Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors
[Display omitted] •Anisakis antigens significantly interact with TLR2, TLR4 and TLR9.•BALB/c mice presented acute-inflammatory responses.•C57BL/6J mice developed discreet and resistant responses. The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its...
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| Veröffentlicht in: | International immunopharmacology 2021-11, Vol.100, p.108120-108120, Article 108120 |
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| creator | Zamora, Vega Carlos Andreu-Ballester, Juan Rodero, Marta Cuéllar, Carmen |
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•Anisakis antigens significantly interact with TLR2, TLR4 and TLR9.•BALB/c mice presented acute-inflammatory responses.•C57BL/6J mice developed discreet and resistant responses.
The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).
In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2–9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA.
Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/Treg response, but also it states A. simplex larval antigens present specific mechanisms to modify the innate response of the host. As allergies share common pathways with the immune response against this particular helminth, our results provide a better understanding into the specific mechanisms of A. simplex allergy related diseases. |
| doi_str_mv | 10.1016/j.intimp.2021.108120 |
| format | Article |
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•Anisakis antigens significantly interact with TLR2, TLR4 and TLR9.•BALB/c mice presented acute-inflammatory responses.•C57BL/6J mice developed discreet and resistant responses.
The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).
In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2–9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA.
Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/Treg response, but also it states A. simplex larval antigens present specific mechanisms to modify the innate response of the host. As allergies share common pathways with the immune response against this particular helminth, our results provide a better understanding into the specific mechanisms of A. simplex allergy related diseases.</description><identifier>ISSN: 1567-5769</identifier><identifier>EISSN: 1878-1705</identifier><identifier>DOI: 10.1016/j.intimp.2021.108120</identifier><identifier>PMID: 34537480</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Agonists ; Allergies ; Animals ; Anisakis - immunology ; Anisakis simplex ; Antigens ; Antigens - immunology ; Bone marrow ; CD11c antigen ; CD80 antigen ; Cytokine ; Cytokines ; Dendritic Cell ; Dendritic cells ; Enzyme-linked immunosorbent assay ; Female ; Flow cytometry ; H-2 Antigens ; Immune response ; Immune system ; Immunomodulation ; Immunoregulation ; Inflammation ; Inflammatory response ; Innate Immunity ; Interleukin 10 ; Interleukin 12 ; Interleukin-10 - metabolism ; Interleukin-12 - metabolism ; Intracellular ; Larva - immunology ; Lymphocytes T ; Major histocompatibility complex ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; MyD88 protein ; Proteins ; Receptors ; Surface markers ; TLR2 protein ; TLR3 protein ; TLR4 protein ; TLR5 protein ; TLR7 protein ; Toll-Like Receptor 2 ; Toll-like receptors ; Toll-Like Receptors - agonists ; Toll-Like Receptors - immunology</subject><ispartof>International immunopharmacology, 2021-11, Vol.100, p.108120-108120, Article 108120</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright © 2021 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Nov 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-9eee3a18090c3fbec7936772df2f56edac28325d3a0ccc6801cd00fded1ca8cf3</citedby><cites>FETCH-LOGICAL-c390t-9eee3a18090c3fbec7936772df2f56edac28325d3a0ccc6801cd00fded1ca8cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567576921007566$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34537480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zamora, Vega</creatorcontrib><creatorcontrib>Carlos Andreu-Ballester, Juan</creatorcontrib><creatorcontrib>Rodero, Marta</creatorcontrib><creatorcontrib>Cuéllar, Carmen</creatorcontrib><title>Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors</title><title>International immunopharmacology</title><addtitle>Int Immunopharmacol</addtitle><description>[Display omitted]
•Anisakis antigens significantly interact with TLR2, TLR4 and TLR9.•BALB/c mice presented acute-inflammatory responses.•C57BL/6J mice developed discreet and resistant responses.
The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).
In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2–9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA.
Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/Treg response, but also it states A. simplex larval antigens present specific mechanisms to modify the innate response of the host. As allergies share common pathways with the immune response against this particular helminth, our results provide a better understanding into the specific mechanisms of A. simplex allergy related diseases.</description><subject>Agonists</subject><subject>Allergies</subject><subject>Animals</subject><subject>Anisakis - immunology</subject><subject>Anisakis simplex</subject><subject>Antigens</subject><subject>Antigens - immunology</subject><subject>Bone marrow</subject><subject>CD11c antigen</subject><subject>CD80 antigen</subject><subject>Cytokine</subject><subject>Cytokines</subject><subject>Dendritic Cell</subject><subject>Dendritic cells</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>H-2 Antigens</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunomodulation</subject><subject>Immunoregulation</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Innate Immunity</subject><subject>Interleukin 10</subject><subject>Interleukin 12</subject><subject>Interleukin-10 - metabolism</subject><subject>Interleukin-12 - metabolism</subject><subject>Intracellular</subject><subject>Larva - immunology</subject><subject>Lymphocytes T</subject><subject>Major histocompatibility complex</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>MyD88 protein</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Surface markers</subject><subject>TLR2 protein</subject><subject>TLR3 protein</subject><subject>TLR4 protein</subject><subject>TLR5 protein</subject><subject>TLR7 protein</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-like receptors</subject><subject>Toll-Like Receptors - agonists</subject><subject>Toll-Like Receptors - immunology</subject><issn>1567-5769</issn><issn>1878-1705</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9vFSEUxYnR2Fr9BsaQuHEzzwsMA-PCpGn806SJialrQuGivDLDE2Ze7LeXZqoLF66Ak989l5xDyEsGOwZseLvfxXmJ02HHgbMmacbhETllWumOKZCP210OqpNqGE_Is1r3AE3v2VNyInopVK_hlITzOVZ7GyutzSvhr3f0cprWOU_Zr8kuudxRDAHdUmkONNlytInatvk7zk2a6fIDqXVLPNoltmeDrnNKNMVbpF_R4aF51OfkSbCp4ouH84x8-_jh-uJzd_Xl0-XF-VXnxAhLNyKisEzDCE6EG3RqFINS3Ace5IDeOq4Fl15YcM4NGpjzAMGjZ85qF8QZebP5Hkr-uWJdzBSrw5TsjHmthkvVq17osW_o63_QfV7L3H5n-ABSgGyZNqrfKFdyrQWDOZQ42XJnGJj7HszebD2Y-x7M1kMbe_Vgvt5M6P8O_Qm-Ae83AFsax4jFVBdxduhjaWEbn-P_N_wGTHicpQ</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Zamora, Vega</creator><creator>Carlos Andreu-Ballester, Juan</creator><creator>Rodero, Marta</creator><creator>Cuéllar, Carmen</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202111</creationdate><title>Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors</title><author>Zamora, Vega ; Carlos Andreu-Ballester, Juan ; Rodero, Marta ; Cuéllar, Carmen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-9eee3a18090c3fbec7936772df2f56edac28325d3a0ccc6801cd00fded1ca8cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agonists</topic><topic>Allergies</topic><topic>Animals</topic><topic>Anisakis - immunology</topic><topic>Anisakis simplex</topic><topic>Antigens</topic><topic>Antigens - immunology</topic><topic>Bone marrow</topic><topic>CD11c antigen</topic><topic>CD80 antigen</topic><topic>Cytokine</topic><topic>Cytokines</topic><topic>Dendritic Cell</topic><topic>Dendritic cells</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>H-2 Antigens</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunomodulation</topic><topic>Immunoregulation</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Innate Immunity</topic><topic>Interleukin 10</topic><topic>Interleukin 12</topic><topic>Interleukin-10 - metabolism</topic><topic>Interleukin-12 - metabolism</topic><topic>Intracellular</topic><topic>Larva - immunology</topic><topic>Lymphocytes T</topic><topic>Major histocompatibility complex</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>MyD88 protein</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Surface markers</topic><topic>TLR2 protein</topic><topic>TLR3 protein</topic><topic>TLR4 protein</topic><topic>TLR5 protein</topic><topic>TLR7 protein</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-like receptors</topic><topic>Toll-Like Receptors - agonists</topic><topic>Toll-Like Receptors - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zamora, Vega</creatorcontrib><creatorcontrib>Carlos Andreu-Ballester, Juan</creatorcontrib><creatorcontrib>Rodero, Marta</creatorcontrib><creatorcontrib>Cuéllar, Carmen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International immunopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zamora, Vega</au><au>Carlos Andreu-Ballester, Juan</au><au>Rodero, Marta</au><au>Cuéllar, Carmen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors</atitle><jtitle>International immunopharmacology</jtitle><addtitle>Int Immunopharmacol</addtitle><date>2021-11</date><risdate>2021</risdate><volume>100</volume><spage>108120</spage><epage>108120</epage><pages>108120-108120</pages><artnum>108120</artnum><issn>1567-5769</issn><eissn>1878-1705</eissn><abstract>[Display omitted]
•Anisakis antigens significantly interact with TLR2, TLR4 and TLR9.•BALB/c mice presented acute-inflammatory responses.•C57BL/6J mice developed discreet and resistant responses.
The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).
In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2–9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA.
Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/Treg response, but also it states A. simplex larval antigens present specific mechanisms to modify the innate response of the host. As allergies share common pathways with the immune response against this particular helminth, our results provide a better understanding into the specific mechanisms of A. simplex allergy related diseases.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>34537480</pmid><doi>10.1016/j.intimp.2021.108120</doi><tpages>1</tpages></addata></record> |
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| subjects | Agonists Allergies Animals Anisakis - immunology Anisakis simplex Antigens Antigens - immunology Bone marrow CD11c antigen CD80 antigen Cytokine Cytokines Dendritic Cell Dendritic cells Enzyme-linked immunosorbent assay Female Flow cytometry H-2 Antigens Immune response Immune system Immunomodulation Immunoregulation Inflammation Inflammatory response Innate Immunity Interleukin 10 Interleukin 12 Interleukin-10 - metabolism Interleukin-12 - metabolism Intracellular Larva - immunology Lymphocytes T Major histocompatibility complex Mice Mice, Inbred BALB C Mice, Inbred C57BL MyD88 protein Proteins Receptors Surface markers TLR2 protein TLR3 protein TLR4 protein TLR5 protein TLR7 protein Toll-Like Receptor 2 Toll-like receptors Toll-Like Receptors - agonists Toll-Like Receptors - immunology |
| title | Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors |
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