Stress-induced glucocorticoids alter the Leydig cells' timing and steroidogenesis-related systems
The study aimed to analyze the time-dependent consequences of stress on gene expression responsible for diurnal endocrine Leydig cell function connecting them to the glucocorticoid-signaling. In the first 24h after the stress event, a daily variation of blood corticosterone increased, and testostero...
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Veröffentlicht in: | Molecular and cellular endocrinology 2021-12, Vol.538, p.111469-111469, Article 111469 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The study aimed to analyze the time-dependent consequences of stress on gene expression responsible for diurnal endocrine Leydig cell function connecting them to the glucocorticoid-signaling. In the first 24h after the stress event, a daily variation of blood corticosterone increased, and testosterone decreased; the testosterone/corticosterone were lowest at the end of the stress session overlapping with inhibition of Leydig cells' steroidogenesis-related genes (Nr3c1/GR, Hsd3b1/2, Star, Cyp17a1) and changed circadian activity of the clock genes (the increased Bmal1/BMAL1 and Per1/2/PER1 and decreased Cry1 and Rev-erba). The glucocorticoid-treated rats showed a similar response. The principal-component-analysis (PCA) displayed an absence of significant differences between treatments especially on Per1 and Rev-erba, the findings confirmed by the in vivo blockade of the testicular glucocorticoid receptor (GR) during stress and ex vivo treatment of the Leydig cells with hydrocortisone and GR-blocker. In summary, stressful stimuli can entrain the clock in the Leydig cells through glucocorticoid-mediated communication.
•Stress disturbs circadian clock and steroidogenesis-related genes in Leydig cells.•Stress and Dexasone provoke a similar response on clock and steroidogenesis genes.•Stress affects clock in the Leydig cells through glucocorticoid-mediated communication. |
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ISSN: | 0303-7207 1872-8057 |
DOI: | 10.1016/j.mce.2021.111469 |