LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis
Background and Aim Esophageal squamous cell carcinoma (ESCC) is the most significant subtype of esophageal cancer featured with high occurrence. Long noncoding RNAs (lncRNAs) have been proved to modulate the biological properties of cancer cells, including cell proliferation, invasion, migration, an...
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| Veröffentlicht in: | Journal of gastroenterology and hepatology 2022-03, Vol.37 (3), p.507-517 |
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| creator | Wu, Kaiming Wang, Ziao Huang, Yunlong Yao, Long Kang, Ningning Ge, Wei Zhang, Renquan He, Wei |
| description | Background and Aim
Esophageal squamous cell carcinoma (ESCC) is the most significant subtype of esophageal cancer featured with high occurrence. Long noncoding RNAs (lncRNAs) have been proved to modulate the biological properties of cancer cells, including cell proliferation, invasion, migration, and apoptosis. LncRNA protein tyrosine phosphatase receptor type G‐antisense RNA 1 (PTPRG‐AS1) has been reported to play as an oncogene in diverse cancers. However, the detailed function PTPRG‐AS1 may exert in ESCC is unclear.
Methods
PTPRG‐AS1 expression in ESCC cells was investigated via quantitative reverse transcription real‐time polymerase chain reaction (RT‐qPCR). The effects of PTPRG‐AS1 on ESCC cell proliferation, migration, glycolysis, and stemness were verified through functional assays. Mechanism assays including RIP assay, RNA pull down assay, and luciferase reporter assays were performed to verify the molecular mechanism of PTPRG‐AS1.
Results
PTPRG‐AS1 silencing hindered the proliferation, migration, glycolysis and stemness of ESCC cells. PTPRG‐AS1 regulated pyruvate dehydrogenase kinase 1 (PDK1) expression via sponging miR‐599. The PTPRG‐AS1/miR‐599/PDK1 axis was further verified to aggravate the progression of ESCC cells.
Conclusion
PTPRG‐AS1 sponged miR‐599 to up‐regulate PDK1 expression, thereby promoting the proliferation and migration as well as glycolysis and stemness properties of ESCC cells. |
| doi_str_mv | 10.1111/jgh.15719 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2584437914</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2637086189</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3539-177523b6b8eeb5c535cbc352b27cbbfcb7e83802b24f1935b801da0f7f7a5b173</originalsourceid><addsrcrecordid>eNp1kcFuEzEQhi0EoqFw4AWQJS5wSGOv7bX3GLWQAlGJQjlbtuNNHO2uU8-u2ty4cuMZ-yR1m5YDEnMZjebTP__oR-gtJSc012S73pxQIWn1DI0o52RMJS-foxFRVIwrRqsj9ApgSwjhRIqX6IjxUpZCqRH6Pe_c8mKKF5eL5ez215_pD4pr40ITetN7wOtm72KzhwDYdCsMvW87D4B3Ke586kNGYo09xN3GrL1pMFwNpo0DYOebBjuTXOhiax5GwP0mxWG9wW1Y5mOiqiaLs28Um5sAr9GL2jTg3zz2Y_Tz86fL0_Px_Pvsy-l0PnZMsCq_JkXBbGmV91Y4wYSzeVPYQjpra2elV0yRPPOaVkxYRejKkFrW0ghLJTtGHw66-YWrwUOv2wD37kzns29dCMU5kxXlGX3_D7qNQ-qyO12UTBJVUlVl6uOBcikCJF_rXQqtSXtNib7PR-d89EM-mX33qDjY1q_-kk-BZGByAK5D4_f_V9JfZ-cHyTtmVpuw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2637086189</pqid></control><display><type>article</type><title>LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Wu, Kaiming ; Wang, Ziao ; Huang, Yunlong ; Yao, Long ; Kang, Ningning ; Ge, Wei ; Zhang, Renquan ; He, Wei</creator><creatorcontrib>Wu, Kaiming ; Wang, Ziao ; Huang, Yunlong ; Yao, Long ; Kang, Ningning ; Ge, Wei ; Zhang, Renquan ; He, Wei</creatorcontrib><description>Background and Aim
Esophageal squamous cell carcinoma (ESCC) is the most significant subtype of esophageal cancer featured with high occurrence. Long noncoding RNAs (lncRNAs) have been proved to modulate the biological properties of cancer cells, including cell proliferation, invasion, migration, and apoptosis. LncRNA protein tyrosine phosphatase receptor type G‐antisense RNA 1 (PTPRG‐AS1) has been reported to play as an oncogene in diverse cancers. However, the detailed function PTPRG‐AS1 may exert in ESCC is unclear.
Methods
PTPRG‐AS1 expression in ESCC cells was investigated via quantitative reverse transcription real‐time polymerase chain reaction (RT‐qPCR). The effects of PTPRG‐AS1 on ESCC cell proliferation, migration, glycolysis, and stemness were verified through functional assays. Mechanism assays including RIP assay, RNA pull down assay, and luciferase reporter assays were performed to verify the molecular mechanism of PTPRG‐AS1.
Results
PTPRG‐AS1 silencing hindered the proliferation, migration, glycolysis and stemness of ESCC cells. PTPRG‐AS1 regulated pyruvate dehydrogenase kinase 1 (PDK1) expression via sponging miR‐599. The PTPRG‐AS1/miR‐599/PDK1 axis was further verified to aggravate the progression of ESCC cells.
Conclusion
PTPRG‐AS1 sponged miR‐599 to up‐regulate PDK1 expression, thereby promoting the proliferation and migration as well as glycolysis and stemness properties of ESCC cells.</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1111/jgh.15719</identifier><identifier>PMID: 34676588</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>Antisense RNA ; Apoptosis ; Cell growth ; Cell migration ; Cell proliferation ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - metabolism ; Esophageal squamous cell carcinoma ; Esophageal Squamous Cell Carcinoma - genetics ; Esophageal Squamous Cell Carcinoma - metabolism ; Esophagus ; Glycolysis ; Glycolysis - genetics ; Humans ; Kinases ; MicroRNAs - metabolism ; miR‐599 ; Non-coding RNA ; PDK1 ; Phosphoric Monoester Hydrolases - genetics ; Phosphoric Monoester Hydrolases - metabolism ; Polymerase chain reaction ; Protein-tyrosine-phosphatase ; PTPRG‐AS1 ; Pyruvate Dehydrogenase Acetyl-Transferring Kinase - metabolism ; Pyruvic acid ; Reverse transcription ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Signal Transduction ; Squamous cell carcinoma</subject><ispartof>Journal of gastroenterology and hepatology, 2022-03, Vol.37 (3), p.507-517</ispartof><rights>2021 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd</rights><rights>2021 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd.</rights><rights>2022 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-177523b6b8eeb5c535cbc352b27cbbfcb7e83802b24f1935b801da0f7f7a5b173</citedby><cites>FETCH-LOGICAL-c3539-177523b6b8eeb5c535cbc352b27cbbfcb7e83802b24f1935b801da0f7f7a5b173</cites><orcidid>0000-0003-2138-4691</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjgh.15719$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjgh.15719$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34676588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Kaiming</creatorcontrib><creatorcontrib>Wang, Ziao</creatorcontrib><creatorcontrib>Huang, Yunlong</creatorcontrib><creatorcontrib>Yao, Long</creatorcontrib><creatorcontrib>Kang, Ningning</creatorcontrib><creatorcontrib>Ge, Wei</creatorcontrib><creatorcontrib>Zhang, Renquan</creatorcontrib><creatorcontrib>He, Wei</creatorcontrib><title>LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis</title><title>Journal of gastroenterology and hepatology</title><addtitle>J Gastroenterol Hepatol</addtitle><description>Background and Aim
Esophageal squamous cell carcinoma (ESCC) is the most significant subtype of esophageal cancer featured with high occurrence. Long noncoding RNAs (lncRNAs) have been proved to modulate the biological properties of cancer cells, including cell proliferation, invasion, migration, and apoptosis. LncRNA protein tyrosine phosphatase receptor type G‐antisense RNA 1 (PTPRG‐AS1) has been reported to play as an oncogene in diverse cancers. However, the detailed function PTPRG‐AS1 may exert in ESCC is unclear.
Methods
PTPRG‐AS1 expression in ESCC cells was investigated via quantitative reverse transcription real‐time polymerase chain reaction (RT‐qPCR). The effects of PTPRG‐AS1 on ESCC cell proliferation, migration, glycolysis, and stemness were verified through functional assays. Mechanism assays including RIP assay, RNA pull down assay, and luciferase reporter assays were performed to verify the molecular mechanism of PTPRG‐AS1.
Results
PTPRG‐AS1 silencing hindered the proliferation, migration, glycolysis and stemness of ESCC cells. PTPRG‐AS1 regulated pyruvate dehydrogenase kinase 1 (PDK1) expression via sponging miR‐599. The PTPRG‐AS1/miR‐599/PDK1 axis was further verified to aggravate the progression of ESCC cells.
Conclusion
PTPRG‐AS1 sponged miR‐599 to up‐regulate PDK1 expression, thereby promoting the proliferation and migration as well as glycolysis and stemness properties of ESCC cells.</description><subject>Antisense RNA</subject><subject>Apoptosis</subject><subject>Cell growth</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Esophageal squamous cell carcinoma</subject><subject>Esophageal Squamous Cell Carcinoma - genetics</subject><subject>Esophageal Squamous Cell Carcinoma - metabolism</subject><subject>Esophagus</subject><subject>Glycolysis</subject><subject>Glycolysis - genetics</subject><subject>Humans</subject><subject>Kinases</subject><subject>MicroRNAs - metabolism</subject><subject>miR‐599</subject><subject>Non-coding RNA</subject><subject>PDK1</subject><subject>Phosphoric Monoester Hydrolases - genetics</subject><subject>Phosphoric Monoester Hydrolases - metabolism</subject><subject>Polymerase chain reaction</subject><subject>Protein-tyrosine-phosphatase</subject><subject>PTPRG‐AS1</subject><subject>Pyruvate Dehydrogenase Acetyl-Transferring Kinase - metabolism</subject><subject>Pyruvic acid</subject><subject>Reverse transcription</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Signal Transduction</subject><subject>Squamous cell carcinoma</subject><issn>0815-9319</issn><issn>1440-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFuEzEQhi0EoqFw4AWQJS5wSGOv7bX3GLWQAlGJQjlbtuNNHO2uU8-u2ty4cuMZ-yR1m5YDEnMZjebTP__oR-gtJSc012S73pxQIWn1DI0o52RMJS-foxFRVIwrRqsj9ApgSwjhRIqX6IjxUpZCqRH6Pe_c8mKKF5eL5ez215_pD4pr40ITetN7wOtm72KzhwDYdCsMvW87D4B3Ke586kNGYo09xN3GrL1pMFwNpo0DYOebBjuTXOhiax5GwP0mxWG9wW1Y5mOiqiaLs28Um5sAr9GL2jTg3zz2Y_Tz86fL0_Px_Pvsy-l0PnZMsCq_JkXBbGmV91Y4wYSzeVPYQjpra2elV0yRPPOaVkxYRejKkFrW0ghLJTtGHw66-YWrwUOv2wD37kzns29dCMU5kxXlGX3_D7qNQ-qyO12UTBJVUlVl6uOBcikCJF_rXQqtSXtNib7PR-d89EM-mX33qDjY1q_-kk-BZGByAK5D4_f_V9JfZ-cHyTtmVpuw</recordid><startdate>202203</startdate><enddate>202203</enddate><creator>Wu, Kaiming</creator><creator>Wang, Ziao</creator><creator>Huang, Yunlong</creator><creator>Yao, Long</creator><creator>Kang, Ningning</creator><creator>Ge, Wei</creator><creator>Zhang, Renquan</creator><creator>He, Wei</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2138-4691</orcidid></search><sort><creationdate>202203</creationdate><title>LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis</title><author>Wu, Kaiming ; Wang, Ziao ; Huang, Yunlong ; Yao, Long ; Kang, Ningning ; Ge, Wei ; Zhang, Renquan ; He, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-177523b6b8eeb5c535cbc352b27cbbfcb7e83802b24f1935b801da0f7f7a5b173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antisense RNA</topic><topic>Apoptosis</topic><topic>Cell growth</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Esophageal squamous cell carcinoma</topic><topic>Esophageal Squamous Cell Carcinoma - genetics</topic><topic>Esophageal Squamous Cell Carcinoma - metabolism</topic><topic>Esophagus</topic><topic>Glycolysis</topic><topic>Glycolysis - genetics</topic><topic>Humans</topic><topic>Kinases</topic><topic>MicroRNAs - metabolism</topic><topic>miR‐599</topic><topic>Non-coding RNA</topic><topic>PDK1</topic><topic>Phosphoric Monoester Hydrolases - genetics</topic><topic>Phosphoric Monoester Hydrolases - metabolism</topic><topic>Polymerase chain reaction</topic><topic>Protein-tyrosine-phosphatase</topic><topic>PTPRG‐AS1</topic><topic>Pyruvate Dehydrogenase Acetyl-Transferring Kinase - metabolism</topic><topic>Pyruvic acid</topic><topic>Reverse transcription</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Signal Transduction</topic><topic>Squamous cell carcinoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Kaiming</creatorcontrib><creatorcontrib>Wang, Ziao</creatorcontrib><creatorcontrib>Huang, Yunlong</creatorcontrib><creatorcontrib>Yao, Long</creatorcontrib><creatorcontrib>Kang, Ningning</creatorcontrib><creatorcontrib>Ge, Wei</creatorcontrib><creatorcontrib>Zhang, Renquan</creatorcontrib><creatorcontrib>He, Wei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Kaiming</au><au>Wang, Ziao</au><au>Huang, Yunlong</au><au>Yao, Long</au><au>Kang, Ningning</au><au>Ge, Wei</au><au>Zhang, Renquan</au><au>He, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis</atitle><jtitle>Journal of gastroenterology and hepatology</jtitle><addtitle>J Gastroenterol Hepatol</addtitle><date>2022-03</date><risdate>2022</risdate><volume>37</volume><issue>3</issue><spage>507</spage><epage>517</epage><pages>507-517</pages><issn>0815-9319</issn><eissn>1440-1746</eissn><abstract>Background and Aim
Esophageal squamous cell carcinoma (ESCC) is the most significant subtype of esophageal cancer featured with high occurrence. Long noncoding RNAs (lncRNAs) have been proved to modulate the biological properties of cancer cells, including cell proliferation, invasion, migration, and apoptosis. LncRNA protein tyrosine phosphatase receptor type G‐antisense RNA 1 (PTPRG‐AS1) has been reported to play as an oncogene in diverse cancers. However, the detailed function PTPRG‐AS1 may exert in ESCC is unclear.
Methods
PTPRG‐AS1 expression in ESCC cells was investigated via quantitative reverse transcription real‐time polymerase chain reaction (RT‐qPCR). The effects of PTPRG‐AS1 on ESCC cell proliferation, migration, glycolysis, and stemness were verified through functional assays. Mechanism assays including RIP assay, RNA pull down assay, and luciferase reporter assays were performed to verify the molecular mechanism of PTPRG‐AS1.
Results
PTPRG‐AS1 silencing hindered the proliferation, migration, glycolysis and stemness of ESCC cells. PTPRG‐AS1 regulated pyruvate dehydrogenase kinase 1 (PDK1) expression via sponging miR‐599. The PTPRG‐AS1/miR‐599/PDK1 axis was further verified to aggravate the progression of ESCC cells.
Conclusion
PTPRG‐AS1 sponged miR‐599 to up‐regulate PDK1 expression, thereby promoting the proliferation and migration as well as glycolysis and stemness properties of ESCC cells.</abstract><cop>Australia</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34676588</pmid><doi>10.1111/jgh.15719</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2138-4691</orcidid></addata></record> |
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| subjects | Antisense RNA Apoptosis Cell growth Cell migration Cell proliferation Esophageal cancer Esophageal Neoplasms - genetics Esophageal Neoplasms - metabolism Esophageal squamous cell carcinoma Esophageal Squamous Cell Carcinoma - genetics Esophageal Squamous Cell Carcinoma - metabolism Esophagus Glycolysis Glycolysis - genetics Humans Kinases MicroRNAs - metabolism miR‐599 Non-coding RNA PDK1 Phosphoric Monoester Hydrolases - genetics Phosphoric Monoester Hydrolases - metabolism Polymerase chain reaction Protein-tyrosine-phosphatase PTPRG‐AS1 Pyruvate Dehydrogenase Acetyl-Transferring Kinase - metabolism Pyruvic acid Reverse transcription RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Signal Transduction Squamous cell carcinoma |
| title | LncRNA PTPRG‐AS1 facilitates glycolysis and stemness properties of esophageal squamous cell carcinoma cells through miR‐599/PDK1 axis |
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