High-Throughput CRISPR–Cas13 SARS-CoV-2 Test

Abstract Background The ability to control the spread of COVID-19 continues to be hampered by a lack of rapid, scalable, and easily deployable diagnostic solutions. Methods We developed a diagnostic method based on CRISPR (clustered regularly interspaced short palindromic repeats) that can deliver s...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2022-01, Vol.68 (1), p.172-180
Hauptverfasser:	Manning, Brendan J, Khan, Wahab A, Peña, Jennifer M, Fiore, Elizabeth S, Boisvert, Heike, Tudino, Marisa C, Barney, Rachael E, Wilson, Mary K, Singh, Subha, Mowatt, Joel A, Thompson, Hannah J, Tsongalis, Gregory J, Blake, William J
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 Testing - methods CRISPR CRISPR-Cas Systems Diagnostic systems Disease control DNA sequencing High-Throughput Screening Assays Humans Laboratories Methods Nucleic acids Nucleotide sequencing Reverse transcription RNA, Viral - isolation & purification Saliva SARS-CoV-2 - isolation & purification Sensitivity and Specificity Severe acute respiratory syndrome coronavirus 2 Viral diseases
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