the rice AN1‐type zinc‐finger protein gene ASL4 causes chloroplast development defects and seedling lethality
Plant zinc‐finger proteins play a crucial role in biosynthesis and plant development. However, it is not known whether certain zinc‐finger proteins play a role in rice chloroplast development. In this study, a novel rice zinc‐finger protein mutant asl4 (albino seedling lethality4), which exhibits an...
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| Veröffentlicht in: | Plant biology (Stuttgart, Germany) Germany), 2022-01, Vol.24 (1), p.95-103 |
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| creator | Lin, D. Z. Pan, Q. W. Wang, X. M. Chen, Y. Pan, X. B. Dong, Y. J. Whelan, J. |
| description | Plant zinc‐finger proteins play a crucial role in biosynthesis and plant development. However, it is not known whether certain zinc‐finger proteins play a role in rice chloroplast development.
In this study, a novel rice zinc‐finger protein mutant asl4 (albino seedling lethality4), which exhibits an albino lethal phenotype at the seedling stage, was used. Chlorophyll fluorescence analysis and TEM were used to investigate features of the asl4 mutant. The genetic behaviour and function of ASL4 gene were then analysed thorough map‐based cloning, transgenic complement and subcellular localization.
The albino lethal phenotype was caused by a single nucleotide (G*) deletion mutation on the exon of the ASL4 (LOC_Os09g21710) gene. The ASL4 gene encoded a novel zinc‐finger protein containing two ZnF‐AN1 domains, which was localized to the nucleocytoplasm. The ASL4 transcripts were highly expressed in all leaves but relatively less in other tissues, suggesting its tissue‐specific expression. The transcript levels of associated genes for Chl biosynthesis, photosynthesis and chloroplast development were severely suppressed in asl4 mutants.
In conclusion, the absence of ASL4 function caused a defect in chloroplast development and seedling lethality. This is the first published report on the importance of the ZnF‐AN1 type zinc‐finger protein gene in chloroplast development in rice.
Disfunction of rice ASL4 gene encoded ZnF‐AN1 type zinc‐finger protein led to chloroplast developmental defect and seedling lethality. |
| doi_str_mv | 10.1111/plb.13334 |
| format | Article |
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In this study, a novel rice zinc‐finger protein mutant asl4 (albino seedling lethality4), which exhibits an albino lethal phenotype at the seedling stage, was used. Chlorophyll fluorescence analysis and TEM were used to investigate features of the asl4 mutant. The genetic behaviour and function of ASL4 gene were then analysed thorough map‐based cloning, transgenic complement and subcellular localization.
The albino lethal phenotype was caused by a single nucleotide (G*) deletion mutation on the exon of the ASL4 (LOC_Os09g21710) gene. The ASL4 gene encoded a novel zinc‐finger protein containing two ZnF‐AN1 domains, which was localized to the nucleocytoplasm. The ASL4 transcripts were highly expressed in all leaves but relatively less in other tissues, suggesting its tissue‐specific expression. The transcript levels of associated genes for Chl biosynthesis, photosynthesis and chloroplast development were severely suppressed in asl4 mutants.
In conclusion, the absence of ASL4 function caused a defect in chloroplast development and seedling lethality. This is the first published report on the importance of the ZnF‐AN1 type zinc‐finger protein gene in chloroplast development in rice.
Disfunction of rice ASL4 gene encoded ZnF‐AN1 type zinc‐finger protein led to chloroplast developmental defect and seedling lethality.</description><identifier>ISSN: 1435-8603</identifier><identifier>EISSN: 1438-8677</identifier><identifier>DOI: 10.1111/plb.13334</identifier><identifier>PMID: 34724300</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Albinism ; albino ; Biosynthesis ; Chlorophyll ; chloroplast development ; Chloroplasts ; Chloroplasts - metabolism ; Cloning ; Fluorescence ; Gene deletion ; Gene Expression Regulation, Plant ; gene mutation ; Lethality ; Localization ; Mutants ; Mutation ; Nucleotides ; Oryza - genetics ; Oryza - metabolism ; Phenotype ; Phenotypes ; Photosynthesis ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Proteins ; Rice ; Seedlings ; Seedlings - genetics ; Seedlings - metabolism ; Transcription ; Zinc ; Zinc finger proteins ; zinc‐finger protein</subject><ispartof>Plant biology (Stuttgart, Germany), 2022-01, Vol.24 (1), p.95-103</ispartof><rights>2021 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands</rights><rights>2021 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.</rights><rights>2022 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-85d87a00813837af3bb63e4b7299078b56a880ffa68b6bb923797276741385a63</citedby><cites>FETCH-LOGICAL-c3534-85d87a00813837af3bb63e4b7299078b56a880ffa68b6bb923797276741385a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fplb.13334$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fplb.13334$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34724300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Whelan, J.</contributor><creatorcontrib>Lin, D. Z.</creatorcontrib><creatorcontrib>Pan, Q. W.</creatorcontrib><creatorcontrib>Wang, X. M.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><creatorcontrib>Pan, X. B.</creatorcontrib><creatorcontrib>Dong, Y. J.</creatorcontrib><creatorcontrib>Whelan, J.</creatorcontrib><title>Mutation of the rice AN1‐type zinc‐finger protein gene ASL4 causes chloroplast development defects and seedling lethality</title><title>Plant biology (Stuttgart, Germany)</title><addtitle>Plant Biol (Stuttg)</addtitle><description>Plant zinc‐finger proteins play a crucial role in biosynthesis and plant development. However, it is not known whether certain zinc‐finger proteins play a role in rice chloroplast development.
In this study, a novel rice zinc‐finger protein mutant asl4 (albino seedling lethality4), which exhibits an albino lethal phenotype at the seedling stage, was used. Chlorophyll fluorescence analysis and TEM were used to investigate features of the asl4 mutant. The genetic behaviour and function of ASL4 gene were then analysed thorough map‐based cloning, transgenic complement and subcellular localization.
The albino lethal phenotype was caused by a single nucleotide (G*) deletion mutation on the exon of the ASL4 (LOC_Os09g21710) gene. The ASL4 gene encoded a novel zinc‐finger protein containing two ZnF‐AN1 domains, which was localized to the nucleocytoplasm. The ASL4 transcripts were highly expressed in all leaves but relatively less in other tissues, suggesting its tissue‐specific expression. The transcript levels of associated genes for Chl biosynthesis, photosynthesis and chloroplast development were severely suppressed in asl4 mutants.
In conclusion, the absence of ASL4 function caused a defect in chloroplast development and seedling lethality. This is the first published report on the importance of the ZnF‐AN1 type zinc‐finger protein gene in chloroplast development in rice.
Disfunction of rice ASL4 gene encoded ZnF‐AN1 type zinc‐finger protein led to chloroplast developmental defect and seedling lethality.</description><subject>Albinism</subject><subject>albino</subject><subject>Biosynthesis</subject><subject>Chlorophyll</subject><subject>chloroplast development</subject><subject>Chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>Cloning</subject><subject>Fluorescence</subject><subject>Gene deletion</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene mutation</subject><subject>Lethality</subject><subject>Localization</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Photosynthesis</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Proteins</subject><subject>Rice</subject><subject>Seedlings</subject><subject>Seedlings - genetics</subject><subject>Seedlings - metabolism</subject><subject>Transcription</subject><subject>Zinc</subject><subject>Zinc finger proteins</subject><subject>zinc‐finger protein</subject><issn>1435-8603</issn><issn>1438-8677</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFqFTEYhYMotl5d-AIS6MYubptMMklmWYutwrUV1HVIZv7pTclNxiSjXEHoI_iMPolpb3VRMJucxfd_HDgIvaTkiNZ3PHl7RBlj_BHap5yppRJSPr7Lbc2E7aFnOV8TQnlH6FO0x7hsOCNkH_38MBdTXAw4jrisASfXAz65oL9vfpXtBPiHC33NowtXkPCUYgEX8BWESn1acdybOUPG_drHFCdvcsEDfAMfpw2E2zxCXzI2YcAZYPDVgz2UtfGubJ-jJ6PxGV7c_wv05ezt59N3y9Xl-fvTk9WyZy3jS9UOShpCFGWKSTMyawUDbmXTdUQq2wqjFBlHI5QV1nYNk51spJC8HrRGsAV6vfPW_l9nyEVvXO7BexMgzlk3bb2hQjWsogcP0Os4p1Db6UaQ6mxZLbFAhzuqTzHnBKOektuYtNWU6NtNdN1E321S2Vf3xtluYPhH_h2hAsc74LvzsP2_SX9cvdkp_wAtV5ah</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Lin, D. Z.</creator><creator>Pan, Q. W.</creator><creator>Wang, X. M.</creator><creator>Chen, Y.</creator><creator>Pan, X. B.</creator><creator>Dong, Y. J.</creator><creator>Whelan, J.</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202201</creationdate><title>Mutation of the rice AN1‐type zinc‐finger protein gene ASL4 causes chloroplast development defects and seedling lethality</title><author>Lin, D. Z. ; Pan, Q. W. ; Wang, X. M. ; Chen, Y. ; Pan, X. B. ; Dong, Y. J. ; Whelan, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-85d87a00813837af3bb63e4b7299078b56a880ffa68b6bb923797276741385a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Albinism</topic><topic>albino</topic><topic>Biosynthesis</topic><topic>Chlorophyll</topic><topic>chloroplast development</topic><topic>Chloroplasts</topic><topic>Chloroplasts - metabolism</topic><topic>Cloning</topic><topic>Fluorescence</topic><topic>Gene deletion</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene mutation</topic><topic>Lethality</topic><topic>Localization</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Photosynthesis</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Proteins</topic><topic>Rice</topic><topic>Seedlings</topic><topic>Seedlings - genetics</topic><topic>Seedlings - metabolism</topic><topic>Transcription</topic><topic>Zinc</topic><topic>Zinc finger proteins</topic><topic>zinc‐finger protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, D. Z.</creatorcontrib><creatorcontrib>Pan, Q. W.</creatorcontrib><creatorcontrib>Wang, X. M.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><creatorcontrib>Pan, X. B.</creatorcontrib><creatorcontrib>Dong, Y. J.</creatorcontrib><creatorcontrib>Whelan, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant biology (Stuttgart, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, D. Z.</au><au>Pan, Q. W.</au><au>Wang, X. M.</au><au>Chen, Y.</au><au>Pan, X. B.</au><au>Dong, Y. J.</au><au>Whelan, J.</au><au>Whelan, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutation of the rice AN1‐type zinc‐finger protein gene ASL4 causes chloroplast development defects and seedling lethality</atitle><jtitle>Plant biology (Stuttgart, Germany)</jtitle><addtitle>Plant Biol (Stuttg)</addtitle><date>2022-01</date><risdate>2022</risdate><volume>24</volume><issue>1</issue><spage>95</spage><epage>103</epage><pages>95-103</pages><issn>1435-8603</issn><eissn>1438-8677</eissn><abstract>Plant zinc‐finger proteins play a crucial role in biosynthesis and plant development. However, it is not known whether certain zinc‐finger proteins play a role in rice chloroplast development.
In this study, a novel rice zinc‐finger protein mutant asl4 (albino seedling lethality4), which exhibits an albino lethal phenotype at the seedling stage, was used. Chlorophyll fluorescence analysis and TEM were used to investigate features of the asl4 mutant. The genetic behaviour and function of ASL4 gene were then analysed thorough map‐based cloning, transgenic complement and subcellular localization.
The albino lethal phenotype was caused by a single nucleotide (G*) deletion mutation on the exon of the ASL4 (LOC_Os09g21710) gene. The ASL4 gene encoded a novel zinc‐finger protein containing two ZnF‐AN1 domains, which was localized to the nucleocytoplasm. The ASL4 transcripts were highly expressed in all leaves but relatively less in other tissues, suggesting its tissue‐specific expression. The transcript levels of associated genes for Chl biosynthesis, photosynthesis and chloroplast development were severely suppressed in asl4 mutants.
In conclusion, the absence of ASL4 function caused a defect in chloroplast development and seedling lethality. This is the first published report on the importance of the ZnF‐AN1 type zinc‐finger protein gene in chloroplast development in rice.
Disfunction of rice ASL4 gene encoded ZnF‐AN1 type zinc‐finger protein led to chloroplast developmental defect and seedling lethality.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34724300</pmid><doi>10.1111/plb.13334</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant biology (Stuttgart, Germany), 2022-01, Vol.24 (1), p.95-103 |
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| subjects | Albinism albino Biosynthesis Chlorophyll chloroplast development Chloroplasts Chloroplasts - metabolism Cloning Fluorescence Gene deletion Gene Expression Regulation, Plant gene mutation Lethality Localization Mutants Mutation Nucleotides Oryza - genetics Oryza - metabolism Phenotype Phenotypes Photosynthesis Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Proteins Rice Seedlings Seedlings - genetics Seedlings - metabolism Transcription Zinc Zinc finger proteins zinc‐finger protein |
| title | Mutation of the rice AN1‐type zinc‐finger protein gene ASL4 causes chloroplast development defects and seedling lethality |
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