Functional expression and extracellular secretion of Clostridium thermocellum Cel48S cellulase in Escherichia coli via the signal recognition particle-dependent translocation pathway

•Cel48S plays crucial roles in the hydrolysis of crystalline cellulose.•The translocation of Cel48S across the outer membrane enabled its functional expression.•Extracellular Cel48S secretion was enhanced via the SRP pathway.•Engineered E. coli produced 153.5 ± 2.4 U/mL of Cel48S in the culture medi...

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Veröffentlicht in:	Enzyme and microbial technology 2021-11, Vol.151, p.109918-109918, Article 109918
Hauptverfasser:	Lee, Hyun-Jae, Kang, Tae-Gu, Kim, Young-Woo, Lee, Hee-Seok, Kim, Sun-Ki
Format:	Artikel
Sprache:	eng
Schlagworte:	Cel48S Cellulase - genetics Cellulase - metabolism Clostridium thermocellum - genetics Clostridium thermocellum - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Extracellular secretion Periplasm - metabolism Signal Recognition Particle Signal recognition particle-dependent pathway
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