Determination of proteinaceous free amino acids by gas chromatography
A method was developed for determination of proteinaceous free amino acids by gas chromatography-mass spectrometry (GC-MS). The guanidino group of arginine in amino acid samples was modified with 1,2-cyclohexanedione at room temperature under basic conditions, and all amino acids were directly deriv...
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| Veröffentlicht in: | Analytical biochemistry 2021-11, Vol.633, p.114423-114423, Article 114423 |
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| container_title | Analytical biochemistry |
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| creator | Ichihara, Ken'ichi Kohsaka, Chihiro Yamamoto, Yoshihiro |
| description | A method was developed for determination of proteinaceous free amino acids by gas chromatography-mass spectrometry (GC-MS). The guanidino group of arginine in amino acid samples was modified with 1,2-cyclohexanedione at room temperature under basic conditions, and all amino acids were directly derivatized with isobutyl chloroformate. The amino acid derivatives formed were analyzed by GC-MS. The method developed was applied successfully for the determination of amino acids in the Japanese alcoholic beverage, sake.
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•Arginine was derivatized to a stable volatile compound at room temperatures for gas chromatography.•The two sequential reactions proceeded under aqueous conditions.•Free proteinaceous amino acids in an alcoholic beverage could be determined by gas chromatography-mass spectrometry. |
| doi_str_mv | 10.1016/j.ab.2021.114423 |
| format | Article |
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•Arginine was derivatized to a stable volatile compound at room temperatures for gas chromatography.•The two sequential reactions proceeded under aqueous conditions.•Free proteinaceous amino acids in an alcoholic beverage could be determined by gas chromatography-mass spectrometry.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2021.114423</identifier><identifier>PMID: 34662572</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>1,2-Cyclohexanedione ; Amino Acids - analysis ; Arginine ; Ethanol - chemistry ; Formates - chemistry ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Guanidino group ; Japan ; Sake ; Wine - analysis</subject><ispartof>Analytical biochemistry, 2021-11, Vol.633, p.114423-114423, Article 114423</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-41a0437a6750315422540db8479ac1a9ccdd6acc4150270b0eb2d1f8fa9b0e283</citedby><cites>FETCH-LOGICAL-c350t-41a0437a6750315422540db8479ac1a9ccdd6acc4150270b0eb2d1f8fa9b0e283</cites><orcidid>0000-0002-4511-6096</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2021.114423$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34662572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichihara, Ken'ichi</creatorcontrib><creatorcontrib>Kohsaka, Chihiro</creatorcontrib><creatorcontrib>Yamamoto, Yoshihiro</creatorcontrib><title>Determination of proteinaceous free amino acids by gas chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A method was developed for determination of proteinaceous free amino acids by gas chromatography-mass spectrometry (GC-MS). The guanidino group of arginine in amino acid samples was modified with 1,2-cyclohexanedione at room temperature under basic conditions, and all amino acids were directly derivatized with isobutyl chloroformate. The amino acid derivatives formed were analyzed by GC-MS. The method developed was applied successfully for the determination of amino acids in the Japanese alcoholic beverage, sake.
[Display omitted]
•Arginine was derivatized to a stable volatile compound at room temperatures for gas chromatography.•The two sequential reactions proceeded under aqueous conditions.•Free proteinaceous amino acids in an alcoholic beverage could be determined by gas chromatography-mass spectrometry.</description><subject>1,2-Cyclohexanedione</subject><subject>Amino Acids - analysis</subject><subject>Arginine</subject><subject>Ethanol - chemistry</subject><subject>Formates - chemistry</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Guanidino group</subject><subject>Japan</subject><subject>Sake</subject><subject>Wine - analysis</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1PwzAMxSMEYmNw54R65NLhfDRtuSEYH9IkLnCO3NTdMq3LSDqk_fd06uDGybb889PzY-yaw5QD13erKVZTAYJPOVdKyBM25lDqFCSUp2wMADIVusxH7CLGFUBPZfqcjaTSWmS5GLPZE3UUWrfBzvlN4ptkG3xH_WzJ72LSBKIE-71P0Lo6JtU-WWBM7DL4Fju_CLhd7i_ZWYPrSFfHOmGfz7OPx9d0_v7y9vgwT63MoEsVR1AyR51nIHmmhMgU1FWh8hItx9LautZoreIZiBwqoErUvCkaLPteFHLCbgfd3uTXjmJnWhctrde4Obg1IiukUkWuDygMqA0-xkCN2QbXYtgbDuYQnlkZrMwhPDOE15_cHNV3VUv138FvWj1wPwDU__jtKJhoHW0s1S6Q7Uzt3f_qP1gufZ8</recordid><startdate>20211115</startdate><enddate>20211115</enddate><creator>Ichihara, Ken'ichi</creator><creator>Kohsaka, Chihiro</creator><creator>Yamamoto, Yoshihiro</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4511-6096</orcidid></search><sort><creationdate>20211115</creationdate><title>Determination of proteinaceous free amino acids by gas chromatography</title><author>Ichihara, Ken'ichi ; Kohsaka, Chihiro ; Yamamoto, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-41a0437a6750315422540db8479ac1a9ccdd6acc4150270b0eb2d1f8fa9b0e283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>1,2-Cyclohexanedione</topic><topic>Amino Acids - analysis</topic><topic>Arginine</topic><topic>Ethanol - chemistry</topic><topic>Formates - chemistry</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Guanidino group</topic><topic>Japan</topic><topic>Sake</topic><topic>Wine - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichihara, Ken'ichi</creatorcontrib><creatorcontrib>Kohsaka, Chihiro</creatorcontrib><creatorcontrib>Yamamoto, Yoshihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichihara, Ken'ichi</au><au>Kohsaka, Chihiro</au><au>Yamamoto, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of proteinaceous free amino acids by gas chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2021-11-15</date><risdate>2021</risdate><volume>633</volume><spage>114423</spage><epage>114423</epage><pages>114423-114423</pages><artnum>114423</artnum><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A method was developed for determination of proteinaceous free amino acids by gas chromatography-mass spectrometry (GC-MS). The guanidino group of arginine in amino acid samples was modified with 1,2-cyclohexanedione at room temperature under basic conditions, and all amino acids were directly derivatized with isobutyl chloroformate. The amino acid derivatives formed were analyzed by GC-MS. The method developed was applied successfully for the determination of amino acids in the Japanese alcoholic beverage, sake.
[Display omitted]
•Arginine was derivatized to a stable volatile compound at room temperatures for gas chromatography.•The two sequential reactions proceeded under aqueous conditions.•Free proteinaceous amino acids in an alcoholic beverage could be determined by gas chromatography-mass spectrometry.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>34662572</pmid><doi>10.1016/j.ab.2021.114423</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-4511-6096</orcidid></addata></record> |
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| ispartof | Analytical biochemistry, 2021-11, Vol.633, p.114423-114423, Article 114423 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 1,2-Cyclohexanedione Amino Acids - analysis Arginine Ethanol - chemistry Formates - chemistry Gas chromatography Gas Chromatography-Mass Spectrometry Guanidino group Japan Sake Wine - analysis |
| title | Determination of proteinaceous free amino acids by gas chromatography |
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