Harnessing snakehead rhabdovirus (SHRV) for gene editing by installment of CRISPR/Cas9 in viral genome

●Recombinant SHRVs harboring CRISPR/Cas9 system in genome were rescued.●rSHRV having sgRNA for BHK-21 cell's IRF9 gene led to indels of the target gene.●rSHRVs could be removed from BHK-21 cells by temperature up-shift to 37 °C.●This is the first report on the use of piscine RNA virus for gene...

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Veröffentlicht in:	Virus research 2021-11, Vol.305, p.198578-198578, Article 198578
Hauptverfasser:	Kwak, Jun Soung, Kim, Min Sun, Kim, Ki Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BHK-21 cells Cricetinae CRISPR-Cas Systems Delivery of CRISPR/Cas9 Gene Editing Genome, Viral IRF9 gene knockout Mammals Novirhabdovirus - genetics Recombinant snakehead rhabdovirus (SHRV)
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