Ketamine induction of physiological functions alterations in Caenorhabditis elegans by chronic and multigenerational exposure and corresponding aquatic environmental risk assessment

Although ketamine (KET) has been widely detected in aquatic environments, the ecotoxicity data in aquatic invertebrates and associated risk remained unclear. This study aimed to investigate the adverse effects on benthos (Caenorhabditis elegans (C.elegans)) posed by KET from chronic (10 days) and multigenerational (four generations) exposure. Such exposure induced dose-dependent alterations on apoptosis, reactive oxygen species (ROS) induction, locomotion activity, feeding rate, chemotaxis, and brood size of nematodes, showing a cumulative damage through generations. KET posed vulva deformations and worm bags of C. elegans with a dosed-dependent increase. As a consequence, the fecundity and viability of worms would be impaired, which could eventually impact aquatic ecosystem equilibrium. Meanwhile, the bioactivation/detoxification process of xenobiotics and longevity regulating pathway induced by KET might be responsible for the physiological function disorders. Accordingly, the risk quotients (RQ) of KET in surface water in China were calculated using the 90% indicator protection concentration (C0.1) derived from multiple toxicity indicators cumulative analyses. The results would be more objective considering numerous biomarkers changes of one species in comparison with traditional method using no observed effect concentrations (NOEC) of teratogenesis. The risk in surface water in southern China was up to high level (RQ > 1), suggesting long-term monitoring was imperative. [Display omitted] •Underlying molecular toxicity mechanisms of ketamine to worms were elucidated.•Ketamine induced marked changes on physiological functions of C. elegans at low levels.•Multigenerational toxicity confirmed cumulative damages of ketamine to C. elegans.•A new risk quotient (RQ) calculative method using multiple biomarkers was proposed.•The risk levels of ketamine in surface water in Southern China were up to high level.