Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides
Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surfac...
Gespeichert in:
| Veröffentlicht in: | ACS chemical biology 2021-10, Vol.16 (10), p.1841-1865 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1865 |
|---|---|
| container_issue | 10 |
| container_start_page | 1841 |
| container_title | ACS chemical biology |
| container_volume | 16 |
| creator | Zheng, Meng Zheng, Maggie Epstein, Samuel Harnagel, Alexa P Kim, Hanee Lupoli, Tania J |
| description | Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surface of the outer membrane. This review highlights chemical biology tools that have been developed in recent years to facilitate the modulation of O-antigen synthesis and composition, as well as related bacterial polysaccharide pathways, and the detection of unique glycan sequences. Advances in the biochemistry and structural biology of O-antigen biosynthetic machinery are also described, which provide guidance for the design of novel chemical and biomolecular probes. Many of the tools noted here have not yet been utilized in biological systems and offer researchers the opportunity to investigate the complex sugar architecture of Gram-negative cells. |
| doi_str_mv | 10.1021/acschembio.1c00341 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2576914942</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2576914942</sourcerecordid><originalsourceid>FETCH-LOGICAL-a342t-6be84170135b22bb5d3ca5d91fe9261b7657230ba740e742b92ba31a5fa4501e3</originalsourceid><addsrcrecordid>eNp9kMlOwzAQhi0EgrK8AAeUI5cUr0l9pBWbxCaxXKOxMymunLrYDVJ5eoJa4MZpPPL3f9L8hBwzOmSUszOwyb5ha1wYMkupkGyLDJhSMh9pUW7_vrneI_spzSiVohjpXbInpCp0qfmA4KQ3OAs-G7vgw3SVPYfgU9aEmN2FuvOwdPNpBvM6e3WpA-8-v_erCG1-j9P-9wOzMdglRtdLnrrYgMXsMfhVAmvfILoa0yHZacAnPNrMA_JyefE8uc5vH65uJue3OQjJl3lhcCRZSZlQhnNjVC0sqFqzBjUvmCkLVXJBDZSSYim50dyAYKAakIoyFAfkdO1dxPDeYVpWrUsWvYc5hi5VXJWFZlJL3qN8jdoYUorYVIvoWoiritHqu97qr95qU28fOtn4O9Ni_Rv56bMHhmugD1ez0MV5f-5_xi_yiYj0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2576914942</pqid></control><display><type>article</type><title>Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides</title><source>ACS Publications</source><source>MEDLINE</source><creator>Zheng, Meng ; Zheng, Maggie ; Epstein, Samuel ; Harnagel, Alexa P ; Kim, Hanee ; Lupoli, Tania J</creator><creatorcontrib>Zheng, Meng ; Zheng, Maggie ; Epstein, Samuel ; Harnagel, Alexa P ; Kim, Hanee ; Lupoli, Tania J</creatorcontrib><description>Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surface of the outer membrane. This review highlights chemical biology tools that have been developed in recent years to facilitate the modulation of O-antigen synthesis and composition, as well as related bacterial polysaccharide pathways, and the detection of unique glycan sequences. Advances in the biochemistry and structural biology of O-antigen biosynthetic machinery are also described, which provide guidance for the design of novel chemical and biomolecular probes. Many of the tools noted here have not yet been utilized in biological systems and offer researchers the opportunity to investigate the complex sugar architecture of Gram-negative cells.</description><identifier>ISSN: 1554-8929</identifier><identifier>EISSN: 1554-8937</identifier><identifier>DOI: 10.1021/acschembio.1c00341</identifier><identifier>PMID: 34569792</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Enzyme Inhibitors - pharmacology ; Glycosyltransferases - antagonists & inhibitors ; Glycosyltransferases - chemistry ; Glycosyltransferases - metabolism ; Gram-Negative Bacteria - chemistry ; Gram-Negative Bacteria - enzymology ; Humans ; Metabolic Engineering ; Molecular Probes - chemistry ; Molecular Probes - pharmacology ; Nucleotidyltransferases - antagonists & inhibitors ; Nucleotidyltransferases - chemistry ; Nucleotidyltransferases - genetics ; Nucleotidyltransferases - metabolism ; O Antigens - chemistry ; O Antigens - metabolism ; Protein Engineering ; Substrate Specificity - genetics</subject><ispartof>ACS chemical biology, 2021-10, Vol.16 (10), p.1841-1865</ispartof><rights>2021 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a342t-6be84170135b22bb5d3ca5d91fe9261b7657230ba740e742b92ba31a5fa4501e3</citedby><cites>FETCH-LOGICAL-a342t-6be84170135b22bb5d3ca5d91fe9261b7657230ba740e742b92ba31a5fa4501e3</cites><orcidid>0000-0003-4425-0039 ; 0000-0002-0989-2565</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acschembio.1c00341$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acschembio.1c00341$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34569792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zheng, Meng</creatorcontrib><creatorcontrib>Zheng, Maggie</creatorcontrib><creatorcontrib>Epstein, Samuel</creatorcontrib><creatorcontrib>Harnagel, Alexa P</creatorcontrib><creatorcontrib>Kim, Hanee</creatorcontrib><creatorcontrib>Lupoli, Tania J</creatorcontrib><title>Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides</title><title>ACS chemical biology</title><addtitle>ACS Chem. Biol</addtitle><description>Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surface of the outer membrane. This review highlights chemical biology tools that have been developed in recent years to facilitate the modulation of O-antigen synthesis and composition, as well as related bacterial polysaccharide pathways, and the detection of unique glycan sequences. Advances in the biochemistry and structural biology of O-antigen biosynthetic machinery are also described, which provide guidance for the design of novel chemical and biomolecular probes. Many of the tools noted here have not yet been utilized in biological systems and offer researchers the opportunity to investigate the complex sugar architecture of Gram-negative cells.</description><subject>Enzyme Inhibitors - pharmacology</subject><subject>Glycosyltransferases - antagonists & inhibitors</subject><subject>Glycosyltransferases - chemistry</subject><subject>Glycosyltransferases - metabolism</subject><subject>Gram-Negative Bacteria - chemistry</subject><subject>Gram-Negative Bacteria - enzymology</subject><subject>Humans</subject><subject>Metabolic Engineering</subject><subject>Molecular Probes - chemistry</subject><subject>Molecular Probes - pharmacology</subject><subject>Nucleotidyltransferases - antagonists & inhibitors</subject><subject>Nucleotidyltransferases - chemistry</subject><subject>Nucleotidyltransferases - genetics</subject><subject>Nucleotidyltransferases - metabolism</subject><subject>O Antigens - chemistry</subject><subject>O Antigens - metabolism</subject><subject>Protein Engineering</subject><subject>Substrate Specificity - genetics</subject><issn>1554-8929</issn><issn>1554-8937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQhi0EgrK8AAeUI5cUr0l9pBWbxCaxXKOxMymunLrYDVJ5eoJa4MZpPPL3f9L8hBwzOmSUszOwyb5ha1wYMkupkGyLDJhSMh9pUW7_vrneI_spzSiVohjpXbInpCp0qfmA4KQ3OAs-G7vgw3SVPYfgU9aEmN2FuvOwdPNpBvM6e3WpA-8-v_erCG1-j9P-9wOzMdglRtdLnrrYgMXsMfhVAmvfILoa0yHZacAnPNrMA_JyefE8uc5vH65uJue3OQjJl3lhcCRZSZlQhnNjVC0sqFqzBjUvmCkLVXJBDZSSYim50dyAYKAakIoyFAfkdO1dxPDeYVpWrUsWvYc5hi5VXJWFZlJL3qN8jdoYUorYVIvoWoiritHqu97qr95qU28fOtn4O9Ni_Rv56bMHhmugD1ez0MV5f-5_xi_yiYj0</recordid><startdate>20211015</startdate><enddate>20211015</enddate><creator>Zheng, Meng</creator><creator>Zheng, Maggie</creator><creator>Epstein, Samuel</creator><creator>Harnagel, Alexa P</creator><creator>Kim, Hanee</creator><creator>Lupoli, Tania J</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4425-0039</orcidid><orcidid>https://orcid.org/0000-0002-0989-2565</orcidid></search><sort><creationdate>20211015</creationdate><title>Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides</title><author>Zheng, Meng ; Zheng, Maggie ; Epstein, Samuel ; Harnagel, Alexa P ; Kim, Hanee ; Lupoli, Tania J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a342t-6be84170135b22bb5d3ca5d91fe9261b7657230ba740e742b92ba31a5fa4501e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Enzyme Inhibitors - pharmacology</topic><topic>Glycosyltransferases - antagonists & inhibitors</topic><topic>Glycosyltransferases - chemistry</topic><topic>Glycosyltransferases - metabolism</topic><topic>Gram-Negative Bacteria - chemistry</topic><topic>Gram-Negative Bacteria - enzymology</topic><topic>Humans</topic><topic>Metabolic Engineering</topic><topic>Molecular Probes - chemistry</topic><topic>Molecular Probes - pharmacology</topic><topic>Nucleotidyltransferases - antagonists & inhibitors</topic><topic>Nucleotidyltransferases - chemistry</topic><topic>Nucleotidyltransferases - genetics</topic><topic>Nucleotidyltransferases - metabolism</topic><topic>O Antigens - chemistry</topic><topic>O Antigens - metabolism</topic><topic>Protein Engineering</topic><topic>Substrate Specificity - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zheng, Meng</creatorcontrib><creatorcontrib>Zheng, Maggie</creatorcontrib><creatorcontrib>Epstein, Samuel</creatorcontrib><creatorcontrib>Harnagel, Alexa P</creatorcontrib><creatorcontrib>Kim, Hanee</creatorcontrib><creatorcontrib>Lupoli, Tania J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Meng</au><au>Zheng, Maggie</au><au>Epstein, Samuel</au><au>Harnagel, Alexa P</au><au>Kim, Hanee</au><au>Lupoli, Tania J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides</atitle><jtitle>ACS chemical biology</jtitle><addtitle>ACS Chem. Biol</addtitle><date>2021-10-15</date><risdate>2021</risdate><volume>16</volume><issue>10</issue><spage>1841</spage><epage>1865</epage><pages>1841-1865</pages><issn>1554-8929</issn><eissn>1554-8937</eissn><abstract>Bacterial cells present a wide diversity of saccharides that decorate the cell surface and help mediate interactions with the environment. Many Gram-negative cells express O-antigens, which are long sugar polymers that makeup the distal portion of lipopolysaccharide (LPS) that constitutes the surface of the outer membrane. This review highlights chemical biology tools that have been developed in recent years to facilitate the modulation of O-antigen synthesis and composition, as well as related bacterial polysaccharide pathways, and the detection of unique glycan sequences. Advances in the biochemistry and structural biology of O-antigen biosynthetic machinery are also described, which provide guidance for the design of novel chemical and biomolecular probes. Many of the tools noted here have not yet been utilized in biological systems and offer researchers the opportunity to investigate the complex sugar architecture of Gram-negative cells.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>34569792</pmid><doi>10.1021/acschembio.1c00341</doi><tpages>25</tpages><orcidid>https://orcid.org/0000-0003-4425-0039</orcidid><orcidid>https://orcid.org/0000-0002-0989-2565</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1554-8929 |
| ispartof | ACS chemical biology, 2021-10, Vol.16 (10), p.1841-1865 |
| issn | 1554-8929 1554-8937 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2576914942 |
| source | ACS Publications; MEDLINE |
| subjects | Enzyme Inhibitors - pharmacology Glycosyltransferases - antagonists & inhibitors Glycosyltransferases - chemistry Glycosyltransferases - metabolism Gram-Negative Bacteria - chemistry Gram-Negative Bacteria - enzymology Humans Metabolic Engineering Molecular Probes - chemistry Molecular Probes - pharmacology Nucleotidyltransferases - antagonists & inhibitors Nucleotidyltransferases - chemistry Nucleotidyltransferases - genetics Nucleotidyltransferases - metabolism O Antigens - chemistry O Antigens - metabolism Protein Engineering Substrate Specificity - genetics |
| title | Chemical Biology Tools for Modulating and Visualizing Gram-Negative Bacterial Surface Polysaccharides |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T15%3A24%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chemical%20Biology%20Tools%20for%20Modulating%20and%20Visualizing%20Gram-Negative%20Bacterial%20Surface%20Polysaccharides&rft.jtitle=ACS%20chemical%20biology&rft.au=Zheng,%20Meng&rft.date=2021-10-15&rft.volume=16&rft.issue=10&rft.spage=1841&rft.epage=1865&rft.pages=1841-1865&rft.issn=1554-8929&rft.eissn=1554-8937&rft_id=info:doi/10.1021/acschembio.1c00341&rft_dat=%3Cproquest_cross%3E2576914942%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2576914942&rft_id=info:pmid/34569792&rfr_iscdi=true |