Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis
Background Stem cell therapy has been proven to rescue intestinal injury and stimulate intestinal regeneration in necrotizing enterocolitis (NEC). Specifically, stem cells derived from amniotic fluid (AFSCs) and mesenchymal stem cells (MSCs) derived from bone marrow have shown promising results in t...
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| Veröffentlicht in: | Pediatric research 2022-01, Vol.91 (1), p.101-106 |
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| creator | Li, Bo Lee, Carol Cadete, Marissa O’Connell, Joshua S. Alganabi, Mashriq Lee, Dorothy Ganji, Niloofar Miyake, Hiromu Botts, Steven R. Johnson-Henry, Kathene C. Maattanen, Pekka Sherman, Philip M. Pierro, Agostino |
| description | Background
Stem cell therapy has been proven to rescue intestinal injury and stimulate intestinal regeneration in necrotizing enterocolitis (NEC). Specifically, stem cells derived from amniotic fluid (AFSCs) and mesenchymal stem cells (MSCs) derived from bone marrow have shown promising results in the treatment of experimental NEC. This study aims to examine the effects of AFSCs and MSCs on the prevention of intestinal injury during experimental NEC.
Methods
Supernatants from AFSC and MSC cultures were collected to perform proteomic analysis. Prior to NEC induction, mice received intraperitoneal injections of phosphate-buffered saline (PBS), 2 × 10
6
AFSCs, or 2 × 10
6
MSCs.
Results
We found that AFSCs grew faster than MSCs. Proteomic analysis indicated that AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation. Administering AFSCs before NEC induction decreased NEC severity and mucosal inflammation. Intestinal proliferation and endogenous stem cell activation were increased after AFSC administration. However, administering MSCs before NEC induction had no beneficial effects.
Conclusions
This study demonstrated that AFSCs and MSCs have different protein release profiles. AFSCs can potentially be used as a preventative strategy for neonates at risk of NEC, while MSCs cannot be used.
Impact
AFSCs and MSCs have distinct protein secretory profiles, and AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation.
AFSCs are unique in transiently enhancing healthy intestinal epithelial cell growth, which offers protection against the development of experimental NEC.
The prevention of NEC via the administration of AFSCs should be evaluated in infants at great risk of developing NEC or in infants with early signs of NEC. |
| doi_str_mv | 10.1038/s41390-021-01657-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2576655058</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2576655058</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-dfd60f6e6c141e011ea7400f47bc3ab1a042730af4d237616002482e3ea369773</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxS0EotvCF-CALHHhEpjx3-RYVUArVeICZ8ubTIpXibO1nUrtp8dlWyr10NNInt979vNj7APCFwTZfs0KZQcNCGwAjbaNecU2qGU9Usq-ZhsAiY3suvaIHee8A0ClW_WWHUmlDWoNGzaczjEsJfR8nNYw8Fxo5j1NE_fDHGLIJfkSlsh7H_k-0Q3Fwmkfyh-agp94iLs13fIxLTOP1KdqdRfiFa8YpaVfplBCfsfejH7K9P5hnrDf37_9OjtvLn_-uDg7vWx6aXVphnEwMBoyPSokQCRvFcCo7LaXfoselLAS_KgGIa1BAyBUK0iSl6azVp6wzwfffVquV8rFzSHfh_GRljU7oa0xNbZuK_rpGbpb1hTr65wwAqGzyupKiQNVg-WcaHT7FGafbh2Cu-_AHTpwtQP3rwNnqujjg_W6nWn4L3n89ArIA5DrKl5Rerr7Bdu_jlGSWw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2621097475</pqid></control><display><type>article</type><title>Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Li, Bo ; Lee, Carol ; Cadete, Marissa ; O’Connell, Joshua S. ; Alganabi, Mashriq ; Lee, Dorothy ; Ganji, Niloofar ; Miyake, Hiromu ; Botts, Steven R. ; Johnson-Henry, Kathene C. ; Maattanen, Pekka ; Sherman, Philip M. ; Pierro, Agostino</creator><creatorcontrib>Li, Bo ; Lee, Carol ; Cadete, Marissa ; O’Connell, Joshua S. ; Alganabi, Mashriq ; Lee, Dorothy ; Ganji, Niloofar ; Miyake, Hiromu ; Botts, Steven R. ; Johnson-Henry, Kathene C. ; Maattanen, Pekka ; Sherman, Philip M. ; Pierro, Agostino</creatorcontrib><description>Background
Stem cell therapy has been proven to rescue intestinal injury and stimulate intestinal regeneration in necrotizing enterocolitis (NEC). Specifically, stem cells derived from amniotic fluid (AFSCs) and mesenchymal stem cells (MSCs) derived from bone marrow have shown promising results in the treatment of experimental NEC. This study aims to examine the effects of AFSCs and MSCs on the prevention of intestinal injury during experimental NEC.
Methods
Supernatants from AFSC and MSC cultures were collected to perform proteomic analysis. Prior to NEC induction, mice received intraperitoneal injections of phosphate-buffered saline (PBS), 2 × 10
6
AFSCs, or 2 × 10
6
MSCs.
Results
We found that AFSCs grew faster than MSCs. Proteomic analysis indicated that AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation. Administering AFSCs before NEC induction decreased NEC severity and mucosal inflammation. Intestinal proliferation and endogenous stem cell activation were increased after AFSC administration. However, administering MSCs before NEC induction had no beneficial effects.
Conclusions
This study demonstrated that AFSCs and MSCs have different protein release profiles. AFSCs can potentially be used as a preventative strategy for neonates at risk of NEC, while MSCs cannot be used.
Impact
AFSCs and MSCs have distinct protein secretory profiles, and AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation.
AFSCs are unique in transiently enhancing healthy intestinal epithelial cell growth, which offers protection against the development of experimental NEC.
The prevention of NEC via the administration of AFSCs should be evaluated in infants at great risk of developing NEC or in infants with early signs of NEC.</description><identifier>ISSN: 0031-3998</identifier><identifier>EISSN: 1530-0447</identifier><identifier>DOI: 10.1038/s41390-021-01657-6</identifier><identifier>PMID: 34561550</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Amniotic fluid ; Amniotic Fluid - cytology ; Animals ; Basic Science Article ; Enterocolitis, Necrotizing ; Gastrointestinal diseases ; Humans ; Infant, Newborn ; Medicine ; Medicine & Public Health ; Mice ; Necrosis ; Pediatric Surgery ; Pediatrics ; Stem Cell Transplantation ; Stem cells</subject><ispartof>Pediatric research, 2022-01, Vol.91 (1), p.101-106</ispartof><rights>The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc 2021</rights><rights>2021. The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc.</rights><rights>The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-dfd60f6e6c141e011ea7400f47bc3ab1a042730af4d237616002482e3ea369773</citedby><cites>FETCH-LOGICAL-c375t-dfd60f6e6c141e011ea7400f47bc3ab1a042730af4d237616002482e3ea369773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34561550$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Lee, Carol</creatorcontrib><creatorcontrib>Cadete, Marissa</creatorcontrib><creatorcontrib>O’Connell, Joshua S.</creatorcontrib><creatorcontrib>Alganabi, Mashriq</creatorcontrib><creatorcontrib>Lee, Dorothy</creatorcontrib><creatorcontrib>Ganji, Niloofar</creatorcontrib><creatorcontrib>Miyake, Hiromu</creatorcontrib><creatorcontrib>Botts, Steven R.</creatorcontrib><creatorcontrib>Johnson-Henry, Kathene C.</creatorcontrib><creatorcontrib>Maattanen, Pekka</creatorcontrib><creatorcontrib>Sherman, Philip M.</creatorcontrib><creatorcontrib>Pierro, Agostino</creatorcontrib><title>Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis</title><title>Pediatric research</title><addtitle>Pediatr Res</addtitle><addtitle>Pediatr Res</addtitle><description>Background
Stem cell therapy has been proven to rescue intestinal injury and stimulate intestinal regeneration in necrotizing enterocolitis (NEC). Specifically, stem cells derived from amniotic fluid (AFSCs) and mesenchymal stem cells (MSCs) derived from bone marrow have shown promising results in the treatment of experimental NEC. This study aims to examine the effects of AFSCs and MSCs on the prevention of intestinal injury during experimental NEC.
Methods
Supernatants from AFSC and MSC cultures were collected to perform proteomic analysis. Prior to NEC induction, mice received intraperitoneal injections of phosphate-buffered saline (PBS), 2 × 10
6
AFSCs, or 2 × 10
6
MSCs.
Results
We found that AFSCs grew faster than MSCs. Proteomic analysis indicated that AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation. Administering AFSCs before NEC induction decreased NEC severity and mucosal inflammation. Intestinal proliferation and endogenous stem cell activation were increased after AFSC administration. However, administering MSCs before NEC induction had no beneficial effects.
Conclusions
This study demonstrated that AFSCs and MSCs have different protein release profiles. AFSCs can potentially be used as a preventative strategy for neonates at risk of NEC, while MSCs cannot be used.
Impact
AFSCs and MSCs have distinct protein secretory profiles, and AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation.
AFSCs are unique in transiently enhancing healthy intestinal epithelial cell growth, which offers protection against the development of experimental NEC.
The prevention of NEC via the administration of AFSCs should be evaluated in infants at great risk of developing NEC or in infants with early signs of NEC.</description><subject>Amniotic fluid</subject><subject>Amniotic Fluid - cytology</subject><subject>Animals</subject><subject>Basic Science Article</subject><subject>Enterocolitis, Necrotizing</subject><subject>Gastrointestinal diseases</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Necrosis</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><issn>0031-3998</issn><issn>1530-0447</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kU9v1DAQxS0EotvCF-CALHHhEpjx3-RYVUArVeICZ8ubTIpXibO1nUrtp8dlWyr10NNInt979vNj7APCFwTZfs0KZQcNCGwAjbaNecU2qGU9Usq-ZhsAiY3suvaIHee8A0ClW_WWHUmlDWoNGzaczjEsJfR8nNYw8Fxo5j1NE_fDHGLIJfkSlsh7H_k-0Q3Fwmkfyh-agp94iLs13fIxLTOP1KdqdRfiFa8YpaVfplBCfsfejH7K9P5hnrDf37_9OjtvLn_-uDg7vWx6aXVphnEwMBoyPSokQCRvFcCo7LaXfoselLAS_KgGIa1BAyBUK0iSl6azVp6wzwfffVquV8rFzSHfh_GRljU7oa0xNbZuK_rpGbpb1hTr65wwAqGzyupKiQNVg-WcaHT7FGafbh2Cu-_AHTpwtQP3rwNnqujjg_W6nWn4L3n89ArIA5DrKl5Rerr7Bdu_jlGSWw</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Li, Bo</creator><creator>Lee, Carol</creator><creator>Cadete, Marissa</creator><creator>O’Connell, Joshua S.</creator><creator>Alganabi, Mashriq</creator><creator>Lee, Dorothy</creator><creator>Ganji, Niloofar</creator><creator>Miyake, Hiromu</creator><creator>Botts, Steven R.</creator><creator>Johnson-Henry, Kathene C.</creator><creator>Maattanen, Pekka</creator><creator>Sherman, Philip M.</creator><creator>Pierro, Agostino</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20220101</creationdate><title>Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis</title><author>Li, Bo ; Lee, Carol ; Cadete, Marissa ; O’Connell, Joshua S. ; Alganabi, Mashriq ; Lee, Dorothy ; Ganji, Niloofar ; Miyake, Hiromu ; Botts, Steven R. ; Johnson-Henry, Kathene C. ; Maattanen, Pekka ; Sherman, Philip M. ; Pierro, Agostino</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-dfd60f6e6c141e011ea7400f47bc3ab1a042730af4d237616002482e3ea369773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amniotic fluid</topic><topic>Amniotic Fluid - cytology</topic><topic>Animals</topic><topic>Basic Science Article</topic><topic>Enterocolitis, Necrotizing</topic><topic>Gastrointestinal diseases</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Necrosis</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Bo</creatorcontrib><creatorcontrib>Lee, Carol</creatorcontrib><creatorcontrib>Cadete, Marissa</creatorcontrib><creatorcontrib>O’Connell, Joshua S.</creatorcontrib><creatorcontrib>Alganabi, Mashriq</creatorcontrib><creatorcontrib>Lee, Dorothy</creatorcontrib><creatorcontrib>Ganji, Niloofar</creatorcontrib><creatorcontrib>Miyake, Hiromu</creatorcontrib><creatorcontrib>Botts, Steven R.</creatorcontrib><creatorcontrib>Johnson-Henry, Kathene C.</creatorcontrib><creatorcontrib>Maattanen, Pekka</creatorcontrib><creatorcontrib>Sherman, Philip M.</creatorcontrib><creatorcontrib>Pierro, Agostino</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Bo</au><au>Lee, Carol</au><au>Cadete, Marissa</au><au>O’Connell, Joshua S.</au><au>Alganabi, Mashriq</au><au>Lee, Dorothy</au><au>Ganji, Niloofar</au><au>Miyake, Hiromu</au><au>Botts, Steven R.</au><au>Johnson-Henry, Kathene C.</au><au>Maattanen, Pekka</au><au>Sherman, Philip M.</au><au>Pierro, Agostino</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis</atitle><jtitle>Pediatric research</jtitle><stitle>Pediatr Res</stitle><addtitle>Pediatr Res</addtitle><date>2022-01-01</date><risdate>2022</risdate><volume>91</volume><issue>1</issue><spage>101</spage><epage>106</epage><pages>101-106</pages><issn>0031-3998</issn><eissn>1530-0447</eissn><abstract>Background
Stem cell therapy has been proven to rescue intestinal injury and stimulate intestinal regeneration in necrotizing enterocolitis (NEC). Specifically, stem cells derived from amniotic fluid (AFSCs) and mesenchymal stem cells (MSCs) derived from bone marrow have shown promising results in the treatment of experimental NEC. This study aims to examine the effects of AFSCs and MSCs on the prevention of intestinal injury during experimental NEC.
Methods
Supernatants from AFSC and MSC cultures were collected to perform proteomic analysis. Prior to NEC induction, mice received intraperitoneal injections of phosphate-buffered saline (PBS), 2 × 10
6
AFSCs, or 2 × 10
6
MSCs.
Results
We found that AFSCs grew faster than MSCs. Proteomic analysis indicated that AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation. Administering AFSCs before NEC induction decreased NEC severity and mucosal inflammation. Intestinal proliferation and endogenous stem cell activation were increased after AFSC administration. However, administering MSCs before NEC induction had no beneficial effects.
Conclusions
This study demonstrated that AFSCs and MSCs have different protein release profiles. AFSCs can potentially be used as a preventative strategy for neonates at risk of NEC, while MSCs cannot be used.
Impact
AFSCs and MSCs have distinct protein secretory profiles, and AFSCs are primarily involved in cell development and growth, while MSCs are involved in immune regulation.
AFSCs are unique in transiently enhancing healthy intestinal epithelial cell growth, which offers protection against the development of experimental NEC.
The prevention of NEC via the administration of AFSCs should be evaluated in infants at great risk of developing NEC or in infants with early signs of NEC.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>34561550</pmid><doi>10.1038/s41390-021-01657-6</doi><tpages>6</tpages></addata></record> |
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| ispartof | Pediatric research, 2022-01, Vol.91 (1), p.101-106 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amniotic fluid Amniotic Fluid - cytology Animals Basic Science Article Enterocolitis, Necrotizing Gastrointestinal diseases Humans Infant, Newborn Medicine Medicine & Public Health Mice Necrosis Pediatric Surgery Pediatrics Stem Cell Transplantation Stem cells |
| title | Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis |
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