Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt
This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to...
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| Veröffentlicht in: | Journal of infection in developing countries 2021-08, Vol.15 (8), p.1133-1138 |
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| creator | Abd El-Razik, Khaled Abd El-Hamid Arafa, Amany A Fouad, Ehab A Younes, Abdelgayed M Almuzaini, Abdulaziz M Abdou, Amr M |
| description | This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to assess the genetic variation in isolated strains of S. agalactiae.
Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR.
A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively.
The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. |
| doi_str_mv | 10.3855/jidc.12668 |
| format | Article |
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Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR.
A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively.
The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.12668</identifier><identifier>PMID: 34516421</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Animals ; Cattle ; Dairy farms ; Egypt - epidemiology ; Female ; Livestock ; Mastitis, Bovine - diagnosis ; Mastitis, Bovine - epidemiology ; Mastitis, Bovine - microbiology ; Milk ; Milk - microbiology ; Streptococcal Infections - veterinary ; Streptococcus agalactiae - isolation & purification ; Streptococcus infections ; Virulence ; Virulence Factors - genetics</subject><ispartof>Journal of infection in developing countries, 2021-08, Vol.15 (8), p.1133-1138</ispartof><rights>Copyright (c) 2021 Khaled Abd EL-Hamid Abd El-Razik, Amany A Arafa, Ehab A Fouad, Abdelgayed M Younes, Abdulaziz M Almuzaini, Amr M Abdou.</rights><rights>2021. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-538372e9b10b68ea5823a6df23af62fe10bc4443d78611eaf627a22bb754402b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34516421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abd El-Razik, Khaled Abd El-Hamid</creatorcontrib><creatorcontrib>Arafa, Amany A</creatorcontrib><creatorcontrib>Fouad, Ehab A</creatorcontrib><creatorcontrib>Younes, Abdelgayed M</creatorcontrib><creatorcontrib>Almuzaini, Abdulaziz M</creatorcontrib><creatorcontrib>Abdou, Amr M</creatorcontrib><title>Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to assess the genetic variation in isolated strains of S. agalactiae.
Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR.
A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively.
The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt.</description><subject>Animals</subject><subject>Cattle</subject><subject>Dairy farms</subject><subject>Egypt - epidemiology</subject><subject>Female</subject><subject>Livestock</subject><subject>Mastitis, Bovine - diagnosis</subject><subject>Mastitis, Bovine - epidemiology</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Milk</subject><subject>Milk - microbiology</subject><subject>Streptococcal Infections - veterinary</subject><subject>Streptococcus agalactiae - isolation & purification</subject><subject>Streptococcus infections</subject><subject>Virulence</subject><subject>Virulence Factors - genetics</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkV1LwzAUhoMoTqc3_gAJeCNiZ5M0aXspY-pg4IV6XdL0dGS0SU3Swfz1dh-KeHO-eHg58CB0ReIJyzh_WOlKTQgVIjtCZyRPaURFFh__mUfo3PtVHPOccXKKRizhRCSUnKGvubeNDNqae6wrMEHXWu12LE2F19r1DRgFuIIArtVGmuCxrfFbcNAFq6xSvcdyKRupgpaAa2dbXNq1NoB9X6pGmyGxwa30QQftsTZ4ttx04QKd1LLxcHnoY_TxNHufvkSL1-f59HERKUZ4iDjLWEohL0lcigwkzyiToqqHWgtaw3BWSZKwKs0EIbA9ppLSskx5ksS0ZGN0u8_tnP3swYei1V5B00gDtvcF5SnljMWCD-jNP3Rle2eG7_YUoVlOBupuTylnvXdQF53TrXSbgsTF1kixNVLsjAzw9SGyL1uoftEfBewb1S2IUg</recordid><startdate>20210831</startdate><enddate>20210831</enddate><creator>Abd El-Razik, Khaled Abd El-Hamid</creator><creator>Arafa, Amany A</creator><creator>Fouad, Ehab A</creator><creator>Younes, Abdelgayed M</creator><creator>Almuzaini, Abdulaziz M</creator><creator>Abdou, Amr M</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20210831</creationdate><title>Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt</title><author>Abd El-Razik, Khaled Abd El-Hamid ; Arafa, Amany A ; Fouad, Ehab A ; Younes, Abdelgayed M ; Almuzaini, Abdulaziz M ; Abdou, Amr M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-538372e9b10b68ea5823a6df23af62fe10bc4443d78611eaf627a22bb754402b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Dairy farms</topic><topic>Egypt - epidemiology</topic><topic>Female</topic><topic>Livestock</topic><topic>Mastitis, Bovine - diagnosis</topic><topic>Mastitis, Bovine - epidemiology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Streptococcal Infections - veterinary</topic><topic>Streptococcus agalactiae - isolation & purification</topic><topic>Streptococcus infections</topic><topic>Virulence</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abd El-Razik, Khaled Abd El-Hamid</creatorcontrib><creatorcontrib>Arafa, Amany A</creatorcontrib><creatorcontrib>Fouad, Ehab A</creatorcontrib><creatorcontrib>Younes, Abdelgayed M</creatorcontrib><creatorcontrib>Almuzaini, Abdulaziz M</creatorcontrib><creatorcontrib>Abdou, Amr M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Public Health Database (Proquest)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of infection in developing countries</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abd El-Razik, Khaled Abd El-Hamid</au><au>Arafa, Amany A</au><au>Fouad, Ehab A</au><au>Younes, Abdelgayed M</au><au>Almuzaini, Abdulaziz M</au><au>Abdou, Amr M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt</atitle><jtitle>Journal of infection in developing countries</jtitle><addtitle>J Infect Dev Ctries</addtitle><date>2021-08-31</date><risdate>2021</risdate><volume>15</volume><issue>8</issue><spage>1133</spage><epage>1138</epage><pages>1133-1138</pages><issn>1972-2680</issn><issn>2036-6590</issn><eissn>1972-2680</eissn><abstract>This study aimed to investigate the prevalence of contagious mastitis caused by Streptococcus agalctiae (S. agalactiae) in cattle from households and small-scale dairy farms in Egypt. Molecular characterization of S.agalactiae isolates was described including the genetic determinants of virulence to assess the genetic variation in isolated strains of S. agalactiae.
Three hundred and sixty milk samples were collected from 90 apparently healthy dairy cows randomly selected from household and small-scale dairy farms were examined by Somatic Cell Count (SCC) as an indicator for subclinical mastitis. S.agalactiae isolates were bacteriologically and molecularly identified followed by identification of virulence genes using PCR.
A total of 172 milk samples (47.77%) were positive with SCC > 200×103/ml. Bacteriological examination of the positive SCC milk samples revealed that 28 (16.28%) of the isolates were S.agalactiae. Molecular examination using PCR confirmed only 22 isolates of S. agalactiae (12.8%). Moreover, we used the pattern of virulence genes to address the genetic variation of S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt. Virulence genes hylB, cylE, iagA, and bac were determined in 100%, 68.2%, 13.6% and 100% of isolates respectively.
The use of molecular methods for the identification of the causative agent in mastitis confirmed that, in Egypt, Streptococcus agalactiae is considered as one of the predominant infectious agents among contagious mastitis causing pathogens. The pattern of virulence genes presented the genetic diversity of highly virulent S. agalactiae strains isolated from cases of contagious mastitis in cattle in Egypt.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>34516421</pmid><doi>10.3855/jidc.12668</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1972-2680 |
| ispartof | Journal of infection in developing countries, 2021-08, Vol.15 (8), p.1133-1138 |
| issn | 1972-2680 2036-6590 1972-2680 |
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| source | MEDLINE; EZB Electronic Journals Library |
| subjects | Animals Cattle Dairy farms Egypt - epidemiology Female Livestock Mastitis, Bovine - diagnosis Mastitis, Bovine - epidemiology Mastitis, Bovine - microbiology Milk Milk - microbiology Streptococcal Infections - veterinary Streptococcus agalactiae - isolation & purification Streptococcus infections Virulence Virulence Factors - genetics |
| title | Isolation, identification and virulence determinants of Streptococcus agalactiae from bovine subclinical mastitis in Egypt |
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