Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey
The emerging situation of Salmonella enterica subsp. enterica serovar Infantis ( S . Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as Salmonella genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) a...
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| Veröffentlicht in: | International microbiology 2022-05, Vol.25 (2), p.259-265 |
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| creator | Namli, Sahin Soyer, Yesim |
| description | The emerging situation of
Salmonella enterica
subsp.
enterica
serovar Infantis (
S
. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as
Salmonella
genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was conducted with real-time PCR for 70
S
. Infantis isolates from poultry products. SGI1 or its variants were not detected in any of the isolates. Sixty-eight of 70 isolates were detected to carry one C1 integron of size 1.0 kb. These integrons were detected to carry
ant(3″)-Ia
gene cassette explaining the streptomycin/spectinomycin resistance. Sequence analysis of gene cassettes belongs to four representing isolates which showed that, although their difference in isolation date and place, genetically, they are 99.9% similar. Virulence gene screening was introduced as genotypic virulence profiles. The most dominant profile for
S
. Infantis isolates, among twelve genes, was
gatC
-
tcfA
, which are known to be related to colonization at specific hosts. This study revealed the high percentage of C1 integron possession in
S
. Infantis isolates from poultry products in Turkey. It also showed the potential of
S
. Infantis strains to be resistant to more antimicrobial drugs. Moreover, a dominant profile of virulence genes that are uncommon for non-typhoidal
Salmonella
(NTS) serovars was detected, which might explain the enhanced growth at specified hosts. |
| doi_str_mv | 10.1007/s10123-021-00212-x |
| format | Article |
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Salmonella enterica
subsp.
enterica
serovar Infantis (
S
. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as
Salmonella
genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was conducted with real-time PCR for 70
S
. Infantis isolates from poultry products. SGI1 or its variants were not detected in any of the isolates. Sixty-eight of 70 isolates were detected to carry one C1 integron of size 1.0 kb. These integrons were detected to carry
ant(3″)-Ia
gene cassette explaining the streptomycin/spectinomycin resistance. Sequence analysis of gene cassettes belongs to four representing isolates which showed that, although their difference in isolation date and place, genetically, they are 99.9% similar. Virulence gene screening was introduced as genotypic virulence profiles. The most dominant profile for
S
. Infantis isolates, among twelve genes, was
gatC
-
tcfA
, which are known to be related to colonization at specific hosts. This study revealed the high percentage of C1 integron possession in
S
. Infantis isolates from poultry products in Turkey. It also showed the potential of
S
. Infantis strains to be resistant to more antimicrobial drugs. Moreover, a dominant profile of virulence genes that are uncommon for non-typhoidal
Salmonella
(NTS) serovars was detected, which might explain the enhanced growth at specified hosts.</description><identifier>ISSN: 1618-1905</identifier><identifier>ISSN: 1139-6709</identifier><identifier>EISSN: 1618-1905</identifier><identifier>DOI: 10.1007/s10123-021-00212-x</identifier><identifier>PMID: 34559352</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Antiinfectives and antibacterials ; Antimicrobial agents ; Applied Microbiology ; Biomedical and Life Sciences ; Cassettes ; Colonization ; Eukaryotic Microbiology ; Genes ; Genetic screening ; Genomic islands ; Human performance ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Multidrug resistance ; Original Article ; Poultry ; Salmonella ; Screening ; Sequence analysis ; Spectinomycin ; Streptomycin ; Virulence</subject><ispartof>International microbiology, 2022-05, Vol.25 (2), p.259-265</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Switzerland AG 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.</rights><rights>Copyright Spanish Society for Microbiology May 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-cefe010541dad460a4476847cac117fa79f7c37921fa38df760bb18b5d4948003</citedby><cites>FETCH-LOGICAL-c375t-cefe010541dad460a4476847cac117fa79f7c37921fa38df760bb18b5d4948003</cites><orcidid>0000-0003-0187-9232 ; 0000-0001-9687-9715</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10123-021-00212-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10123-021-00212-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34559352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Namli, Sahin</creatorcontrib><creatorcontrib>Soyer, Yesim</creatorcontrib><title>Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey</title><title>International microbiology</title><addtitle>Int Microbiol</addtitle><addtitle>Int Microbiol</addtitle><description>The emerging situation of
Salmonella enterica
subsp.
enterica
serovar Infantis (
S
. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as
Salmonella
genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was conducted with real-time PCR for 70
S
. Infantis isolates from poultry products. SGI1 or its variants were not detected in any of the isolates. Sixty-eight of 70 isolates were detected to carry one C1 integron of size 1.0 kb. These integrons were detected to carry
ant(3″)-Ia
gene cassette explaining the streptomycin/spectinomycin resistance. Sequence analysis of gene cassettes belongs to four representing isolates which showed that, although their difference in isolation date and place, genetically, they are 99.9% similar. Virulence gene screening was introduced as genotypic virulence profiles. The most dominant profile for
S
. Infantis isolates, among twelve genes, was
gatC
-
tcfA
, which are known to be related to colonization at specific hosts. This study revealed the high percentage of C1 integron possession in
S
. Infantis isolates from poultry products in Turkey. It also showed the potential of
S
. Infantis strains to be resistant to more antimicrobial drugs. Moreover, a dominant profile of virulence genes that are uncommon for non-typhoidal
Salmonella
(NTS) serovars was detected, which might explain the enhanced growth at specified hosts.</description><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Applied Microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Cassettes</subject><subject>Colonization</subject><subject>Eukaryotic Microbiology</subject><subject>Genes</subject><subject>Genetic screening</subject><subject>Genomic islands</subject><subject>Human performance</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Multidrug resistance</subject><subject>Original Article</subject><subject>Poultry</subject><subject>Salmonella</subject><subject>Screening</subject><subject>Sequence analysis</subject><subject>Spectinomycin</subject><subject>Streptomycin</subject><subject>Virulence</subject><issn>1618-1905</issn><issn>1139-6709</issn><issn>1618-1905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1TAQhS0Eog_4AyyQJTZsAjN-JktUFbhSJRaUteWbjC8piV3s5Kr997i9LSAWbGzL882ZYx_GXiG8QwD7viCgkA0IbKAuorl5wo7RYNtgB_rpX-cjdlLKFQBa08JzdiSV1p3U4phNm7insow7v4wp8hR4P_lSOPIxLrTLKRbu48D3Y14nij3xHUUqtcqX78RpplwvFv7VT3OKNE2eF8pp7zPfxODjMt6zl2v-Qbcv2LPgp0IvH_ZT9u3j-eXZ5-biy6fN2YeLppdWL01PgQBBKxz8oAx4papvZXvfI9rgbRdsJTuBwct2CNbAdovtVg-qUy2APGVvD7rXOf1c6_PcPJb-zlyktBYntDVGW2nair75B71Ka47VnRPGClBGdlgpcaD6nErJFNx1Hmefbx2Cu8vCHbJwNQZ3n4W7qU2vH6TX7UzD75bHz6-APAClluKO8p_Z_5H9BQCnlQQ</recordid><startdate>20220501</startdate><enddate>20220501</enddate><creator>Namli, Sahin</creator><creator>Soyer, Yesim</creator><general>Springer International Publishing</general><general>Spanish Society for Microbiology</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0187-9232</orcidid><orcidid>https://orcid.org/0000-0001-9687-9715</orcidid></search><sort><creationdate>20220501</creationdate><title>Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey</title><author>Namli, Sahin ; Soyer, Yesim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-cefe010541dad460a4476847cac117fa79f7c37921fa38df760bb18b5d4948003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Applied Microbiology</topic><topic>Biomedical and Life Sciences</topic><topic>Cassettes</topic><topic>Colonization</topic><topic>Eukaryotic Microbiology</topic><topic>Genes</topic><topic>Genetic screening</topic><topic>Genomic islands</topic><topic>Human performance</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Multidrug resistance</topic><topic>Original Article</topic><topic>Poultry</topic><topic>Salmonella</topic><topic>Screening</topic><topic>Sequence analysis</topic><topic>Spectinomycin</topic><topic>Streptomycin</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Namli, Sahin</creatorcontrib><creatorcontrib>Soyer, Yesim</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>International microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Namli, Sahin</au><au>Soyer, Yesim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey</atitle><jtitle>International microbiology</jtitle><stitle>Int Microbiol</stitle><addtitle>Int Microbiol</addtitle><date>2022-05-01</date><risdate>2022</risdate><volume>25</volume><issue>2</issue><spage>259</spage><epage>265</epage><pages>259-265</pages><issn>1618-1905</issn><issn>1139-6709</issn><eissn>1618-1905</eissn><abstract>The emerging situation of
Salmonella enterica
subsp.
enterica
serovar Infantis (
S
. Infantis) in Turkey was investigated in terms of virulence genes and mobile genetic elements such as
Salmonella
genomic island 1 (SGI1) and class 1 (C1) integron to see whether increased multidrug resistance (MDR) and ability to cause human cases is a consequence of their possession. Screening of SGI1 (and its variants) and C1 integrons was done with conventional PCR, while screening of gene cassettes and virulence genes was conducted with real-time PCR for 70
S
. Infantis isolates from poultry products. SGI1 or its variants were not detected in any of the isolates. Sixty-eight of 70 isolates were detected to carry one C1 integron of size 1.0 kb. These integrons were detected to carry
ant(3″)-Ia
gene cassette explaining the streptomycin/spectinomycin resistance. Sequence analysis of gene cassettes belongs to four representing isolates which showed that, although their difference in isolation date and place, genetically, they are 99.9% similar. Virulence gene screening was introduced as genotypic virulence profiles. The most dominant profile for
S
. Infantis isolates, among twelve genes, was
gatC
-
tcfA
, which are known to be related to colonization at specific hosts. This study revealed the high percentage of C1 integron possession in
S
. Infantis isolates from poultry products in Turkey. It also showed the potential of
S
. Infantis strains to be resistant to more antimicrobial drugs. Moreover, a dominant profile of virulence genes that are uncommon for non-typhoidal
Salmonella
(NTS) serovars was detected, which might explain the enhanced growth at specified hosts.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>34559352</pmid><doi>10.1007/s10123-021-00212-x</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0187-9232</orcidid><orcidid>https://orcid.org/0000-0001-9687-9715</orcidid></addata></record> |
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| subjects | Antiinfectives and antibacterials Antimicrobial agents Applied Microbiology Biomedical and Life Sciences Cassettes Colonization Eukaryotic Microbiology Genes Genetic screening Genomic islands Human performance Life Sciences Medical Microbiology Microbial Ecology Microbiology Multidrug resistance Original Article Poultry Salmonella Screening Sequence analysis Spectinomycin Streptomycin Virulence |
| title | Investigation of class 1 integrons and virulence genes in the emergent Salmonella serovar Infantis in Turkey |
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