Engineering of cis-Element in Saccharomyces cerevisiae for Efficient Accumulation of Value-Added Compound Squalene via Downregulation of the Downstream Metabolic Flux

Engineering of cis-Element in Saccharomyces cerevisiae for Efficient Accumulation of Value-Added Compound Squalene via Downregulation of the Downstream Metabolic Flux

Transcriptional downregulation is widely used for metabolic flux control. Here, marO, a cis-element of Escherichia coli mar operator, was explored to engineer promoters of Saccharomyces cerevisiae for downregulation. First, the ADH1 promoter (P ADH1 ) and its enhanced variant P UADH1 were engineered...

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Veröffentlicht in:Journal of agricultural and food chemistry 2021-10, Vol.69 (42), p.12474-12484
Hauptverfasser: Zhou, Chenyao, Li, Mingjie, Lu, Surui, Cheng, Yanfei, Guo, Xuena, He, Xiaoxian, Wang, Zhaoyue, He, Xiu-Ping
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container_end_page 12484
container_issue 42
container_start_page 12474
container_title Journal of agricultural and food chemistry
container_volume 69
creator Zhou, Chenyao
Li, Mingjie
Lu, Surui
Cheng, Yanfei
Guo, Xuena
He, Xiaoxian
Wang, Zhaoyue
He, Xiu-Ping
description Transcriptional downregulation is widely used for metabolic flux control. Here, marO, a cis-element of Escherichia coli mar operator, was explored to engineer promoters of Saccharomyces cerevisiae for downregulation. First, the ADH1 promoter (P ADH1 ) and its enhanced variant P UADH1 were engineered by insertion of marO into different sites, which resulted in decrease in both gfp5 transcription and GFP fluorescence intensity to various degrees. Then, marO was applied to engineer the native ERG1 and ERG11 promoters due to their importance for accumulation of value-added intermediates squalene and lanosterol. Elevated squalene content (4.9-fold) or lanosterol content (4.8-fold) and 91 or 28% decrease in ergosterol content resulted from the marO-engineered promoter P ERG1(M5) or P ERG11(M3) , respectively, indicating the validity of the marO-engineered promoters in metabolic flux control. Furthermore, squalene production of 3.53 g/L from cane molasses, a cheap and bulk substrate, suggested the cost-effective and promising potential for squalene production.
doi_str_mv 10.1021/acs.jafc.1c04978
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title Engineering of cis-Element in Saccharomyces cerevisiae for Efficient Accumulation of Value-Added Compound Squalene via Downregulation of the Downstream Metabolic Flux
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