Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages
By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity‐related indicators including lipopol...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2022-10, Vol.69 (5), p.2091-2101 |
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| creator | Wang, Hui‐Yun Chen, Xiao‐chun Yan, Zhi‐han Tu, Fan He, Tian Gopinath, Subash C. B. Rui, Xiao‐hong Cao, Fu‐tao |
| description | By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity‐related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP‐1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP‐1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy‐related genes, while HNP‐1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP‐1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP‐1. All cytokines in the peptide group were significantly higher. HNP‐1 promotes immune sterilization by reducing the virulence of multidrug‐resistant K. pneumoniae and increasing the ability of macrophages.
Screening of antimicrobial peptides against Klebsiella. |
| doi_str_mv | 10.1002/bab.2270 |
| format | Article |
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Screening of antimicrobial peptides against Klebsiella.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.2270</identifier><language>eng</language><publisher>Calgary: Wiley Subscription Services, Inc</publisher><subject>Antibacterial activity ; Antiinfectives and antibacterials ; Antimicrobial peptides ; Autophagy ; Biocompatibility ; Cytokines ; Cytotoxicity ; drug resistant ; Endocytosis ; Genes ; human neutrophil peptide 1 ; immune sterilization ; Infections ; Inoculation ; Klebsiella ; Klebsiella pneumoniae ; Leukocytes (neutrophilic) ; Lipopolysaccharides ; Macrophages ; Membrane permeability ; Membrane proteins ; Membranes ; Multidrug resistant organisms ; Neutrophils ; Outer membrane proteins ; Oxidative stress ; Peptides ; Permeability ; Pneumoconiosis ; Polysaccharides ; Pp65 protein ; Proteins ; Sterilization ; Survival ; Toxicity ; Virulence</subject><ispartof>Biotechnology and applied biochemistry, 2022-10, Vol.69 (5), p.2091-2101</ispartof><rights>2021 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>2022 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3260-2b243df6ae06f654ebc6c1106b034942b06ae903dc3e86c21d0a75640f056cb3</citedby><cites>FETCH-LOGICAL-c3260-2b243df6ae06f654ebc6c1106b034942b06ae903dc3e86c21d0a75640f056cb3</cites><orcidid>0000-0002-8347-4687 ; 0000-0002-4078-3703</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.2270$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.2270$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids></links><search><creatorcontrib>Wang, Hui‐Yun</creatorcontrib><creatorcontrib>Chen, Xiao‐chun</creatorcontrib><creatorcontrib>Yan, Zhi‐han</creatorcontrib><creatorcontrib>Tu, Fan</creatorcontrib><creatorcontrib>He, Tian</creatorcontrib><creatorcontrib>Gopinath, Subash C. B.</creatorcontrib><creatorcontrib>Rui, Xiao‐hong</creatorcontrib><creatorcontrib>Cao, Fu‐tao</creatorcontrib><title>Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages</title><title>Biotechnology and applied biochemistry</title><description>By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity‐related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP‐1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP‐1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy‐related genes, while HNP‐1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP‐1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP‐1. All cytokines in the peptide group were significantly higher. HNP‐1 promotes immune sterilization by reducing the virulence of multidrug‐resistant K. pneumoniae and increasing the ability of macrophages.
Screening of antimicrobial peptides against Klebsiella.</description><subject>Antibacterial activity</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial peptides</subject><subject>Autophagy</subject><subject>Biocompatibility</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>drug resistant</subject><subject>Endocytosis</subject><subject>Genes</subject><subject>human neutrophil peptide 1</subject><subject>immune sterilization</subject><subject>Infections</subject><subject>Inoculation</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lipopolysaccharides</subject><subject>Macrophages</subject><subject>Membrane permeability</subject><subject>Membrane proteins</subject><subject>Membranes</subject><subject>Multidrug resistant organisms</subject><subject>Neutrophils</subject><subject>Outer membrane proteins</subject><subject>Oxidative stress</subject><subject>Peptides</subject><subject>Permeability</subject><subject>Pneumoconiosis</subject><subject>Polysaccharides</subject><subject>Pp65 protein</subject><subject>Proteins</subject><subject>Sterilization</subject><subject>Survival</subject><subject>Toxicity</subject><subject>Virulence</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kc1qFTEYhoMoeDwKXkLAjZupX34mM2fZFrVioZvuhyTzzWlKJjPmp3JceQnei3fklZjTWgTBVSB5eL4330vIawYnDIC_M9qccN7BE7JhsoOm76R8SjbQ920jWyaekxcp3QJA3_V8Q35elFkHGrDkuKw3ztMV1-xGpIyucZmXjIm6eS4BacoYnXffdHZLoC7QO3e3UHOgEcdiXdjTfIP1MhaPwSJdJjoXX2Wx7H99_xExuZR1yPSzR5Mceq_pWifPS3AaqQ5jldqIOj26tKnz8uHepO0xoN5jekmeTdonfPXn3JLrD--vzy-ay6uPn85PLxsruIKGGy7FOCmNoCbVSjRWWcZAGRByJ7mB-rQDMVqBvbKcjaC7VkmYoFXWiC15-6Cte_hSMOVhdskeUwdcShp42wspu64udUve_IPeLiWGGm7gHd_JVrSg_grrT1KKOA1rdLOOh4HBcCxvqOUNx_Iq2jygX53Hw3-54ez07J7_DbsMn8A</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Wang, Hui‐Yun</creator><creator>Chen, Xiao‐chun</creator><creator>Yan, Zhi‐han</creator><creator>Tu, Fan</creator><creator>He, Tian</creator><creator>Gopinath, Subash C. B.</creator><creator>Rui, Xiao‐hong</creator><creator>Cao, Fu‐tao</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8347-4687</orcidid><orcidid>https://orcid.org/0000-0002-4078-3703</orcidid></search><sort><creationdate>202210</creationdate><title>Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages</title><author>Wang, Hui‐Yun ; Chen, Xiao‐chun ; Yan, Zhi‐han ; Tu, Fan ; He, Tian ; Gopinath, Subash C. 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B.</au><au>Rui, Xiao‐hong</au><au>Cao, Fu‐tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><date>2022-10</date><risdate>2022</risdate><volume>69</volume><issue>5</issue><spage>2091</spage><epage>2101</epage><pages>2091-2101</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><abstract>By studying the expression in patients and cell modeling in vitro, antimicrobial peptides for Klebsiella were screened. Killing curve and membrane permeability experiments are used to study the antibacterial effect of antimicrobial peptides in vitro. Cytotoxicity‐related indicators including lipopolysaccharide (LPS), capsule polysaccharide (CPS), and outer membrane protein expression were measured. Intranasal inoculation of pneumoconiosis was used to construct a mouse infection model, and the survival rate and cytokine expression level were tested. Human neutrophil peptide 1 (HNP‐1) showed a significant antibacterial effect, which improved the permeability of the outer membrane of K. pneumoniae. Moreover, HNP‐1 decreased LPS, CPS content, and outer membrane proteins. K. pneumoniae infection decreased antimicrobial peptide, oxidative stress, and autophagy‐related genes, while HNP‐1 increased these genes. After coculture with macrophages, the endocytosis of macrophages is enhanced and the bacterial load is greater in the K. pneumoniae + peptide group. Besides, higher levels of pp38 and pp65 in the K. pneumoniae + peptide group. HNP‐1 rescued the cytotoxicity induced by K. pneumoniae. The survival rate is significantly improved after K. pneumoniae is treated by HNP‐1. All cytokines in the peptide group were significantly higher. HNP‐1 promotes immune sterilization by reducing the virulence of multidrug‐resistant K. pneumoniae and increasing the ability of macrophages.
Screening of antimicrobial peptides against Klebsiella.</abstract><cop>Calgary</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/bab.2270</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-8347-4687</orcidid><orcidid>https://orcid.org/0000-0002-4078-3703</orcidid></addata></record> |
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| ispartof | Biotechnology and applied biochemistry, 2022-10, Vol.69 (5), p.2091-2101 |
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| subjects | Antibacterial activity Antiinfectives and antibacterials Antimicrobial peptides Autophagy Biocompatibility Cytokines Cytotoxicity drug resistant Endocytosis Genes human neutrophil peptide 1 immune sterilization Infections Inoculation Klebsiella Klebsiella pneumoniae Leukocytes (neutrophilic) Lipopolysaccharides Macrophages Membrane permeability Membrane proteins Membranes Multidrug resistant organisms Neutrophils Outer membrane proteins Oxidative stress Peptides Permeability Pneumoconiosis Polysaccharides Pp65 protein Proteins Sterilization Survival Toxicity Virulence |
| title | Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug‐resistant Klebsiella pneumoniae and increasing the ability of macrophages |
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