The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock
Background The comprehension of genome organization and gene modulation is essential for understanding pathogens’ infection mechanisms. Mycoplasma hyopneumoniae 7448 genome is organized in transcriptional units (TUs), which are flanked by regulatory elements such as putative promoters, terminators a...
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| Veröffentlicht in: | Molecular biology reports 2022, Vol.49 (1), p.139-147 |
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| creator | Merker Breyer, Gabriela Malvessi Cattani, Amanda Silveira Schrank, Irene Maboni Siqueira, Franciele |
| description | Background
The comprehension of genome organization and gene modulation is essential for understanding pathogens’ infection mechanisms.
Mycoplasma hyopneumoniae
7448 genome is organized in transcriptional units (TUs), which are flanked by regulatory elements such as putative promoters, terminators and repetitive sequences. Yet the relationship between the presence of these elements and bacterial responses during stress conditions remains unclear. Thus, in this study, in silico and RT-qPCR analyses were associated to determine the effect of regulatory elements in gene expression regulation upon heat shock and oxidative stress conditions.
Methods and results
Thirteen TU’s organizational profiles were found based on promoters and terminators distribution. Differential expression in genes sharing the same TUs was observed, suggesting the activity of internal regulatory elements. Moreover, 88.8% of tested genes were differentially expressed under oxidative stress in comparison to the control condition, being 81.3% of them surrounded by their own regulatory elements. Similarly, under heat shock, 44.4% of the genes showed regulation when compared to control condition, being 75.0% of them surrounded by their own regulatory elements.
Conclusions
Altogether, this data suggests the activity of internal regulatory elements in gene modulation of
M. hyopneumoniae
7448 transcription. |
| doi_str_mv | 10.1007/s11033-021-06851-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2584437777</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2618382607</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-1aa66e646dc79d09aadaadd9d91a95d0403f31b4a3940cd203456878f44aa3</originalsourceid><addsrcrecordid>eNp9kcuKFTEQhoMoznH0BVxIwI2b1krn1r2UwRuMCDL7UNOpntNjd9ImafE8gO9tjmdUcGEoyKK--kPqY-ypgJcCwL7KQoCUDbSiAdNp0dh7bCe0lY3qbXef7UCCaFTtnLFHOd8CgBJWP2RnUhlrNOgd-3G1Jz6Fcd4oDMTjyBPdbDOWmA6cZloolMxj4B8PQ1xnzAvy_SGugbYlhgmJW6U6XhKGPKRpLVMMONeQvMaQifstTeGGx--TxzJ9I55L7WWOwfM9YeF5H4cvj9mDEedMT-7uc_b57Zuri_fN5ad3Hy5eXzaDtLo0AtEYMsr4wfYeekRfy_e-F9hrDwrkKMW1QtkrGHwLUmnT2W5UClGesxen0DXFrxvl4pYpDzTPGChu2bW6U0raeir6_B_0Nm6pfqxSRnSyaw0cqfZEDSnmnGh0a5oWTAcnwB0NuZMhVw25X4bccejZXfR2vZD_M_JbSQXkCcjrcXWU_r79n9if9WieEA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2618382607</pqid></control><display><type>article</type><title>The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Merker Breyer, Gabriela ; Malvessi Cattani, Amanda ; Silveira Schrank, Irene ; Maboni Siqueira, Franciele</creator><creatorcontrib>Merker Breyer, Gabriela ; Malvessi Cattani, Amanda ; Silveira Schrank, Irene ; Maboni Siqueira, Franciele</creatorcontrib><description>Background
The comprehension of genome organization and gene modulation is essential for understanding pathogens’ infection mechanisms.
Mycoplasma hyopneumoniae
7448 genome is organized in transcriptional units (TUs), which are flanked by regulatory elements such as putative promoters, terminators and repetitive sequences. Yet the relationship between the presence of these elements and bacterial responses during stress conditions remains unclear. Thus, in this study, in silico and RT-qPCR analyses were associated to determine the effect of regulatory elements in gene expression regulation upon heat shock and oxidative stress conditions.
Methods and results
Thirteen TU’s organizational profiles were found based on promoters and terminators distribution. Differential expression in genes sharing the same TUs was observed, suggesting the activity of internal regulatory elements. Moreover, 88.8% of tested genes were differentially expressed under oxidative stress in comparison to the control condition, being 81.3% of them surrounded by their own regulatory elements. Similarly, under heat shock, 44.4% of the genes showed regulation when compared to control condition, being 75.0% of them surrounded by their own regulatory elements.
Conclusions
Altogether, this data suggests the activity of internal regulatory elements in gene modulation of
M. hyopneumoniae
7448 transcription.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-021-06851-7</identifier><identifier>PMID: 34676505</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Bacterial ; Gene regulation ; Genomes ; Heat shock ; Heat-Shock Response ; Histology ; Life Sciences ; Morphology ; Mycoplasma hyopneumoniae ; Mycoplasma hyopneumoniae - genetics ; Mycoplasma hyopneumoniae - growth & development ; Original Article ; Oxidative Stress ; Promoter Regions, Genetic ; Promoters ; Real-Time Polymerase Chain Reaction ; Regulatory sequences ; Regulatory Sequences, Nucleic Acid ; Repetitive Sequences, Nucleic Acid ; Terminator Regions, Genetic ; Transcription ; Transcription, Genetic</subject><ispartof>Molecular biology reports, 2022, Vol.49 (1), p.139-147</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Nature B.V.</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-1aa66e646dc79d09aadaadd9d91a95d0403f31b4a3940cd203456878f44aa3</citedby><cites>FETCH-LOGICAL-c375t-1aa66e646dc79d09aadaadd9d91a95d0403f31b4a3940cd203456878f44aa3</cites><orcidid>0000-0001-7972-7752 ; 0000-0003-0246-4557 ; 0000-0002-7187-2506 ; 0000-0001-7282-6117</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-021-06851-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-021-06851-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34676505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Merker Breyer, Gabriela</creatorcontrib><creatorcontrib>Malvessi Cattani, Amanda</creatorcontrib><creatorcontrib>Silveira Schrank, Irene</creatorcontrib><creatorcontrib>Maboni Siqueira, Franciele</creatorcontrib><title>The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
The comprehension of genome organization and gene modulation is essential for understanding pathogens’ infection mechanisms.
Mycoplasma hyopneumoniae
7448 genome is organized in transcriptional units (TUs), which are flanked by regulatory elements such as putative promoters, terminators and repetitive sequences. Yet the relationship between the presence of these elements and bacterial responses during stress conditions remains unclear. Thus, in this study, in silico and RT-qPCR analyses were associated to determine the effect of regulatory elements in gene expression regulation upon heat shock and oxidative stress conditions.
Methods and results
Thirteen TU’s organizational profiles were found based on promoters and terminators distribution. Differential expression in genes sharing the same TUs was observed, suggesting the activity of internal regulatory elements. Moreover, 88.8% of tested genes were differentially expressed under oxidative stress in comparison to the control condition, being 81.3% of them surrounded by their own regulatory elements. Similarly, under heat shock, 44.4% of the genes showed regulation when compared to control condition, being 75.0% of them surrounded by their own regulatory elements.
Conclusions
Altogether, this data suggests the activity of internal regulatory elements in gene modulation of
M. hyopneumoniae
7448 transcription.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene regulation</subject><subject>Genomes</subject><subject>Heat shock</subject><subject>Heat-Shock Response</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Morphology</subject><subject>Mycoplasma hyopneumoniae</subject><subject>Mycoplasma hyopneumoniae - genetics</subject><subject>Mycoplasma hyopneumoniae - growth & development</subject><subject>Original Article</subject><subject>Oxidative Stress</subject><subject>Promoter Regions, Genetic</subject><subject>Promoters</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regulatory sequences</subject><subject>Regulatory Sequences, Nucleic Acid</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription</subject><subject>Transcription, Genetic</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcuKFTEQhoMoznH0BVxIwI2b1krn1r2UwRuMCDL7UNOpntNjd9ImafE8gO9tjmdUcGEoyKK--kPqY-ypgJcCwL7KQoCUDbSiAdNp0dh7bCe0lY3qbXef7UCCaFTtnLFHOd8CgBJWP2RnUhlrNOgd-3G1Jz6Fcd4oDMTjyBPdbDOWmA6cZloolMxj4B8PQ1xnzAvy_SGugbYlhgmJW6U6XhKGPKRpLVMMONeQvMaQifstTeGGx--TxzJ9I55L7WWOwfM9YeF5H4cvj9mDEedMT-7uc_b57Zuri_fN5ad3Hy5eXzaDtLo0AtEYMsr4wfYeekRfy_e-F9hrDwrkKMW1QtkrGHwLUmnT2W5UClGesxen0DXFrxvl4pYpDzTPGChu2bW6U0raeir6_B_0Nm6pfqxSRnSyaw0cqfZEDSnmnGh0a5oWTAcnwB0NuZMhVw25X4bccejZXfR2vZD_M_JbSQXkCcjrcXWU_r79n9if9WieEA</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Merker Breyer, Gabriela</creator><creator>Malvessi Cattani, Amanda</creator><creator>Silveira Schrank, Irene</creator><creator>Maboni Siqueira, Franciele</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7972-7752</orcidid><orcidid>https://orcid.org/0000-0003-0246-4557</orcidid><orcidid>https://orcid.org/0000-0002-7187-2506</orcidid><orcidid>https://orcid.org/0000-0001-7282-6117</orcidid></search><sort><creationdate>2022</creationdate><title>The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock</title><author>Merker Breyer, Gabriela ; Malvessi Cattani, Amanda ; Silveira Schrank, Irene ; Maboni Siqueira, Franciele</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-1aa66e646dc79d09aadaadd9d91a95d0403f31b4a3940cd203456878f44aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene regulation</topic><topic>Genomes</topic><topic>Heat shock</topic><topic>Heat-Shock Response</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Morphology</topic><topic>Mycoplasma hyopneumoniae</topic><topic>Mycoplasma hyopneumoniae - genetics</topic><topic>Mycoplasma hyopneumoniae - growth & development</topic><topic>Original Article</topic><topic>Oxidative Stress</topic><topic>Promoter Regions, Genetic</topic><topic>Promoters</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regulatory sequences</topic><topic>Regulatory Sequences, Nucleic Acid</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Terminator Regions, Genetic</topic><topic>Transcription</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Merker Breyer, Gabriela</creatorcontrib><creatorcontrib>Malvessi Cattani, Amanda</creatorcontrib><creatorcontrib>Silveira Schrank, Irene</creatorcontrib><creatorcontrib>Maboni Siqueira, Franciele</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Merker Breyer, Gabriela</au><au>Malvessi Cattani, Amanda</au><au>Silveira Schrank, Irene</au><au>Maboni Siqueira, Franciele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022</date><risdate>2022</risdate><volume>49</volume><issue>1</issue><spage>139</spage><epage>147</epage><pages>139-147</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
The comprehension of genome organization and gene modulation is essential for understanding pathogens’ infection mechanisms.
Mycoplasma hyopneumoniae
7448 genome is organized in transcriptional units (TUs), which are flanked by regulatory elements such as putative promoters, terminators and repetitive sequences. Yet the relationship between the presence of these elements and bacterial responses during stress conditions remains unclear. Thus, in this study, in silico and RT-qPCR analyses were associated to determine the effect of regulatory elements in gene expression regulation upon heat shock and oxidative stress conditions.
Methods and results
Thirteen TU’s organizational profiles were found based on promoters and terminators distribution. Differential expression in genes sharing the same TUs was observed, suggesting the activity of internal regulatory elements. Moreover, 88.8% of tested genes were differentially expressed under oxidative stress in comparison to the control condition, being 81.3% of them surrounded by their own regulatory elements. Similarly, under heat shock, 44.4% of the genes showed regulation when compared to control condition, being 75.0% of them surrounded by their own regulatory elements.
Conclusions
Altogether, this data suggests the activity of internal regulatory elements in gene modulation of
M. hyopneumoniae
7448 transcription.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>34676505</pmid><doi>10.1007/s11033-021-06851-7</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7972-7752</orcidid><orcidid>https://orcid.org/0000-0003-0246-4557</orcidid><orcidid>https://orcid.org/0000-0002-7187-2506</orcidid><orcidid>https://orcid.org/0000-0001-7282-6117</orcidid></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Bacterial Proteins - genetics Biomedical and Life Sciences Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Bacterial Gene regulation Genomes Heat shock Heat-Shock Response Histology Life Sciences Morphology Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae - genetics Mycoplasma hyopneumoniae - growth & development Original Article Oxidative Stress Promoter Regions, Genetic Promoters Real-Time Polymerase Chain Reaction Regulatory sequences Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Terminator Regions, Genetic Transcription Transcription, Genetic |
| title | The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock |
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