Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles
Children with vesicoureteral reflux (VUR) are at an increased risk of recurrent urinary tract infections (UTIs) and renal scarring. Gut microbiota are associated with disease phenotypes, but there has been no study that associates urinary microbiota (uMB) and metabolic profiles with VUR pathology. T...
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| Veröffentlicht in: | European urology 2022-02, Vol.81 (2), p.151-154 |
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| creator | Vitko, Dijana McQuaid, Joseph W. Gheinani, Ali Hashemi Hasegawa, Kohei DiMartino, Shannon Davis, Kylie H. Chung, Candace Y. Petrosino, Joseph F. Adam, Rosalyn M. Mansbach, Jonathan M. Lee, Richard S. |
| description | Children with vesicoureteral reflux (VUR) are at an increased risk of recurrent urinary tract infections (UTIs) and renal scarring. Gut microbiota are associated with disease phenotypes, but there has been no study that associates urinary microbiota (uMB) and metabolic profiles with VUR pathology. To identify dominant uMB genera and metabolites associated with UTIs in VUR, urine samples collected under sterile conditions underwent 16S ribosomal RNA sequencing (n = 49) and metabolomic analysis by mass spectrometry (n = 96). Alterations in uMB and metabolomic profiles in VUR patients suggest remodeling of urinary bacterial communities after UTIs: Dorea- and Escherichia-dominant uMB profiles were more frequently identified in participants with VUR. Prevotella- and Lactobacillus-dominant uMB profiles were more prevalent in controls (p |
| doi_str_mv | 10.1016/j.eururo.2021.08.022 |
| format | Article |
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Differences in urinary commensal bacteria and metabolites exist between children with and without vesicoureteral reflux (VUR). These changes may be utilized to identify patients at risk of VUR-associated kidney damage.
We identified differences in urinary microbiota and metabolic profiles of patients with vesicoureteral reflux (VUR) versus controls. These included alterations in amino acid, energy, lipid, and bile acid metabolism. Such changes persisted in VUR patients even after the resolution of urinary tract infections.</description><identifier>ISSN: 0302-2838</identifier><identifier>EISSN: 1873-7560</identifier><identifier>DOI: 10.1016/j.eururo.2021.08.022</identifier><identifier>PMID: 34538688</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Female ; Fever - complications ; Humans ; Infant ; Male ; Metabolome ; Microbiome ; Microbiota ; Renal scarring ; Urinary tract infection ; Urinary Tract Infections ; Urine ; Vesico-Ureteral Reflux - complications ; Vesicoureteral reflux</subject><ispartof>European urology, 2022-02, Vol.81 (2), p.151-154</ispartof><rights>2021 European Association of Urology</rights><rights>Copyright © 2021 European Association of Urology. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-7229eb264013f5fdcbd47c3baf2ef1dbd73f969ce44e893e8301362b2a7bb75a3</citedby><cites>FETCH-LOGICAL-c362t-7229eb264013f5fdcbd47c3baf2ef1dbd73f969ce44e893e8301362b2a7bb75a3</cites><orcidid>0000-0003-0468-3171 ; 0000-0001-9424-7175</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0302283821019904$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34538688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vitko, Dijana</creatorcontrib><creatorcontrib>McQuaid, Joseph W.</creatorcontrib><creatorcontrib>Gheinani, Ali Hashemi</creatorcontrib><creatorcontrib>Hasegawa, Kohei</creatorcontrib><creatorcontrib>DiMartino, Shannon</creatorcontrib><creatorcontrib>Davis, Kylie H.</creatorcontrib><creatorcontrib>Chung, Candace Y.</creatorcontrib><creatorcontrib>Petrosino, Joseph F.</creatorcontrib><creatorcontrib>Adam, Rosalyn M.</creatorcontrib><creatorcontrib>Mansbach, Jonathan M.</creatorcontrib><creatorcontrib>Lee, Richard S.</creatorcontrib><title>Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles</title><title>European urology</title><addtitle>Eur Urol</addtitle><description>Children with vesicoureteral reflux (VUR) are at an increased risk of recurrent urinary tract infections (UTIs) and renal scarring. Gut microbiota are associated with disease phenotypes, but there has been no study that associates urinary microbiota (uMB) and metabolic profiles with VUR pathology. To identify dominant uMB genera and metabolites associated with UTIs in VUR, urine samples collected under sterile conditions underwent 16S ribosomal RNA sequencing (n = 49) and metabolomic analysis by mass spectrometry (n = 96). Alterations in uMB and metabolomic profiles in VUR patients suggest remodeling of urinary bacterial communities after UTIs: Dorea- and Escherichia-dominant uMB profiles were more frequently identified in participants with VUR. Prevotella- and Lactobacillus-dominant uMB profiles were more prevalent in controls (p < 0.001). Microbial composition varied based on recurrent febrile UTI status (p = 0.001). A total of 243 urinary metabolites involved in energy, amino acid, nucleotide, and lipid metabolism were altered in VUR patients with UTIs (p < 0.05). Importantly, VUR specimens revealed changes in the bacteria-associated metabolic pathways such as glutamate degradation, methyl-citrate cycle, and bile acid metabolism.
Differences in urinary commensal bacteria and metabolites exist between children with and without vesicoureteral reflux (VUR). These changes may be utilized to identify patients at risk of VUR-associated kidney damage.
We identified differences in urinary microbiota and metabolic profiles of patients with vesicoureteral reflux (VUR) versus controls. These included alterations in amino acid, energy, lipid, and bile acid metabolism. Such changes persisted in VUR patients even after the resolution of urinary tract infections.</description><subject>Female</subject><subject>Fever - complications</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Metabolome</subject><subject>Microbiome</subject><subject>Microbiota</subject><subject>Renal scarring</subject><subject>Urinary tract infection</subject><subject>Urinary Tract Infections</subject><subject>Urine</subject><subject>Vesico-Ureteral Reflux - complications</subject><subject>Vesicoureteral reflux</subject><issn>0302-2838</issn><issn>1873-7560</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EokvhHyDkI5cEfyS2c0Farfio1AqEWq6WP8aqV0682Emh_4EfTVbb9shpLs_MOzMPQm8paSmh4sO-haUsJbeMMNoS1RLGnqENVZI3shfkOdoQTljDFFdn6FWte0II7wf-Ep3xrudKKLVBf29KnEy5x9fFuBlfTAHcHPNUcZzw7jYmX2DCv-N8i39CjS4vBWYoJuEfENLyB28L4K1zeTyYKYLH9h5v05F4mvKYcBVdyTbm2WAzeXwFs7E55RHw95JDTFBfoxfBpApvHuo5uvn86Xr3tbn89uVit71sHBdsbiRjA1gmOkJ56IN31nfScWsCg0C99ZKHQQwOug7UwEHxFRTMMiOtlb3h5-j9ae6h5F8L1FmPsTpIyUyQl6pZLzvJJRViRbsTuu5ea4GgDyWO6zmaEn30oPf65EEfPWii9OphbXv3kLDYEfxT0-PjV-DjCYD1zrsIRVcXYXLgY1kNaJ_j_xP-AdJNnq4</recordid><startdate>202202</startdate><enddate>202202</enddate><creator>Vitko, Dijana</creator><creator>McQuaid, Joseph W.</creator><creator>Gheinani, Ali Hashemi</creator><creator>Hasegawa, Kohei</creator><creator>DiMartino, Shannon</creator><creator>Davis, Kylie H.</creator><creator>Chung, Candace Y.</creator><creator>Petrosino, Joseph F.</creator><creator>Adam, Rosalyn M.</creator><creator>Mansbach, Jonathan M.</creator><creator>Lee, Richard S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0468-3171</orcidid><orcidid>https://orcid.org/0000-0001-9424-7175</orcidid></search><sort><creationdate>202202</creationdate><title>Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles</title><author>Vitko, Dijana ; McQuaid, Joseph W. ; Gheinani, Ali Hashemi ; Hasegawa, Kohei ; DiMartino, Shannon ; Davis, Kylie H. ; Chung, Candace Y. ; Petrosino, Joseph F. ; Adam, Rosalyn M. ; Mansbach, Jonathan M. ; Lee, Richard S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-7229eb264013f5fdcbd47c3baf2ef1dbd73f969ce44e893e8301362b2a7bb75a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Female</topic><topic>Fever - complications</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Metabolome</topic><topic>Microbiome</topic><topic>Microbiota</topic><topic>Renal scarring</topic><topic>Urinary tract infection</topic><topic>Urinary Tract Infections</topic><topic>Urine</topic><topic>Vesico-Ureteral Reflux - complications</topic><topic>Vesicoureteral reflux</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vitko, Dijana</creatorcontrib><creatorcontrib>McQuaid, Joseph W.</creatorcontrib><creatorcontrib>Gheinani, Ali Hashemi</creatorcontrib><creatorcontrib>Hasegawa, Kohei</creatorcontrib><creatorcontrib>DiMartino, Shannon</creatorcontrib><creatorcontrib>Davis, Kylie H.</creatorcontrib><creatorcontrib>Chung, Candace Y.</creatorcontrib><creatorcontrib>Petrosino, Joseph F.</creatorcontrib><creatorcontrib>Adam, Rosalyn M.</creatorcontrib><creatorcontrib>Mansbach, Jonathan M.</creatorcontrib><creatorcontrib>Lee, Richard S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European urology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vitko, Dijana</au><au>McQuaid, Joseph W.</au><au>Gheinani, Ali Hashemi</au><au>Hasegawa, Kohei</au><au>DiMartino, Shannon</au><au>Davis, Kylie H.</au><au>Chung, Candace Y.</au><au>Petrosino, Joseph F.</au><au>Adam, Rosalyn M.</au><au>Mansbach, Jonathan M.</au><au>Lee, Richard S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles</atitle><jtitle>European urology</jtitle><addtitle>Eur Urol</addtitle><date>2022-02</date><risdate>2022</risdate><volume>81</volume><issue>2</issue><spage>151</spage><epage>154</epage><pages>151-154</pages><issn>0302-2838</issn><eissn>1873-7560</eissn><abstract>Children with vesicoureteral reflux (VUR) are at an increased risk of recurrent urinary tract infections (UTIs) and renal scarring. Gut microbiota are associated with disease phenotypes, but there has been no study that associates urinary microbiota (uMB) and metabolic profiles with VUR pathology. To identify dominant uMB genera and metabolites associated with UTIs in VUR, urine samples collected under sterile conditions underwent 16S ribosomal RNA sequencing (n = 49) and metabolomic analysis by mass spectrometry (n = 96). Alterations in uMB and metabolomic profiles in VUR patients suggest remodeling of urinary bacterial communities after UTIs: Dorea- and Escherichia-dominant uMB profiles were more frequently identified in participants with VUR. Prevotella- and Lactobacillus-dominant uMB profiles were more prevalent in controls (p < 0.001). Microbial composition varied based on recurrent febrile UTI status (p = 0.001). A total of 243 urinary metabolites involved in energy, amino acid, nucleotide, and lipid metabolism were altered in VUR patients with UTIs (p < 0.05). Importantly, VUR specimens revealed changes in the bacteria-associated metabolic pathways such as glutamate degradation, methyl-citrate cycle, and bile acid metabolism.
Differences in urinary commensal bacteria and metabolites exist between children with and without vesicoureteral reflux (VUR). These changes may be utilized to identify patients at risk of VUR-associated kidney damage.
We identified differences in urinary microbiota and metabolic profiles of patients with vesicoureteral reflux (VUR) versus controls. These included alterations in amino acid, energy, lipid, and bile acid metabolism. Such changes persisted in VUR patients even after the resolution of urinary tract infections.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>34538688</pmid><doi>10.1016/j.eururo.2021.08.022</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-0468-3171</orcidid><orcidid>https://orcid.org/0000-0001-9424-7175</orcidid></addata></record> |
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| ispartof | European urology, 2022-02, Vol.81 (2), p.151-154 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Female Fever - complications Humans Infant Male Metabolome Microbiome Microbiota Renal scarring Urinary tract infection Urinary Tract Infections Urine Vesico-Ureteral Reflux - complications Vesicoureteral reflux |
| title | Urinary Tract Infections in Children with Vesicoureteral Reflux Are Accompanied by Alterations in Urinary Microbiota and Metabolome Profiles |
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