Synergistic impact of platelet rich plasma-heparin sulfate with hydroxyapatite/zirconia on the osteoblast differentiation potential of adipose-derived mesenchymal stem cells
3D porous hydroxyapatite (HA) has been reinforced by zirconia (ZrO 2 ) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by f...
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| Veröffentlicht in: | Cell and tissue banking 2022-12, Vol.23 (4), p.669-683 |
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| creator | latifi, Mona Sani, Mahsa Salmannejad, Mahin Kabir-Salmani, Maryam Babakhanzadeh Bavanati, Hanieh Talaei-Khozani, Tahereh |
| description | 3D porous hydroxyapatite (HA) has been reinforced by zirconia (ZrO
2
) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by flow cytometry for CD (cluster of differentiation) 44, CD105, CD106, CD34 and CD144, along with checking the multipotency by differentiation into the adipocytes and osteoblasts. Then, they were cultured on the scaffold treated with and without osteogenic media on days 7, 14 and 21. Electron micrograph and PKH staining show that the ADMSCs have a fusiform phenotype in the absence of osteogenic induction. Cell viability assay shows a higher number of the viable cells on the PRP-containing scaffolds than PRP-free scaffolds on day 7. Colorimetric evaluation, quantitative RT-PCR and immunocytochemistry demonstrate that PRP and HS significantly elevate the alkaline phosphatase enzyme activity and also accelerate the production of both early and mid-osteogenic markers, including collagen I and osteopontin expression with and without osteogenic conditions. The PRP-HS also accelerates the expression of the late osteogenic marker, osteocalcin, in both mRNA and protein level expression with a peak on day 21. In conclusion, supplementation of HA/ZrO
2
with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO
2
/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts. |
| doi_str_mv | 10.1007/s10561-021-09966-0 |
| format | Article |
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2
) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by flow cytometry for CD (cluster of differentiation) 44, CD105, CD106, CD34 and CD144, along with checking the multipotency by differentiation into the adipocytes and osteoblasts. Then, they were cultured on the scaffold treated with and without osteogenic media on days 7, 14 and 21. Electron micrograph and PKH staining show that the ADMSCs have a fusiform phenotype in the absence of osteogenic induction. Cell viability assay shows a higher number of the viable cells on the PRP-containing scaffolds than PRP-free scaffolds on day 7. Colorimetric evaluation, quantitative RT-PCR and immunocytochemistry demonstrate that PRP and HS significantly elevate the alkaline phosphatase enzyme activity and also accelerate the production of both early and mid-osteogenic markers, including collagen I and osteopontin expression with and without osteogenic conditions. The PRP-HS also accelerates the expression of the late osteogenic marker, osteocalcin, in both mRNA and protein level expression with a peak on day 21. In conclusion, supplementation of HA/ZrO
2
with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO
2
/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-021-09966-0</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adipocytes ; Alkaline phosphatase ; Anticoagulants ; Biomedical and Life Sciences ; Biomedicine ; CD105 antigen ; CD34 antigen ; Cell Biology ; Cell differentiation ; Collagen (type I) ; Colorimetry ; Enzymatic activity ; Flow cytometry ; Gene expression ; Growth factors ; Heparin ; Hydroxyapatite ; Immunocytochemistry ; Life Sciences ; Mesenchymal stem cells ; Microenvironments ; mRNA ; Osteoblastogenesis ; Osteoblasts ; Osteocalcin ; Osteoconduction ; Osteopontin ; Phenotypes ; Platelets ; Stem cells ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2022-12, Vol.23 (4), p.669-683</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-f43b6373158a77a9fb672bacd6a81ad59bc38ba8891b3396ab0999abdb5e8f253</citedby><cites>FETCH-LOGICAL-c352t-f43b6373158a77a9fb672bacd6a81ad59bc38ba8891b3396ab0999abdb5e8f253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10561-021-09966-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10561-021-09966-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>latifi, Mona</creatorcontrib><creatorcontrib>Sani, Mahsa</creatorcontrib><creatorcontrib>Salmannejad, Mahin</creatorcontrib><creatorcontrib>Kabir-Salmani, Maryam</creatorcontrib><creatorcontrib>Babakhanzadeh Bavanati, Hanieh</creatorcontrib><creatorcontrib>Talaei-Khozani, Tahereh</creatorcontrib><title>Synergistic impact of platelet rich plasma-heparin sulfate with hydroxyapatite/zirconia on the osteoblast differentiation potential of adipose-derived mesenchymal stem cells</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><description>3D porous hydroxyapatite (HA) has been reinforced by zirconia (ZrO
2
) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by flow cytometry for CD (cluster of differentiation) 44, CD105, CD106, CD34 and CD144, along with checking the multipotency by differentiation into the adipocytes and osteoblasts. Then, they were cultured on the scaffold treated with and without osteogenic media on days 7, 14 and 21. Electron micrograph and PKH staining show that the ADMSCs have a fusiform phenotype in the absence of osteogenic induction. Cell viability assay shows a higher number of the viable cells on the PRP-containing scaffolds than PRP-free scaffolds on day 7. Colorimetric evaluation, quantitative RT-PCR and immunocytochemistry demonstrate that PRP and HS significantly elevate the alkaline phosphatase enzyme activity and also accelerate the production of both early and mid-osteogenic markers, including collagen I and osteopontin expression with and without osteogenic conditions. The PRP-HS also accelerates the expression of the late osteogenic marker, osteocalcin, in both mRNA and protein level expression with a peak on day 21. In conclusion, supplementation of HA/ZrO
2
with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO
2
/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts.</description><subject>Adipocytes</subject><subject>Alkaline phosphatase</subject><subject>Anticoagulants</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>CD105 antigen</subject><subject>CD34 antigen</subject><subject>Cell Biology</subject><subject>Cell differentiation</subject><subject>Collagen (type I)</subject><subject>Colorimetry</subject><subject>Enzymatic activity</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Heparin</subject><subject>Hydroxyapatite</subject><subject>Immunocytochemistry</subject><subject>Life Sciences</subject><subject>Mesenchymal stem cells</subject><subject>Microenvironments</subject><subject>mRNA</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteocalcin</subject><subject>Osteoconduction</subject><subject>Osteopontin</subject><subject>Phenotypes</subject><subject>Platelets</subject><subject>Stem cells</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9Uctu1TAQjRBIlAs_wMoSGzahfiSxs0QVL6kSi5a1NXbGjavEDrYvEP6Jf8S3FwmJBYuRZ3QeHs1pmpeMvmGUysvMaD-wlvJa4zgMLX3UXLBeinZQrHtce6HGdhRCPG2e5XxPKaeSi4vm180eMN35XLwlft3AFhId2RYouGAhydv5NOUV2hk3SD6QfFxchcl3X2Yy71OKP3bYoPiClz99sjF4IDGQMiOJuWA0VV_I5J3DhKH4Sq3wFsvDsJw-hMlvMWM7YfLfcCIrZgx23tcKV4uVWFyW_Lx54mDJ-OLPe2i-vH93e_Wxvf784dPV2-vWip6X1nXCDEIK1iuQEkZnBskN2GkAxWDqR2OFMqDUyIwQ4wCmHm0EM5keleO9ODSvz75bil-PmItefT5tAAHjMWveK9F1g6oHPTSv_qHex2MKdTvNpZCKStbxyuJnlk0x54ROb8mvkHbNqD4lqM8J6pqgfkhQ0yoSZ1Gu5HCH6a_1f1S_Ad8jpBU</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>latifi, Mona</creator><creator>Sani, Mahsa</creator><creator>Salmannejad, Mahin</creator><creator>Kabir-Salmani, Maryam</creator><creator>Babakhanzadeh Bavanati, Hanieh</creator><creator>Talaei-Khozani, Tahereh</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20221201</creationdate><title>Synergistic impact of platelet rich plasma-heparin sulfate with hydroxyapatite/zirconia on the osteoblast differentiation potential of adipose-derived mesenchymal stem cells</title><author>latifi, Mona ; 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2
) coating and impregnation with a combination of platelet rich plasma (PRP) as a source of growth factors (GFs) and Heparin sulfate (HS) to sustain the release of GFs. Adipose mesenchymal stem cells (ADMSCs) were characterized by flow cytometry for CD (cluster of differentiation) 44, CD105, CD106, CD34 and CD144, along with checking the multipotency by differentiation into the adipocytes and osteoblasts. Then, they were cultured on the scaffold treated with and without osteogenic media on days 7, 14 and 21. Electron micrograph and PKH staining show that the ADMSCs have a fusiform phenotype in the absence of osteogenic induction. Cell viability assay shows a higher number of the viable cells on the PRP-containing scaffolds than PRP-free scaffolds on day 7. Colorimetric evaluation, quantitative RT-PCR and immunocytochemistry demonstrate that PRP and HS significantly elevate the alkaline phosphatase enzyme activity and also accelerate the production of both early and mid-osteogenic markers, including collagen I and osteopontin expression with and without osteogenic conditions. The PRP-HS also accelerates the expression of the late osteogenic marker, osteocalcin, in both mRNA and protein level expression with a peak on day 21. In conclusion, supplementation of HA/ZrO
2
with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO
2
/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10561-021-09966-0</doi><tpages>15</tpages></addata></record> |
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| identifier | ISSN: 1389-9333 |
| ispartof | Cell and tissue banking, 2022-12, Vol.23 (4), p.669-683 |
| issn | 1389-9333 1573-6814 |
| language | eng |
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| subjects | Adipocytes Alkaline phosphatase Anticoagulants Biomedical and Life Sciences Biomedicine CD105 antigen CD34 antigen Cell Biology Cell differentiation Collagen (type I) Colorimetry Enzymatic activity Flow cytometry Gene expression Growth factors Heparin Hydroxyapatite Immunocytochemistry Life Sciences Mesenchymal stem cells Microenvironments mRNA Osteoblastogenesis Osteoblasts Osteocalcin Osteoconduction Osteopontin Phenotypes Platelets Stem cells Transplant Surgery |
| title | Synergistic impact of platelet rich plasma-heparin sulfate with hydroxyapatite/zirconia on the osteoblast differentiation potential of adipose-derived mesenchymal stem cells |
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