Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model
Decellularized extra‐cellular matrix (ECM) has been studied as an alternative to anti‐adhesive biomaterials and cartilage acellular matrix (CAM) has been shown to inhibit postoperative adhesion in several organs. This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM‐fil...
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| Veröffentlicht in: | Journal of tissue engineering and regenerative medicine 2021-11, Vol.15 (11), p.1023-1036 |
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| creator | Shin, Young Ho Yun, Hee‐Woong Park, Suk Young Choi, Soon Jin Park, In‐Su Min, Byoung‐Hyun Kim, Jae Kwang |
| description | Decellularized extra‐cellular matrix (ECM) has been studied as an alternative to anti‐adhesive biomaterials and cartilage acellular matrix (CAM) has been shown to inhibit postoperative adhesion in several organs. This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM‐films as anti‐adhesion barriers for peripheral nerve injury. The films were successfully fabricated and showed improved physical properties such as mechanical strength, swelling ratio, and lengthened degradation period while maintaining the microstructure and chemical composition after GA crosslinking. In the in vitro study of CAM‐film, the dsDNA content met the recommended limit of decellularization and more than 70% of the major ECM components were preserved after decellularization. The adhesion and proliferation of seeded human umbilical vein endothelial cells and fibroblasts were significantly lower in CAM‐film than in control, but similar with Seprafilm. However, the CAM‐film extract did not show cytotoxicity. In the in vivo study, the peri‐neural fibrosis was thicker, adhesion score higher, and peri‐neural collagen fibers more abundant in the control group than in the CAM‐film group. The total number of myelinated axons was significantly higher in the CAM‐film group than in the control group. The inflammatory marker decreased with time in the CAM‐film group compared to that in the control group, whereas the nerve regenerative marker expression was maintained. Moreover, the ankle angles at contracture and toe‐off were higher in the CAM film‐treated rats than in the control rats. GA‐crosslinked CAM films may be used during peripheral nerve surgery to prevent peri‐neural adhesion and enhance nerve functional recovery. |
| doi_str_mv | 10.1002/term.3249 |
| format | Article |
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This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM‐films as anti‐adhesion barriers for peripheral nerve injury. The films were successfully fabricated and showed improved physical properties such as mechanical strength, swelling ratio, and lengthened degradation period while maintaining the microstructure and chemical composition after GA crosslinking. In the in vitro study of CAM‐film, the dsDNA content met the recommended limit of decellularization and more than 70% of the major ECM components were preserved after decellularization. The adhesion and proliferation of seeded human umbilical vein endothelial cells and fibroblasts were significantly lower in CAM‐film than in control, but similar with Seprafilm. However, the CAM‐film extract did not show cytotoxicity. In the in vivo study, the peri‐neural fibrosis was thicker, adhesion score higher, and peri‐neural collagen fibers more abundant in the control group than in the CAM‐film group. The total number of myelinated axons was significantly higher in the CAM‐film group than in the control group. The inflammatory marker decreased with time in the CAM‐film group compared to that in the control group, whereas the nerve regenerative marker expression was maintained. Moreover, the ankle angles at contracture and toe‐off were higher in the CAM film‐treated rats than in the control rats. GA‐crosslinked CAM films may be used during peripheral nerve surgery to prevent peri‐neural adhesion and enhance nerve functional recovery.</description><identifier>ISSN: 1932-6254</identifier><identifier>EISSN: 1932-7005</identifier><identifier>DOI: 10.1002/term.3249</identifier><identifier>PMID: 34591344</identifier><language>eng</language><publisher>England: Hindawi Limited</publisher><subject>Adhesion ; Animals ; Ankle ; Axons ; Biocompatibility ; Biomaterials ; Biomedical materials ; Cartilage ; Cartilage - chemistry ; cartilage acellular matrix ; Cell Adhesion ; Cell Death ; Cell Proliferation ; Chemical composition ; Collagen ; Collagen - metabolism ; Cross-Linking Reagents - chemistry ; Crosslinking ; Cytotoxicity ; Disease Models, Animal ; Endothelial cells ; Extracellular matrix ; Extracellular Matrix - chemistry ; extra‐cellular matrix ; Fibroblasts ; Fibrosis ; Glutaral - chemistry ; Glutaraldehyde ; Human Umbilical Vein Endothelial Cells - metabolism ; Humans ; In vivo methods and tests ; Inflammation ; Male ; Markers ; Mechanical properties ; Mice ; nerve functional recovery ; nerve injury ; Nerve Regeneration - physiology ; Organs ; peripheral nerve ; Peripheral nerves ; Physical properties ; Rats ; Rats, Sprague-Dawley ; Recovery of function ; Recovery of Function - drug effects ; Regeneration ; Regenerative medicine ; Sciatic nerve ; Sciatic Nerve - immunology ; Sciatic Nerve - injuries ; Sciatic Nerve - pathology ; Sciatic Nerve - physiopathology ; Swelling ratio ; Swine ; Tissue engineering ; Toxicity ; Umbilical vein</subject><ispartof>Journal of tissue engineering and regenerative medicine, 2021-11, Vol.15 (11), p.1023-1036</ispartof><rights>2021 John Wiley & Sons Ltd.</rights><rights>2021 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3889-b08c439ecfb9a1b81c57e23f264c3cc50d29b9fd27dd9fc431bbd4a3c10c34e13</citedby><cites>FETCH-LOGICAL-c3889-b08c439ecfb9a1b81c57e23f264c3cc50d29b9fd27dd9fc431bbd4a3c10c34e13</cites><orcidid>0000-0001-6919-5035 ; 0000-0001-5104-4634</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fterm.3249$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fterm.3249$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34591344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shin, Young Ho</creatorcontrib><creatorcontrib>Yun, Hee‐Woong</creatorcontrib><creatorcontrib>Park, Suk Young</creatorcontrib><creatorcontrib>Choi, Soon Jin</creatorcontrib><creatorcontrib>Park, In‐Su</creatorcontrib><creatorcontrib>Min, Byoung‐Hyun</creatorcontrib><creatorcontrib>Kim, Jae Kwang</creatorcontrib><title>Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model</title><title>Journal of tissue engineering and regenerative medicine</title><addtitle>J Tissue Eng Regen Med</addtitle><description>Decellularized extra‐cellular matrix (ECM) has been studied as an alternative to anti‐adhesive biomaterials and cartilage acellular matrix (CAM) has been shown to inhibit postoperative adhesion in several organs. This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM‐films as anti‐adhesion barriers for peripheral nerve injury. The films were successfully fabricated and showed improved physical properties such as mechanical strength, swelling ratio, and lengthened degradation period while maintaining the microstructure and chemical composition after GA crosslinking. In the in vitro study of CAM‐film, the dsDNA content met the recommended limit of decellularization and more than 70% of the major ECM components were preserved after decellularization. The adhesion and proliferation of seeded human umbilical vein endothelial cells and fibroblasts were significantly lower in CAM‐film than in control, but similar with Seprafilm. However, the CAM‐film extract did not show cytotoxicity. In the in vivo study, the peri‐neural fibrosis was thicker, adhesion score higher, and peri‐neural collagen fibers more abundant in the control group than in the CAM‐film group. The total number of myelinated axons was significantly higher in the CAM‐film group than in the control group. The inflammatory marker decreased with time in the CAM‐film group compared to that in the control group, whereas the nerve regenerative marker expression was maintained. Moreover, the ankle angles at contracture and toe‐off were higher in the CAM film‐treated rats than in the control rats. GA‐crosslinked CAM films may be used during peripheral nerve surgery to prevent peri‐neural adhesion and enhance nerve functional recovery.</description><subject>Adhesion</subject><subject>Animals</subject><subject>Ankle</subject><subject>Axons</subject><subject>Biocompatibility</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cartilage</subject><subject>Cartilage - chemistry</subject><subject>cartilage acellular matrix</subject><subject>Cell Adhesion</subject><subject>Cell Death</subject><subject>Cell Proliferation</subject><subject>Chemical composition</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Crosslinking</subject><subject>Cytotoxicity</subject><subject>Disease Models, Animal</subject><subject>Endothelial cells</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - chemistry</subject><subject>extra‐cellular matrix</subject><subject>Fibroblasts</subject><subject>Fibrosis</subject><subject>Glutaral - chemistry</subject><subject>Glutaraldehyde</subject><subject>Human Umbilical Vein Endothelial Cells - metabolism</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>Inflammation</subject><subject>Male</subject><subject>Markers</subject><subject>Mechanical properties</subject><subject>Mice</subject><subject>nerve functional recovery</subject><subject>nerve injury</subject><subject>Nerve Regeneration - physiology</subject><subject>Organs</subject><subject>peripheral nerve</subject><subject>Peripheral nerves</subject><subject>Physical properties</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recovery of function</subject><subject>Recovery of Function - drug effects</subject><subject>Regeneration</subject><subject>Regenerative medicine</subject><subject>Sciatic nerve</subject><subject>Sciatic Nerve - immunology</subject><subject>Sciatic Nerve - injuries</subject><subject>Sciatic Nerve - pathology</subject><subject>Sciatic Nerve - physiopathology</subject><subject>Swelling ratio</subject><subject>Swine</subject><subject>Tissue engineering</subject><subject>Toxicity</subject><subject>Umbilical vein</subject><issn>1932-6254</issn><issn>1932-7005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctOGzEYha2qqNC0C16gstQNLAK-JuNlhQJFAiGhsB557N_BwTMD9gyQHY_Aok_YJ8GTpCwqsfLx8efjy0Fon5IjSgg77iDWR5wJ9QntUcXZeEqI_LzVEybFLvqa0jKbciL5F7TLhVSUC7GH_sycA9Ph1uFF6DsddbBwu7Lw9-XVxDal4Js7sNjo2PmgF4C1gRD6oCOudRf9M3Y-1LhtsG46n3dpewvJr-cWNxAfAUdYQFa6G2yfV3DWOBmfHbNlfLPs4wrXrYXwDe04HRJ8344jdHM6m5_8Hl9cnZ2f_LoYG14UalyRwgiuwLhKaVoV1MgpMO7YRBhujCSWqUo5y6bWKpdRWlVWaG4oMVwA5SN0sMm9j-1DD6kra5-G5-kG2j6VTE4LKvMRJKM__0OXbR-bfLtMKcIoI2KSqcMNtf66CK68j77WcVVSUg5VlUNV5VBVZn9sE_uqBvtO_usmA8cb4MkHWH2cVM5n15fryDflVaNk</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Shin, Young Ho</creator><creator>Yun, Hee‐Woong</creator><creator>Park, Suk Young</creator><creator>Choi, Soon Jin</creator><creator>Park, In‐Su</creator><creator>Min, Byoung‐Hyun</creator><creator>Kim, Jae Kwang</creator><general>Hindawi Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6919-5035</orcidid><orcidid>https://orcid.org/0000-0001-5104-4634</orcidid></search><sort><creationdate>202111</creationdate><title>Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model</title><author>Shin, Young Ho ; Yun, Hee‐Woong ; Park, Suk Young ; Choi, Soon Jin ; Park, In‐Su ; Min, Byoung‐Hyun ; Kim, Jae Kwang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3889-b08c439ecfb9a1b81c57e23f264c3cc50d29b9fd27dd9fc431bbd4a3c10c34e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adhesion</topic><topic>Animals</topic><topic>Ankle</topic><topic>Axons</topic><topic>Biocompatibility</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Cartilage</topic><topic>Cartilage - chemistry</topic><topic>cartilage acellular matrix</topic><topic>Cell Adhesion</topic><topic>Cell Death</topic><topic>Cell Proliferation</topic><topic>Chemical composition</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Crosslinking</topic><topic>Cytotoxicity</topic><topic>Disease Models, Animal</topic><topic>Endothelial cells</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - chemistry</topic><topic>extra‐cellular matrix</topic><topic>Fibroblasts</topic><topic>Fibrosis</topic><topic>Glutaral - chemistry</topic><topic>Glutaraldehyde</topic><topic>Human Umbilical Vein Endothelial Cells - metabolism</topic><topic>Humans</topic><topic>In vivo methods and tests</topic><topic>Inflammation</topic><topic>Male</topic><topic>Markers</topic><topic>Mechanical properties</topic><topic>Mice</topic><topic>nerve functional recovery</topic><topic>nerve injury</topic><topic>Nerve Regeneration - physiology</topic><topic>Organs</topic><topic>peripheral nerve</topic><topic>Peripheral nerves</topic><topic>Physical properties</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recovery of function</topic><topic>Recovery of Function - drug effects</topic><topic>Regeneration</topic><topic>Regenerative medicine</topic><topic>Sciatic nerve</topic><topic>Sciatic Nerve - immunology</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Nerve - pathology</topic><topic>Sciatic Nerve - physiopathology</topic><topic>Swelling ratio</topic><topic>Swine</topic><topic>Tissue engineering</topic><topic>Toxicity</topic><topic>Umbilical vein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shin, Young Ho</creatorcontrib><creatorcontrib>Yun, Hee‐Woong</creatorcontrib><creatorcontrib>Park, Suk Young</creatorcontrib><creatorcontrib>Choi, Soon Jin</creatorcontrib><creatorcontrib>Park, In‐Su</creatorcontrib><creatorcontrib>Min, Byoung‐Hyun</creatorcontrib><creatorcontrib>Kim, Jae Kwang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of tissue engineering and regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shin, Young Ho</au><au>Yun, Hee‐Woong</au><au>Park, Suk Young</au><au>Choi, Soon Jin</au><au>Park, In‐Su</au><au>Min, Byoung‐Hyun</au><au>Kim, Jae Kwang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model</atitle><jtitle>Journal of tissue engineering and regenerative medicine</jtitle><addtitle>J Tissue Eng Regen Med</addtitle><date>2021-11</date><risdate>2021</risdate><volume>15</volume><issue>11</issue><spage>1023</spage><epage>1036</epage><pages>1023-1036</pages><issn>1932-6254</issn><eissn>1932-7005</eissn><abstract>Decellularized extra‐cellular matrix (ECM) has been studied as an alternative to anti‐adhesive biomaterials and cartilage acellular matrix (CAM) has been shown to inhibit postoperative adhesion in several organs. This study aimed to evaluate the suitability of glutaraldehyde (GA) crosslinked CAM‐films as anti‐adhesion barriers for peripheral nerve injury. The films were successfully fabricated and showed improved physical properties such as mechanical strength, swelling ratio, and lengthened degradation period while maintaining the microstructure and chemical composition after GA crosslinking. In the in vitro study of CAM‐film, the dsDNA content met the recommended limit of decellularization and more than 70% of the major ECM components were preserved after decellularization. The adhesion and proliferation of seeded human umbilical vein endothelial cells and fibroblasts were significantly lower in CAM‐film than in control, but similar with Seprafilm. However, the CAM‐film extract did not show cytotoxicity. In the in vivo study, the peri‐neural fibrosis was thicker, adhesion score higher, and peri‐neural collagen fibers more abundant in the control group than in the CAM‐film group. The total number of myelinated axons was significantly higher in the CAM‐film group than in the control group. The inflammatory marker decreased with time in the CAM‐film group compared to that in the control group, whereas the nerve regenerative marker expression was maintained. Moreover, the ankle angles at contracture and toe‐off were higher in the CAM film‐treated rats than in the control rats. GA‐crosslinked CAM films may be used during peripheral nerve surgery to prevent peri‐neural adhesion and enhance nerve functional recovery.</abstract><cop>England</cop><pub>Hindawi Limited</pub><pmid>34591344</pmid><doi>10.1002/term.3249</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-6919-5035</orcidid><orcidid>https://orcid.org/0000-0001-5104-4634</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesion Animals Ankle Axons Biocompatibility Biomaterials Biomedical materials Cartilage Cartilage - chemistry cartilage acellular matrix Cell Adhesion Cell Death Cell Proliferation Chemical composition Collagen Collagen - metabolism Cross-Linking Reagents - chemistry Crosslinking Cytotoxicity Disease Models, Animal Endothelial cells Extracellular matrix Extracellular Matrix - chemistry extra‐cellular matrix Fibroblasts Fibrosis Glutaral - chemistry Glutaraldehyde Human Umbilical Vein Endothelial Cells - metabolism Humans In vivo methods and tests Inflammation Male Markers Mechanical properties Mice nerve functional recovery nerve injury Nerve Regeneration - physiology Organs peripheral nerve Peripheral nerves Physical properties Rats Rats, Sprague-Dawley Recovery of function Recovery of Function - drug effects Regeneration Regenerative medicine Sciatic nerve Sciatic Nerve - immunology Sciatic Nerve - injuries Sciatic Nerve - pathology Sciatic Nerve - physiopathology Swelling ratio Swine Tissue engineering Toxicity Umbilical vein |
| title | Effect of glutaraldehyde‐crosslinked cartilage acellular matrix film on anti‐adhesion and nerve regeneration in a rat sciatic nerve injury model |
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