Copeptin, miRNA-208, and miRNA-499 as New Biomarkers for Early Detection of Acute Coronary Syndrome
cTn and CK-MB are gold standard biomarkers for acute coronary syndrome (ACS) but are less sensitive in the first 3 h after onset of symptoms. A need thus exists for novel biomarkers for early detection of ACS. We evaluated circulating copeptin, miRNA-208, and miRNA-499 as possible biomarkers for ear...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2022-03, Vol.194 (3), p.1193-1205 |
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| creator | Gaber, Marwa A. Omar, Omnia H. M. El-Deek, Sahar E. M. Hassan, Ayman K. M. Mahmoud, Marwan S. Meki, Abdel-Raheim M. A. |
| description | cTn and CK-MB are gold standard biomarkers for acute coronary syndrome (ACS) but are less sensitive in the first 3 h after onset of symptoms. A need thus exists for novel biomarkers for early detection of ACS. We evaluated circulating copeptin, miRNA-208, and miRNA-499 as possible biomarkers for early detection of unstable angina (UA) and non-ST-segment elevation myocardial infarction (NSTEMI). Sixty-five patients with probable ACS that presented within 4 h of the onset of chest pain (23 UA and 42 NSTEMI) and 25 apparently healthy individuals were studied. Two sets of blood samples collected in the first 3 h and at 6 h after onset were analyzed for copeptin levels via ELISA and miRNA-208 and miRNA-499 expression via real-time PCR. Copeptin, miRNA-208, and miRNA-499 expression levels were significantly increased in UA and NSTEMI patients compared with controls (
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| doi_str_mv | 10.1007/s12010-021-03695-6 |
| format | Article |
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p
< 0.001) and in NSTEMT compared with UA patients (
p
< 0.001). Levels were also significantly elevated in UA and NSTEMI patients with negative cardiac troponin in the first 3 h (
p
< 0.001). ROC curves displayed AUC for prediction of ACS of 0.96 for copeptin, 0.97 for miRNA-208, and 0.97 for miRNA-499. Their combination improved AUC to 0.98. Copeptin and miRNA-208 and miRNA-499 expression are promising biomarkers for UA and NSTEMI that present in the first 3 h of pain onset. A combination of these markers with cTn may increase the accuracy of diagnosis by avoiding the gray zone of cTn as a biomarker.</description><identifier>ISSN: 0273-2289</identifier><identifier>ISSN: 1559-0291</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-021-03695-6</identifier><identifier>PMID: 34637111</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acute Coronary Syndrome - blood ; Acute Coronary Syndrome - diagnosis ; Acute coronary syndromes ; Aged ; Angina ; Angina, Unstable - blood ; Angina, Unstable - diagnosis ; Angina, Unstable - genetics ; Biochemistry ; Biomarkers ; Biomarkers - blood ; Biotechnology ; Calcium-binding protein ; Case-Control Studies ; Chemistry ; Chemistry and Materials Science ; Early Diagnosis ; Female ; Glycopeptides - blood ; Humans ; Male ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; miRNA ; Myocardial infarction ; Original Article ; Pain ; Troponin</subject><ispartof>Applied biochemistry and biotechnology, 2022-03, Vol.194 (3), p.1193-1205</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-4326e3f25234dbf75d8e5b5e6672403f181af20afcbe4afbcc4c953eb1e0ac203</citedby><cites>FETCH-LOGICAL-c375t-4326e3f25234dbf75d8e5b5e6672403f181af20afcbe4afbcc4c953eb1e0ac203</cites><orcidid>0000-0002-2171-6994</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-021-03695-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-021-03695-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34637111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaber, Marwa A.</creatorcontrib><creatorcontrib>Omar, Omnia H. M.</creatorcontrib><creatorcontrib>El-Deek, Sahar E. M.</creatorcontrib><creatorcontrib>Hassan, Ayman K. M.</creatorcontrib><creatorcontrib>Mahmoud, Marwan S.</creatorcontrib><creatorcontrib>Meki, Abdel-Raheim M. A.</creatorcontrib><title>Copeptin, miRNA-208, and miRNA-499 as New Biomarkers for Early Detection of Acute Coronary Syndrome</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>cTn and CK-MB are gold standard biomarkers for acute coronary syndrome (ACS) but are less sensitive in the first 3 h after onset of symptoms. A need thus exists for novel biomarkers for early detection of ACS. We evaluated circulating copeptin, miRNA-208, and miRNA-499 as possible biomarkers for early detection of unstable angina (UA) and non-ST-segment elevation myocardial infarction (NSTEMI). Sixty-five patients with probable ACS that presented within 4 h of the onset of chest pain (23 UA and 42 NSTEMI) and 25 apparently healthy individuals were studied. Two sets of blood samples collected in the first 3 h and at 6 h after onset were analyzed for copeptin levels via ELISA and miRNA-208 and miRNA-499 expression via real-time PCR. Copeptin, miRNA-208, and miRNA-499 expression levels were significantly increased in UA and NSTEMI patients compared with controls (
p
< 0.001) and in NSTEMT compared with UA patients (
p
< 0.001). Levels were also significantly elevated in UA and NSTEMI patients with negative cardiac troponin in the first 3 h (
p
< 0.001). ROC curves displayed AUC for prediction of ACS of 0.96 for copeptin, 0.97 for miRNA-208, and 0.97 for miRNA-499. Their combination improved AUC to 0.98. Copeptin and miRNA-208 and miRNA-499 expression are promising biomarkers for UA and NSTEMI that present in the first 3 h of pain onset. A combination of these markers with cTn may increase the accuracy of diagnosis by avoiding the gray zone of cTn as a biomarker.</description><subject>Acute Coronary Syndrome - blood</subject><subject>Acute Coronary Syndrome - diagnosis</subject><subject>Acute coronary syndromes</subject><subject>Aged</subject><subject>Angina</subject><subject>Angina, Unstable - blood</subject><subject>Angina, Unstable - diagnosis</subject><subject>Angina, Unstable - genetics</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Biotechnology</subject><subject>Calcium-binding protein</subject><subject>Case-Control Studies</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Early Diagnosis</subject><subject>Female</subject><subject>Glycopeptides - blood</subject><subject>Humans</subject><subject>Male</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Myocardial infarction</subject><subject>Original Article</subject><subject>Pain</subject><subject>Troponin</subject><issn>0273-2289</issn><issn>1559-0291</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU1vFDEMhiMEotvCH-CAInHh0GkdZzLJHJelBaSqlfg4R5mMg6bsTJZkRtX-ewK7gMSBk2X78WvLL2MvBFwIAH2ZBYKAClBUIJtWVc0jthJKtaXUisdsBahlhWjaE3aa8z2AQKP0U3Yi60ZqIcSK-U3c0W4epnM-Dh9v1xWCOedu6o9p3bbcZX5LD_zNEEeXvlHKPMTEr1za7vlbmsnPQ5x4DHztl5n4JqY4ubTnn_ZTn-JIz9iT4LaZnh_jGftyffV58766uXv3YbO-qbzUaq5qiQ3JgApl3XdBq96Q6hQ1jcYaZBBGuIDggu-odqHzvvatktQJAucR5Bl7fdDdpfh9oTzbccietls3UVyyRWUEaqOUKuirf9D7uKSpXGexQQOilkYXCg-UTzHnRMHu0lB-sLcC7E8L7MECWyywvyywTRl6eZReupH6PyO_f14AeQByaU1fKf3d_R_ZHwjXjpU</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Gaber, Marwa A.</creator><creator>Omar, Omnia H. 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M.</au><au>El-Deek, Sahar E. M.</au><au>Hassan, Ayman K. M.</au><au>Mahmoud, Marwan S.</au><au>Meki, Abdel-Raheim M. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Copeptin, miRNA-208, and miRNA-499 as New Biomarkers for Early Detection of Acute Coronary Syndrome</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>194</volume><issue>3</issue><spage>1193</spage><epage>1205</epage><pages>1193-1205</pages><issn>0273-2289</issn><issn>1559-0291</issn><eissn>1559-0291</eissn><abstract>cTn and CK-MB are gold standard biomarkers for acute coronary syndrome (ACS) but are less sensitive in the first 3 h after onset of symptoms. A need thus exists for novel biomarkers for early detection of ACS. We evaluated circulating copeptin, miRNA-208, and miRNA-499 as possible biomarkers for early detection of unstable angina (UA) and non-ST-segment elevation myocardial infarction (NSTEMI). Sixty-five patients with probable ACS that presented within 4 h of the onset of chest pain (23 UA and 42 NSTEMI) and 25 apparently healthy individuals were studied. Two sets of blood samples collected in the first 3 h and at 6 h after onset were analyzed for copeptin levels via ELISA and miRNA-208 and miRNA-499 expression via real-time PCR. Copeptin, miRNA-208, and miRNA-499 expression levels were significantly increased in UA and NSTEMI patients compared with controls (
p
< 0.001) and in NSTEMT compared with UA patients (
p
< 0.001). Levels were also significantly elevated in UA and NSTEMI patients with negative cardiac troponin in the first 3 h (
p
< 0.001). ROC curves displayed AUC for prediction of ACS of 0.96 for copeptin, 0.97 for miRNA-208, and 0.97 for miRNA-499. Their combination improved AUC to 0.98. Copeptin and miRNA-208 and miRNA-499 expression are promising biomarkers for UA and NSTEMI that present in the first 3 h of pain onset. A combination of these markers with cTn may increase the accuracy of diagnosis by avoiding the gray zone of cTn as a biomarker.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34637111</pmid><doi>10.1007/s12010-021-03695-6</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-2171-6994</orcidid></addata></record> |
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| subjects | Acute Coronary Syndrome - blood Acute Coronary Syndrome - diagnosis Acute coronary syndromes Aged Angina Angina, Unstable - blood Angina, Unstable - diagnosis Angina, Unstable - genetics Biochemistry Biomarkers Biomarkers - blood Biotechnology Calcium-binding protein Case-Control Studies Chemistry Chemistry and Materials Science Early Diagnosis Female Glycopeptides - blood Humans Male MicroRNAs - blood MicroRNAs - genetics Middle Aged miRNA Myocardial infarction Original Article Pain Troponin |
| title | Copeptin, miRNA-208, and miRNA-499 as New Biomarkers for Early Detection of Acute Coronary Syndrome |
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