The Vibrio parahaemolyticus subunit toxin PirBvp recognizes glycoproteins on the epithelium of the Penaeus vannamei hepatopancreas

Vibrio parahaemolyticus toxin PirABvp is the major virulence factor exotoxin that contributes to the disruption of the hepatopancreatic epithelium in acute hepatopancreatic necrosis disease in shrimp. The PirBvp subunit is a lectin that recognizes amino sugars; however, its potential role in recogni...

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Veröffentlicht in:Comparative Biochemistry and Physiology, Part B Part B, 2022-01, Vol.257, p.110673-110673, Article 110673
Hauptverfasser: De los Santos, Marcelo Victorio, Sánchez-Salgado, José Luis, Pereyra, Ali, Zenteno, Edgar, Vibanco-Pérez, Norberto, Ramos-Clamont Montfort, Gabriela, Soto-Rodriguez, Sonia A.
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container_title Comparative Biochemistry and Physiology, Part B
container_volume 257
creator De los Santos, Marcelo Victorio
Sánchez-Salgado, José Luis
Pereyra, Ali
Zenteno, Edgar
Vibanco-Pérez, Norberto
Ramos-Clamont Montfort, Gabriela
Soto-Rodriguez, Sonia A.
description Vibrio parahaemolyticus toxin PirABvp is the major virulence factor exotoxin that contributes to the disruption of the hepatopancreatic epithelium in acute hepatopancreatic necrosis disease in shrimp. The PirBvp subunit is a lectin that recognizes amino sugars; however, its potential role in recognition of the hepatopancreas has not been identified. In the present work, we identified the cellular receptor for PirBvp in the shrimp hepatopancreas. A ligand blot assay of hepatopancreas lysate showed that the PirBvp subunit recognizes two glycoprotein bands of 60 and 70 kDa (Gc60 and Gc70). The hepatopancreas lysate was fractionated by anion-exchange chromatography, and the three main fractions obtained contained the recognized Gc60 and Gc70 protein bands. LC-MS/MS indicated that beta-hexosaminidases subunit beta and mucin-like 5 AC corresponded to the 60 and 70 kDa bands, respectively, which seem to be expressed in the epithelial cells of the hepatopancreas. Endoglycosidase treatment of hepatopancreas lysate with the O-glycosidase from Enterococcus faecalis, inhibits the binding of PirBvp. Altogether, these results suggest the relevance of the interaction of PirBvp with the hepatopancreas in the pathogenesis of acute hepatopancreatic necrosis disease in shrimp. [Display omitted] •The PirBvp subunit is a lectin that recognizes amino sugars.•Beta-hexosaminidases subunit beta and mucin-like glycoproteins from shrimp hepatopancreas are recognized by PirBvp subunit.•PirBvp have a pathological importance for acute hepatopancreatic necrosis disease in shrimp.
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The PirBvp subunit is a lectin that recognizes amino sugars; however, its potential role in recognition of the hepatopancreas has not been identified. In the present work, we identified the cellular receptor for PirBvp in the shrimp hepatopancreas. A ligand blot assay of hepatopancreas lysate showed that the PirBvp subunit recognizes two glycoprotein bands of 60 and 70 kDa (Gc60 and Gc70). The hepatopancreas lysate was fractionated by anion-exchange chromatography, and the three main fractions obtained contained the recognized Gc60 and Gc70 protein bands. LC-MS/MS indicated that beta-hexosaminidases subunit beta and mucin-like 5 AC corresponded to the 60 and 70 kDa bands, respectively, which seem to be expressed in the epithelial cells of the hepatopancreas. Endoglycosidase treatment of hepatopancreas lysate with the O-glycosidase from Enterococcus faecalis, inhibits the binding of PirBvp. Altogether, these results suggest the relevance of the interaction of PirBvp with the hepatopancreas in the pathogenesis of acute hepatopancreatic necrosis disease in shrimp. [Display omitted] •The PirBvp subunit is a lectin that recognizes amino sugars.•Beta-hexosaminidases subunit beta and mucin-like glycoproteins from shrimp hepatopancreas are recognized by PirBvp subunit.•PirBvp have a pathological importance for acute hepatopancreatic necrosis disease in shrimp.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2021.110673</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>AHPND ; beta-hexosaminidases subunit beta ; Lectin ; Mucin-like receptor ; PirBvp toxin</subject><ispartof>Comparative Biochemistry and Physiology, Part B, 2022-01, Vol.257, p.110673-110673, Article 110673</ispartof><rights>2021 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1096495921001123$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65308</link.rule.ids></links><search><creatorcontrib>De los Santos, Marcelo Victorio</creatorcontrib><creatorcontrib>Sánchez-Salgado, José Luis</creatorcontrib><creatorcontrib>Pereyra, Ali</creatorcontrib><creatorcontrib>Zenteno, Edgar</creatorcontrib><creatorcontrib>Vibanco-Pérez, Norberto</creatorcontrib><creatorcontrib>Ramos-Clamont Montfort, Gabriela</creatorcontrib><creatorcontrib>Soto-Rodriguez, Sonia A.</creatorcontrib><title>The Vibrio parahaemolyticus subunit toxin PirBvp recognizes glycoproteins on the epithelium of the Penaeus vannamei hepatopancreas</title><title>Comparative Biochemistry and Physiology, Part B</title><description>Vibrio parahaemolyticus toxin PirABvp is the major virulence factor exotoxin that contributes to the disruption of the hepatopancreatic epithelium in acute hepatopancreatic necrosis disease in shrimp. 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Altogether, these results suggest the relevance of the interaction of PirBvp with the hepatopancreas in the pathogenesis of acute hepatopancreatic necrosis disease in shrimp. 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Altogether, these results suggest the relevance of the interaction of PirBvp with the hepatopancreas in the pathogenesis of acute hepatopancreatic necrosis disease in shrimp. [Display omitted] •The PirBvp subunit is a lectin that recognizes amino sugars.•Beta-hexosaminidases subunit beta and mucin-like glycoproteins from shrimp hepatopancreas are recognized by PirBvp subunit.•PirBvp have a pathological importance for acute hepatopancreatic necrosis disease in shrimp.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.cbpb.2021.110673</doi><tpages>1</tpages></addata></record>
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subjects AHPND
beta-hexosaminidases subunit beta
Lectin
Mucin-like receptor
PirBvp toxin
title The Vibrio parahaemolyticus subunit toxin PirBvp recognizes glycoproteins on the epithelium of the Penaeus vannamei hepatopancreas
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