Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran
Purpose Toxoplasma gondii is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping T. gondii strains from slaughtered goats in Jahrom. Methods A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughter...
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| Veröffentlicht in: | Acta parasitologica 2022-03, Vol.67 (1), p.454-459 |
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| creator | Sadooni, Riam Rezanezhad, Hassan Solhjoo, Kavos Kalantari, Mohsen Pourmohammadi, Behrad Erfanian, Saiedeh Armand, Belal Esmi Jahromi, Masoud |
| description | Purpose
Toxoplasma gondii
is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping
T. gondii
strains from slaughtered goats in Jahrom.
Methods
A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughtered goats were collected from Jahrom slaughterhouse. After DNA extraction, the
T. gondii
strains were genotyped by the nested PCR–RFLP based on GRA6 and 3ʹ, and 5ʹ ends of the SAG2 gene.
Results
T. gondii
infection was present in 18.2% of cases. Among the examined organs, the diaphragm was more disposed to the infection (10.2%). Furthermore, infection rates of the heart and tongue were 8.6% and 3.7%, respectively. Concurrent infection in the heart and diaphragm, tongue and diaphragm, and heart and tongue were 3.2%, 0.5%, and 0.5%, respectively. In genotyping experiments, genotype I was the most frequent genotype of
T. gondii
(58.8%), followed by type II (23.5%), type III (11.8%), and a combination of type I and II (5.9%).
Conclusions
The results of this study showed the presence of different genotypes of
T. gondii
in goats including three major and mixed genotypes. These results can be useful in toxoplasmosis control and prevention. |
| doi_str_mv | 10.1007/s11686-021-00481-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2587733458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2641555457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c326t-3ca630e851784ce09186cc37ca3e4b4b5461adf9c2e992ff5a4031875b23b4553</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMotl5ewIUE3LhwNPfJLKVqVQourOuQSTNtSiepyQzo25taL-DCVU443_nP4QPgBKNLjFB5lTAWUhSI4AIhJnEhdsAQy0oUWHK8m2tCUUEkwQNwkNIyQ0JKuQ8GlJWo4gwNwXRsfeje187PYWjgNLyF9UqnVsN58DPn4HMXtfMJNjG0cBx0l6Dz8FEvNv8bl7roTHcBn0PfLWz08CFqfwT2Gr1K9vjrPQQvd7fT0X0xeRo_jK4nhaFEdAU1WlBk862lZMaiCkthDC2NppbVrOZMYD1rKkNsVZGm4ZohimXJa0Jrxjk9BOfb3HUMr71NnWpdMna10t6GPinCZVlSyrjM6NkfdBn66PN1igiGOeeMl5kiW8rEkFK0jVpH1-r4rjBSG-dq61xl5-rTuRJ56PQruq9bO_sZ-ZacAboFUm75uY2_u_-J_QDkX4r-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2641555457</pqid></control><display><type>article</type><title>Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Sadooni, Riam ; Rezanezhad, Hassan ; Solhjoo, Kavos ; Kalantari, Mohsen ; Pourmohammadi, Behrad ; Erfanian, Saiedeh ; Armand, Belal ; Esmi Jahromi, Masoud</creator><creatorcontrib>Sadooni, Riam ; Rezanezhad, Hassan ; Solhjoo, Kavos ; Kalantari, Mohsen ; Pourmohammadi, Behrad ; Erfanian, Saiedeh ; Armand, Belal ; Esmi Jahromi, Masoud</creatorcontrib><description>Purpose
Toxoplasma gondii
is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping
T. gondii
strains from slaughtered goats in Jahrom.
Methods
A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughtered goats were collected from Jahrom slaughterhouse. After DNA extraction, the
T. gondii
strains were genotyped by the nested PCR–RFLP based on GRA6 and 3ʹ, and 5ʹ ends of the SAG2 gene.
Results
T. gondii
infection was present in 18.2% of cases. Among the examined organs, the diaphragm was more disposed to the infection (10.2%). Furthermore, infection rates of the heart and tongue were 8.6% and 3.7%, respectively. Concurrent infection in the heart and diaphragm, tongue and diaphragm, and heart and tongue were 3.2%, 0.5%, and 0.5%, respectively. In genotyping experiments, genotype I was the most frequent genotype of
T. gondii
(58.8%), followed by type II (23.5%), type III (11.8%), and a combination of type I and II (5.9%).
Conclusions
The results of this study showed the presence of different genotypes of
T. gondii
in goats including three major and mixed genotypes. These results can be useful in toxoplasmosis control and prevention.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1007/s11686-021-00481-6</identifier><identifier>PMID: 34709540</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Abattoirs ; Animal Systematics/Taxonomy/Biogeography ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Concurrent infection ; Cysts ; Diaphragm ; DNA, Protozoan - genetics ; Ecology ; Food consumption ; Food contamination ; Genotype ; Genotypes ; Genotyping ; Goats ; Heart ; Infections ; Iran - epidemiology ; Medical Microbiology ; Microbiology ; Oocysts ; Organs ; Original Paper ; Parasitology ; Polymorphism, Restriction Fragment Length ; Protozoa ; Strains (organisms) ; Tongue ; Toxoplasma - genetics ; Toxoplasma gondii ; Toxoplasmosis ; Toxoplasmosis, Animal - epidemiology ; Water pollution</subject><ispartof>Acta parasitologica, 2022-03, Vol.67 (1), p.454-459</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021</rights><rights>2021. Witold Stefański Institute of Parasitology, Polish Academy of Sciences.</rights><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-3ca630e851784ce09186cc37ca3e4b4b5461adf9c2e992ff5a4031875b23b4553</cites><orcidid>0000-0002-7448-3215</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11686-021-00481-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11686-021-00481-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34709540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadooni, Riam</creatorcontrib><creatorcontrib>Rezanezhad, Hassan</creatorcontrib><creatorcontrib>Solhjoo, Kavos</creatorcontrib><creatorcontrib>Kalantari, Mohsen</creatorcontrib><creatorcontrib>Pourmohammadi, Behrad</creatorcontrib><creatorcontrib>Erfanian, Saiedeh</creatorcontrib><creatorcontrib>Armand, Belal</creatorcontrib><creatorcontrib>Esmi Jahromi, Masoud</creatorcontrib><title>Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Purpose
Toxoplasma gondii
is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping
T. gondii
strains from slaughtered goats in Jahrom.
Methods
A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughtered goats were collected from Jahrom slaughterhouse. After DNA extraction, the
T. gondii
strains were genotyped by the nested PCR–RFLP based on GRA6 and 3ʹ, and 5ʹ ends of the SAG2 gene.
Results
T. gondii
infection was present in 18.2% of cases. Among the examined organs, the diaphragm was more disposed to the infection (10.2%). Furthermore, infection rates of the heart and tongue were 8.6% and 3.7%, respectively. Concurrent infection in the heart and diaphragm, tongue and diaphragm, and heart and tongue were 3.2%, 0.5%, and 0.5%, respectively. In genotyping experiments, genotype I was the most frequent genotype of
T. gondii
(58.8%), followed by type II (23.5%), type III (11.8%), and a combination of type I and II (5.9%).
Conclusions
The results of this study showed the presence of different genotypes of
T. gondii
in goats including three major and mixed genotypes. These results can be useful in toxoplasmosis control and prevention.</description><subject>Abattoirs</subject><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Concurrent infection</subject><subject>Cysts</subject><subject>Diaphragm</subject><subject>DNA, Protozoan - genetics</subject><subject>Ecology</subject><subject>Food consumption</subject><subject>Food contamination</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Goats</subject><subject>Heart</subject><subject>Infections</subject><subject>Iran - epidemiology</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Oocysts</subject><subject>Organs</subject><subject>Original Paper</subject><subject>Parasitology</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Protozoa</subject><subject>Strains (organisms)</subject><subject>Tongue</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Water pollution</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotl5ewIUE3LhwNPfJLKVqVQourOuQSTNtSiepyQzo25taL-DCVU443_nP4QPgBKNLjFB5lTAWUhSI4AIhJnEhdsAQy0oUWHK8m2tCUUEkwQNwkNIyQ0JKuQ8GlJWo4gwNwXRsfeje187PYWjgNLyF9UqnVsN58DPn4HMXtfMJNjG0cBx0l6Dz8FEvNv8bl7roTHcBn0PfLWz08CFqfwT2Gr1K9vjrPQQvd7fT0X0xeRo_jK4nhaFEdAU1WlBk862lZMaiCkthDC2NppbVrOZMYD1rKkNsVZGm4ZohimXJa0Jrxjk9BOfb3HUMr71NnWpdMna10t6GPinCZVlSyrjM6NkfdBn66PN1igiGOeeMl5kiW8rEkFK0jVpH1-r4rjBSG-dq61xl5-rTuRJ56PQruq9bO_sZ-ZacAboFUm75uY2_u_-J_QDkX4r-</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Sadooni, Riam</creator><creator>Rezanezhad, Hassan</creator><creator>Solhjoo, Kavos</creator><creator>Kalantari, Mohsen</creator><creator>Pourmohammadi, Behrad</creator><creator>Erfanian, Saiedeh</creator><creator>Armand, Belal</creator><creator>Esmi Jahromi, Masoud</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7448-3215</orcidid></search><sort><creationdate>20220301</creationdate><title>Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran</title><author>Sadooni, Riam ; Rezanezhad, Hassan ; Solhjoo, Kavos ; Kalantari, Mohsen ; Pourmohammadi, Behrad ; Erfanian, Saiedeh ; Armand, Belal ; Esmi Jahromi, Masoud</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-3ca630e851784ce09186cc37ca3e4b4b5461adf9c2e992ff5a4031875b23b4553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abattoirs</topic><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Concurrent infection</topic><topic>Cysts</topic><topic>Diaphragm</topic><topic>DNA, Protozoan - genetics</topic><topic>Ecology</topic><topic>Food consumption</topic><topic>Food contamination</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Genotyping</topic><topic>Goats</topic><topic>Heart</topic><topic>Infections</topic><topic>Iran - epidemiology</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Oocysts</topic><topic>Organs</topic><topic>Original Paper</topic><topic>Parasitology</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Protozoa</topic><topic>Strains (organisms)</topic><topic>Tongue</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Water pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadooni, Riam</creatorcontrib><creatorcontrib>Rezanezhad, Hassan</creatorcontrib><creatorcontrib>Solhjoo, Kavos</creatorcontrib><creatorcontrib>Kalantari, Mohsen</creatorcontrib><creatorcontrib>Pourmohammadi, Behrad</creatorcontrib><creatorcontrib>Erfanian, Saiedeh</creatorcontrib><creatorcontrib>Armand, Belal</creatorcontrib><creatorcontrib>Esmi Jahromi, Masoud</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadooni, Riam</au><au>Rezanezhad, Hassan</au><au>Solhjoo, Kavos</au><au>Kalantari, Mohsen</au><au>Pourmohammadi, Behrad</au><au>Erfanian, Saiedeh</au><au>Armand, Belal</au><au>Esmi Jahromi, Masoud</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>67</volume><issue>1</issue><spage>454</spage><epage>459</epage><pages>454-459</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Purpose
Toxoplasma gondii
is transmitted congenitally or acquired by consumption of food and water contaminated with cysts or oocysts. This study aimed at genotyping
T. gondii
strains from slaughtered goats in Jahrom.
Methods
A total of 561 specimens (heart, diaphragm, and tongue) from 187 slaughtered goats were collected from Jahrom slaughterhouse. After DNA extraction, the
T. gondii
strains were genotyped by the nested PCR–RFLP based on GRA6 and 3ʹ, and 5ʹ ends of the SAG2 gene.
Results
T. gondii
infection was present in 18.2% of cases. Among the examined organs, the diaphragm was more disposed to the infection (10.2%). Furthermore, infection rates of the heart and tongue were 8.6% and 3.7%, respectively. Concurrent infection in the heart and diaphragm, tongue and diaphragm, and heart and tongue were 3.2%, 0.5%, and 0.5%, respectively. In genotyping experiments, genotype I was the most frequent genotype of
T. gondii
(58.8%), followed by type II (23.5%), type III (11.8%), and a combination of type I and II (5.9%).
Conclusions
The results of this study showed the presence of different genotypes of
T. gondii
in goats including three major and mixed genotypes. These results can be useful in toxoplasmosis control and prevention.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>34709540</pmid><doi>10.1007/s11686-021-00481-6</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-7448-3215</orcidid></addata></record> |
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| ispartof | Acta parasitologica, 2022-03, Vol.67 (1), p.454-459 |
| issn | 1230-2821 1896-1851 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2587733458 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Abattoirs Animal Systematics/Taxonomy/Biogeography Animals Biomedical and Life Sciences Biomedicine Concurrent infection Cysts Diaphragm DNA, Protozoan - genetics Ecology Food consumption Food contamination Genotype Genotypes Genotyping Goats Heart Infections Iran - epidemiology Medical Microbiology Microbiology Oocysts Organs Original Paper Parasitology Polymorphism, Restriction Fragment Length Protozoa Strains (organisms) Tongue Toxoplasma - genetics Toxoplasma gondii Toxoplasmosis Toxoplasmosis, Animal - epidemiology Water pollution |
| title | Genotyping of Toxoplasma gondii Strains from Goats in Jahrom District, Southern Iran |
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