Impacts of high‐intensity exercise on the metabolomics profile of human skeletal muscle tissue
The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high‐intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aero...
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| Veröffentlicht in: | Scandinavian journal of medicine & science in sports 2022-02, Vol.32 (2), p.402-413 |
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| creator | Zagatto, Alessandro M. Bishop, David J. Antunes, Barbara Moura Beck, Wladimir R. Malta, Elvis S. Poli, Rodrigo A.B. Cavaglieri, Claudia R. Chacon‐Mikahil, Mara Patrícia T. Castro, Alex |
| description | The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high‐intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by 1H‐NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 ± 35.5 s whereas the muscle pH at exhaustion was 6.48 ± 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2‐oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p |
| doi_str_mv | 10.1111/sms.14086 |
| format | Article |
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Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by 1H‐NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 ± 35.5 s whereas the muscle pH at exhaustion was 6.48 ± 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2‐oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O‐acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2‐oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (−70.2%), ADP (−56.5%), carnitine (−33.5%), and glutamate (−42.3%). Only the succinate was significantly correlated with tlim (r = −0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high‐intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim.</description><identifier>ISSN: 0905-7188</identifier><identifier>EISSN: 1600-0838</identifier><identifier>DOI: 10.1111/sms.14086</identifier><identifier>PMID: 34706104</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Bicycling ; Exercise ; exercise metabolism ; Exercise Test ; Humans ; Male ; Metabolism ; Metabolites ; metabolomic ; Metabolomics ; Muscle, Skeletal ; Oxidation ; skeletal muscle ; supramaximal effort</subject><ispartof>Scandinavian journal of medicine & science in sports, 2022-02, Vol.32 (2), p.402-413</ispartof><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2022 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4196-d412f9936be96d092e2a392abe2397e245d261283b7f16532ae7ced8105e1d783</citedby><cites>FETCH-LOGICAL-c4196-d412f9936be96d092e2a392abe2397e245d261283b7f16532ae7ced8105e1d783</cites><orcidid>0000-0003-1065-4158 ; 0000-0002-5807-6900 ; 0000-0001-7176-2713</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fsms.14086$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fsms.14086$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34706104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zagatto, Alessandro M.</creatorcontrib><creatorcontrib>Bishop, David J.</creatorcontrib><creatorcontrib>Antunes, Barbara Moura</creatorcontrib><creatorcontrib>Beck, Wladimir R.</creatorcontrib><creatorcontrib>Malta, Elvis S.</creatorcontrib><creatorcontrib>Poli, Rodrigo A.B.</creatorcontrib><creatorcontrib>Cavaglieri, Claudia R.</creatorcontrib><creatorcontrib>Chacon‐Mikahil, Mara Patrícia T.</creatorcontrib><creatorcontrib>Castro, Alex</creatorcontrib><title>Impacts of high‐intensity exercise on the metabolomics profile of human skeletal muscle tissue</title><title>Scandinavian journal of medicine & science in sports</title><addtitle>Scand J Med Sci Sports</addtitle><description>The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high‐intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by 1H‐NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 ± 35.5 s whereas the muscle pH at exhaustion was 6.48 ± 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2‐oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O‐acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2‐oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (−70.2%), ADP (−56.5%), carnitine (−33.5%), and glutamate (−42.3%). Only the succinate was significantly correlated with tlim (r = −0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high‐intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim.</description><subject>Bicycling</subject><subject>Exercise</subject><subject>exercise metabolism</subject><subject>Exercise Test</subject><subject>Humans</subject><subject>Male</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>metabolomic</subject><subject>Metabolomics</subject><subject>Muscle, Skeletal</subject><subject>Oxidation</subject><subject>skeletal muscle</subject><subject>supramaximal effort</subject><issn>0905-7188</issn><issn>1600-0838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kL9O5DAQh60T6FiWK3gBZInmKMLaThzbJVrxTwJRcNQ-J5mwhjhZMom47XgEnpEnwbBAgXTTTDHf_Gb0EbLL2SGPNcOAhzxjOv9BJjxnLGE61RtkwgyTieJab5FtxDvGuDKZ_Em20kyxnLNsQv6eh6UrB6RdTRf-dvHy9OzbAVr0w4rCP-hLj0C7lg4LoAEGV3RNF3yJdNl3tW_gfXEMrqV4D00EGhpGLONg8Igj7JDN2jUIvz76lNycHP-ZnyUXV6fn86OLpMy4yZMq46I2Js0LMHnFjADhUiNcASI1CkQmK5FzodNC1TyXqXCgSqg0ZxJ4pXQ6Jb_XufGvhxFwsMFjCU3jWuhGtEJqpaTkSkZ0_xt61419G7-z8YbWLJNCRepgTZV9h9hDbZe9D65fWc7sm3Ybtdt37ZHd-0gciwDVF_npOQKzNfAYla3-n2SvL6_Xka-YQYzh</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Zagatto, Alessandro M.</creator><creator>Bishop, David J.</creator><creator>Antunes, Barbara Moura</creator><creator>Beck, Wladimir R.</creator><creator>Malta, Elvis S.</creator><creator>Poli, Rodrigo A.B.</creator><creator>Cavaglieri, Claudia R.</creator><creator>Chacon‐Mikahil, Mara Patrícia T.</creator><creator>Castro, Alex</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TS</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1065-4158</orcidid><orcidid>https://orcid.org/0000-0002-5807-6900</orcidid><orcidid>https://orcid.org/0000-0001-7176-2713</orcidid></search><sort><creationdate>202202</creationdate><title>Impacts of high‐intensity exercise on the metabolomics profile of human skeletal muscle tissue</title><author>Zagatto, Alessandro M. ; Bishop, David J. ; Antunes, Barbara Moura ; Beck, Wladimir R. ; Malta, Elvis S. ; Poli, Rodrigo A.B. ; Cavaglieri, Claudia R. ; Chacon‐Mikahil, Mara Patrícia T. ; Castro, Alex</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4196-d412f9936be96d092e2a392abe2397e245d261283b7f16532ae7ced8105e1d783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bicycling</topic><topic>Exercise</topic><topic>exercise metabolism</topic><topic>Exercise Test</topic><topic>Humans</topic><topic>Male</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>metabolomic</topic><topic>Metabolomics</topic><topic>Muscle, Skeletal</topic><topic>Oxidation</topic><topic>skeletal muscle</topic><topic>supramaximal effort</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zagatto, Alessandro M.</creatorcontrib><creatorcontrib>Bishop, David J.</creatorcontrib><creatorcontrib>Antunes, Barbara Moura</creatorcontrib><creatorcontrib>Beck, Wladimir R.</creatorcontrib><creatorcontrib>Malta, Elvis S.</creatorcontrib><creatorcontrib>Poli, Rodrigo A.B.</creatorcontrib><creatorcontrib>Cavaglieri, Claudia R.</creatorcontrib><creatorcontrib>Chacon‐Mikahil, Mara Patrícia T.</creatorcontrib><creatorcontrib>Castro, Alex</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of medicine & science in sports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zagatto, Alessandro M.</au><au>Bishop, David J.</au><au>Antunes, Barbara Moura</au><au>Beck, Wladimir R.</au><au>Malta, Elvis S.</au><au>Poli, Rodrigo A.B.</au><au>Cavaglieri, Claudia R.</au><au>Chacon‐Mikahil, Mara Patrícia T.</au><au>Castro, Alex</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impacts of high‐intensity exercise on the metabolomics profile of human skeletal muscle tissue</atitle><jtitle>Scandinavian journal of medicine & science in sports</jtitle><addtitle>Scand J Med Sci Sports</addtitle><date>2022-02</date><risdate>2022</risdate><volume>32</volume><issue>2</issue><spage>402</spage><epage>413</epage><pages>402-413</pages><issn>0905-7188</issn><eissn>1600-0838</eissn><abstract>The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high‐intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by 1H‐NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 ± 35.5 s whereas the muscle pH at exhaustion was 6.48 ± 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2‐oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O‐acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2‐oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (−70.2%), ADP (−56.5%), carnitine (−33.5%), and glutamate (−42.3%). Only the succinate was significantly correlated with tlim (r = −0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high‐intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>34706104</pmid><doi>10.1111/sms.14086</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1065-4158</orcidid><orcidid>https://orcid.org/0000-0002-5807-6900</orcidid><orcidid>https://orcid.org/0000-0001-7176-2713</orcidid></addata></record> |
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| subjects | Bicycling Exercise exercise metabolism Exercise Test Humans Male Metabolism Metabolites metabolomic Metabolomics Muscle, Skeletal Oxidation skeletal muscle supramaximal effort |
| title | Impacts of high‐intensity exercise on the metabolomics profile of human skeletal muscle tissue |
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