Estradiol modulated differentiation and dynamic growth of CD90+ spermatogonial stem cells toward Sertoli-like cells
Mouse CD90+ SSCs were enriched using the MACS technique and incubated with different doses of estradiol, ranging from 0.01 ng/mL to 500 μg/mL, for 7 days. The viability of SSCs was determined using an MTT assay. The combined effects of estradiol plus Sertoli cell differentiation medium on the orient...
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| Veröffentlicht in: | Life sciences (1973) 2021-12, Vol.286, p.120041-120041, Article 120041 |
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| creator | Sokouti Nasimi, Fatemeh Zahri, Saber Ahmadian, Shahin Bagherzadeh, Afsaneh Nazdikbin Yamchi, Nahideh Haghighi, Leila Bedate, Alberto Miranda Khalilzadeh, Balal Rahbarghazi, Reza Mahdipour, Mahdi |
| description | Mouse CD90+ SSCs were enriched using the MACS technique and incubated with different doses of estradiol, ranging from 0.01 ng/mL to 500 μg/mL, for 7 days. The viability of SSCs was determined using an MTT assay. The combined effects of estradiol plus Sertoli cell differentiation medium on the orientation of SSCs toward Sertoli-like cells were also assessed. Using immunofluorescence imaging, we monitored protein levels of Oct3/4 after being exposed to estradiol. In addition, protein levels of testosterone, TF, and ABP were measured using ELISA. The expression of Sertoli cell-specific genes such as SOX9, GATA4, FSHR, TF, and ESR-1 and -2 was monitored using real-time PCR assay, and the effects of 14-day injection of estradiol on sperm parameters and Oct3/4 positive progenitor cells in a model of mouse were determined.
Data showed that estradiol increased the viability of mouse SSCs in a dose-dependent manner compared to the control (p |
| doi_str_mv | 10.1016/j.lfs.2021.120041 |
| format | Article |
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Data showed that estradiol increased the viability of mouse SSCs in a dose-dependent manner compared to the control (p < 0.05). Along with these changes, cells displayed morphological changes and reduced Oct3/4 transcription factor levels compared to the control SSCs. 7-day incubation of SSCs with estradiol led to the up-regulation of SOX9, GATA4, FSHR, TF, and ESR-1 and -2, and levels of testosterone, TF, and ABP were increased compared to the control group (p < 0.05). The in-vivo examination noted that estradiol reduced sperm parameters coincided with morphological abnormalities (p < 0.05). Histological examination revealed pathological changes in seminiferous tubules and reduction of testicular Oct3/4+ progenitor cells.
In conclusion, estradiol treatment probably can induce Sertoli cell differentiation of SSCs while exogenous administration leads to testicular progenitor cell depletion and infertility in long term.
[Display omitted]</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2021.120041</identifier><identifier>PMID: 34637796</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>17β-Estradiol ; Abnormalities ; Adult Germline Stem Cells - drug effects ; Adult Germline Stem Cells - metabolism ; Animals ; CD90 antigen ; Cell differentiation ; Cell Differentiation - drug effects ; Depletion ; Differentiation ; Differentiation (biology) ; Enzyme-linked immunosorbent assay ; Estradiol - metabolism ; Estradiol - pharmacology ; Gene expression ; Immunofluorescence ; Infertility ; Male ; Mice ; Mice, Inbred BALB C ; Morphology ; Mouse ; Oct-4 protein ; Parameters ; Progenitor cells ; Proteins ; RNA, Messenger - genetics ; Sertoli cells ; Sertoli Cells - metabolism ; Sex hormones ; Sox9 protein ; Sperm ; Spermatogenesis - drug effects ; Spermatogenesis - physiology ; Spermatogonial stem cells ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Stem cell transplantation ; Stem cells ; Stem Cells - drug effects ; Stem Cells - metabolism ; Testes ; Testis - metabolism ; Testosterone ; Testosterone - metabolism ; Tubules</subject><ispartof>Life sciences (1973), 2021-12, Vol.286, p.120041-120041, Article 120041</ispartof><rights>2021 Elsevier Inc.</rights><rights>Copyright © 2021 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 1, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-19233711662ae31a14faad7662b3817305f818b334072527e7b78a71202d33e23</citedby><cites>FETCH-LOGICAL-c381t-19233711662ae31a14faad7662b3817305f818b334072527e7b78a71202d33e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2021.120041$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34637796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sokouti Nasimi, Fatemeh</creatorcontrib><creatorcontrib>Zahri, Saber</creatorcontrib><creatorcontrib>Ahmadian, Shahin</creatorcontrib><creatorcontrib>Bagherzadeh, Afsaneh</creatorcontrib><creatorcontrib>Nazdikbin Yamchi, Nahideh</creatorcontrib><creatorcontrib>Haghighi, Leila</creatorcontrib><creatorcontrib>Bedate, Alberto Miranda</creatorcontrib><creatorcontrib>Khalilzadeh, Balal</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Mahdipour, Mahdi</creatorcontrib><title>Estradiol modulated differentiation and dynamic growth of CD90+ spermatogonial stem cells toward Sertoli-like cells</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Mouse CD90+ SSCs were enriched using the MACS technique and incubated with different doses of estradiol, ranging from 0.01 ng/mL to 500 μg/mL, for 7 days. The viability of SSCs was determined using an MTT assay. The combined effects of estradiol plus Sertoli cell differentiation medium on the orientation of SSCs toward Sertoli-like cells were also assessed. Using immunofluorescence imaging, we monitored protein levels of Oct3/4 after being exposed to estradiol. In addition, protein levels of testosterone, TF, and ABP were measured using ELISA. The expression of Sertoli cell-specific genes such as SOX9, GATA4, FSHR, TF, and ESR-1 and -2 was monitored using real-time PCR assay, and the effects of 14-day injection of estradiol on sperm parameters and Oct3/4 positive progenitor cells in a model of mouse were determined.
Data showed that estradiol increased the viability of mouse SSCs in a dose-dependent manner compared to the control (p < 0.05). Along with these changes, cells displayed morphological changes and reduced Oct3/4 transcription factor levels compared to the control SSCs. 7-day incubation of SSCs with estradiol led to the up-regulation of SOX9, GATA4, FSHR, TF, and ESR-1 and -2, and levels of testosterone, TF, and ABP were increased compared to the control group (p < 0.05). The in-vivo examination noted that estradiol reduced sperm parameters coincided with morphological abnormalities (p < 0.05). Histological examination revealed pathological changes in seminiferous tubules and reduction of testicular Oct3/4+ progenitor cells.
In conclusion, estradiol treatment probably can induce Sertoli cell differentiation of SSCs while exogenous administration leads to testicular progenitor cell depletion and infertility in long term.
[Display omitted]</description><subject>17β-Estradiol</subject><subject>Abnormalities</subject><subject>Adult Germline Stem Cells - drug effects</subject><subject>Adult Germline Stem Cells - metabolism</subject><subject>Animals</subject><subject>CD90 antigen</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Depletion</subject><subject>Differentiation</subject><subject>Differentiation (biology)</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Estradiol - metabolism</subject><subject>Estradiol - pharmacology</subject><subject>Gene expression</subject><subject>Immunofluorescence</subject><subject>Infertility</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Morphology</subject><subject>Mouse</subject><subject>Oct-4 protein</subject><subject>Parameters</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>RNA, Messenger - genetics</subject><subject>Sertoli cells</subject><subject>Sertoli Cells - metabolism</subject><subject>Sex hormones</subject><subject>Sox9 protein</subject><subject>Sperm</subject><subject>Spermatogenesis - drug effects</subject><subject>Spermatogenesis - physiology</subject><subject>Spermatogonial stem cells</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>Testes</subject><subject>Testis - metabolism</subject><subject>Testosterone</subject><subject>Testosterone - metabolism</subject><subject>Tubules</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EotvCB-CCLHFBqrJ47NhOxKlayh-pEgfgbHnjSfHixIvtUPXb41UKBw6crPH83tPoPUJeANsCA_XmsA1j3nLGYQucsRYekQ10um-YEvCYbBjjbSM4k2fkPOcDY0xKLZ6SM9EqoXWvNiRf55Ks8zHQKbol2IKOOj-OmHAu3hYfZ2rn-nc_28kP9DbFu_KdxpHu3vXskuYjpsmWeBtnbwPNBSc6YAiZlnhnk6NfMJUYfBP8D1w3z8iT0YaMzx_eC_Lt_fXX3cfm5vOHT7urm2YQHZQGei6EBlCKWxRgoR2tdbqO-7rXgsmxg24vRMs0l1yj3uvO6poEd0IgFxfk9ep7TPHngrmYyefTBXbGuGTDZdXXUIBV9NU_6CEuaa7XGa6YVLLnSlYKVmpIMeeEozkmP9l0b4CZUyPmYGoj5tSIWRupmpcPzst-QvdX8aeCCrxdAaxR_PKYTB48zgM6n3AoxkX_H_vfJKKaCA</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Sokouti Nasimi, Fatemeh</creator><creator>Zahri, Saber</creator><creator>Ahmadian, Shahin</creator><creator>Bagherzadeh, Afsaneh</creator><creator>Nazdikbin Yamchi, Nahideh</creator><creator>Haghighi, Leila</creator><creator>Bedate, Alberto Miranda</creator><creator>Khalilzadeh, Balal</creator><creator>Rahbarghazi, Reza</creator><creator>Mahdipour, Mahdi</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20211201</creationdate><title>Estradiol modulated differentiation and dynamic growth of CD90+ spermatogonial stem cells toward Sertoli-like cells</title><author>Sokouti Nasimi, Fatemeh ; Zahri, Saber ; Ahmadian, Shahin ; Bagherzadeh, Afsaneh ; Nazdikbin Yamchi, Nahideh ; Haghighi, Leila ; Bedate, Alberto Miranda ; Khalilzadeh, Balal ; Rahbarghazi, Reza ; Mahdipour, Mahdi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-19233711662ae31a14faad7662b3817305f818b334072527e7b78a71202d33e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>17β-Estradiol</topic><topic>Abnormalities</topic><topic>Adult Germline Stem Cells - drug effects</topic><topic>Adult Germline Stem Cells - metabolism</topic><topic>Animals</topic><topic>CD90 antigen</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Depletion</topic><topic>Differentiation</topic><topic>Differentiation (biology)</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Estradiol - metabolism</topic><topic>Estradiol - pharmacology</topic><topic>Gene expression</topic><topic>Immunofluorescence</topic><topic>Infertility</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Morphology</topic><topic>Mouse</topic><topic>Oct-4 protein</topic><topic>Parameters</topic><topic>Progenitor cells</topic><topic>Proteins</topic><topic>RNA, Messenger - genetics</topic><topic>Sertoli cells</topic><topic>Sertoli Cells - metabolism</topic><topic>Sex hormones</topic><topic>Sox9 protein</topic><topic>Sperm</topic><topic>Spermatogenesis - drug effects</topic><topic>Spermatogenesis - physiology</topic><topic>Spermatogonial stem cells</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>Testes</topic><topic>Testis - metabolism</topic><topic>Testosterone</topic><topic>Testosterone - metabolism</topic><topic>Tubules</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sokouti Nasimi, Fatemeh</creatorcontrib><creatorcontrib>Zahri, Saber</creatorcontrib><creatorcontrib>Ahmadian, Shahin</creatorcontrib><creatorcontrib>Bagherzadeh, Afsaneh</creatorcontrib><creatorcontrib>Nazdikbin Yamchi, Nahideh</creatorcontrib><creatorcontrib>Haghighi, Leila</creatorcontrib><creatorcontrib>Bedate, Alberto Miranda</creatorcontrib><creatorcontrib>Khalilzadeh, Balal</creatorcontrib><creatorcontrib>Rahbarghazi, Reza</creatorcontrib><creatorcontrib>Mahdipour, Mahdi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sokouti Nasimi, Fatemeh</au><au>Zahri, Saber</au><au>Ahmadian, Shahin</au><au>Bagherzadeh, Afsaneh</au><au>Nazdikbin Yamchi, Nahideh</au><au>Haghighi, Leila</au><au>Bedate, Alberto Miranda</au><au>Khalilzadeh, Balal</au><au>Rahbarghazi, Reza</au><au>Mahdipour, Mahdi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estradiol modulated differentiation and dynamic growth of CD90+ spermatogonial stem cells toward Sertoli-like cells</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>286</volume><spage>120041</spage><epage>120041</epage><pages>120041-120041</pages><artnum>120041</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Mouse CD90+ SSCs were enriched using the MACS technique and incubated with different doses of estradiol, ranging from 0.01 ng/mL to 500 μg/mL, for 7 days. The viability of SSCs was determined using an MTT assay. The combined effects of estradiol plus Sertoli cell differentiation medium on the orientation of SSCs toward Sertoli-like cells were also assessed. Using immunofluorescence imaging, we monitored protein levels of Oct3/4 after being exposed to estradiol. In addition, protein levels of testosterone, TF, and ABP were measured using ELISA. The expression of Sertoli cell-specific genes such as SOX9, GATA4, FSHR, TF, and ESR-1 and -2 was monitored using real-time PCR assay, and the effects of 14-day injection of estradiol on sperm parameters and Oct3/4 positive progenitor cells in a model of mouse were determined.
Data showed that estradiol increased the viability of mouse SSCs in a dose-dependent manner compared to the control (p < 0.05). Along with these changes, cells displayed morphological changes and reduced Oct3/4 transcription factor levels compared to the control SSCs. 7-day incubation of SSCs with estradiol led to the up-regulation of SOX9, GATA4, FSHR, TF, and ESR-1 and -2, and levels of testosterone, TF, and ABP were increased compared to the control group (p < 0.05). The in-vivo examination noted that estradiol reduced sperm parameters coincided with morphological abnormalities (p < 0.05). Histological examination revealed pathological changes in seminiferous tubules and reduction of testicular Oct3/4+ progenitor cells.
In conclusion, estradiol treatment probably can induce Sertoli cell differentiation of SSCs while exogenous administration leads to testicular progenitor cell depletion and infertility in long term.
[Display omitted]</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>34637796</pmid><doi>10.1016/j.lfs.2021.120041</doi><tpages>1</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 17β-Estradiol Abnormalities Adult Germline Stem Cells - drug effects Adult Germline Stem Cells - metabolism Animals CD90 antigen Cell differentiation Cell Differentiation - drug effects Depletion Differentiation Differentiation (biology) Enzyme-linked immunosorbent assay Estradiol - metabolism Estradiol - pharmacology Gene expression Immunofluorescence Infertility Male Mice Mice, Inbred BALB C Morphology Mouse Oct-4 protein Parameters Progenitor cells Proteins RNA, Messenger - genetics Sertoli cells Sertoli Cells - metabolism Sex hormones Sox9 protein Sperm Spermatogenesis - drug effects Spermatogenesis - physiology Spermatogonial stem cells Spermatozoa - drug effects Spermatozoa - metabolism Stem cell transplantation Stem cells Stem Cells - drug effects Stem Cells - metabolism Testes Testis - metabolism Testosterone Testosterone - metabolism Tubules |
| title | Estradiol modulated differentiation and dynamic growth of CD90+ spermatogonial stem cells toward Sertoli-like cells |
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